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Protein

Dynactin subunit 2

Gene

Dctn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: MGI
  • melanosome transport Source: MGI
  • mitotic cell cycle Source: MGI
  • mitotic spindle organization Source: MGI
  • protein localization to centrosome Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-2132295 MHC class II antigen presentation
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Dynactin subunit 2
Alternative name(s):
50 kDa dynein-associated polypeptide
Dynactin complex 50 kDa subunit
Short name:
DCTN-50
Growth cone membrane protein 23-48K
Short name:
GMP23-48K
p50 dynamitin
Gene namesi
Name:Dctn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:107733 Dctn2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000798222 – 402Dynactin subunit 2Add BLAST401

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei6PhosphotyrosineBy similarity1
Modified residuei83PhosphoserineCombined sources1
Modified residuei86PhosphotyrosineCombined sources1
Modified residuei134PhosphothreonineBy similarity1
Modified residuei321PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99KJ8
MaxQBiQ99KJ8
PaxDbiQ99KJ8
PRIDEiQ99KJ8

2D gel databases

REPRODUCTION-2DPAGEiQ99KJ8
UCD-2DPAGEiQ99KJ8

PTM databases

iPTMnetiQ99KJ8
PhosphoSitePlusiQ99KJ8

Expressioni

Developmental stagei

Present at high levels in both cytoplasmic and membrane-associated forms in neonates. Levels of membrane-associated form are greatly reduced in the adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000025410
CleanExiMM_DCTN2
ExpressionAtlasiQ99KJ8 baseline and differential
GenevisibleiQ99KJ8 MM

Interactioni

Subunit structurei

Subunit of dynactin, a multiprotein complex associated with dynein. Interacts with ECPAS, DYNAP and MAPRE1 (By similarity). Interacts with BICD2 (PubMed:11483508, PubMed:22956769). Interacts with CEP135 (PubMed:14983524).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213590, 22 interactors
CORUMiQ99KJ8
IntActiQ99KJ8, 28 interactors
MINTiQ99KJ8
STRINGi10090.ENSMUSP00000026479

Structurei

3D structure databases

ProteinModelPortaliQ99KJ8
SMRiQ99KJ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili99 – 132Sequence analysisAdd BLAST34
Coiled coili215 – 245Sequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the dynactin subunit 2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3958 Eukaryota
ENOG410XSZK LUCA
GeneTreeiENSGT00390000003427
HOGENOMiHOG000007303
HOVERGENiHBG051323
InParanoidiQ99KJ8
KOiK10424
OMAiSGEYEML
OrthoDBiEOG091G0D0D
PhylomeDBiQ99KJ8
TreeFamiTF105247

Family and domain databases

InterProiView protein in InterPro
IPR028133 Dynamitin
PANTHERiPTHR15346 PTHR15346, 1 hit
PfamiView protein in Pfam
PF04912 Dynamitin, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99KJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPKYADLP GIARNEPDVY ETSDLPEDDQ AEFDAEELSS TSVEHIIVNP
60 70 80 90 100
NAAYDKFKDK RVGTKGLDFS DRIGKTKRTG YESGDYEMLG EGLGVKETPQ
110 120 130 140 150
QKYQRLLHEV QELTTEVEKI KTTVKESATE EKLTPVVLAK QLAALKQQLV
160 170 180 190 200
ASHLEKLLGP DAAINLADPD GALAKRLLLQ LEATKSSKGS SGGKATAGAP
210 220 230 240 250
PDSSLVTYEL HSRPEQDKFS QAAKVAELEK RLTELEATVR CDQDAQNPLS
260 270 280 290 300
AGLQGACLME TVELLQAKVS ALDLAVLDQV EARLQSVLGK VNEIAKHKAS
310 320 330 340 350
VEDADTQNKV HQLYETIQRW SPVASTLPEL VQRLVTIKQL HEQAMQFGQL
360 370 380 390 400
LTHLDTTQQM MASSLKDNTA LLTQVQTTMR ENLATVEGNF ASIDARMKRL

GK
Length:402
Mass (Da):44,117
Last modified:January 23, 2007 - v3
Checksum:iEA0D8E04E92726AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC004613 mRNA Translation: AAH04613.1
CCDSiCCDS24234.1
RefSeqiNP_081427.1, NM_027151.2
UniGeneiMm.167537

Genome annotation databases

EnsembliENSMUST00000026479; ENSMUSP00000026479; ENSMUSG00000025410
GeneIDi69654
KEGGimmu:69654
UCSCiuc007hiu.2 mouse

Similar proteinsi

Entry informationi

Entry nameiDCTN2_MOUSE
AccessioniPrimary (citable) accession number: Q99KJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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