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Protein

Clathrin interactor 1

Gene

Clint1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371Phosphatidylinositol lipid headgroupBy similarity
Binding sitei75 – 751Phosphatidylinositol lipid headgroupBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin interactor 1
Alternative name(s):
Enthoprotin
Epsin-4
Epsin-related protein
Short name:
EpsinR
Gene namesi
Name:Clint1
Synonyms:Enth, Epn4, Epnr, Kiaa0171
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2144243. Clint1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 631631Clathrin interactor 1PRO_0000074522Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei171 – 1711PhosphoserineCombined sources
Modified residuei174 – 1741PhosphoserineBy similarity
Modified residuei213 – 2131PhosphoserineCombined sources
Modified residuei218 – 2181PhosphoserineBy similarity
Modified residuei235 – 2351PhosphoserineBy similarity
Modified residuei253 – 2531PhosphoserineBy similarity
Modified residuei307 – 3071PhosphoserineBy similarity
Modified residuei316 – 3161PhosphothreonineBy similarity
Modified residuei320 – 3201PhosphoserineBy similarity
Modified residuei630 – 6301PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99KN9.
MaxQBiQ99KN9.
PaxDbiQ99KN9.
PeptideAtlasiQ99KN9.
PRIDEiQ99KN9.

PTM databases

iPTMnetiQ99KN9.
PhosphoSiteiQ99KN9.

Expressioni

Gene expression databases

CleanExiMM_CLINT1.

Interactioni

Subunit structurei

Binds clathrin heavy chain, GGA2, AP-2 and AP1G1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ99KN9. 5 interactions.
MINTiMINT-1870281.
STRINGi10090.ENSMUSP00000104883.

Structurei

3D structure databases

ProteinModelPortaliQ99KN9.
SMRiQ99KN9. Positions 25-160.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 157134ENTHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi555 – 61157Met-richAdd
BLAST

Sequence similaritiesi

Belongs to the epsin family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2056. Eukaryota.
ENOG410XSM0. LUCA.
HOGENOMiHOG000082415.
HOVERGENiHBG048921.
InParanoidiQ99KN9.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR030544. CLINT1.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PANTHERiPTHR12276:SF53. PTHR12276:SF53. 1 hit.
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99KN9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLIFMYLYVC VCTCTCAFSL CSTNVVMNYS EIESKVREAT NDDPWGPSGQ
60 70 80 90 100
LMGEIAKATF MYEQFPELMN MLWSRMLKDN KKNWRRVYKS LLLLAYLIRN
110 120 130 140 150
GSERVVTSAR EHIYDLRSLE NYHFVDEHGK DQGINIRQKV KELVEFAQDD
160 170 180 190 200
DRLREERKKA KKNKDKYVGV SSDSVGGFRY NERYDPEPKS KWDEEWDKNK
210 220 230 240 250
SAFPFSDKLG ELSDKIGSTI DDTISKFRRK DREDSPERCS DSDEEKKARR
260 270 280 290 300
GRSPKGEFKD EEETVTTKHI HITQATETTT TRHKRTANPS KTIDLGAAAH
310 320 330 340 350
YTGDKASPDQ NASTHTPQSS AKPSVPSSKS SGDLVDLFDG SSQSAGGSAD
360 370 380 390 400
LFGGFADFGS AAASGNFPSQ ATSGNGDFGD WSAFNQAPSG PVASGGELFG
410 420 430 440 450
SAPQSAVELI SASQPALGPP PAASNSADLF DLMGSSQATM TSSQSMNFSL
460 470 480 490 500
MSTNTVGLGL PMSRSQNTDM VQKSASKTLP STWSDPSVNI SLDNLLPGMQ
510 520 530 540 550
PSKPQQPSLN TMIQQQNMQQ PLNVMTQSFG AVNLSSPSNM LPVRPQTNPL
560 570 580 590 600
LGGPMPMNMP GVMTGTMGMA PLGNSAGMSQ GMVGMNMNMG MSASGMGLSG
610 620 630
TMGMGMPSMA MPSGTVQPKQ DAFANFANFS K
Length:631
Mass (Da):68,513
Last modified:January 16, 2004 - v2
Checksum:i370711BBCA59CC17
GO
Isoform 2 (identifier: Q99KN9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     346-370: Missing.
     467-489: NTDMVQKSASKTLPSTWSDPSVN → VSCLFPLGIGAYTSTRRSNSMMS
     490-631: Missing.

Note: No experimental confirmation available.
Show »
Length:464
Mass (Da):51,425
Checksum:i6A1DEF23CEF19373
GO

Sequence cautioni

The sequence BAC41396.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei346 – 37025Missing in isoform 2. 1 PublicationVSP_009162Add
BLAST
Alternative sequencei467 – 48923NTDMV…DPSVN → VSCLFPLGIGAYTSTRRSNS MMS in isoform 2. 1 PublicationVSP_009163Add
BLAST
Alternative sequencei490 – 631142Missing in isoform 2. 1 PublicationVSP_009164Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093212 mRNA. Translation: BAC41396.1. Different initiation.
BC004080 mRNA. Translation: AAH04080.1.
UniGeneiMm.169673.
Mm.475268.

Genome annotation databases

UCSCiuc007inr.1. mouse. [Q99KN9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093212 mRNA. Translation: BAC41396.1. Different initiation.
BC004080 mRNA. Translation: AAH04080.1.
UniGeneiMm.169673.
Mm.475268.

3D structure databases

ProteinModelPortaliQ99KN9.
SMRiQ99KN9. Positions 25-160.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99KN9. 5 interactions.
MINTiMINT-1870281.
STRINGi10090.ENSMUSP00000104883.

PTM databases

iPTMnetiQ99KN9.
PhosphoSiteiQ99KN9.

Proteomic databases

EPDiQ99KN9.
MaxQBiQ99KN9.
PaxDbiQ99KN9.
PeptideAtlasiQ99KN9.
PRIDEiQ99KN9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc007inr.1. mouse. [Q99KN9-2]

Organism-specific databases

MGIiMGI:2144243. Clint1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2056. Eukaryota.
ENOG410XSM0. LUCA.
HOGENOMiHOG000082415.
HOVERGENiHBG048921.
InParanoidiQ99KN9.

Miscellaneous databases

ChiTaRSiClint1. mouse.
PROiQ99KN9.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CLINT1.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR030544. CLINT1.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PANTHERiPTHR12276:SF53. PTHR12276:SF53. 1 hit.
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
    Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Embryonic intestine.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 101-631 (ISOFORM 1).
    Tissue: Mammary tumor.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiEPN4_MOUSE
AccessioniPrimary (citable) accession number: Q99KN9
Secondary accession number(s): Q8CFH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: July 6, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.