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Protein

Serine/threonine-protein kinase 24

Gene

Stk24

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. In association with STK26 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation. Regulates also cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. Acts as a key regulator of axon regeneration in the optic nerve and radial nerve (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity, Mn2+By similarity, Co2+By similarity, Zn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53ATPPROSITE-ProRule annotation1
Active sitei144Proton acceptorPROSITE-ProRule annotation1
Metal bindingi149MagnesiumBy similarity1
Metal bindingi162MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 38ATPPROSITE-ProRule annotation9
Nucleotide bindingi100 – 102ATPPROSITE-ProRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-111465 Apoptotic cleavage of cellular proteins
R-MMU-75153 Apoptotic execution phase

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 24 (EC:2.7.11.1)
Alternative name(s):
Mammalian STE20-like protein kinase 3
Short name:
MST-3
STE20-like kinase MST3
Cleaved into the following 2 chains:
Alternative name(s):
Mammalian STE20-like protein kinase 3 N-terminal
Short name:
MST3/N
Alternative name(s):
Mammalian STE20-like protein kinase 3 C-terminal
Short name:
MST3/C
Gene namesi
Name:Stk24
Synonyms:Mst3, Stk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2385007 Stk24

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000867122 – 431Serine/threonine-protein kinase 24Add BLAST430
ChainiPRO_00004136202 – 313Serine/threonine-protein kinase 24 35 kDa subunitBy similarityAdd BLAST312
ChainiPRO_0000413621314 – 431Serine/threonine-protein kinase 24 12 kDa subunitBy similarityAdd BLAST118

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei4PhosphoserineCombined sources1
Modified residuei178Phosphothreonine; by autocatalysisBy similarity1
Modified residuei308PhosphoserineBy similarity1

Post-translational modificationi

Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis (By similarity).By similarity
Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-178 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei313 – 314Cleavage; by caspase-3, caspase-7 and caspase-8By similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99KH8
MaxQBiQ99KH8
PaxDbiQ99KH8
PRIDEiQ99KH8

PTM databases

iPTMnetiQ99KH8
PhosphoSitePlusiQ99KH8

Expressioni

Gene expression databases

BgeeiENSMUSG00000063410
CleanExiMM_STK24
ExpressionAtlasiQ99KH8 baseline and differential
GenevisibleiQ99KH8 MM

Interactioni

Subunit structurei

Monomer. Interacts with CTTNBP2NL. Interacts with RIPOR1 (via C-terminus); this interaction occurs in a PDCD10-dependent and Rho-independent manner. Interacts with PDCD10; this interaction is required for the association of STK24 with RIPOR1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230135, 2 interactors
IntActiQ99KH8, 1 interactor
MINTiQ99KH8
STRINGi10090.ENSMUSP00000078746

Structurei

3D structure databases

ProteinModelPortaliQ99KH8
SMRiQ99KH8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 274Protein kinasePROSITE-ProRule annotationAdd BLAST251

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi266 – 280Bipartite nuclear localization signalBy similarityAdd BLAST15
Motifi323 – 374Nuclear export signal (NES)By similarityAdd BLAST52

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0201 Eukaryota
ENOG410XP9G LUCA
GeneTreeiENSGT00900000140794
HOGENOMiHOG000234203
HOVERGENiHBG108518
InParanoidiQ99KH8
KOiK08838
OMAiDTDGQAS
OrthoDBiEOG091G0F24
PhylomeDBiQ99KH8
TreeFamiTF354217

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99KH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHSPVQSGL PGMQNLKADP EELFTKLEKI GKGSFGEVFK GIDNRTQKVV
60 70 80 90 100
AIKIIDLEEA EDEIEDIQQE ITVLSQCDSP YVTKYYGSYL KDTKLWIIME
110 120 130 140 150
YLGGGSALDL LEPGPLDEIQ IATILREILK GLDYLHSEKK IHRDIKAANV
160 170 180 190 200
LLSEHGEVKL ADFGVAGQLT DTQIKRNTFV GTPFWMAPEV IKQSAYDSKA
210 220 230 240 250
DIWSLGITAI ELAKGEPPHS ELHPMKVLFL IPKNNPPTLE GNYSKPLKEF
260 270 280 290 300
VEACLNKEPS FRPTAKELLK HKFIIRNAKK TSYLTELIDR YKRWKAEQSH
310 320 330 340 350
EDSSSEDSDV ETDGQASGGS DSGDWIFTIR EKDPKNLENG TLQLSDLERN
360 370 380 390 400
KMKDIPKRPF SQCLSTIISP LFAELKEKSQ ACGGNLGSIE ELRGAIYLAE
410 420 430
EACPGISDTM VAQLVQRLQR YSLSGGGASA H
Length:431
Mass (Da):47,954
Last modified:June 1, 2001 - v1
Checksum:iBB0A47D9701AEB00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC004650 mRNA Translation: AAH04650.1
CCDSiCCDS37016.1
RefSeqiNP_663440.1, NM_145465.2
UniGeneiMm.390756
Mm.442081
Mm.472866

Genome annotation databases

EnsembliENSMUST00000079817; ENSMUSP00000078746; ENSMUSG00000063410
GeneIDi223255
KEGGimmu:223255
UCSCiuc007vac.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSTK24_MOUSE
AccessioniPrimary (citable) accession number: Q99KH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

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