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Q99KY4

- GAK_MOUSE

UniProt

Q99KY4 - GAK_MOUSE

Protein

Cyclin-G-associated kinase

Gene

Gak

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691ATPPROSITE-ProRule annotation
    Active sitei173 – 1731Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi46 – 549ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell development Source: MGI
    3. clathrin coat disassembly Source: MGI
    4. clathrin-mediated endocytosis Source: MGI
    5. endoplasmic reticulum organization Source: MGI
    6. epidermal cell differentiation Source: MGI
    7. establishment of skin barrier Source: MGI
    8. forebrain morphogenesis Source: MGI
    9. Golgi organization Source: MGI
    10. intrahepatic bile duct development Source: MGI
    11. positive regulation of neural precursor cell proliferation Source: MGI

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-G-associated kinase (EC:2.7.11.1)
    Gene namesi
    Name:Gak
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:2442153. Gak.

    Subcellular locationi

    Cytoplasmperinuclear region By similarity. Golgi apparatustrans-Golgi network By similarity. Cell junctionfocal adhesion By similarity
    Note: Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions By similarity.By similarity

    GO - Cellular componenti

    1. focal adhesion Source: UniProtKB-SubCell
    2. Golgi apparatus Source: UniProtKB-SubCell
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 13051304Cyclin-G-associated kinasePRO_0000085959Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei16 – 161PhosphoserineBy similarity
    Modified residuei454 – 4541PhosphoserineBy similarity
    Modified residuei768 – 7681PhosphoserineBy similarity
    Modified residuei824 – 8241PhosphoserineBy similarity
    Modified residuei827 – 8271Phosphoserine1 Publication
    Modified residuei938 – 9381PhosphoserineBy similarity
    Modified residuei1171 – 11711PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ99KY4.
    PaxDbiQ99KY4.
    PRIDEiQ99KY4.

    PTM databases

    PhosphoSiteiQ99KY4.

    Miscellaneous databases

    PMAP-CutDBQ99KY4.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99KY4.
    BgeeiQ99KY4.
    CleanExiMM_GAK.
    GenevestigatoriQ99KY4.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Il12rb2P973787EBI-7652906,EBI-6253448

    Protein-protein interaction databases

    BioGridi231137. 2 interactions.
    IntActiQ99KY4. 2 interactions.
    MINTiMINT-1869993.
    STRINGi10090.ENSMUSP00000036705.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99KY4.
    SMRiQ99KY4. Positions 25-334, 1132-1305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 317278Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini397 – 564168Phosphatase tensin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini570 – 708139C2 tensin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini1241 – 130565JPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 C2 tensin-type domain.PROSITE-ProRule annotation
    Contains 1 J domain.PROSITE-ProRule annotation
    Contains 1 phosphatase tensin-type domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00620000087779.
    HOGENOMiHOG000034235.
    HOVERGENiHBG004322.
    InParanoidiQ99KY4.
    KOiK08855.
    PhylomeDBiQ99KY4.
    TreeFamiTF105165.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR000008. C2_dom.
    IPR001623. DnaJ_domain.
    IPR011009. Kinase-like_dom.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR014020. Tensin_C2-dom.
    IPR029023. Tensin_lipid_phosphatase_dom.
    [Graphical view]
    PfamiPF00226. DnaJ. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF10409. PTEN_C2. 1 hit.
    [Graphical view]
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF52799. SSF52799. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51182. C2_TENSIN. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51181. PPASE_TENSIN. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99KY4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLLQSALDF LAGPGSLGGA AGRDQSDFVG QTVELGELRL RVRRVLAEGG     50
    FAFVYEAQDL GSGREYALKR LLSNEEEKNR AIIQEVCFLK KLSGHPNIVQ 100
    FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLKRVECK GPLSCDSILK 150
    IFYQTCRAVQ HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH 200
    YPDYSWSAQK RAMVEEEITR NTTPMYRTPE IVDLYSNFPI GEKQDIWALG 250
    CILYLLCFRQ HPFEDGAKLR IVNGKYSIPV NDTRYTVFHD LIRAMLKVNP 300
    VERLSIAEVV RQLQEIAAAR NVNPKAPITE LLEQNGGYGN SGPSRAQPPC 350
    GGTVNSSGVL ALAEYDQPYG GFLDILRGGT ERLFTNLKDT SSKVIQSVAN 400
    YAKGDLDISY ITSRIAVMSF PAEGVESAIK NNIEDVRMFL DAKHPGHYAV 450
    YNLSPRIYRA SKFHNRVTEC GWAVRRAPHL HSLYTLCRSM HAWLREDHRN 500
    VCVVHCMDGR AASAVAVCAF LCFCRLFSTA EAAVYMFSMK RCPPGIWPSH 550
    KRYIEYVCDM VAEEPITPHS KPMLVKSVVM TPVPLFSKQR NGCRPFCEVY 600
    VGEERVTTTS QEYDRMKEFK IEDGKAVIPL GVTVQGDVLI IIYHARATLG 650
    GRLQAKMASM KMFQIQFHTG FVPRNATTVK FAKYDLDACD IQEKYPDLFQ 700
    VNLEVEVEPR DRPSREAPPW ENTSLRGLNP KILFSNREEQ QDILSKFGKP 750
    ELPRQPGSTA QYDAEAGSPE AEITESDSPQ SSSTDTNHFL HTLDWQEEKE 800
    PETGLDNTSP KESQSVLIAD GDGSEVSDEE EASFPSEERK PGAGEDTPRL 850
    AAGTKQQDLI FDVGMLAAPQ EPVQPEEGVD LLGLHSEGDL RPAAPLQACG 900
    VPSSNTDLLS CLLEPSDAAQ VGPPGDLLGG EAPLLLASPV SPLGLQNNLQ 950
    GKVPDTVDPF DQFLLSSNSD TQPCSKPDLF GEFLNSDSVA SSTAFPSTHS 1000
    APPPSCSTAF LHLGDLPAEP SKVIASSSHP DLLGGWDTWA DTATPGPASI 1050
    PVPEGTLFSS AGHPAPPGPN PSQTKSQNLD PFADLSDLSS SLQGLPAGLP 1100
    AGGFVGAPAP TQKSNSPWQA NRPTAPGTSW TPQAKPAPRA SEQLRSHFSV 1150
    IGAREERGVR VPSFAQKPKV SENDFEDLLP NQGFSKSDKK GPKTMAEMRK 1200
    QELARDTDPL KLKLLDWIEG KERNIRALLS TLHTVLWDGE SRWTPVSMAD 1250
    LVTPEQVKKQ YRRAVLVVHP DKATGQPYEQ YAKMIFMELN DAWSEFENQG 1300
    SRPLF 1305
    Length:1,305
    Mass (Da):143,641
    Last modified:October 11, 2005 - v2
    Checksum:i4FC416FE52650ED2
    GO
    Isoform 2 (identifier: Q99KY4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         748-796: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,256
    Mass (Da):138,344
    Checksum:i47F3767646BDF6C7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti301 – 3011V → E in BAE38941. (PubMed:16141072)Curated
    Sequence conflicti301 – 3011V → E in AAH65052. (PubMed:15489334)Curated
    Sequence conflicti822 – 8221D → G in BAC28779. (PubMed:16141072)Curated
    Sequence conflicti868 – 8681A → V in AAH03958. (PubMed:15489334)Curated
    Sequence conflicti868 – 8681A → V in AAH30859. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei748 – 79649Missing in isoform 2. 1 PublicationVSP_015821Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK034637 mRNA. Translation: BAC28779.1.
    AK166682 mRNA. Translation: BAE38941.1.
    BC003958 mRNA. Translation: AAH03958.1.
    BC030859 mRNA. Translation: AAH30859.1.
    BC060622 mRNA. Translation: AAH60622.1.
    BC065052 mRNA. Translation: AAH65052.1.
    CCDSiCCDS19513.1. [Q99KY4-1]
    RefSeqiNP_001268980.1. NM_001282051.1.
    NP_001268981.1. NM_001282052.1.
    NP_705797.1. NM_153569.2.
    UniGeneiMm.276647.

    Genome annotation databases

    EnsembliENSMUST00000046603; ENSMUSP00000036705; ENSMUSG00000062234.
    GeneIDi231580.
    KEGGimmu:231580.
    UCSCiuc008yoh.1. mouse. [Q99KY4-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK034637 mRNA. Translation: BAC28779.1 .
    AK166682 mRNA. Translation: BAE38941.1 .
    BC003958 mRNA. Translation: AAH03958.1 .
    BC030859 mRNA. Translation: AAH30859.1 .
    BC060622 mRNA. Translation: AAH60622.1 .
    BC065052 mRNA. Translation: AAH65052.1 .
    CCDSi CCDS19513.1. [Q99KY4-1 ]
    RefSeqi NP_001268980.1. NM_001282051.1.
    NP_001268981.1. NM_001282052.1.
    NP_705797.1. NM_153569.2.
    UniGenei Mm.276647.

    3D structure databases

    ProteinModelPortali Q99KY4.
    SMRi Q99KY4. Positions 25-334, 1132-1305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 231137. 2 interactions.
    IntActi Q99KY4. 2 interactions.
    MINTi MINT-1869993.
    STRINGi 10090.ENSMUSP00000036705.

    PTM databases

    PhosphoSitei Q99KY4.

    Proteomic databases

    MaxQBi Q99KY4.
    PaxDbi Q99KY4.
    PRIDEi Q99KY4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000046603 ; ENSMUSP00000036705 ; ENSMUSG00000062234 .
    GeneIDi 231580.
    KEGGi mmu:231580.
    UCSCi uc008yoh.1. mouse. [Q99KY4-1 ]

    Organism-specific databases

    CTDi 2580.
    MGIi MGI:2442153. Gak.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00620000087779.
    HOGENOMi HOG000034235.
    HOVERGENi HBG004322.
    InParanoidi Q99KY4.
    KOi K08855.
    PhylomeDBi Q99KY4.
    TreeFami TF105165.

    Miscellaneous databases

    NextBioi 380626.
    PMAP-CutDB Q99KY4.
    PROi Q99KY4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99KY4.
    Bgeei Q99KY4.
    CleanExi MM_GAK.
    Genevestigatori Q99KY4.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR000008. C2_dom.
    IPR001623. DnaJ_domain.
    IPR011009. Kinase-like_dom.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR014020. Tensin_C2-dom.
    IPR029023. Tensin_lipid_phosphatase_dom.
    [Graphical view ]
    Pfami PF00226. DnaJ. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF10409. PTEN_C2. 1 hit.
    [Graphical view ]
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF52799. SSF52799. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51182. C2_TENSIN. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51181. PPASE_TENSIN. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Blastocyst and Embryo.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain, Colon and Mammary tumor.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-827, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiGAK_MOUSE
    AccessioniPrimary (citable) accession number: Q99KY4
    Secondary accession number(s): Q6P1I8
    , Q6P9S5, Q8BM74, Q8K0Q4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 29, 2001
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3