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Protein

DnaJ homolog subfamily B member 11

Gene

Dnajb11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity (By similarity).By similarity

GO - Molecular functioni

  • unfolded protein binding Source: MGI

GO - Biological processi

  • mRNA modification Source: MGI
  • positive regulation of ATPase activity Source: MGI
  • protein folding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 11
Alternative name(s):
APOBEC1-binding protein 2
Short name:
ABBP-2
ER-associated DNAJ
ER-associated Hsp40 co-chaperone
Endoplasmic reticulum DNA J domain-containing protein 3
Short name:
ER-resident protein ERdj3
Short name:
ERdj3
Short name:
ERj3p
Gene namesi
Name:Dnajb11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1915088. Dnajb11.

Subcellular locationi

  • Endoplasmic reticulum lumen 1 Publication

GO - Cellular componenti

  • cytoplasm Source: MGI
  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum chaperone complex Source: Ensembl
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • membrane Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000000726123 – 358DnaJ homolog subfamily B member 11Add BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188PhosphothreonineBy similarity1
Glycosylationi261N-linked (GlcNAc...)Curated1

Post-translational modificationi

Contains high-mannose Endo H-sensitive carbohydrates.By similarity
Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ99KV1.
MaxQBiQ99KV1.
PaxDbiQ99KV1.
PeptideAtlasiQ99KV1.
PRIDEiQ99KV1.

2D gel databases

REPRODUCTION-2DPAGEQ99KV1.

PTM databases

iPTMnetiQ99KV1.
PhosphoSitePlusiQ99KV1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004460.
CleanExiMM_DNAJB11.
GenevisibleiQ99KV1. MM.

Interactioni

Subunit structurei

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Binds to denatured substrates in an ATP-independent manner. Interacts via the J domain with HSPA5 in an ATP-dependent manner (By similarity).By similarity

GO - Molecular functioni

  • unfolded protein binding Source: MGI

Protein-protein interaction databases

BioGridi212468. 2 interactors.
DIPiDIP-60531N.
IntActiQ99KV1. 8 interactors.
MINTiMINT-1847287.
STRINGi10090.ENSMUSP00000004574.

Structurei

3D structure databases

ProteinModelPortaliQ99KV1.
SMRiQ99KV1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 90JPROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0713. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00860000133716.
HOGENOMiHOG000226718.
HOVERGENiHBG066727.
InParanoidiQ99KV1.
KOiK09517.
OMAiRDEMEYP.
OrthoDBiEOG091G0GWF.
PhylomeDBiQ99KV1.
TreeFamiTF105144.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99KV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPQNLSTFC LLLLYLIGTV IAGRDFYKIL GVPRSASIKD IKKAYRKLAL
60 70 80 90 100
QLHPDRNPDD PQAQEKFQDL GAAYEVLSDS EKRKQYDTYG EEGLKDGHQS
110 120 130 140 150
SHGDIFSHFF GDFGFMFGGT PRQQDRNIPR GSDIIVDLEV TLEEVYAGNF
160 170 180 190 200
VEVVRNKPVA RQAPGKRKCN CRQEMRTTQL GPGRFQMTQE VVCDECPNVK
210 220 230 240 250
LVNEERTLEV EIEPGVRDGM EYPFIGEGEP HVDGEPGDLR FRIKVVKHRI
260 270 280 290 300
FERRGDDLYT NVTVSLVEAL VGFEMDITHL DGHKVHISRD KITRPGAKLW
310 320 330 340 350
KKGEGLPNFD NNNIKGSLII TFDVDFPKEQ LTEEAKEGIK QLLKQGPVQK

VYNGLQGY
Length:358
Mass (Da):40,555
Last modified:June 1, 2001 - v1
Checksum:iE53CE0B3DF7465B8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9F → L in BAC35956 (PubMed:16141072).Curated1
Sequence conflicti88T → A in AAH18282 (PubMed:15489334).Curated1
Sequence conflicti217R → L in AAL17676 (PubMed:11584023).Curated1
Sequence conflicti240R → P in AAL17676 (PubMed:11584023).Curated1
Sequence conflicti255G → E in BAC35956 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY054981 mRNA. Translation: AAL17676.1.
AK050500 mRNA. Translation: BAC34293.1.
AK075785 mRNA. Translation: BAC35956.1.
AK075953 mRNA. Translation: BAC36079.1.
AK148511 mRNA. Translation: BAE28593.1.
AK166730 mRNA. Translation: BAE38977.1.
CT027991 Genomic DNA. Translation: CAO78022.1.
BC003999 mRNA. Translation: AAH03999.1.
BC018282 mRNA. Translation: AAH18282.1.
BC040747 mRNA. Translation: AAH40747.1.
CCDSiCCDS28070.1.
RefSeqiNP_001177733.1. NM_001190804.1.
NP_001177734.1. NM_001190805.1.
NP_080676.3. NM_026400.5.
UniGeneiMm.37516.
Mm.477367.

Genome annotation databases

EnsembliENSMUST00000004574; ENSMUSP00000004574; ENSMUSG00000004460.
ENSMUST00000166487; ENSMUSP00000126828; ENSMUSG00000004460.
ENSMUST00000178320; ENSMUSP00000137542; ENSMUSG00000004460.
GeneIDi67838.
KEGGimmu:67838.
UCSCiuc007yso.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY054981 mRNA. Translation: AAL17676.1.
AK050500 mRNA. Translation: BAC34293.1.
AK075785 mRNA. Translation: BAC35956.1.
AK075953 mRNA. Translation: BAC36079.1.
AK148511 mRNA. Translation: BAE28593.1.
AK166730 mRNA. Translation: BAE38977.1.
CT027991 Genomic DNA. Translation: CAO78022.1.
BC003999 mRNA. Translation: AAH03999.1.
BC018282 mRNA. Translation: AAH18282.1.
BC040747 mRNA. Translation: AAH40747.1.
CCDSiCCDS28070.1.
RefSeqiNP_001177733.1. NM_001190804.1.
NP_001177734.1. NM_001190805.1.
NP_080676.3. NM_026400.5.
UniGeneiMm.37516.
Mm.477367.

3D structure databases

ProteinModelPortaliQ99KV1.
SMRiQ99KV1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212468. 2 interactors.
DIPiDIP-60531N.
IntActiQ99KV1. 8 interactors.
MINTiMINT-1847287.
STRINGi10090.ENSMUSP00000004574.

PTM databases

iPTMnetiQ99KV1.
PhosphoSitePlusiQ99KV1.

2D gel databases

REPRODUCTION-2DPAGEQ99KV1.

Proteomic databases

EPDiQ99KV1.
MaxQBiQ99KV1.
PaxDbiQ99KV1.
PeptideAtlasiQ99KV1.
PRIDEiQ99KV1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004574; ENSMUSP00000004574; ENSMUSG00000004460.
ENSMUST00000166487; ENSMUSP00000126828; ENSMUSG00000004460.
ENSMUST00000178320; ENSMUSP00000137542; ENSMUSG00000004460.
GeneIDi67838.
KEGGimmu:67838.
UCSCiuc007yso.2. mouse.

Organism-specific databases

CTDi51726.
MGIiMGI:1915088. Dnajb11.

Phylogenomic databases

eggNOGiKOG0713. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00860000133716.
HOGENOMiHOG000226718.
HOVERGENiHBG066727.
InParanoidiQ99KV1.
KOiK09517.
OMAiRDEMEYP.
OrthoDBiEOG091G0GWF.
PhylomeDBiQ99KV1.
TreeFamiTF105144.

Miscellaneous databases

PROiQ99KV1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004460.
CleanExiMM_DNAJB11.
GenevisibleiQ99KV1. MM.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDJB11_MOUSE
AccessioniPrimary (citable) accession number: Q99KV1
Secondary accession number(s): A6X957
, Q543I7, Q8BK79, Q8VCY1, Q8VHB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

PubMed:11584023 reported a cytosolic, as well as nuclear subcellular location. This result was obtained using an N-terminally GFP-tagged construct which most probably affected signal peptide-driven targeting to the ER. As a consequence, the in vivo revelance of the observed interaction with APOBEC1, a nuclear protein, is dubious.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.