Q99KP6 (PRP19_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-processing factor 19

Alternative name(s):
Nuclear matrix protein 200
PRP19/PSO4 homolog
Senescence evasion factor

Gene names

Name:	Prpf19
Synonyms:	Prp19, Snev

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing By similarity.
Subunit structure	Homooligomer By similarity. Identified in the spliceosome C complex. Component of the PSO4 complex, composed of PRPF19, CDC5L, PLRG1. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts in the complex directly with CDC5L, PLRG1 and BCAS2. Interacts with APEX1, DNTT and PSMB4 By similarity.
Subcellular location	Nucleus By similarity. Nucleus › nucleoplasm By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Note: Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin By similarity.
Sequence similarities	Belongs to the WD repeat PRP19 family. Contains 1 U-box domain. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	DNA damage DNA repair mRNA processing mRNA splicing
Cellular component	Cytoplasm Cytoskeleton Nucleus Spliceosome
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat
Ligand	DNA-binding
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW inner cell mass cell proliferation Inferred from mutant phenotype PubMed 17283042. Source: MGI lipid biosynthetic process Inferred from direct assay PubMed 17118936. Source: MGI mRNA splicing, via spliceosome Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of neuron differentiation Inferred from direct assay PubMed 16352598. Source: MGI positive regulation of astrocyte differentiation Inferred from direct assay PubMed 16352598. Source: MGI positive regulation of mRNA splicing, via spliceosome Inferred from mutant phenotype PubMed 18480465. Source: MGI positive regulation of neuron differentiation Inferred from sequence alignment PubMed 16352598. Source: MGI protein polyubiquitination Inferred from electronic annotation. Source: Compara spliceosomal complex assembly Inferred from electronic annotation. Source: Compara
Cellular_component	Prp19 complex Inferred from sequence or structural similarity. Source: UniProtKB lipid particle Inferred from direct assay PubMed 17118936. Source: MGI nuclear speck Inferred from electronic annotation. Source: Compara spindle Inferred from electronic annotation. Source: UniProtKB-SubCell spliceosomal complex Inferred from sequence alignment PubMed 16352598. Source: MGI ubiquitin ligase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-ubiquitin ligase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99KP6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99KP6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     82-82: W → WGWPNSPALPQSSQWPTLSQ

Note: No experimental confirmation available.

Isoform 3 (identifier: Q99KP6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 504	503	Pre-mRNA-processing factor 19		PRO_0000051146
Regions
	Domain	2 – 68	67	U-box
	Repeat	219 – 259	41	WD 1
	Repeat	262 – 301	40	WD 2
	Repeat	304 – 345	42	WD 3
	Repeat	348 – 387	40	WD 4
	Repeat	390 – 429	40	WD 5
	Repeat	433 – 472	40	WD 6
	Repeat	473 – 503	31	WD 7
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	122	1	N6-acetyllysine By similarity
	Modified residue	261	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	1 – 85	85	Missing in isoform 3.		VSP_012192
	Alternative sequence	82	1	W → WGWPNSPALPQSSQWPTLSQ in isoform 2.		VSP_011945

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: B2918188A0651024	FASTA	504	55,239
	10 20 30 40 50 60 MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP 70 80 90 100 110 120 IRPKPPSATS IPAILKALQD EWDAVMLHSF TLRQQLQTTR QELSHALYQH DAACRVIARL 130 140 150 160 170 180 TKEVTAAREA LATLKPQAGL IVPQAVPSSQ PSVVGAGEPM DLGELVGMTP EIIQKLQDKA 190 200 210 220 230 240 TVLTTERKKR GKTVPEELVK PEELSKYRQV ASHVGLHSAS IPGILALDLC PSDTNKILTG 250 260 270 280 290 300 GADKNVVVFD KSTEQILATL KGHTKKVTSV VFHPSQELVF SASPDATIRI WSVPNTSCVQ 310 320 330 340 350 360 VVRAHESAVT GLSLHATGDY LLSSSDDQYW AFSDIQTGRV LTKVTDETSG CSLTCAQFHP 370 380 390 400 410 420 DGLIFGTGTM DSQIKIWDLK ERTNVANFPG HSGPITSIAF SENGYYLATA ADDSSVKLWD 430 440 450 460 470 480 LRKLKNFKTL QLDNNFEVKS LIFDQSGTYL ALGGTDVQIY ICKQWTEILH FTEHSGLTTG 490 500 VAFGHHAKFI ASTGMDRSLK FYSL
	Isoform 2 [UniParc]. Checksum: 4867008E9F3D969E	FASTA	523	57,302
	10 20 30 40 50 60 MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP 70 80 90 100 110 120 IRPKPPSATS IPAILKALQD EWGWPNSPAL PQSSQWPTLS QDAVMLHSFT LRQQLQTTRQ 130 140 150 160 170 180 ELSHALYQHD AACRVIARLT KEVTAAREAL ATLKPQAGLI VPQAVPSSQP SVVGAGEPMD 190 200 210 220 230 240 LGELVGMTPE IIQKLQDKAT VLTTERKKRG KTVPEELVKP EELSKYRQVA SHVGLHSASI 250 260 270 280 290 300 PGILALDLCP SDTNKILTGG ADKNVVVFDK STEQILATLK GHTKKVTSVV FHPSQELVFS 310 320 330 340 350 360 ASPDATIRIW SVPNTSCVQV VRAHESAVTG LSLHATGDYL LSSSDDQYWA FSDIQTGRVL 370 380 390 400 410 420 TKVTDETSGC SLTCAQFHPD GLIFGTGTMD SQIKIWDLKE RTNVANFPGH SGPITSIAFS 430 440 450 460 470 480 ENGYYLATAA DDSSVKLWDL RKLKNFKTLQ LDNNFEVKSL IFDQSGTYLA LGGTDVQIYI 490 500 510 520 CKQWTEILHF TEHSGLTTGV AFGHHAKFIA STGMDRSLKF YSL
	Isoform 3 [UniParc]. Checksum: 56AD02B0FBFB1B48	FASTA	419	45,789
	10 20 30 40 50 60 MLHSFTLRQQ LQTTRQELSH ALYQHDAACR VIARLTKEVT AAREALATLK PQAGLIVPQA 70 80 90 100 110 120 VPSSQPSVVG AGEPMDLGEL VGMTPEIIQK LQDKATVLTT ERKKRGKTVP EELVKPEELS 130 140 150 160 170 180 KYRQVASHVG LHSASIPGIL ALDLCPSDTN KILTGGADKN VVVFDKSTEQ ILATLKGHTK 190 200 210 220 230 240 KVTSVVFHPS QELVFSASPD ATIRIWSVPN TSCVQVVRAH ESAVTGLSLH ATGDYLLSSS 250 260 270 280 290 300 DDQYWAFSDI QTGRVLTKVT DETSGCSLTC AQFHPDGLIF GTGTMDSQIK IWDLKERTNV 310 320 330 340 350 360 ANFPGHSGPI TSIAFSENGY YLATAADDSS VKLWDLRKLK NFKTLQLDNN FEVKSLIFDQ 370 380 390 400 410 SGTYLALGGT DVQIYICKQW TEILHFTEHS GLTTGVAFGH HAKFIASTGM DRSLKFYSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of mSNEV, the mouse homologue of the human nuclear matrix protein NMP200." Grillari J., Gross S., Katinger H. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: Swiss albino.
[2]	Fortschegger K., Grillari J., Katinger H. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1). Strain: 129/SvJ.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6J and NOD. Tissue: Corpus striatum, Stomach, Testis and Thymus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Czech II. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF251503 mRNA. Translation: AAK49039.1. AF386760 Genomic DNA. Translation: AAM21468.1. AK047693 mRNA. Translation: BAC33127.1. AK076998 mRNA. Translation: BAC36557.1. AK088769 mRNA. Translation: BAC40560.1. AK164681 mRNA. Translation: BAE37873.1. BC004070 mRNA. Translation: AAH04070.1.
IPI	IPI00222760. IPI00480440. IPI00480507.
RefSeq	NP_001240772.1. NM_001253843.1. NP_001240773.1. NM_001253844.1. NP_598890.1. NM_134129.4.
UniGene	Mm.225898.
3D structure databases
ProteinModelPortal	Q99KP6.
SMR	Q99KP6. Positions 3-55, 202-503.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99KP6. 1 interaction.
PTM databases
PhosphoSite	Q99KP6.
2D gel databases
REPRODUCTION-2DPAGE	Q99KP6.
Proteomic databases
PaxDb	Q99KP6.
PRIDE	Q99KP6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025642; ENSMUSP00000025642; ENSMUSG00000024735. ENSMUST00000178868; ENSMUSP00000137435; ENSMUSG00000024735.
GeneID	28000.
KEGG	mmu:28000.
UCSC	uc008grf.1. mouse.
Organism-specific databases
CTD	27339.
MGI	MGI:106247. Prpf19.
Phylogenomic databases
eggNOG	COG2319.
GeneTree	ENSGT00700000104487.
HOVERGEN	HBG053697.
InParanoid	Q99KP6.
KO	K10599.
OrthoDB	EOG4V6ZGG.
Gene expression databases
ArrayExpress	Q99KP6.
Bgee	Q99KP6.
Genevestigator	Q99KP6.
GermOnline	ENSMUSG00000024735. Mus musculus.
Family and domain databases
Gene3D	2.130.10.10. 1 hit. 3.30.40.10. 1 hit.
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR013915. Pre-mRNA_splic_Prp19. IPR000772. Ricin_B_lectin. IPR003613. Ubox_domain. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF08606. Prp19. 1 hit. PF04564. U-box. 1 hit. PF00400. WD40. 5 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00504. Ubox. 1 hit. SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 4 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	306504.
SOURCE	Search...

Entry information

Entry name

PRP19_MOUSE

Accession

Primary (citable) accession number: Q99KP6
Secondary accession number(s): Q3TP64, Q8BKZ5, Q8BVQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program