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Q99KP6

- PRP19_MOUSE

UniProt

Q99KP6 - PRP19_MOUSE

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Protein
Pre-mRNA-processing factor 19
Gene
Prpf19, Prp19, Snev
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing By similarity.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. protein binding Source: MGI
  3. ubiquitin-ubiquitin ligase activity Source: Ensembl

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. inner cell mass cell proliferation Source: MGI
  3. lipid biosynthetic process Source: MGI
  4. mRNA splicing, via spliceosome Source: UniProtKB
  5. negative regulation of neuron differentiation Source: MGI
  6. positive regulation of astrocyte differentiation Source: MGI
  7. positive regulation of mRNA splicing, via spliceosome Source: MGI
  8. positive regulation of neuron differentiation Source: MGI
  9. protein polyubiquitination Source: Ensembl
  10. spliceosomal complex assembly Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, mRNA processing, mRNA splicing

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 19
Alternative name(s):
Nuclear matrix protein 200
PRP19/PSO4 homolog
Senescence evasion factor
Gene namesi
Name:Prpf19
Synonyms:Prp19, Snev
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:106247. Prpf19.

Subcellular locationi

Nucleus By similarity. Nucleusnucleoplasm By similarity. Cytoplasmcytoskeletonspindle By similarity
Note: Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin By similarity.

GO - Cellular componenti

  1. Prp19 complex Source: UniProtKB
  2. cytoplasm Source: MGI
  3. lipid particle Source: MGI
  4. nuclear speck Source: Ensembl
  5. nucleus Source: MGI
  6. spindle Source: UniProtKB-SubCell
  7. spliceosomal complex Source: MGI
  8. ubiquitin ligase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 504503Pre-mRNA-processing factor 19
PRO_0000051146Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei122 – 1221N6-acetyllysine By similarity
Modified residuei179 – 1791N6-acetyllysine1 Publication
Modified residuei244 – 2441N6-acetyllysine1 Publication
Modified residuei261 – 2611N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ99KP6.
PaxDbiQ99KP6.
PRIDEiQ99KP6.

2D gel databases

REPRODUCTION-2DPAGEQ99KP6.

PTM databases

PhosphoSiteiQ99KP6.

Expressioni

Gene expression databases

ArrayExpressiQ99KP6.
BgeeiQ99KP6.
GenevestigatoriQ99KP6.

Interactioni

Subunit structurei

Homooligomer By similarity. Identified in the spliceosome C complex. Component of the PSO4 complex, composed of PRPF19, CDC5L, PLRG1. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts in the complex directly with CDC5L, PLRG1 and BCAS2. Interacts with APEX1, DNTT and PSMB4 By similarity. Interacts with CWC22 and EIF4A3 in an RNA-independent manner By similarity.

Protein-protein interaction databases

BioGridi205709. 2 interactions.
IntActiQ99KP6. 4 interactions.
MINTiMINT-1867830.

Structurei

3D structure databases

ProteinModelPortaliQ99KP6.
SMRiQ99KP6. Positions 3-55, 195-504.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 7372U-box
Add
BLAST
Repeati219 – 25941WD 1
Add
BLAST
Repeati262 – 30140WD 2
Add
BLAST
Repeati304 – 34542WD 3
Add
BLAST
Repeati348 – 38740WD 4
Add
BLAST
Repeati390 – 42940WD 5
Add
BLAST
Repeati433 – 47240WD 6
Add
BLAST
Repeati473 – 50331WD 7
Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat PRP19 family.
Contains 1 U-box domain.
Contains 7 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00740000115057.
HOVERGENiHBG053697.
InParanoidiQ99KP6.
KOiK10599.
PhylomeDBiQ99KP6.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR013915. Pre-mRNA_splic_Prp19.
IPR000772. Ricin_B_lectin.
IPR003613. Ubox_domain.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF08606. Prp19. 1 hit.
PF04564. U-box. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00504. Ubox. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS51698. U_BOX. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q99KP6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE    50
QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHSF TLRQQLQTTR 100
QELSHALYQH DAACRVIARL TKEVTAAREA LATLKPQAGL IVPQAVPSSQ 150
PSVVGAGEPM DLGELVGMTP EIIQKLQDKA TVLTTERKKR GKTVPEELVK 200
PEELSKYRQV ASHVGLHSAS IPGILALDLC PSDTNKILTG GADKNVVVFD 250
KSTEQILATL KGHTKKVTSV VFHPSQELVF SASPDATIRI WSVPNTSCVQ 300
VVRAHESAVT GLSLHATGDY LLSSSDDQYW AFSDIQTGRV LTKVTDETSG 350
CSLTCAQFHP DGLIFGTGTM DSQIKIWDLK ERTNVANFPG HSGPITSIAF 400
SENGYYLATA ADDSSVKLWD LRKLKNFKTL QLDNNFEVKS LIFDQSGTYL 450
ALGGTDVQIY ICKQWTEILH FTEHSGLTTG VAFGHHAKFI ASTGMDRSLK 500
FYSL 504
Length:504
Mass (Da):55,239
Last modified:June 1, 2001 - v1
Checksum:iB2918188A0651024
GO
Isoform 2 (identifier: Q99KP6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     82-82: W → WGWPNSPALPQSSQWPTLSQ

Note: No experimental confirmation available. Dubious isoform.

Show »
Length:523
Mass (Da):57,302
Checksum:i4867008E9F3D969E
GO
Isoform 3 (identifier: Q99KP6-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-85: Missing.

Note: No experimental confirmation available.

Show »
Length:420
Mass (Da):45,920
Checksum:i9F95F645523FFB19
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2 – 8584Missing in isoform 3.
VSP_012192Add
BLAST
Alternative sequencei82 – 821W → WGWPNSPALPQSSQWPTLSQ in isoform 2.
VSP_011945

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF251503 mRNA. Translation: AAK49039.1.
AF386760 Genomic DNA. Translation: AAM21468.1.
AK047693 mRNA. Translation: BAC33127.1.
AK076998 mRNA. Translation: BAC36557.1.
AK088769 mRNA. Translation: BAC40560.1.
AK164681 mRNA. Translation: BAE37873.1.
BC004070 mRNA. Translation: AAH04070.1.
CCDSiCCDS57135.1. [Q99KP6-2]
RefSeqiNP_001240772.1. NM_001253843.1. [Q99KP6-2]
NP_001240773.1. NM_001253844.1.
NP_598890.1. NM_134129.4. [Q99KP6-1]
UniGeneiMm.491104.

Genome annotation databases

EnsembliENSMUST00000025642; ENSMUSP00000025642; ENSMUSG00000024735. [Q99KP6-2]
ENSMUST00000178868; ENSMUSP00000137435; ENSMUSG00000024735.
GeneIDi28000.
KEGGimmu:28000.
UCSCiuc008grf.2. mouse. [Q99KP6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF251503 mRNA. Translation: AAK49039.1 .
AF386760 Genomic DNA. Translation: AAM21468.1 .
AK047693 mRNA. Translation: BAC33127.1 .
AK076998 mRNA. Translation: BAC36557.1 .
AK088769 mRNA. Translation: BAC40560.1 .
AK164681 mRNA. Translation: BAE37873.1 .
BC004070 mRNA. Translation: AAH04070.1 .
CCDSi CCDS57135.1. [Q99KP6-2 ]
RefSeqi NP_001240772.1. NM_001253843.1. [Q99KP6-2 ]
NP_001240773.1. NM_001253844.1.
NP_598890.1. NM_134129.4. [Q99KP6-1 ]
UniGenei Mm.491104.

3D structure databases

ProteinModelPortali Q99KP6.
SMRi Q99KP6. Positions 3-55, 195-504.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 205709. 2 interactions.
IntActi Q99KP6. 4 interactions.
MINTi MINT-1867830.

PTM databases

PhosphoSitei Q99KP6.

2D gel databases

REPRODUCTION-2DPAGE Q99KP6.

Proteomic databases

MaxQBi Q99KP6.
PaxDbi Q99KP6.
PRIDEi Q99KP6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025642 ; ENSMUSP00000025642 ; ENSMUSG00000024735 . [Q99KP6-2 ]
ENSMUST00000178868 ; ENSMUSP00000137435 ; ENSMUSG00000024735 .
GeneIDi 28000.
KEGGi mmu:28000.
UCSCi uc008grf.2. mouse. [Q99KP6-1 ]

Organism-specific databases

CTDi 27339.
MGIi MGI:106247. Prpf19.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00740000115057.
HOVERGENi HBG053697.
InParanoidi Q99KP6.
KOi K10599.
PhylomeDBi Q99KP6.

Miscellaneous databases

NextBioi 306504.
PROi Q99KP6.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q99KP6.
Bgeei Q99KP6.
Genevestigatori Q99KP6.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProi IPR020472. G-protein_beta_WD-40_rep.
IPR013915. Pre-mRNA_splic_Prp19.
IPR000772. Ricin_B_lectin.
IPR003613. Ubox_domain.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
Pfami PF08606. Prp19. 1 hit.
PF04564. U-box. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view ]
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00504. Ubox. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS51698. U_BOX. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of mSNEV, the mouse homologue of the human nuclear matrix protein NMP200."
    Grillari J., Gross S., Katinger H.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Swiss albino.
  2. Fortschegger K., Grillari J., Katinger H.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    Strain: 129/SvJ.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J and NOD.
    Tissue: Corpus striatum, Stomach, Testis and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II.
    Tissue: Mammary gland.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-179 AND LYS-244, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiPRP19_MOUSE
AccessioniPrimary (citable) accession number: Q99KP6
Secondary accession number(s): Q3TP64, Q8BKZ5, Q8BVQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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