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Q99KP6 (PRP19_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-mRNA-processing factor 19
Alternative name(s):
Nuclear matrix protein 200
PRP19/PSO4 homolog
Senescence evasion factor
Gene names
Name:Prpf19
Synonyms:Prp19, Snev
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing By similarity.

Subunit structure

Homooligomer By similarity. Identified in the spliceosome C complex. Component of the PSO4 complex, composed of PRPF19, CDC5L, PLRG1. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts in the complex directly with CDC5L, PLRG1 and BCAS2. Interacts with APEX1, DNTT and PSMB4 By similarity. Interacts with CWC22 and EIF4A3 in an RNA-independent manner By similarity.

Subcellular location

Nucleus By similarity. Nucleusnucleoplasm By similarity. Cytoplasmcytoskeletonspindle By similarity. Note: Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin By similarity.

Sequence similarities

Belongs to the WD repeat PRP19 family.

Contains 1 U-box domain.

Contains 7 WD repeats.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
mRNA processing
mRNA splicing
   Cellular componentCytoplasm
Cytoskeleton
Nucleus
Spliceosome
   Coding sequence diversityAlternative splicing
   DomainRepeat
WD repeat
   LigandDNA-binding
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

inner cell mass cell proliferation

Inferred from mutant phenotype PubMed 17283042. Source: MGI

lipid biosynthetic process

Inferred from direct assay PubMed 17118936. Source: MGI

mRNA splicing, via spliceosome

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of neuron differentiation

Inferred from direct assay PubMed 16352598. Source: MGI

positive regulation of astrocyte differentiation

Inferred from direct assay PubMed 16352598. Source: MGI

positive regulation of mRNA splicing, via spliceosome

Inferred from mutant phenotype PubMed 18480465. Source: MGI

positive regulation of neuron differentiation

Inferred from sequence alignment PubMed 16352598. Source: MGI

protein polyubiquitination

Inferred from electronic annotation. Source: Ensembl

spliceosomal complex assembly

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentPrp19 complex

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from direct assay PubMed 16352598. Source: MGI

lipid particle

Inferred from direct assay PubMed 17118936. Source: MGI

nuclear speck

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 16352598PubMed 18480465. Source: MGI

spindle

Inferred from electronic annotation. Source: UniProtKB-SubCell

spliceosomal complex

Inferred from sequence alignment PubMed 16352598. Source: MGI

ubiquitin ligase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-ubiquitin ligase activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99KP6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99KP6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     82-82: W → WGWPNSPALPQSSQWPTLSQ
Note: No experimental confirmation available. Dubious isoform.
Isoform 3 (identifier: Q99KP6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     2-85: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 504503Pre-mRNA-processing factor 19
PRO_0000051146

Regions

Domain2 – 7372U-box
Repeat219 – 25941WD 1
Repeat262 – 30140WD 2
Repeat304 – 34542WD 3
Repeat348 – 38740WD 4
Repeat390 – 42940WD 5
Repeat433 – 47240WD 6
Repeat473 – 50331WD 7

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue1221N6-acetyllysine By similarity
Modified residue1791N6-acetyllysine Ref.5
Modified residue2441N6-acetyllysine Ref.5
Modified residue2611N6-acetyllysine By similarity

Natural variations

Alternative sequence2 – 8584Missing in isoform 3.
VSP_012192
Alternative sequence821W → WGWPNSPALPQSSQWPTLSQ in isoform 2.
VSP_011945

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: B2918188A0651024

FASTA50455,239
        10         20         30         40         50         60 
MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP 

        70         80         90        100        110        120 
IRPKPPSATS IPAILKALQD EWDAVMLHSF TLRQQLQTTR QELSHALYQH DAACRVIARL 

       130        140        150        160        170        180 
TKEVTAAREA LATLKPQAGL IVPQAVPSSQ PSVVGAGEPM DLGELVGMTP EIIQKLQDKA 

       190        200        210        220        230        240 
TVLTTERKKR GKTVPEELVK PEELSKYRQV ASHVGLHSAS IPGILALDLC PSDTNKILTG 

       250        260        270        280        290        300 
GADKNVVVFD KSTEQILATL KGHTKKVTSV VFHPSQELVF SASPDATIRI WSVPNTSCVQ 

       310        320        330        340        350        360 
VVRAHESAVT GLSLHATGDY LLSSSDDQYW AFSDIQTGRV LTKVTDETSG CSLTCAQFHP 

       370        380        390        400        410        420 
DGLIFGTGTM DSQIKIWDLK ERTNVANFPG HSGPITSIAF SENGYYLATA ADDSSVKLWD 

       430        440        450        460        470        480 
LRKLKNFKTL QLDNNFEVKS LIFDQSGTYL ALGGTDVQIY ICKQWTEILH FTEHSGLTTG 

       490        500 
VAFGHHAKFI ASTGMDRSLK FYSL 

« Hide

Isoform 2 [UniParc].

Checksum: 4867008E9F3D969E
Show »

FASTA52357,302
Isoform 3 [UniParc].

Checksum: 9F95F645523FFB19
Show »

FASTA42045,920

References

« Hide 'large scale' references
[1]"Cloning of mSNEV, the mouse homologue of the human nuclear matrix protein NMP200."
Grillari J., Gross S., Katinger H.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: Swiss albino.
[2]Fortschegger K., Grillari J., Katinger H.
Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
Strain: 129/SvJ.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Strain: C57BL/6J and NOD.
Tissue: Corpus striatum, Stomach, Testis and Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Czech II.
Tissue: Mammary gland.
[5]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-179 AND LYS-244, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF251503 mRNA. Translation: AAK49039.1.
AF386760 Genomic DNA. Translation: AAM21468.1.
AK047693 mRNA. Translation: BAC33127.1.
AK076998 mRNA. Translation: BAC36557.1.
AK088769 mRNA. Translation: BAC40560.1.
AK164681 mRNA. Translation: BAE37873.1.
BC004070 mRNA. Translation: AAH04070.1.
RefSeqNP_001240772.1. NM_001253843.1.
NP_001240773.1. NM_001253844.1.
NP_598890.1. NM_134129.4.
UniGeneMm.491104.

3D structure databases

ProteinModelPortalQ99KP6.
SMRQ99KP6. Positions 3-55, 195-504.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid205709. 1 interaction.
IntActQ99KP6. 4 interactions.
MINTMINT-1867830.

PTM databases

PhosphoSiteQ99KP6.

2D gel databases

REPRODUCTION-2DPAGEQ99KP6.

Proteomic databases

PaxDbQ99KP6.
PRIDEQ99KP6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025642; ENSMUSP00000025642; ENSMUSG00000024735. [Q99KP6-2]
ENSMUST00000178868; ENSMUSP00000137435; ENSMUSG00000024735.
GeneID28000.
KEGGmmu:28000.
UCSCuc008grf.2. mouse. [Q99KP6-1]

Organism-specific databases

CTD27339.
MGIMGI:106247. Prpf19.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00740000115057.
HOVERGENHBG053697.
InParanoidQ99KP6.
KOK10599.
PhylomeDBQ99KP6.

Gene expression databases

ArrayExpressQ99KP6.
BgeeQ99KP6.
GenevestigatorQ99KP6.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR013915. Pre-mRNA_splic_Prp19.
IPR000772. Ricin_B_lectin.
IPR003613. Ubox_domain.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF08606. Prp19. 1 hit.
PF04564. U-box. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00504. Ubox. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS51698. U_BOX. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio306504.
PROQ99KP6.
SOURCESearch...

Entry information

Entry namePRP19_MOUSE
AccessionPrimary (citable) accession number: Q99KP6
Secondary accession number(s): Q3TP64, Q8BKZ5, Q8BVQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot