Q99KY4 (GAK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-G-associated kinase
EC=2.7.11.1

Gene names

Name:

Gak

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm › perinuclear region By similarity. Golgi apparatus › trans-Golgi network By similarity. Cell junction › focal adhesion By similarity. Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 C2 tensin-type domain. Contains 1 J domain. Contains 1 phosphatase tensin-type domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cell junction Cytoplasm Golgi apparatus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell focal adhesion Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99KY4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99KY4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     748-796: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 1305	1304	Cyclin-G-associated kinase		PRO_0000085959
Regions
	Domain	40 – 317	278	Protein kinase
	Domain	397 – 564	168	Phosphatase tensin-type
	Domain	570 – 708	139	C2 tensin-type
	Domain	1241 – 1305	65	J
	Nucleotide binding	46 – 54	9	ATP By similarity
Sites
	Active site	173	1	Proton acceptor By similarity
	Binding site	69	1	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	16	1	Phosphoserine By similarity
	Modified residue	454	1	Phosphoserine By similarity
	Modified residue	768	1	Phosphoserine By similarity
	Modified residue	824	1	Phosphoserine By similarity
	Modified residue	827	1	Phosphoserine Ref.3
	Modified residue	938	1	Phosphoserine By similarity
	Modified residue	1171	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	748 – 796	49	Missing in isoform 2.		VSP_015821
Experimental info
	Sequence conflict	301	1	V → E in BAE38941. Ref.1
	Sequence conflict	301	1	V → E in AAH65052. Ref.2
	Sequence conflict	822	1	D → G in BAC28779. Ref.1
	Sequence conflict	868	1	A → V in AAH03958. Ref.2
	Sequence conflict	868	1	A → V in AAH30859. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2005. Version 2. Checksum: 4FC416FE52650ED2	FASTA	1,305	143,641
	10 20 30 40 50 60 MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG FAFVYEAQDL 70 80 90 100 110 120 GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ FCSAASIGKE ESDTGQAEFL 130 140 150 160 170 180 LLTELCKGQL VEFLKRVECK GPLSCDSILK IFYQTCRAVQ HMHRQKPPII HRDLKVENLL 190 200 210 220 230 240 LSNQGTIKLC DFGSATTISH YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI 250 260 270 280 290 300 GEKQDIWALG CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRAMLKVNP 310 320 330 340 350 360 VERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPC GGTVNSSGVL 370 380 390 400 410 420 ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN YAKGDLDISY ITSRIAVMSF 430 440 450 460 470 480 PAEGVESAIK NNIEDVRMFL DAKHPGHYAV YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL 490 500 510 520 530 540 HSLYTLCRSM HAWLREDHRN VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK 550 560 570 580 590 600 RCPPGIWPSH KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY 610 620 630 640 650 660 VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GVTVQGDVLI IIYHARATLG GRLQAKMASM 670 680 690 700 710 720 KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ VNLEVEVEPR DRPSREAPPW 730 740 750 760 770 780 ENTSLRGLNP KILFSNREEQ QDILSKFGKP ELPRQPGSTA QYDAEAGSPE AEITESDSPQ 790 800 810 820 830 840 SSSTDTNHFL HTLDWQEEKE PETGLDNTSP KESQSVLIAD GDGSEVSDEE EASFPSEERK 850 860 870 880 890 900 PGAGEDTPRL AAGTKQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQACG 910 920 930 940 950 960 VPSSNTDLLS CLLEPSDAAQ VGPPGDLLGG EAPLLLASPV SPLGLQNNLQ GKVPDTVDPF 970 980 990 1000 1010 1020 DQFLLSSNSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS APPPSCSTAF LHLGDLPAEP 1030 1040 1050 1060 1070 1080 SKVIASSSHP DLLGGWDTWA DTATPGPASI PVPEGTLFSS AGHPAPPGPN PSQTKSQNLD 1090 1100 1110 1120 1130 1140 PFADLSDLSS SLQGLPAGLP AGGFVGAPAP TQKSNSPWQA NRPTAPGTSW TPQAKPAPRA 1150 1160 1170 1180 1190 1200 SEQLRSHFSV IGAREERGVR VPSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK 1210 1220 1230 1240 1250 1260 QELARDTDPL KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD LVTPEQVKKQ 1270 1280 1290 1300 YRRAVLVVHP DKATGQPYEQ YAKMIFMELN DAWSEFENQG SRPLF
	Isoform 2 [UniParc]. Checksum: 47F3767646BDF6C7	FASTA	1,256	138,344
	10 20 30 40 50 60 MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG FAFVYEAQDL 70 80 90 100 110 120 GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ FCSAASIGKE ESDTGQAEFL 130 140 150 160 170 180 LLTELCKGQL VEFLKRVECK GPLSCDSILK IFYQTCRAVQ HMHRQKPPII HRDLKVENLL 190 200 210 220 230 240 LSNQGTIKLC DFGSATTISH YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI 250 260 270 280 290 300 GEKQDIWALG CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRAMLKVNP 310 320 330 340 350 360 VERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPC GGTVNSSGVL 370 380 390 400 410 420 ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN YAKGDLDISY ITSRIAVMSF 430 440 450 460 470 480 PAEGVESAIK NNIEDVRMFL DAKHPGHYAV YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL 490 500 510 520 530 540 HSLYTLCRSM HAWLREDHRN VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK 550 560 570 580 590 600 RCPPGIWPSH KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY 610 620 630 640 650 660 VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GVTVQGDVLI IIYHARATLG GRLQAKMASM 670 680 690 700 710 720 KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ VNLEVEVEPR DRPSREAPPW 730 740 750 760 770 780 ENTSLRGLNP KILFSNREEQ QDILSKFEEK EPETGLDNTS PKESQSVLIA DGDGSEVSDE 790 800 810 820 830 840 EEASFPSEER KPGAGEDTPR LAAGTKQQDL IFDVGMLAAP QEPVQPEEGV DLLGLHSEGD 850 860 870 880 890 900 LRPAAPLQAC GVPSSNTDLL SCLLEPSDAA QVGPPGDLLG GEAPLLLASP VSPLGLQNNL 910 920 930 940 950 960 QGKVPDTVDP FDQFLLSSNS DTQPCSKPDL FGEFLNSDSV ASSTAFPSTH SAPPPSCSTA 970 980 990 1000 1010 1020 FLHLGDLPAE PSKVIASSSH PDLLGGWDTW ADTATPGPAS IPVPEGTLFS SAGHPAPPGP 1030 1040 1050 1060 1070 1080 NPSQTKSQNL DPFADLSDLS SSLQGLPAGL PAGGFVGAPA PTQKSNSPWQ ANRPTAPGTS 1090 1100 1110 1120 1130 1140 WTPQAKPAPR ASEQLRSHFS VIGAREERGV RVPSFAQKPK VSENDFEDLL PNQGFSKSDK 1150 1160 1170 1180 1190 1200 KGPKTMAEMR KQELARDTDP LKLKLLDWIE GKERNIRALL STLHTVLWDG ESRWTPVSMA 1210 1220 1230 1240 1250 DLVTPEQVKK QYRRAVLVVH PDKATGQPYE QYAKMIFMEL NDAWSEFENQ GSRPLF

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Blastocyst and Embryo.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6 and FVB/N. Tissue: Brain, Colon and Mammary tumor.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-827, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK034637 mRNA. Translation: BAC28779.1. AK166682 mRNA. Translation: BAE38941.1. BC003958 mRNA. Translation: AAH03958.1. BC030859 mRNA. Translation: AAH30859.1. BC060622 mRNA. Translation: AAH60622.1. BC065052 mRNA. Translation: AAH65052.1.
IPI	IPI00420480. IPI00620823.
RefSeq	NP_705797.1. NM_153569.1.
UniGene	Mm.276647.
3D structure databases
ProteinModelPortal	Q99KY4.
SMR	Q99KY4. Positions 28-336, 398-744, 1132-1305.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1869993.
STRING	10090.ENSMUSP00000036705.
PTM databases
PhosphoSite	Q99KY4.
Proteomic databases
PaxDb	Q99KY4.
PRIDE	Q99KY4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000046603; ENSMUSP00000036705; ENSMUSG00000062234.
GeneID	231580.
KEGG	mmu:231580.
Organism-specific databases
CTD	2580.
MGI	MGI:2442153. Gak.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00620000087779.
HOGENOM	HOG000034235.
HOVERGEN	HBG004322.
InParanoid	Q99KY4.
KO	K08855.
OrthoDB	EOG44MXR7.
Gene expression databases
ArrayExpress	Q99KY4.
Bgee	Q99KY4.
CleanEx	MM_GAK.
Genevestigator	Q99KY4.
GermOnline	ENSMUSG00000062234. Mus musculus.
Family and domain databases
Gene3D	1.10.287.110. 1 hit.
InterPro	IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR001623. DnaJ_domain. IPR011009. Kinase-like_dom. IPR014019. Phosphatase_tensin-typ. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. IPR014020. Tensin_phosphatase_C2-dom. [Graphical view]
Pfam	PF00226. DnaJ. 1 hit. PF00069. Pkinase. 1 hit. PF10409. PTEN_C2. 1 hit. [Graphical view]
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF46565. DnaJ_N. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS51182. C2_TENSIN. 1 hit. PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. PS51181. PPASE_TENSIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	380626.
PMAP-CutDB	Q99KY4.
SOURCE	Search...

Entry information

Entry name

GAK_MOUSE

Accession

Primary (citable) accession number: Q99KY4
Secondary accession number(s): Q6P1I8 , Q6P9S5, Q8BM74, Q8K0Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	October 11, 2005
Last modified:	April 3, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program