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Protein

Probable histidine--tRNA ligase, mitochondrial

Gene

Hars2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable histidine--tRNA ligase, mitochondrial (EC:6.1.1.21)
Alternative name(s):
Histidine--tRNA ligase-like
Histidyl-tRNA synthetase
Short name:
HisRS
Gene namesi
Name:Hars2
Synonyms:Harsl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1918041. Hars2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3131MitochondrionSequence analysisAdd
BLAST
Chaini32 – 505474Probable histidine--tRNA ligase, mitochondrialPRO_0000254584Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei66 – 661PhosphoserineCombined sources
Modified residuei443 – 4431N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99KK9.
MaxQBiQ99KK9.
PaxDbiQ99KK9.
PeptideAtlasiQ99KK9.
PRIDEiQ99KK9.

PTM databases

iPTMnetiQ99KK9.
PhosphoSiteiQ99KK9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000019143.
ExpressionAtlasiQ99KK9. baseline and differential.
GenevisibleiQ99KK9. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000117231.

Structurei

3D structure databases

ProteinModelPortaliQ99KK9.
SMRiQ99KK9. Positions 54-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1936. Eukaryota.
COG0124. LUCA.
GeneTreeiENSGT00390000005922.
HOGENOMiHOG000018075.
HOVERGENiHBG002731.
InParanoidiQ99KK9.
KOiK01892.
OMAiLHYCENM.
OrthoDBiEOG091G05P3.
TreeFamiTF300652.

Family and domain databases

CDDicd00859. HisRS_anticodon. 1 hit.
Gene3Di3.40.50.800. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
IPR033656. HisRS_anticodon.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99KK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHLGPLRRR AWAALLGQLL RPPSTVCTRG CHSQVAKAVL TSEQLKSHQE
60 70 80 90 100
KPNFVIKVPK GTRDLSPQQM VVREKILDKI ISCFKRHGAK GLDTPAFELK
110 120 130 140 150
EMLTEKYEDN FGLMYDLKDQ GGELLSLRYD LTVPFARYLA MNKLKKMKRY
160 170 180 190 200
QVGKVWRRES PAIAQGRYRE FCQCDFDIAG EFDPMIPDAE CLRIMCEILS
210 220 230 240 250
GLQLGDFLIK VNDRRVVDGI FAVCGVPESK LRTICSSMDK LDKMSWEGVR
260 270 280 290 300
HEMVAKKGLA PEVADRIGDF VQYHGGISLV EDLFKDPRLS QSQLALQGLG
310 320 330 340 350
DLKLLFEYLR LFGIADKISL DLSLARGLDY YTGVIYEAVL LESPAQAGKE
360 370 380 390 400
TLSVGSVAAG GRYDNLVAQF DPKGHHVPCV GLSIGVERIF YLVEQKMKMS
410 420 430 440 450
GEKVRTTETQ VFVATPQKNF LQERLKIIAE LWDAGIKAEM LYKNNPKLLT
460 470 480 490 500
QLHYCEKADI PLMVIIGEQE RNEGVIKLRS VASREEVTIN RESLVAEIQK

RLSES
Length:505
Mass (Da):56,985
Last modified:June 1, 2001 - v1
Checksum:i1865426DF408DA1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC004596 mRNA. Translation: AAH04596.1.
CCDSiCCDS29165.1.
RefSeqiNP_542367.1. NM_080636.2.
UniGeneiMm.282700.

Genome annotation databases

EnsembliENSMUST00000152954; ENSMUSP00000117231; ENSMUSG00000019143.
GeneIDi70791.
KEGGimmu:70791.
UCSCiuc008eor.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC004596 mRNA. Translation: AAH04596.1.
CCDSiCCDS29165.1.
RefSeqiNP_542367.1. NM_080636.2.
UniGeneiMm.282700.

3D structure databases

ProteinModelPortaliQ99KK9.
SMRiQ99KK9. Positions 54-504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000117231.

PTM databases

iPTMnetiQ99KK9.
PhosphoSiteiQ99KK9.

Proteomic databases

EPDiQ99KK9.
MaxQBiQ99KK9.
PaxDbiQ99KK9.
PeptideAtlasiQ99KK9.
PRIDEiQ99KK9.

Protocols and materials databases

DNASUi70791.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000152954; ENSMUSP00000117231; ENSMUSG00000019143.
GeneIDi70791.
KEGGimmu:70791.
UCSCiuc008eor.2. mouse.

Organism-specific databases

CTDi23438.
MGIiMGI:1918041. Hars2.

Phylogenomic databases

eggNOGiKOG1936. Eukaryota.
COG0124. LUCA.
GeneTreeiENSGT00390000005922.
HOGENOMiHOG000018075.
HOVERGENiHBG002731.
InParanoidiQ99KK9.
KOiK01892.
OMAiLHYCENM.
OrthoDBiEOG091G05P3.
TreeFamiTF300652.

Miscellaneous databases

PROiQ99KK9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019143.
ExpressionAtlasiQ99KK9. baseline and differential.
GenevisibleiQ99KK9. MM.

Family and domain databases

CDDicd00859. HisRS_anticodon. 1 hit.
Gene3Di3.40.50.800. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-ligase.
IPR004516. HisRS/HisZ.
IPR033656. HisRS_anticodon.
[Graphical view]
PANTHERiPTHR11476. PTHR11476. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
[Graphical view]
PIRSFiPIRSF001549. His-tRNA_synth. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00442. hisS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYHM_MOUSE
AccessioniPrimary (citable) accession number: Q99KK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.