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Q99KY4

- GAK_MOUSE

UniProt

Q99KY4 - GAK_MOUSE

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Protein
Cyclin-G-associated kinase
Gene
Gak
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691ATP By similarity
Active sitei173 – 1731Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 549ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. Golgi organization Source: MGI
  2. cell cycle Source: UniProtKB-KW
  3. cell development Source: MGI
  4. clathrin coat disassembly Source: MGI
  5. clathrin-mediated endocytosis Source: MGI
  6. endoplasmic reticulum organization Source: MGI
  7. epidermal cell differentiation Source: MGI
  8. establishment of skin barrier Source: MGI
  9. forebrain morphogenesis Source: MGI
  10. intrahepatic bile duct development Source: MGI
  11. positive regulation of neural precursor cell proliferation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-G-associated kinase (EC:2.7.11.1)
Gene namesi
Name:Gak
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:2442153. Gak.

Subcellular locationi

Cytoplasmperinuclear region By similarity. Golgi apparatustrans-Golgi network By similarity. Cell junctionfocal adhesion By similarity
Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions By similarity.

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-SubCell
  2. focal adhesion Source: UniProtKB-SubCell
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 13051304Cyclin-G-associated kinase
PRO_0000085959Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei16 – 161Phosphoserine By similarity
Modified residuei454 – 4541Phosphoserine By similarity
Modified residuei768 – 7681Phosphoserine By similarity
Modified residuei824 – 8241Phosphoserine By similarity
Modified residuei827 – 8271Phosphoserine1 Publication
Modified residuei938 – 9381Phosphoserine By similarity
Modified residuei1171 – 11711Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ99KY4.
PaxDbiQ99KY4.
PRIDEiQ99KY4.

PTM databases

PhosphoSiteiQ99KY4.

Miscellaneous databases

PMAP-CutDBQ99KY4.

Expressioni

Gene expression databases

ArrayExpressiQ99KY4.
BgeeiQ99KY4.
CleanExiMM_GAK.
GenevestigatoriQ99KY4.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Il12rb2P973787EBI-7652906,EBI-6253448

Protein-protein interaction databases

BioGridi231137. 2 interactions.
IntActiQ99KY4. 1 interaction.
MINTiMINT-1869993.
STRINGi10090.ENSMUSP00000036705.

Structurei

3D structure databases

ProteinModelPortaliQ99KY4.
SMRiQ99KY4. Positions 25-334, 1132-1305.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 317278Protein kinase
Add
BLAST
Domaini397 – 564168Phosphatase tensin-type
Add
BLAST
Domaini570 – 708139C2 tensin-type
Add
BLAST
Domaini1241 – 130565J
Add
BLAST

Sequence similaritiesi

Contains 1 J domain.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00620000087779.
HOGENOMiHOG000034235.
HOVERGENiHBG004322.
InParanoidiQ99KY4.
KOiK08855.
PhylomeDBiQ99KY4.
TreeFamiTF105165.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q99KY4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG     50
FAFVYEAQDL GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ 100
FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLKRVECK GPLSCDSILK 150
IFYQTCRAVQ HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH 200
YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI GEKQDIWALG 250
CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRAMLKVNP 300
VERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPC 350
GGTVNSSGVL ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN 400
YAKGDLDISY ITSRIAVMSF PAEGVESAIK NNIEDVRMFL DAKHPGHYAV 450
YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL HSLYTLCRSM HAWLREDHRN 500
VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK RCPPGIWPSH 550
KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY 600
VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GVTVQGDVLI IIYHARATLG 650
GRLQAKMASM KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ 700
VNLEVEVEPR DRPSREAPPW ENTSLRGLNP KILFSNREEQ QDILSKFGKP 750
ELPRQPGSTA QYDAEAGSPE AEITESDSPQ SSSTDTNHFL HTLDWQEEKE 800
PETGLDNTSP KESQSVLIAD GDGSEVSDEE EASFPSEERK PGAGEDTPRL 850
AAGTKQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQACG 900
VPSSNTDLLS CLLEPSDAAQ VGPPGDLLGG EAPLLLASPV SPLGLQNNLQ 950
GKVPDTVDPF DQFLLSSNSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS 1000
APPPSCSTAF LHLGDLPAEP SKVIASSSHP DLLGGWDTWA DTATPGPASI 1050
PVPEGTLFSS AGHPAPPGPN PSQTKSQNLD PFADLSDLSS SLQGLPAGLP 1100
AGGFVGAPAP TQKSNSPWQA NRPTAPGTSW TPQAKPAPRA SEQLRSHFSV 1150
IGAREERGVR VPSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK 1200
QELARDTDPL KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD 1250
LVTPEQVKKQ YRRAVLVVHP DKATGQPYEQ YAKMIFMELN DAWSEFENQG 1300
SRPLF 1305
Length:1,305
Mass (Da):143,641
Last modified:October 11, 2005 - v2
Checksum:i4FC416FE52650ED2
GO
Isoform 2 (identifier: Q99KY4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     748-796: Missing.

Note: No experimental confirmation available.

Show »
Length:1,256
Mass (Da):138,344
Checksum:i47F3767646BDF6C7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei748 – 79649Missing in isoform 2.
VSP_015821Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti301 – 3011V → E in BAE38941. 1 Publication
Sequence conflicti301 – 3011V → E in AAH65052. 1 Publication
Sequence conflicti822 – 8221D → G in BAC28779. 1 Publication
Sequence conflicti868 – 8681A → V in AAH03958. 1 Publication
Sequence conflicti868 – 8681A → V in AAH30859. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK034637 mRNA. Translation: BAC28779.1.
AK166682 mRNA. Translation: BAE38941.1.
BC003958 mRNA. Translation: AAH03958.1.
BC030859 mRNA. Translation: AAH30859.1.
BC060622 mRNA. Translation: AAH60622.1.
BC065052 mRNA. Translation: AAH65052.1.
CCDSiCCDS19513.1. [Q99KY4-1]
RefSeqiNP_001268980.1. NM_001282051.1.
NP_001268981.1. NM_001282052.1.
NP_705797.1. NM_153569.2.
UniGeneiMm.276647.

Genome annotation databases

EnsembliENSMUST00000046603; ENSMUSP00000036705; ENSMUSG00000062234.
GeneIDi231580.
KEGGimmu:231580.
UCSCiuc008yoh.1. mouse. [Q99KY4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK034637 mRNA. Translation: BAC28779.1 .
AK166682 mRNA. Translation: BAE38941.1 .
BC003958 mRNA. Translation: AAH03958.1 .
BC030859 mRNA. Translation: AAH30859.1 .
BC060622 mRNA. Translation: AAH60622.1 .
BC065052 mRNA. Translation: AAH65052.1 .
CCDSi CCDS19513.1. [Q99KY4-1 ]
RefSeqi NP_001268980.1. NM_001282051.1.
NP_001268981.1. NM_001282052.1.
NP_705797.1. NM_153569.2.
UniGenei Mm.276647.

3D structure databases

ProteinModelPortali Q99KY4.
SMRi Q99KY4. Positions 25-334, 1132-1305.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 231137. 2 interactions.
IntActi Q99KY4. 1 interaction.
MINTi MINT-1869993.
STRINGi 10090.ENSMUSP00000036705.

PTM databases

PhosphoSitei Q99KY4.

Proteomic databases

MaxQBi Q99KY4.
PaxDbi Q99KY4.
PRIDEi Q99KY4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000046603 ; ENSMUSP00000036705 ; ENSMUSG00000062234 .
GeneIDi 231580.
KEGGi mmu:231580.
UCSCi uc008yoh.1. mouse. [Q99KY4-1 ]

Organism-specific databases

CTDi 2580.
MGIi MGI:2442153. Gak.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00620000087779.
HOGENOMi HOG000034235.
HOVERGENi HBG004322.
InParanoidi Q99KY4.
KOi K08855.
PhylomeDBi Q99KY4.
TreeFami TF105165.

Miscellaneous databases

NextBioi 380626.
PMAP-CutDB Q99KY4.
PROi Q99KY4.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q99KY4.
Bgeei Q99KY4.
CleanExi MM_GAK.
Genevestigatori Q99KY4.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProi IPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view ]
Pfami PF00226. DnaJ. 1 hit.
PF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view ]
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Blastocyst and Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain, Colon and Mammary tumor.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-827, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGAK_MOUSE
AccessioniPrimary (citable) accession number: Q99KY4
Secondary accession number(s): Q6P1I8
, Q6P9S5, Q8BM74, Q8K0Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: October 11, 2005
Last modified: September 3, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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