Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dehydrodolichyl diphosphate syntase complex subunit Dhdds

Gene

Dhdds

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

With DHDDS, forms the dehydrodolichyl diphosphate syntase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Adds multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precusrosor of dolichol which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol.By similarity

Catalytic activityi

(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10-55).By similarity

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_272160. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrodolichyl diphosphate syntase complex subunit DhddsCurated (EC:2.5.1.87By similarity)
Alternative name(s):
Cis-isoprenyltransferase
Short name:
CIT
Short name:
Cis-IPTase
Gene namesi
Name:DhddsImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1914672. Dhdds.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity

  • Note: colocalizes with calnexin.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Dehydrodolichyl diphosphate syntase complex subunit DhddsPRO_0000123750Add
BLAST

Proteomic databases

MaxQBiQ99KU1.
PRIDEiQ99KU1.

PTM databases

PhosphoSiteiQ99KU1.

Expressioni

Gene expression databases

BgeeiQ99KU1.
CleanExiMM_DHDDS.
ExpressionAtlasiQ99KU1. baseline and differential.
GenevisibleiQ99KU1. MM.

Interactioni

Subunit structurei

Forms an active dehydrodolichyl diphosphate syntase complex with NUS1. Interacts with NPC2.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000012262.

Structurei

3D structure databases

ProteinModelPortaliQ99KU1.
SMRiQ99KU1. Positions 25-251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UPP synthase family.Curated

Phylogenomic databases

eggNOGiCOG0020.
GeneTreeiENSGT00390000007879.
HOGENOMiHOG000006053.
HOVERGENiHBG051350.
InParanoidiQ99KU1.
KOiK11778.
OMAiYGGQQEI.
OrthoDBiEOG7HTHHB.
PhylomeDBiQ99KU1.
TreeFamiTF323753.

Family and domain databases

Gene3Di3.40.1180.10. 1 hit.
HAMAPiMF_01139. ISPT.
InterProiIPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERiPTHR10291. PTHR10291. 1 hit.
PfamiPF01255. Prenyltransf. 1 hit.
[Graphical view]
SUPFAMiSSF64005. SSF64005. 1 hit.
TIGRFAMsiTIGR00055. uppS. 1 hit.
PROSITEiPS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99KU1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSWIKEGELS LWERFCANII KAGPVPKHIA FIMDGNRRYA KKCQVERQEG
60 70 80 90 100
HTQGFNKLAE TLRWCLNLGI LEVTVYAFSI ENFKRSKSEV DGLLDLARQK
110 120 130 140 150
FSCLMEEQEK LQKHGVCIRV LGDLHLLPLD LQEKIAHAIQ ATKNYNKCFL
160 170 180 190 200
NVCFAYTSRH EIANAVREMA WGVEQGLLEP SDVSESLLDK CLYSNHSPHP
210 220 230 240 250
DILIRTSGEV RLSDFLLWQT SHSCLVFQPV LWPEYTFWNL CEAILQFQRN
260 270 280 290 300
HGALQKARDM YAEERKRRQL ERDQAAVTEQ LLREGLQASG DAQLRRTRLH
310 320 330
KLSTKREERV QGFLKALELK RANWLALWGT ASA
Length:333
Mass (Da):38,509
Last modified:June 1, 2001 - v1
Checksum:i53992F5E92D3F2C9
GO
Isoform 2 (identifier: Q99KU1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     255-255: Q → QQ

Show »
Length:334
Mass (Da):38,637
Checksum:iB4574B527D0D8C1B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301A → L in BAC29643 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei255 – 2551Q → QQ in isoform 2. 1 PublicationVSP_010032

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK034277 mRNA. Translation: BAC28657.1.
AK036929 mRNA. Translation: BAC29643.1.
AK049991 mRNA. Translation: BAC34020.1.
AK135087 mRNA. Translation: BAE22416.1.
AK149138 mRNA. Translation: BAE28748.1.
AK171207 mRNA. Translation: BAE42313.1.
AL837508, AL670680 Genomic DNA. Translation: CAM46083.1.
BC004011 mRNA. Translation: AAH04011.1.
CCDSiCCDS18760.1. [Q99KU1-1]
RefSeqiNP_080420.2. NM_026144.4. [Q99KU1-1]
UniGeneiMm.100290.

Genome annotation databases

EnsembliENSMUST00000012262; ENSMUSP00000012262; ENSMUSG00000012117. [Q99KU1-1]
ENSMUST00000105887; ENSMUSP00000101511; ENSMUSG00000012117. [Q99KU1-2]
ENSMUST00000144668; ENSMUSP00000116098; ENSMUSG00000012117. [Q99KU1-1]
GeneIDi67422.
KEGGimmu:67422.
UCSCiuc008vds.1. mouse. [Q99KU1-1]
uc008vdv.1. mouse. [Q99KU1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK034277 mRNA. Translation: BAC28657.1.
AK036929 mRNA. Translation: BAC29643.1.
AK049991 mRNA. Translation: BAC34020.1.
AK135087 mRNA. Translation: BAE22416.1.
AK149138 mRNA. Translation: BAE28748.1.
AK171207 mRNA. Translation: BAE42313.1.
AL837508, AL670680 Genomic DNA. Translation: CAM46083.1.
BC004011 mRNA. Translation: AAH04011.1.
CCDSiCCDS18760.1. [Q99KU1-1]
RefSeqiNP_080420.2. NM_026144.4. [Q99KU1-1]
UniGeneiMm.100290.

3D structure databases

ProteinModelPortaliQ99KU1.
SMRiQ99KU1. Positions 25-251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000012262.

PTM databases

PhosphoSiteiQ99KU1.

Proteomic databases

MaxQBiQ99KU1.
PRIDEiQ99KU1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000012262; ENSMUSP00000012262; ENSMUSG00000012117. [Q99KU1-1]
ENSMUST00000105887; ENSMUSP00000101511; ENSMUSG00000012117. [Q99KU1-2]
ENSMUST00000144668; ENSMUSP00000116098; ENSMUSG00000012117. [Q99KU1-1]
GeneIDi67422.
KEGGimmu:67422.
UCSCiuc008vds.1. mouse. [Q99KU1-1]
uc008vdv.1. mouse. [Q99KU1-2]

Organism-specific databases

CTDi79947.
MGIiMGI:1914672. Dhdds.

Phylogenomic databases

eggNOGiCOG0020.
GeneTreeiENSGT00390000007879.
HOGENOMiHOG000006053.
HOVERGENiHBG051350.
InParanoidiQ99KU1.
KOiK11778.
OMAiYGGQQEI.
OrthoDBiEOG7HTHHB.
PhylomeDBiQ99KU1.
TreeFamiTF323753.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_272160. Synthesis of Dolichyl-phosphate.

Miscellaneous databases

NextBioi324524.
PROiQ99KU1.
SOURCEiSearch...

Gene expression databases

BgeeiQ99KU1.
CleanExiMM_DHDDS.
ExpressionAtlasiQ99KU1. baseline and differential.
GenevisibleiQ99KU1. MM.

Family and domain databases

Gene3Di3.40.1180.10. 1 hit.
HAMAPiMF_01139. ISPT.
InterProiIPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERiPTHR10291. PTHR10291. 1 hit.
PfamiPF01255. Prenyltransf. 1 hit.
[Graphical view]
SUPFAMiSSF64005. SSF64005. 1 hit.
TIGRFAMsiTIGR00055. uppS. 1 hit.
PROSITEiPS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Diencephalon, Hippocampus, Olfactory bulb, Sympathetic ganglion and Vagina.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Mammary tumor.

Entry informationi

Entry nameiDHDDS_MOUSE
AccessioniPrimary (citable) accession number: Q99KU1
Secondary accession number(s): A3KGL4
, Q3UF13, Q8BZ16, Q8BZK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.