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Protein

Serine/threonine-protein kinase 24

Gene

Stk24

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. Acts as a key regulator of axon regeneration in the optic nerve and radial nerve (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity, Mn2+By similarity, Co2+By similarity, Zn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531ATPPROSITE-ProRule annotation
Active sitei144 – 1441Proton acceptorPROSITE-ProRule annotation
Metal bindingi149 – 1491MagnesiumBy similarity
Metal bindingi162 – 1621MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 389ATPPROSITE-ProRule annotation
Nucleotide bindingi100 – 1023ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-75153. Apoptotic execution phase.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 24 (EC:2.7.11.1)
Alternative name(s):
Mammalian STE20-like protein kinase 3
Short name:
MST-3
STE20-like kinase MST3
Cleaved into the following 2 chains:
Alternative name(s):
Mammalian STE20-like protein kinase 3 N-terminal
Short name:
MST3/N
Alternative name(s):
Mammalian STE20-like protein kinase 3 C-terminal
Short name:
MST3/C
Gene namesi
Name:Stk24
Synonyms:Mst3, Stk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2385007. Stk24.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Membrane By similarity

  • Note: The truncated form (MST3/N) translocates to the nucleus. Colocalizes with STK38L in the membrane (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 431430Serine/threonine-protein kinase 24PRO_0000086712Add
BLAST
Chaini2 – 313312Serine/threonine-protein kinase 24 35 kDa subunitBy similarityPRO_0000413620Add
BLAST
Chaini314 – 431118Serine/threonine-protein kinase 24 12 kDa subunitBy similarityPRO_0000413621Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei4 – 41PhosphoserineCombined sources
Modified residuei178 – 1781Phosphothreonine; by autocatalysisBy similarity
Modified residuei308 – 3081PhosphoserineBy similarity

Post-translational modificationi

Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis (By similarity).By similarity
Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-178 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei313 – 3142Cleavage; by caspase-3, caspase-7 and caspase-8By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99KH8.
MaxQBiQ99KH8.
PaxDbiQ99KH8.
PRIDEiQ99KH8.

PTM databases

iPTMnetiQ99KH8.
PhosphoSiteiQ99KH8.

Expressioni

Gene expression databases

BgeeiQ99KH8.
CleanExiMM_STK24.
ExpressionAtlasiQ99KH8. baseline and differential.
GenevisibleiQ99KH8. MM.

Interactioni

Subunit structurei

Monomer. Interacts with CTTNBP2NL.By similarity

Protein-protein interaction databases

BioGridi230135. 1 interaction.
IntActiQ99KH8. 1 interaction.
MINTiMINT-4135908.
STRINGi10090.ENSMUSP00000078746.

Structurei

3D structure databases

ProteinModelPortaliQ99KH8.
SMRiQ99KH8. Positions 9-338, 360-422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 274251Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi266 – 28015Bipartite nuclear localization signalBy similarityAdd
BLAST
Motifi323 – 37452Nuclear export signal (NES)By similarityAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0201. Eukaryota.
ENOG410XP9G. LUCA.
GeneTreeiENSGT00810000125395.
HOGENOMiHOG000234203.
HOVERGENiHBG108518.
InParanoidiQ99KH8.
KOiK08838.
OMAiRAQLWGL.
OrthoDBiEOG77T14Q.
PhylomeDBiQ99KH8.
TreeFamiTF354217.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99KH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHSPVQSGL PGMQNLKADP EELFTKLEKI GKGSFGEVFK GIDNRTQKVV
60 70 80 90 100
AIKIIDLEEA EDEIEDIQQE ITVLSQCDSP YVTKYYGSYL KDTKLWIIME
110 120 130 140 150
YLGGGSALDL LEPGPLDEIQ IATILREILK GLDYLHSEKK IHRDIKAANV
160 170 180 190 200
LLSEHGEVKL ADFGVAGQLT DTQIKRNTFV GTPFWMAPEV IKQSAYDSKA
210 220 230 240 250
DIWSLGITAI ELAKGEPPHS ELHPMKVLFL IPKNNPPTLE GNYSKPLKEF
260 270 280 290 300
VEACLNKEPS FRPTAKELLK HKFIIRNAKK TSYLTELIDR YKRWKAEQSH
310 320 330 340 350
EDSSSEDSDV ETDGQASGGS DSGDWIFTIR EKDPKNLENG TLQLSDLERN
360 370 380 390 400
KMKDIPKRPF SQCLSTIISP LFAELKEKSQ ACGGNLGSIE ELRGAIYLAE
410 420 430
EACPGISDTM VAQLVQRLQR YSLSGGGASA H
Length:431
Mass (Da):47,954
Last modified:June 1, 2001 - v1
Checksum:iBB0A47D9701AEB00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC004650 mRNA. Translation: AAH04650.1.
CCDSiCCDS37016.1.
RefSeqiNP_663440.1. NM_145465.2.
UniGeneiMm.390756.
Mm.442081.
Mm.472866.

Genome annotation databases

EnsembliENSMUST00000079817; ENSMUSP00000078746; ENSMUSG00000063410.
GeneIDi223255.
KEGGimmu:223255.
UCSCiuc007vac.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC004650 mRNA. Translation: AAH04650.1.
CCDSiCCDS37016.1.
RefSeqiNP_663440.1. NM_145465.2.
UniGeneiMm.390756.
Mm.442081.
Mm.472866.

3D structure databases

ProteinModelPortaliQ99KH8.
SMRiQ99KH8. Positions 9-338, 360-422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230135. 1 interaction.
IntActiQ99KH8. 1 interaction.
MINTiMINT-4135908.
STRINGi10090.ENSMUSP00000078746.

PTM databases

iPTMnetiQ99KH8.
PhosphoSiteiQ99KH8.

Proteomic databases

EPDiQ99KH8.
MaxQBiQ99KH8.
PaxDbiQ99KH8.
PRIDEiQ99KH8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079817; ENSMUSP00000078746; ENSMUSG00000063410.
GeneIDi223255.
KEGGimmu:223255.
UCSCiuc007vac.1. mouse.

Organism-specific databases

CTDi8428.
MGIiMGI:2385007. Stk24.

Phylogenomic databases

eggNOGiKOG0201. Eukaryota.
ENOG410XP9G. LUCA.
GeneTreeiENSGT00810000125395.
HOGENOMiHOG000234203.
HOVERGENiHBG108518.
InParanoidiQ99KH8.
KOiK08838.
OMAiRAQLWGL.
OrthoDBiEOG77T14Q.
PhylomeDBiQ99KH8.
TreeFamiTF354217.

Enzyme and pathway databases

ReactomeiR-MMU-75153. Apoptotic execution phase.

Miscellaneous databases

ChiTaRSiStk24. mouse.
NextBioi376675.
PROiQ99KH8.
SOURCEiSearch...

Gene expression databases

BgeeiQ99KH8.
CleanExiMM_STK24.
ExpressionAtlasiQ99KH8. baseline and differential.
GenevisibleiQ99KH8. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  2. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  3. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiSTK24_MOUSE
AccessioniPrimary (citable) accession number: Q99KH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: June 1, 2001
Last modified: May 11, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.