Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q99KY4 (GAK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-G-associated kinase

EC=2.7.11.1
Gene names
Name:Gak
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1305 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Cytoplasmperinuclear region By similarity. Golgi apparatustrans-Golgi network By similarity. Cell junctionfocal adhesion By similarity. Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 C2 tensin-type domain.

Contains 1 J domain.

Contains 1 phosphatase tensin-type domain.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Il12rb2P973787EBI-7652906,EBI-6253448

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99KY4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99KY4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     748-796: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 13051304Cyclin-G-associated kinase
PRO_0000085959

Regions

Domain40 – 317278Protein kinase
Domain397 – 564168Phosphatase tensin-type
Domain570 – 708139C2 tensin-type
Domain1241 – 130565J
Nucleotide binding46 – 549ATP By similarity

Sites

Active site1731Proton acceptor By similarity
Binding site691ATP By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue161Phosphoserine By similarity
Modified residue4541Phosphoserine By similarity
Modified residue7681Phosphoserine By similarity
Modified residue8241Phosphoserine By similarity
Modified residue8271Phosphoserine Ref.3
Modified residue9381Phosphoserine By similarity
Modified residue11711Phosphoserine By similarity

Natural variations

Alternative sequence748 – 79649Missing in isoform 2.
VSP_015821

Experimental info

Sequence conflict3011V → E in BAE38941. Ref.1
Sequence conflict3011V → E in AAH65052. Ref.2
Sequence conflict8221D → G in BAC28779. Ref.1
Sequence conflict8681A → V in AAH03958. Ref.2
Sequence conflict8681A → V in AAH30859. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 11, 2005. Version 2.
Checksum: 4FC416FE52650ED2

FASTA1,305143,641
        10         20         30         40         50         60 
MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG FAFVYEAQDL 

        70         80         90        100        110        120 
GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ FCSAASIGKE ESDTGQAEFL 

       130        140        150        160        170        180 
LLTELCKGQL VEFLKRVECK GPLSCDSILK IFYQTCRAVQ HMHRQKPPII HRDLKVENLL 

       190        200        210        220        230        240 
LSNQGTIKLC DFGSATTISH YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI 

       250        260        270        280        290        300 
GEKQDIWALG CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRAMLKVNP 

       310        320        330        340        350        360 
VERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPC GGTVNSSGVL 

       370        380        390        400        410        420 
ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN YAKGDLDISY ITSRIAVMSF 

       430        440        450        460        470        480 
PAEGVESAIK NNIEDVRMFL DAKHPGHYAV YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL 

       490        500        510        520        530        540 
HSLYTLCRSM HAWLREDHRN VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK 

       550        560        570        580        590        600 
RCPPGIWPSH KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY 

       610        620        630        640        650        660 
VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GVTVQGDVLI IIYHARATLG GRLQAKMASM 

       670        680        690        700        710        720 
KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ VNLEVEVEPR DRPSREAPPW 

       730        740        750        760        770        780 
ENTSLRGLNP KILFSNREEQ QDILSKFGKP ELPRQPGSTA QYDAEAGSPE AEITESDSPQ 

       790        800        810        820        830        840 
SSSTDTNHFL HTLDWQEEKE PETGLDNTSP KESQSVLIAD GDGSEVSDEE EASFPSEERK 

       850        860        870        880        890        900 
PGAGEDTPRL AAGTKQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQACG 

       910        920        930        940        950        960 
VPSSNTDLLS CLLEPSDAAQ VGPPGDLLGG EAPLLLASPV SPLGLQNNLQ GKVPDTVDPF 

       970        980        990       1000       1010       1020 
DQFLLSSNSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS APPPSCSTAF LHLGDLPAEP 

      1030       1040       1050       1060       1070       1080 
SKVIASSSHP DLLGGWDTWA DTATPGPASI PVPEGTLFSS AGHPAPPGPN PSQTKSQNLD 

      1090       1100       1110       1120       1130       1140 
PFADLSDLSS SLQGLPAGLP AGGFVGAPAP TQKSNSPWQA NRPTAPGTSW TPQAKPAPRA 

      1150       1160       1170       1180       1190       1200 
SEQLRSHFSV IGAREERGVR VPSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK 

      1210       1220       1230       1240       1250       1260 
QELARDTDPL KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD LVTPEQVKKQ 

      1270       1280       1290       1300 
YRRAVLVVHP DKATGQPYEQ YAKMIFMELN DAWSEFENQG SRPLF 

« Hide

Isoform 2 [UniParc].

Checksum: 47F3767646BDF6C7
Show »

FASTA1,256138,344

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Blastocyst and Embryo.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6 and FVB/N.
Tissue: Brain, Colon and Mammary tumor.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-827, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK034637 mRNA. Translation: BAC28779.1.
AK166682 mRNA. Translation: BAE38941.1.
BC003958 mRNA. Translation: AAH03958.1.
BC030859 mRNA. Translation: AAH30859.1.
BC060622 mRNA. Translation: AAH60622.1.
BC065052 mRNA. Translation: AAH65052.1.
CCDSCCDS19513.1. [Q99KY4-1]
RefSeqNP_001268980.1. NM_001282051.1.
NP_001268981.1. NM_001282052.1.
NP_705797.1. NM_153569.2.
UniGeneMm.276647.

3D structure databases

ProteinModelPortalQ99KY4.
SMRQ99KY4. Positions 25-334, 1132-1305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid231137. 2 interactions.
IntActQ99KY4. 1 interaction.
MINTMINT-1869993.
STRING10090.ENSMUSP00000036705.

PTM databases

PhosphoSiteQ99KY4.

Proteomic databases

MaxQBQ99KY4.
PaxDbQ99KY4.
PRIDEQ99KY4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000046603; ENSMUSP00000036705; ENSMUSG00000062234.
GeneID231580.
KEGGmmu:231580.
UCSCuc008yoh.1. mouse. [Q99KY4-1]

Organism-specific databases

CTD2580.
MGIMGI:2442153. Gak.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00620000087779.
HOGENOMHOG000034235.
HOVERGENHBG004322.
InParanoidQ99KY4.
KOK08855.
PhylomeDBQ99KY4.
TreeFamTF105165.

Gene expression databases

ArrayExpressQ99KY4.
BgeeQ99KY4.
CleanExMM_GAK.
GenevestigatorQ99KY4.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
3.90.190.10. 1 hit.
InterProIPR000008. C2_dom.
IPR001623. DnaJ_domain.
IPR011009. Kinase-like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR014020. Tensin_C2-dom.
IPR029023. Tensin_lipid_phosphatase_dom.
[Graphical view]
PfamPF00226. DnaJ. 1 hit.
PF00069. Pkinase. 1 hit.
PF10409. PTEN_C2. 1 hit.
[Graphical view]
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51182. C2_TENSIN. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51181. PPASE_TENSIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio380626.
PMAP-CutDBQ99KY4.
PROQ99KY4.
SOURCESearch...

Entry information

Entry nameGAK_MOUSE
AccessionPrimary (citable) accession number: Q99KY4
Secondary accession number(s): Q6P1I8 expand/collapse secondary AC list , Q6P9S5, Q8BM74, Q8K0Q4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot