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Q99KU1 (DHDDS_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dehydrodolichyl diphosphate synthase

Short name=Dedol-PP synthase
EC=2.5.1.-
Alternative name(s):
Cis-isoprenyltransferase
Short name=CIT
Short name=Cis-IPTase
Gene names
Name:Dhdds
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier-lipid required for the biosynthesis of several classes of glycoprotein. HAMAP-Rule MF_01139

Pathway

Protein modification; protein glycosylation. HAMAP-Rule MF_01139

Subunit structure

Interacts with NUS1/NgBR, the interaction is required for efficient activity. Interacts with NPC2 By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Note: colocalizes with calnexin By similarity. HAMAP-Rule MF_01139

Sequence similarities

Belongs to the UPP synthase family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
   Coding sequence diversityAlternative splicing
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein glycosylation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontransferase activity, transferring alkyl or aryl (other than methyl) groups

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99KU1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99KU1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     255-255: Q → QQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 333333Dehydrodolichyl diphosphate synthase HAMAP-Rule MF_01139
PRO_0000123750

Natural variations

Alternative sequence2551Q → QQ in isoform 2.
VSP_010032

Experimental info

Sequence conflict301A → L in BAC29643. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 53992F5E92D3F2C9

FASTA33338,509
        10         20         30         40         50         60 
MSWIKEGELS LWERFCANII KAGPVPKHIA FIMDGNRRYA KKCQVERQEG HTQGFNKLAE 

        70         80         90        100        110        120 
TLRWCLNLGI LEVTVYAFSI ENFKRSKSEV DGLLDLARQK FSCLMEEQEK LQKHGVCIRV 

       130        140        150        160        170        180 
LGDLHLLPLD LQEKIAHAIQ ATKNYNKCFL NVCFAYTSRH EIANAVREMA WGVEQGLLEP 

       190        200        210        220        230        240 
SDVSESLLDK CLYSNHSPHP DILIRTSGEV RLSDFLLWQT SHSCLVFQPV LWPEYTFWNL 

       250        260        270        280        290        300 
CEAILQFQRN HGALQKARDM YAEERKRRQL ERDQAAVTEQ LLREGLQASG DAQLRRTRLH 

       310        320        330 
KLSTKREERV QGFLKALELK RANWLALWGT ASA 

« Hide

Isoform 2 [UniParc].

Checksum: B4574B527D0D8C1B
Show »

FASTA33438,637

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J and NOD.
Tissue: Diencephalon, Hippocampus, Olfactory bulb, Sympathetic ganglion and Vagina.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK034277 mRNA. Translation: BAC28657.1.
AK036929 mRNA. Translation: BAC29643.1.
AK049991 mRNA. Translation: BAC34020.1.
AK135087 mRNA. Translation: BAE22416.1.
AK149138 mRNA. Translation: BAE28748.1.
AK171207 mRNA. Translation: BAE42313.1.
AL837508, AL670680 Genomic DNA. Translation: CAM46083.1.
BC004011 mRNA. Translation: AAH04011.1.
RefSeqNP_080420.2. NM_026144.4.
UniGeneMm.100290.

3D structure databases

ProteinModelPortalQ99KU1.
SMRQ99KU1. Positions 27-235.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000101511.

PTM databases

PhosphoSiteQ99KU1.

Proteomic databases

PRIDEQ99KU1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000012262; ENSMUSP00000012262; ENSMUSG00000012117. [Q99KU1-1]
ENSMUST00000105887; ENSMUSP00000101511; ENSMUSG00000012117. [Q99KU1-2]
ENSMUST00000144668; ENSMUSP00000116098; ENSMUSG00000012117. [Q99KU1-1]
GeneID67422.
KEGGmmu:67422.
UCSCuc008vds.1. mouse. [Q99KU1-1]
uc008vdv.1. mouse. [Q99KU1-2]

Organism-specific databases

CTD79947.
MGIMGI:1914672. Dhdds.

Phylogenomic databases

eggNOGCOG0020.
GeneTreeENSGT00390000007879.
HOGENOMHOG000006053.
HOVERGENHBG051350.
InParanoidA3KGL4.
KOK11778.
OMAYGGQQEI.
OrthoDBEOG7HTHHB.
PhylomeDBQ99KU1.
TreeFamTF323753.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ99KU1.
BgeeQ99KU1.
CleanExMM_DHDDS.
GenevestigatorQ99KU1.

Family and domain databases

Gene3D3.40.1180.10. 1 hit.
HAMAPMF_01139. ISPT.
InterProIPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERPTHR10291. PTHR10291. 1 hit.
PfamPF01255. Prenyltransf. 1 hit.
[Graphical view]
SUPFAMSSF64005. SSF64005. 1 hit.
TIGRFAMsTIGR00055. uppS. 1 hit.
PROSITEPS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio324524.
PROQ99KU1.
SOURCESearch...

Entry information

Entry nameDHDDS_MOUSE
AccessionPrimary (citable) accession number: Q99KU1
Secondary accession number(s): A3KGL4 expand/collapse secondary AC list , Q3UF13, Q8BZ16, Q8BZK8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot