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Protein

Clathrin interactor 1

Gene

Clint1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei75Phosphatidylinositol lipid headgroupBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin interactor 1
Alternative name(s):
Enthoprotin
Epsin-4
Epsin-related protein
Short name:
EpsinR
Gene namesi
Name:Clint1
Synonyms:Enth, Epn4, Epnr, Kiaa0171
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2144243. Clint1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745221 – 631Clathrin interactor 1Add BLAST631

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei171PhosphoserineCombined sources1
Modified residuei174PhosphoserineBy similarity1
Modified residuei213PhosphoserineCombined sources1
Modified residuei218PhosphoserineBy similarity1
Modified residuei235PhosphoserineBy similarity1
Modified residuei253PhosphoserineBy similarity1
Modified residuei307PhosphoserineBy similarity1
Modified residuei316PhosphothreonineBy similarity1
Modified residuei320PhosphoserineBy similarity1
Modified residuei630PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99KN9.
PaxDbiQ99KN9.
PeptideAtlasiQ99KN9.
PRIDEiQ99KN9.

PTM databases

iPTMnetiQ99KN9.
PhosphoSitePlusiQ99KN9.

Expressioni

Gene expression databases

CleanExiMM_CLINT1.

Interactioni

Subunit structurei

Binds clathrin heavy chain, GGA2, AP-2 and AP1G1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ99KN9. 5 interactors.
MINTiMINT-1870281.
STRINGi10090.ENSMUSP00000104883.

Structurei

3D structure databases

ProteinModelPortaliQ99KN9.
SMRiQ99KN9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 157ENTHPROSITE-ProRule annotationAdd BLAST134

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi555 – 611Met-richAdd BLAST57

Sequence similaritiesi

Belongs to the epsin family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2056. Eukaryota.
ENOG410XSM0. LUCA.
HOGENOMiHOG000082415.
HOVERGENiHBG048921.
InParanoidiQ99KN9.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR030544. CLINT1.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PANTHERiPTHR12276:SF53. PTHR12276:SF53. 1 hit.
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99KN9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLIFMYLYVC VCTCTCAFSL CSTNVVMNYS EIESKVREAT NDDPWGPSGQ
60 70 80 90 100
LMGEIAKATF MYEQFPELMN MLWSRMLKDN KKNWRRVYKS LLLLAYLIRN
110 120 130 140 150
GSERVVTSAR EHIYDLRSLE NYHFVDEHGK DQGINIRQKV KELVEFAQDD
160 170 180 190 200
DRLREERKKA KKNKDKYVGV SSDSVGGFRY NERYDPEPKS KWDEEWDKNK
210 220 230 240 250
SAFPFSDKLG ELSDKIGSTI DDTISKFRRK DREDSPERCS DSDEEKKARR
260 270 280 290 300
GRSPKGEFKD EEETVTTKHI HITQATETTT TRHKRTANPS KTIDLGAAAH
310 320 330 340 350
YTGDKASPDQ NASTHTPQSS AKPSVPSSKS SGDLVDLFDG SSQSAGGSAD
360 370 380 390 400
LFGGFADFGS AAASGNFPSQ ATSGNGDFGD WSAFNQAPSG PVASGGELFG
410 420 430 440 450
SAPQSAVELI SASQPALGPP PAASNSADLF DLMGSSQATM TSSQSMNFSL
460 470 480 490 500
MSTNTVGLGL PMSRSQNTDM VQKSASKTLP STWSDPSVNI SLDNLLPGMQ
510 520 530 540 550
PSKPQQPSLN TMIQQQNMQQ PLNVMTQSFG AVNLSSPSNM LPVRPQTNPL
560 570 580 590 600
LGGPMPMNMP GVMTGTMGMA PLGNSAGMSQ GMVGMNMNMG MSASGMGLSG
610 620 630
TMGMGMPSMA MPSGTVQPKQ DAFANFANFS K
Length:631
Mass (Da):68,513
Last modified:January 16, 2004 - v2
Checksum:i370711BBCA59CC17
GO
Isoform 2 (identifier: Q99KN9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     346-370: Missing.
     467-489: NTDMVQKSASKTLPSTWSDPSVN → VSCLFPLGIGAYTSTRRSNSMMS
     490-631: Missing.

Note: No experimental confirmation available.
Show »
Length:464
Mass (Da):51,425
Checksum:i6A1DEF23CEF19373
GO

Sequence cautioni

The sequence BAC41396 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009162346 – 370Missing in isoform 2. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_009163467 – 489NTDMV…DPSVN → VSCLFPLGIGAYTSTRRSNS MMS in isoform 2. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_009164490 – 631Missing in isoform 2. 1 PublicationAdd BLAST142

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093212 mRNA. Translation: BAC41396.1. Different initiation.
BC004080 mRNA. Translation: AAH04080.1.
UniGeneiMm.169673.
Mm.475268.

Genome annotation databases

UCSCiuc007inr.1. mouse. [Q99KN9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093212 mRNA. Translation: BAC41396.1. Different initiation.
BC004080 mRNA. Translation: AAH04080.1.
UniGeneiMm.169673.
Mm.475268.

3D structure databases

ProteinModelPortaliQ99KN9.
SMRiQ99KN9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99KN9. 5 interactors.
MINTiMINT-1870281.
STRINGi10090.ENSMUSP00000104883.

PTM databases

iPTMnetiQ99KN9.
PhosphoSitePlusiQ99KN9.

Proteomic databases

EPDiQ99KN9.
PaxDbiQ99KN9.
PeptideAtlasiQ99KN9.
PRIDEiQ99KN9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc007inr.1. mouse. [Q99KN9-2]

Organism-specific databases

MGIiMGI:2144243. Clint1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2056. Eukaryota.
ENOG410XSM0. LUCA.
HOGENOMiHOG000082415.
HOVERGENiHBG048921.
InParanoidiQ99KN9.

Miscellaneous databases

ChiTaRSiClint1. mouse.
PROiQ99KN9.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CLINT1.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR030544. CLINT1.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PANTHERiPTHR12276:SF53. PTHR12276:SF53. 1 hit.
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEPN4_MOUSE
AccessioniPrimary (citable) accession number: Q99KN9
Secondary accession number(s): Q8CFH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: November 2, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.