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Q99KY4

- GAK_MOUSE

UniProt

Q99KY4 - GAK_MOUSE

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Protein

Cyclin-G-associated kinase

Gene

Gak

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 By similarity.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691ATPPROSITE-ProRule annotation
Active sitei173 – 1731Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 549ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell development Source: MGI
  3. clathrin coat disassembly Source: MGI
  4. clathrin-mediated endocytosis Source: MGI
  5. endoplasmic reticulum organization Source: MGI
  6. epidermal cell differentiation Source: MGI
  7. establishment of skin barrier Source: MGI
  8. forebrain morphogenesis Source: MGI
  9. Golgi organization Source: MGI
  10. intrahepatic bile duct development Source: MGI
  11. positive regulation of neural precursor cell proliferation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-G-associated kinase (EC:2.7.11.1)
Gene namesi
Name:Gak
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:2442153. Gak.

Subcellular locationi

Cytoplasmperinuclear region By similarity. Golgi apparatustrans-Golgi network By similarity. Cell junctionfocal adhesion By similarity
Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions By similarity.By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. Golgi apparatus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 13051304Cyclin-G-associated kinasePRO_0000085959Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei454 – 4541PhosphoserineBy similarity
Modified residuei768 – 7681PhosphoserineBy similarity
Modified residuei824 – 8241PhosphoserineBy similarity
Modified residuei827 – 8271Phosphoserine1 Publication
Modified residuei938 – 9381PhosphoserineBy similarity
Modified residuei1171 – 11711PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ99KY4.
PaxDbiQ99KY4.
PRIDEiQ99KY4.

PTM databases

PhosphoSiteiQ99KY4.

Miscellaneous databases

PMAP-CutDBQ99KY4.

Expressioni

Gene expression databases

BgeeiQ99KY4.
CleanExiMM_GAK.
ExpressionAtlasiQ99KY4. baseline and differential.
GenevestigatoriQ99KY4.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Il12rb2P973787EBI-7652906,EBI-6253448

Protein-protein interaction databases

BioGridi231137. 2 interactions.
IntActiQ99KY4. 2 interactions.
MINTiMINT-1869993.
STRINGi10090.ENSMUSP00000036705.

Structurei

3D structure databases

ProteinModelPortaliQ99KY4.
SMRiQ99KY4. Positions 25-334, 1132-1305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 317278Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini397 – 564168Phosphatase tensin-typePROSITE-ProRule annotationAdd
BLAST
Domaini570 – 708139C2 tensin-typePROSITE-ProRule annotationAdd
BLAST
Domaini1241 – 130565JPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 C2 tensin-type domain.PROSITE-ProRule annotation
Contains 1 J domain.PROSITE-ProRule annotation
Contains 1 phosphatase tensin-type domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119113.
HOGENOMiHOG000034235.
HOVERGENiHBG004322.
InParanoidiQ99KY4.
KOiK08855.
PhylomeDBiQ99KY4.
TreeFamiTF105165.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q99KY4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG
60 70 80 90 100
FAFVYEAQDL GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ
110 120 130 140 150
FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLKRVECK GPLSCDSILK
160 170 180 190 200
IFYQTCRAVQ HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH
210 220 230 240 250
YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI GEKQDIWALG
260 270 280 290 300
CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRAMLKVNP
310 320 330 340 350
VERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPC
360 370 380 390 400
GGTVNSSGVL ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN
410 420 430 440 450
YAKGDLDISY ITSRIAVMSF PAEGVESAIK NNIEDVRMFL DAKHPGHYAV
460 470 480 490 500
YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL HSLYTLCRSM HAWLREDHRN
510 520 530 540 550
VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK RCPPGIWPSH
560 570 580 590 600
KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY
610 620 630 640 650
VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GVTVQGDVLI IIYHARATLG
660 670 680 690 700
GRLQAKMASM KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ
710 720 730 740 750
VNLEVEVEPR DRPSREAPPW ENTSLRGLNP KILFSNREEQ QDILSKFGKP
760 770 780 790 800
ELPRQPGSTA QYDAEAGSPE AEITESDSPQ SSSTDTNHFL HTLDWQEEKE
810 820 830 840 850
PETGLDNTSP KESQSVLIAD GDGSEVSDEE EASFPSEERK PGAGEDTPRL
860 870 880 890 900
AAGTKQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQACG
910 920 930 940 950
VPSSNTDLLS CLLEPSDAAQ VGPPGDLLGG EAPLLLASPV SPLGLQNNLQ
960 970 980 990 1000
GKVPDTVDPF DQFLLSSNSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS
1010 1020 1030 1040 1050
APPPSCSTAF LHLGDLPAEP SKVIASSSHP DLLGGWDTWA DTATPGPASI
1060 1070 1080 1090 1100
PVPEGTLFSS AGHPAPPGPN PSQTKSQNLD PFADLSDLSS SLQGLPAGLP
1110 1120 1130 1140 1150
AGGFVGAPAP TQKSNSPWQA NRPTAPGTSW TPQAKPAPRA SEQLRSHFSV
1160 1170 1180 1190 1200
IGAREERGVR VPSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK
1210 1220 1230 1240 1250
QELARDTDPL KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD
1260 1270 1280 1290 1300
LVTPEQVKKQ YRRAVLVVHP DKATGQPYEQ YAKMIFMELN DAWSEFENQG

SRPLF
Length:1,305
Mass (Da):143,641
Last modified:October 11, 2005 - v2
Checksum:i4FC416FE52650ED2
GO
Isoform 2 (identifier: Q99KY4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     748-796: Missing.

Note: No experimental confirmation available.

Show »
Length:1,256
Mass (Da):138,344
Checksum:i47F3767646BDF6C7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti301 – 3011V → E in BAE38941. (PubMed:16141072)Curated
Sequence conflicti301 – 3011V → E in AAH65052. (PubMed:15489334)Curated
Sequence conflicti822 – 8221D → G in BAC28779. (PubMed:16141072)Curated
Sequence conflicti868 – 8681A → V in AAH03958. (PubMed:15489334)Curated
Sequence conflicti868 – 8681A → V in AAH30859. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei748 – 79649Missing in isoform 2. 1 PublicationVSP_015821Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK034637 mRNA. Translation: BAC28779.1.
AK166682 mRNA. Translation: BAE38941.1.
BC003958 mRNA. Translation: AAH03958.1.
BC030859 mRNA. Translation: AAH30859.1.
BC060622 mRNA. Translation: AAH60622.1.
BC065052 mRNA. Translation: AAH65052.1.
CCDSiCCDS19513.1. [Q99KY4-1]
RefSeqiNP_001268980.1. NM_001282051.1.
NP_001268981.1. NM_001282052.1.
NP_705797.1. NM_153569.2.
UniGeneiMm.276647.

Genome annotation databases

EnsembliENSMUST00000046603; ENSMUSP00000036705; ENSMUSG00000062234.
GeneIDi231580.
KEGGimmu:231580.
UCSCiuc008yoh.1. mouse. [Q99KY4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK034637 mRNA. Translation: BAC28779.1 .
AK166682 mRNA. Translation: BAE38941.1 .
BC003958 mRNA. Translation: AAH03958.1 .
BC030859 mRNA. Translation: AAH30859.1 .
BC060622 mRNA. Translation: AAH60622.1 .
BC065052 mRNA. Translation: AAH65052.1 .
CCDSi CCDS19513.1. [Q99KY4-1 ]
RefSeqi NP_001268980.1. NM_001282051.1.
NP_001268981.1. NM_001282052.1.
NP_705797.1. NM_153569.2.
UniGenei Mm.276647.

3D structure databases

ProteinModelPortali Q99KY4.
SMRi Q99KY4. Positions 25-334, 1132-1305.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 231137. 2 interactions.
IntActi Q99KY4. 2 interactions.
MINTi MINT-1869993.
STRINGi 10090.ENSMUSP00000036705.

PTM databases

PhosphoSitei Q99KY4.

Proteomic databases

MaxQBi Q99KY4.
PaxDbi Q99KY4.
PRIDEi Q99KY4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000046603 ; ENSMUSP00000036705 ; ENSMUSG00000062234 .
GeneIDi 231580.
KEGGi mmu:231580.
UCSCi uc008yoh.1. mouse. [Q99KY4-1 ]

Organism-specific databases

CTDi 2580.
MGIi MGI:2442153. Gak.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000119113.
HOGENOMi HOG000034235.
HOVERGENi HBG004322.
InParanoidi Q99KY4.
KOi K08855.
PhylomeDBi Q99KY4.
TreeFami TF105165.

Miscellaneous databases

NextBioi 380626.
PMAP-CutDB Q99KY4.
PROi Q99KY4.
SOURCEi Search...

Gene expression databases

Bgeei Q99KY4.
CleanExi MM_GAK.
ExpressionAtlasi Q99KY4. baseline and differential.
Genevestigatori Q99KY4.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProi IPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view ]
Pfami PF00226. DnaJ. 1 hit.
PF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view ]
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Blastocyst and Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain, Colon and Mammary tumor.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-827, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGAK_MOUSE
AccessioniPrimary (citable) accession number: Q99KY4
Secondary accession number(s): Q6P1I8
, Q6P9S5, Q8BM74, Q8K0Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: October 11, 2005
Last modified: October 29, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3