Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

HAUS augmin-like complex subunit 8

Gene

Haus8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.By similarity

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
HAUS augmin-like complex subunit 8
Alternative name(s):
HEC1/NDC80-interacting centrosome-associated protein 1
Sarcoma antigen NY-SAR-48 homolog
Gene namesi
Name:Haus8
Synonyms:Hice1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1923728 Haus8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003199382 – 373HAUS augmin-like complex subunit 8Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei99PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ99L00
PeptideAtlasiQ99L00
PRIDEiQ99L00

PTM databases

iPTMnetiQ99L00
PhosphoSitePlusiQ99L00

Expressioni

Gene expression databases

BgeeiENSMUSG00000035439
CleanExiMM_2410004L22RIK
ExpressionAtlasiQ99L00 baseline and differential
GenevisibleiQ99L00 MM

Interactioni

Subunit structurei

Component of the HAUS augmin-like complex. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly. Associates with microtubules. The interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. It is unclear whether this interaction is direct or indirect (By similarity).By similarity

Protein-protein interaction databases

BioGridi2181445 interactors.
IntActiQ99L00 5 interactors.
STRINGi10090.ENSMUSP00000040802

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili130 – 204Sequence analysisAdd BLAST75

Sequence similaritiesi

Belongs to the HAUS8 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IEK2 Eukaryota
ENOG4112BWM LUCA
GeneTreeiENSGT00390000010974
HOGENOMiHOG000112854
InParanoidiQ99L00
KOiK16591
OMAiIMCKEKE
OrthoDBiEOG091G0B15
PhylomeDBiQ99L00
TreeFamiTF332998

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99L00-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADSSERDAG KSAAAGACAV PKTKGRRVQG RRVVESRYLQ YDKKAKKVSG
60 70 80 90 100
AAKEEKPPEG RKASTVPRSR EESQVMGTGN LQSTMLEGHG MNPPDLDLSA
110 120 130 140 150
IDDKILSRKA SWPDREMTDK AKSTSFISCD KKRILRKKRR DLQETMDMME
160 170 180 190 200
SQTLLMTLLS VKMENNLALL EERAEKDLAA MCHEKERLQR QALELRRQLL
210 220 230 240 250
LRQKHQELAA TLDAQIEVLS PLQPVLERFK EEYMTLGRAL DTTRHELPMQ
260 270 280 290 300
AVHMEGSGQE LLDDLEPALR ITLQLLGDLS ICSPYATAQV QGASAQEPGA
310 320 330 340 350
STQLSCLLKE LKGLVTEKDL ELRRLVSQVV ELSSQASKEA ALMNQEVWEE
360 370
AEGALTSSQW YFSPDACRDD SPS
Length:373
Mass (Da):41,653
Last modified:February 26, 2008 - v2
Checksum:i9F9896D057BD885E
GO
Isoform 2 (identifier: Q99L00-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     71-71: Missing.

Show »
Length:372
Mass (Da):41,524
Checksum:i1AB41E066AEEA7B4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50G → V in AAH03935 (PubMed:15489334).Curated1
Sequence conflicti259Q → R in BAB26909 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03154471Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010398 mRNA Translation: BAB26909.1
BC003935 mRNA Translation: AAH03935.1
CCDSiCCDS22389.1 [Q99L00-2]
CCDS52579.1 [Q99L00-1]
RefSeqiNP_001156514.1, NM_001163042.1 [Q99L00-1]
NP_083897.2, NM_029621.3 [Q99L00-2]
UniGeneiMm.27355

Genome annotation databases

EnsembliENSMUST00000035960; ENSMUSP00000040802; ENSMUSG00000035439 [Q99L00-1]
ENSMUST00000110071; ENSMUSP00000105698; ENSMUSG00000035439 [Q99L00-2]
GeneIDi76478
KEGGimmu:76478
UCSCiuc009mcg.2 mouse [Q99L00-2]
uc009mch.2 mouse [Q99L00-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHAUS8_MOUSE
AccessioniPrimary (citable) accession number: Q99L00
Secondary accession number(s): Q9CWT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: April 25, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome