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Protein

Dynactin subunit 2

Gene

Dctn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: MGI
  • melanosome transport Source: MGI
  • microtubule-based process Source: InterPro
  • mitotic nuclear division Source: MGI
  • protein localization to centrosome Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-MMU-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynactin subunit 2
Alternative name(s):
50 kDa dynein-associated polypeptide
Dynactin complex 50 kDa subunit
Short name:
DCTN-50
Growth cone membrane protein 23-48K
Short name:
GMP23-48K
p50 dynamitin
Gene namesi
Name:Dctn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:107733. Dctn2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 402401Dynactin subunit 2PRO_0000079822Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei6 – 61PhosphotyrosineBy similarity
Modified residuei83 – 831PhosphoserineCombined sources
Modified residuei86 – 861PhosphotyrosineCombined sources
Modified residuei321 – 3211PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99KJ8.
MaxQBiQ99KJ8.
PaxDbiQ99KJ8.
PRIDEiQ99KJ8.

2D gel databases

REPRODUCTION-2DPAGEQ99KJ8.
UCD-2DPAGEQ99KJ8.

PTM databases

iPTMnetiQ99KJ8.
PhosphoSiteiQ99KJ8.

Expressioni

Developmental stagei

Present at high levels in both cytoplasmic and membrane-associated forms in neonates. Levels of membrane-associated form are greatly reduced in the adult.1 Publication

Gene expression databases

BgeeiQ99KJ8.
CleanExiMM_DCTN2.
ExpressionAtlasiQ99KJ8. baseline and differential.
GenevisibleiQ99KJ8. MM.

Interactioni

Subunit structurei

Subunit of dynactin, a multiprotein complex associated with dynein. Interacts with ECM29, DYNAP and MAPRE1 (By similarity). Interacts with BICD2 and CEP135.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213590. 22 interactions.
IntActiQ99KJ8. 26 interactions.
MINTiMINT-1866067.
STRINGi10090.ENSMUSP00000026479.

Structurei

3D structure databases

ProteinModelPortaliQ99KJ8.
SMRiQ99KJ8. Positions 1-85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili99 – 13234Sequence analysisAdd
BLAST
Coiled coili215 – 24531Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the dynactin subunit 2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3958. Eukaryota.
ENOG410XSZK. LUCA.
GeneTreeiENSGT00390000003427.
HOGENOMiHOG000007303.
HOVERGENiHBG051323.
InParanoidiQ99KJ8.
KOiK10424.
OMAiKYQRLVN.
OrthoDBiEOG7JT6WG.
PhylomeDBiQ99KJ8.
TreeFamiTF105247.

Family and domain databases

InterProiIPR028133. Dynamitin.
[Graphical view]
PANTHERiPTHR15346:SF0. PTHR15346:SF0. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99KJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPKYADLP GIARNEPDVY ETSDLPEDDQ AEFDAEELSS TSVEHIIVNP
60 70 80 90 100
NAAYDKFKDK RVGTKGLDFS DRIGKTKRTG YESGDYEMLG EGLGVKETPQ
110 120 130 140 150
QKYQRLLHEV QELTTEVEKI KTTVKESATE EKLTPVVLAK QLAALKQQLV
160 170 180 190 200
ASHLEKLLGP DAAINLADPD GALAKRLLLQ LEATKSSKGS SGGKATAGAP
210 220 230 240 250
PDSSLVTYEL HSRPEQDKFS QAAKVAELEK RLTELEATVR CDQDAQNPLS
260 270 280 290 300
AGLQGACLME TVELLQAKVS ALDLAVLDQV EARLQSVLGK VNEIAKHKAS
310 320 330 340 350
VEDADTQNKV HQLYETIQRW SPVASTLPEL VQRLVTIKQL HEQAMQFGQL
360 370 380 390 400
LTHLDTTQQM MASSLKDNTA LLTQVQTTMR ENLATVEGNF ASIDARMKRL

GK
Length:402
Mass (Da):44,117
Last modified:January 23, 2007 - v3
Checksum:iEA0D8E04E92726AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC004613 mRNA. Translation: AAH04613.1.
CCDSiCCDS24234.1.
RefSeqiNP_081427.1. NM_027151.2.
UniGeneiMm.167537.

Genome annotation databases

EnsembliENSMUST00000026479; ENSMUSP00000026479; ENSMUSG00000025410.
GeneIDi69654.
KEGGimmu:69654.
UCSCiuc007hiu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC004613 mRNA. Translation: AAH04613.1.
CCDSiCCDS24234.1.
RefSeqiNP_081427.1. NM_027151.2.
UniGeneiMm.167537.

3D structure databases

ProteinModelPortaliQ99KJ8.
SMRiQ99KJ8. Positions 1-85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213590. 22 interactions.
IntActiQ99KJ8. 26 interactions.
MINTiMINT-1866067.
STRINGi10090.ENSMUSP00000026479.

PTM databases

iPTMnetiQ99KJ8.
PhosphoSiteiQ99KJ8.

2D gel databases

REPRODUCTION-2DPAGEQ99KJ8.
UCD-2DPAGEQ99KJ8.

Proteomic databases

EPDiQ99KJ8.
MaxQBiQ99KJ8.
PaxDbiQ99KJ8.
PRIDEiQ99KJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026479; ENSMUSP00000026479; ENSMUSG00000025410.
GeneIDi69654.
KEGGimmu:69654.
UCSCiuc007hiu.2. mouse.

Organism-specific databases

CTDi10540.
MGIiMGI:107733. Dctn2.

Phylogenomic databases

eggNOGiKOG3958. Eukaryota.
ENOG410XSZK. LUCA.
GeneTreeiENSGT00390000003427.
HOGENOMiHOG000007303.
HOVERGENiHBG051323.
InParanoidiQ99KJ8.
KOiK10424.
OMAiKYQRLVN.
OrthoDBiEOG7JT6WG.
PhylomeDBiQ99KJ8.
TreeFamiTF105247.

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811436. COPI-independent Golgi-to-ER retrograde traffic.
R-MMU-8854518. AURKA Activation by TPX2.

Miscellaneous databases

ChiTaRSiDctn2. mouse.
PROiQ99KJ8.
SOURCEiSearch...

Gene expression databases

BgeeiQ99KJ8.
CleanExiMM_DCTN2.
ExpressionAtlasiQ99KJ8. baseline and differential.
GenevisibleiQ99KJ8. MM.

Family and domain databases

InterProiIPR028133. Dynamitin.
[Graphical view]
PANTHERiPTHR15346:SF0. PTHR15346:SF0. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. "The presence of the 50-kDa subunit of dynactin complex in the nerve growth cone."
    Abe T.K., Tanaka H., Iwanaga T., Odani S., Kuwano R.
    Biochem. Biophys. Res. Commun. 233:295-299(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 66-75; 78-92; 103-117; 157-171; 195-217 AND 310-321, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Tissue: Brain.
  3. Lubec G., Yang J.W., Zigmond M.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 320-333.
    Tissue: Brain.
  4. "Mammalian Golgi-associated Bicaudal-D2 functions in the dynein-dynactin pathway by interacting with these complexes."
    Hoogenraad C.C., Akhmanova A., Howell S.A., Dortland B.R., de Zeeuw C.I., Willemsen R., Visser P., Grosveld F., Galjart N.
    EMBO J. 20:4041-4054(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BICD2.
  5. "Interaction of Cep135 with a p50 dynactin subunit in mammalian centrosomes."
    Uetake Y., Terada Y., Matuliene J., Kuriyama R.
    Cell Motil. Cytoskeleton 58:53-66(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CEP135.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-86, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83 AND SER-321, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiDCTN2_MOUSE
AccessioniPrimary (citable) accession number: Q99KJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.