Q99KN9 (EPN4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Clathrin interactor 1

Alternative name(s):
Enthoprotin
Epsin-4
Epsin-related protein
Short name=EpsinR

Gene names

Name:	Clint1
Synonyms:	Enth, Epn4, Epnr, Kiaa0171

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly By similarity.
Subunit structure	Binds clathrin heavy chain, GGA2, AP-2 and AP1G1 By similarity.
Subcellular location	Cytoplasm By similarity. Cytoplasm › perinuclear region By similarity. Membrane; Peripheral membrane protein By similarity. Cytoplasmic vesicle › clathrin-coated vesicle By similarity. Note: Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network By similarity.
Sequence similarities	Belongs to the epsin family. Contains 1 ENTH (epsin N-terminal homology) domain.
Sequence caution	The sequence BAC41396.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	clathrin-coated vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99KN9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99KN9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     346-370: Missing.
     467-489: NTDMVQKSASKTLPSTWSDPSVN → VSCLFPLGIGAYTSTRRSNSMMS
     490-631: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 631	631	Clathrin interactor 1		PRO_0000074522
Regions
	Domain	24 – 157	134	ENTH
	Compositional bias	555 – 611	57	Met-rich
Sites
	Binding site	37	1	Phosphatidylinositol lipid headgroup By similarity
	Binding site	75	1	Phosphatidylinositol lipid headgroup By similarity
Amino acid modifications
	Modified residue	171	1	Phosphoserine Ref.3
	Modified residue	235	1	Phosphoserine Ref.4
	Modified residue	240	1	Phosphoserine Ref.4
	Modified residue	242	1	Phosphoserine Ref.4
	Modified residue	253	1	Phosphoserine By similarity
	Modified residue	307	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	346 – 370	25	Missing in isoform 2.		VSP_009162
	Alternative sequence	467 – 489	23	NTDMV…DPSVN → VSCLFPLGIGAYTSTRRSNS MMS in isoform 2.		VSP_009163
	Alternative sequence	490 – 631	142	Missing in isoform 2.		VSP_009164

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: 370711BBCA59CC17	FASTA	631	68,513
	10 20 30 40 50 60 MLIFMYLYVC VCTCTCAFSL CSTNVVMNYS EIESKVREAT NDDPWGPSGQ LMGEIAKATF 70 80 90 100 110 120 MYEQFPELMN MLWSRMLKDN KKNWRRVYKS LLLLAYLIRN GSERVVTSAR EHIYDLRSLE 130 140 150 160 170 180 NYHFVDEHGK DQGINIRQKV KELVEFAQDD DRLREERKKA KKNKDKYVGV SSDSVGGFRY 190 200 210 220 230 240 NERYDPEPKS KWDEEWDKNK SAFPFSDKLG ELSDKIGSTI DDTISKFRRK DREDSPERCS 250 260 270 280 290 300 DSDEEKKARR GRSPKGEFKD EEETVTTKHI HITQATETTT TRHKRTANPS KTIDLGAAAH 310 320 330 340 350 360 YTGDKASPDQ NASTHTPQSS AKPSVPSSKS SGDLVDLFDG SSQSAGGSAD LFGGFADFGS 370 380 390 400 410 420 AAASGNFPSQ ATSGNGDFGD WSAFNQAPSG PVASGGELFG SAPQSAVELI SASQPALGPP 430 440 450 460 470 480 PAASNSADLF DLMGSSQATM TSSQSMNFSL MSTNTVGLGL PMSRSQNTDM VQKSASKTLP 490 500 510 520 530 540 STWSDPSVNI SLDNLLPGMQ PSKPQQPSLN TMIQQQNMQQ PLNVMTQSFG AVNLSSPSNM 550 560 570 580 590 600 LPVRPQTNPL LGGPMPMNMP GVMTGTMGMA PLGNSAGMSQ GMVGMNMNMG MSASGMGLSG 610 620 630 TMGMGMPSMA MPSGTVQPKQ DAFANFANFS K
	Isoform 2 [UniParc]. Checksum: 6A1DEF23CEF19373	FASTA	464	51,425
	10 20 30 40 50 60 MLIFMYLYVC VCTCTCAFSL CSTNVVMNYS EIESKVREAT NDDPWGPSGQ LMGEIAKATF 70 80 90 100 110 120 MYEQFPELMN MLWSRMLKDN KKNWRRVYKS LLLLAYLIRN GSERVVTSAR EHIYDLRSLE 130 140 150 160 170 180 NYHFVDEHGK DQGINIRQKV KELVEFAQDD DRLREERKKA KKNKDKYVGV SSDSVGGFRY 190 200 210 220 230 240 NERYDPEPKS KWDEEWDKNK SAFPFSDKLG ELSDKIGSTI DDTISKFRRK DREDSPERCS 250 260 270 280 290 300 DSDEEKKARR GRSPKGEFKD EEETVTTKHI HITQATETTT TRHKRTANPS KTIDLGAAAH 310 320 330 340 350 360 YTGDKASPDQ NASTHTPQSS AKPSVPSSKS SGDLVDLFDG SSQSAATSGN GDFGDWSAFN 370 380 390 400 410 420 QAPSGPVASG GELFGSAPQS AVELISASQP ALGPPPAASN SADLFDLMGS SQATMTSSQS 430 440 450 460 MNFSLMSTNT VGLGLPMSRS QVSCLFPLGI GAYTSTRRSN SMMS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Embryonic intestine.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 101-631 (ISOFORM 1). Tissue: Mammary tumor.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171, MASS SPECTROMETRY. Tissue: Liver.
[4]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-235; SER-240; SER-242 AND SER-307, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB093212 mRNA. Translation: BAC41396.1. Different initiation. BC004080 mRNA. Translation: AAH04080.1.
IPI	IPI00400154. IPI00403139.
UniGene	Mm.169673.
3D structure databases
ProteinModelPortal	Q99KN9.
SMR	Q99KN9. Positions 25-160.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1870281.
STRING	10090.ENSMUSP00000104884.
PTM databases
PhosphoSite	Q99KN9.
Proteomic databases
PaxDb	Q99KN9.
PRIDE	Q99KN9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	uc007inr.1. mouse.
Organism-specific databases
MGI	MGI:2144243. Clint1.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG290795.
HOGENOM	HOG000082415.
HOVERGEN	HBG048921.
Gene expression databases
CleanEx	MM_CLINT1.
Genevestigator	Q99KN9.
GermOnline	ENSMUSG00000006169. Mus musculus.
Family and domain databases
Gene3D	1.25.40.90. 1 hit.
InterPro	IPR008942. ENTH_VHS. IPR013809. Epsin-like_N. IPR001026. Epsin_dom_N. [Graphical view]
Pfam	PF01417. ENTH. 1 hit. [Graphical view]
SMART	SM00273. ENTH. 1 hit. [Graphical view]
SUPFAM	SSF48464. ENTH_VHS. 1 hit.
PROSITE	PS50942. ENTH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CLINT1. mouse.
SOURCE	Search...

Entry information

Entry name

EPN4_MOUSE

Accession

Primary (citable) accession number: Q99KN9
Secondary accession number(s): Q8CFH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	May 29, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program