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Protein

Probable histidine--tRNA ligase, mitochondrial

Gene

Hars2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei157L-histidineBy similarity1
Binding sitei173L-histidineBy similarity1
Binding sitei177L-histidineBy similarity1
Binding sitei326L-histidineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable histidine--tRNA ligase, mitochondrial (EC:6.1.1.21)
Alternative name(s):
Histidine--tRNA ligase-like
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:Hars2
Synonyms:Harsl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1918041. Hars2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 31MitochondrionSequence analysisAdd BLAST31
ChainiPRO_000025458432 – 505Probable histidine--tRNA ligase, mitochondrialAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66PhosphoserineCombined sources1
Modified residuei443N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99KK9.
MaxQBiQ99KK9.
PaxDbiQ99KK9.
PeptideAtlasiQ99KK9.
PRIDEiQ99KK9.

PTM databases

iPTMnetiQ99KK9.
PhosphoSitePlusiQ99KK9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000019143.
ExpressionAtlasiQ99KK9. baseline and differential.
GenevisibleiQ99KK9. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000117231.

Structurei

3D structure databases

ProteinModelPortaliQ99KK9.
SMRiQ99KK9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni130 – 132L-histidine bindingBy similarity3
Regioni330 – 331L-histidine bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1936. Eukaryota.
COG0124. LUCA.
GeneTreeiENSGT00390000005922.
HOGENOMiHOG000018075.
HOVERGENiHBG002731.
InParanoidiQ99KK9.
KOiK01892.
OMAiARYVVMH.
OrthoDBiEOG091G05P3.
TreeFamiTF300652.

Family and domain databases

CDDicd00859. HisRS_anticodon. 1 hit.
Gene3Di3.40.50.800. 1 hit.
InterProiView protein in InterPro
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
IPR033656. HisRS_anticodon.
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiView protein in Pfam
PF03129. HGTP_anticodon. 1 hit.
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiView protein in PROSITE
PS50862. AA_TRNA_LIGASE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99KK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHLGPLRRR AWAALLGQLL RPPSTVCTRG CHSQVAKAVL TSEQLKSHQE
60 70 80 90 100
KPNFVIKVPK GTRDLSPQQM VVREKILDKI ISCFKRHGAK GLDTPAFELK
110 120 130 140 150
EMLTEKYEDN FGLMYDLKDQ GGELLSLRYD LTVPFARYLA MNKLKKMKRY
160 170 180 190 200
QVGKVWRRES PAIAQGRYRE FCQCDFDIAG EFDPMIPDAE CLRIMCEILS
210 220 230 240 250
GLQLGDFLIK VNDRRVVDGI FAVCGVPESK LRTICSSMDK LDKMSWEGVR
260 270 280 290 300
HEMVAKKGLA PEVADRIGDF VQYHGGISLV EDLFKDPRLS QSQLALQGLG
310 320 330 340 350
DLKLLFEYLR LFGIADKISL DLSLARGLDY YTGVIYEAVL LESPAQAGKE
360 370 380 390 400
TLSVGSVAAG GRYDNLVAQF DPKGHHVPCV GLSIGVERIF YLVEQKMKMS
410 420 430 440 450
GEKVRTTETQ VFVATPQKNF LQERLKIIAE LWDAGIKAEM LYKNNPKLLT
460 470 480 490 500
QLHYCEKADI PLMVIIGEQE RNEGVIKLRS VASREEVTIN RESLVAEIQK

RLSES
Length:505
Mass (Da):56,985
Last modified:June 1, 2001 - v1
Checksum:i1865426DF408DA1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC004596 mRNA. Translation: AAH04596.1.
CCDSiCCDS29165.1.
RefSeqiNP_542367.1. NM_080636.2.
UniGeneiMm.282700.

Genome annotation databases

EnsembliENSMUST00000152954; ENSMUSP00000117231; ENSMUSG00000019143.
GeneIDi70791.
KEGGimmu:70791.
UCSCiuc008eor.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiSYHM_MOUSE
AccessioniPrimary (citable) accession number: Q99KK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 1, 2001
Last modified: September 27, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families