Reviewed,
UniProtKB/Swiss-Prot Q99KI0 (ACON_MOUSE)
Last modified
November 3, 2009.
Version 67.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Aconitate hydratase, mitochondrial Short name=Aconitase EC=4.2.1.3 Alternative name(s): Citrate hydro-lyase | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 780 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | Citrate = isocitrate. |
| Cofactor | Binds 1 4Fe-4S cluster per subunit. Binding of a 3Fe-4S cluster leads to an inactive enzyme By similarity. |
| Pathway | |
| Subunit structure | Monomer By similarity. |
| Subcellular location | Mitochondrion By similarity. |
| Post-translational modification | Acetylation of Lys-50 is observed in liver mitochondria from fasted mice but not from fed mice. |
| Sequence similarities | Belongs to the aconitase/IPM isomerase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Tricarboxylic acid cycle |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | 4Fe-4S Iron Iron-sulfur Metal-binding |
| Molecular function | Lyase |
| PTM | Acetylation Isopeptide bond Pyrrolidone carboxylic acid Ubl conjugation |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | mitochondrion Inferred from direct assay. Source: MGI |
| Molecular function | 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activityInferred from direct assay. Source: MGI iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 27 | 27 | Mitochondrion By similarity | ||||||
| Chain | 28 – 780 | 753 | Aconitate hydratase, mitochondrial | PRO_0000000542 | |||||
Sites | |||||||||
| Metal binding | 385 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 448 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 451 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 28 | 1 | Pyrrolidone carboxylic acid By similarity | ||||||
| Modified residue | 50 | 1 | N6-acetyllysine Ref.4 | ||||||
| Modified residue | 573 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 605 | 1 | N6-acetyllysine By similarity | ||||||
| Cross-link | 144 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5 | |||||||
Experimental info | |||||||||
| Sequence conflict | 7 – 8 | 2 | LV → P in BAE25770. Ref.1 | ||||||
| Sequence conflict | 618 | 1 | L → F in AAH94462. Ref.2 | ||||||
| Sequence conflict | 758 | 1 | F → L in BAE29252. Ref.1 | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| AK143917 mRNA. Translation: BAE25602.1. AK144207 mRNA. Translation: BAE25770.1. AK145511 mRNA. Translation: BAE26479.1. AK150027 mRNA. Translation: BAE29252.1. AK165411 mRNA. Translation: BAE38169.1. BC004645 mRNA. Translation: AAH04645.1. BC094462 mRNA. Translation: AAH94462.1. | |
| IPI | IPI00116074. |
| RefSeq | NP_542364.1. |
| UniGene | Mm.154581 |
3D structure databases | |
| HSSP | HSSP built from PDB template 7ACN based on UniProtKB P16276. |
| SMR | Q99KI0. Positions 29-780. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q99KI0. 6 interactions. |
| STRING | Q99KI0. |
PTM databases | |
| PhosphoSite | Q99KI0. |
2-D gel databases | |
| REPRODUCTION-2DPAGE | Q99KI0. |
Proteomic databases | |
| PRIDE | Q99KI0. |
Genome annotation databases | |
| Ensembl | ENSMUST00000023116; ENSMUSP00000023116; ENSMUSG00000022477; Mus musculus. [Genome view] |
| GeneID | 11429. |
| KEGG | mmu:11429. |
| NMPDR | fig|10090.3.peg.30319. |
| UCSC | uc007wxp.1. mouse. |
Organism-specific databases | |
| CTD | 11429. |
| MGI | MGI:87880. Aco2. |
Phylogenomic databases | |
| HOGENOM | Q99KI0. |
| HOVERGEN | Q99KI0. |
| OMA | PGKPLKC. |
Enzyme and pathway databases | |
| BRENDA | 4.2.1.3. 244. |
Gene expression databases | |
| ArrayExpress | Q99KI0. |
| Bgee | Q99KI0. |
| CleanEx | MM_ACO2. |
| Genevestigator | Q99KI0. |
| GermOnline | ENSMUSG00000022477. Mus musculus. |
Family and domain databases | |
| InterPro | IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR006248. Aconitase_mito-like. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view] |
| Gene3D | G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit. |
| PANTHER | PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF5. Aconitase_mito. 1 hit. |
| Pfam | PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view] |
| PRINTS | PR00415. ACONITASE. |
| ProDom | PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR01340. aconitase_mito. 1 hit. |
| PROSITE | PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 278704. |
| SOURCE | Search... |
Entry information
| Entry name | ACON_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q99KI0 Secondary accession number(s): Q3UDK9 Q505P4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


