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Q99KP6

- PRP19_MOUSE

UniProt

Q99KP6 - PRP19_MOUSE

Protein

Pre-mRNA-processing factor 19

Gene

Prpf19

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing By similarity.By similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: MGI
    3. ubiquitin-ubiquitin ligase activity Source: Ensembl

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW
    2. inner cell mass cell proliferation Source: MGI
    3. lipid biosynthetic process Source: MGI
    4. mRNA splicing, via spliceosome Source: UniProtKB
    5. negative regulation of neuron differentiation Source: MGI
    6. positive regulation of astrocyte differentiation Source: MGI
    7. positive regulation of mRNA splicing, via spliceosome Source: MGI
    8. positive regulation of neuron differentiation Source: MGI
    9. protein polyubiquitination Source: Ensembl
    10. spliceosomal complex assembly Source: Ensembl

    Keywords - Biological processi

    DNA damage, DNA repair, mRNA processing, mRNA splicing

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-processing factor 19
    Alternative name(s):
    Nuclear matrix protein 200
    PRP19/PSO4 homolog
    Senescence evasion factor
    Gene namesi
    Name:Prpf19
    Synonyms:Prp19, Snev
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:106247. Prpf19.

    Subcellular locationi

    Nucleus By similarity. Nucleusnucleoplasm By similarity. Cytoplasmcytoskeletonspindle By similarity
    Note: Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. lipid particle Source: MGI
    3. nuclear speck Source: Ensembl
    4. nucleus Source: MGI
    5. Prp19 complex Source: UniProtKB
    6. spindle Source: UniProtKB-SubCell
    7. spliceosomal complex Source: MGI
    8. ubiquitin ligase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 504503Pre-mRNA-processing factor 19PRO_0000051146Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei122 – 1221N6-acetyllysineBy similarity
    Modified residuei179 – 1791N6-acetyllysine1 Publication
    Modified residuei244 – 2441N6-acetyllysine1 Publication
    Modified residuei261 – 2611N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ99KP6.
    PaxDbiQ99KP6.
    PRIDEiQ99KP6.

    2D gel databases

    REPRODUCTION-2DPAGEQ99KP6.

    PTM databases

    PhosphoSiteiQ99KP6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99KP6.
    BgeeiQ99KP6.
    GenevestigatoriQ99KP6.

    Interactioni

    Subunit structurei

    Homooligomer By similarity. Identified in the spliceosome C complex. Component of the PSO4 complex, composed of PRPF19, CDC5L, PLRG1. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts in the complex directly with CDC5L, PLRG1 and BCAS2. Interacts with APEX1, DNTT and PSMB4 By similarity. Interacts with CWC22 and EIF4A3 in an RNA-independent manner By similarity.By similarity

    Protein-protein interaction databases

    BioGridi205709. 2 interactions.
    IntActiQ99KP6. 4 interactions.
    MINTiMINT-1867830.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99KP6.
    SMRiQ99KP6. Positions 3-55, 195-504.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 7372U-boxAdd
    BLAST
    Repeati219 – 25941WD 1Add
    BLAST
    Repeati262 – 30140WD 2Add
    BLAST
    Repeati304 – 34542WD 3Add
    BLAST
    Repeati348 – 38740WD 4Add
    BLAST
    Repeati390 – 42940WD 5Add
    BLAST
    Repeati433 – 47240WD 6Add
    BLAST
    Repeati473 – 50331WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat PRP19 family.Curated
    Contains 1 U-box domain.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00740000115057.
    HOVERGENiHBG053697.
    InParanoidiQ99KP6.
    KOiK10599.
    PhylomeDBiQ99KP6.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    3.30.40.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR013915. Pre-mRNA_splic_Prp19.
    IPR000772. Ricin_B_lectin.
    IPR003613. Ubox_domain.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF08606. Prp19. 1 hit.
    PF04564. U-box. 1 hit.
    PF00400. WD40. 5 hits.
    [Graphical view]
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00504. Ubox. 1 hit.
    SM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS51698. U_BOX. 1 hit.
    PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 4 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99KP6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE    50
    QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHSF TLRQQLQTTR 100
    QELSHALYQH DAACRVIARL TKEVTAAREA LATLKPQAGL IVPQAVPSSQ 150
    PSVVGAGEPM DLGELVGMTP EIIQKLQDKA TVLTTERKKR GKTVPEELVK 200
    PEELSKYRQV ASHVGLHSAS IPGILALDLC PSDTNKILTG GADKNVVVFD 250
    KSTEQILATL KGHTKKVTSV VFHPSQELVF SASPDATIRI WSVPNTSCVQ 300
    VVRAHESAVT GLSLHATGDY LLSSSDDQYW AFSDIQTGRV LTKVTDETSG 350
    CSLTCAQFHP DGLIFGTGTM DSQIKIWDLK ERTNVANFPG HSGPITSIAF 400
    SENGYYLATA ADDSSVKLWD LRKLKNFKTL QLDNNFEVKS LIFDQSGTYL 450
    ALGGTDVQIY ICKQWTEILH FTEHSGLTTG VAFGHHAKFI ASTGMDRSLK 500
    FYSL 504
    Length:504
    Mass (Da):55,239
    Last modified:June 1, 2001 - v1
    Checksum:iB2918188A0651024
    GO
    Isoform 2 (identifier: Q99KP6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         82-82: W → WGWPNSPALPQSSQWPTLSQ

    Note: No experimental confirmation available. Dubious isoform.

    Show »
    Length:523
    Mass (Da):57,302
    Checksum:i4867008E9F3D969E
    GO
    Isoform 3 (identifier: Q99KP6-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2-85: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:420
    Mass (Da):45,920
    Checksum:i9F95F645523FFB19
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei2 – 8584Missing in isoform 3. 1 PublicationVSP_012192Add
    BLAST
    Alternative sequencei82 – 821W → WGWPNSPALPQSSQWPTLSQ in isoform 2. 1 PublicationVSP_011945

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF251503 mRNA. Translation: AAK49039.1.
    AF386760 Genomic DNA. Translation: AAM21468.1.
    AK047693 mRNA. Translation: BAC33127.1.
    AK076998 mRNA. Translation: BAC36557.1.
    AK088769 mRNA. Translation: BAC40560.1.
    AK164681 mRNA. Translation: BAE37873.1.
    BC004070 mRNA. Translation: AAH04070.1.
    CCDSiCCDS57135.1. [Q99KP6-2]
    RefSeqiNP_001240772.1. NM_001253843.1. [Q99KP6-2]
    NP_001240773.1. NM_001253844.1.
    NP_598890.1. NM_134129.4. [Q99KP6-1]
    UniGeneiMm.491104.

    Genome annotation databases

    EnsembliENSMUST00000025642; ENSMUSP00000025642; ENSMUSG00000024735. [Q99KP6-2]
    ENSMUST00000178868; ENSMUSP00000137435; ENSMUSG00000024735.
    GeneIDi28000.
    KEGGimmu:28000.
    UCSCiuc008grf.2. mouse. [Q99KP6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF251503 mRNA. Translation: AAK49039.1 .
    AF386760 Genomic DNA. Translation: AAM21468.1 .
    AK047693 mRNA. Translation: BAC33127.1 .
    AK076998 mRNA. Translation: BAC36557.1 .
    AK088769 mRNA. Translation: BAC40560.1 .
    AK164681 mRNA. Translation: BAE37873.1 .
    BC004070 mRNA. Translation: AAH04070.1 .
    CCDSi CCDS57135.1. [Q99KP6-2 ]
    RefSeqi NP_001240772.1. NM_001253843.1. [Q99KP6-2 ]
    NP_001240773.1. NM_001253844.1.
    NP_598890.1. NM_134129.4. [Q99KP6-1 ]
    UniGenei Mm.491104.

    3D structure databases

    ProteinModelPortali Q99KP6.
    SMRi Q99KP6. Positions 3-55, 195-504.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 205709. 2 interactions.
    IntActi Q99KP6. 4 interactions.
    MINTi MINT-1867830.

    PTM databases

    PhosphoSitei Q99KP6.

    2D gel databases

    REPRODUCTION-2DPAGE Q99KP6.

    Proteomic databases

    MaxQBi Q99KP6.
    PaxDbi Q99KP6.
    PRIDEi Q99KP6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025642 ; ENSMUSP00000025642 ; ENSMUSG00000024735 . [Q99KP6-2 ]
    ENSMUST00000178868 ; ENSMUSP00000137435 ; ENSMUSG00000024735 .
    GeneIDi 28000.
    KEGGi mmu:28000.
    UCSCi uc008grf.2. mouse. [Q99KP6-1 ]

    Organism-specific databases

    CTDi 27339.
    MGIi MGI:106247. Prpf19.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00740000115057.
    HOVERGENi HBG053697.
    InParanoidi Q99KP6.
    KOi K10599.
    PhylomeDBi Q99KP6.

    Miscellaneous databases

    NextBioi 306504.
    PROi Q99KP6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99KP6.
    Bgeei Q99KP6.
    Genevestigatori Q99KP6.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    3.30.40.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR013915. Pre-mRNA_splic_Prp19.
    IPR000772. Ricin_B_lectin.
    IPR003613. Ubox_domain.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF08606. Prp19. 1 hit.
    PF04564. U-box. 1 hit.
    PF00400. WD40. 5 hits.
    [Graphical view ]
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00504. Ubox. 1 hit.
    SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS51698. U_BOX. 1 hit.
    PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 4 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of mSNEV, the mouse homologue of the human nuclear matrix protein NMP200."
      Grillari J., Gross S., Katinger H.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: Swiss albino.
    2. Fortschegger K., Grillari J., Katinger H.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
      Strain: 129/SvJ.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
      Strain: C57BL/6J and NOD.
      Tissue: Corpus striatum, Stomach, Testis and Thymus.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: Czech II.
      Tissue: Mammary gland.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-179 AND LYS-244, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiPRP19_MOUSE
    AccessioniPrimary (citable) accession number: Q99KP6
    Secondary accession number(s): Q3TP64, Q8BKZ5, Q8BVQ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3