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Protein

Docking protein 4

Gene

Dok4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells (By similarity).By similarity

GO - Molecular functioni

  • receptor signaling protein activity Source: MGI

GO - Biological processi

  • MAPK cascade Source: MGI
  • nervous system development Source: MGI
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Docking protein 4
Alternative name(s):
Downstream of tyrosine kinase 4
Gene namesi
Name:Dok4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2148865. Dok4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Docking protein 4PRO_0000187275Add
BLAST

Post-translational modificationi

Phosphorylated on tyrosine residues in response to insulin, IGF1 or RET stimulation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99KE3.
PaxDbiQ99KE3.
PRIDEiQ99KE3.

PTM databases

iPTMnetiQ99KE3.
PhosphoSiteiQ99KE3.

Expressioni

Tissue specificityi

Widely expressed. Highest levels in heart, lung and kidney.1 Publication

Developmental stagei

At E12.5 and E13.5, expressed in the central and peripheral nervous system and in endothelia. In the nervous system, expression localized to the ventral portion of the neural tube and the dorsal root and cranial sensory ganglia. Expression confined to neurons.1 Publication

Gene expression databases

BgeeiQ99KE3.
CleanExiMM_DOK4.
GenevisibleiQ99KE3. MM.

Interactioni

Subunit structurei

Interacts with RET and TEK/TIE2. Interaction with RET is mediated through the PTB domain and requires phosphorylation of RET 'Tyr-1062'.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043554.

Structurei

3D structure databases

ProteinModelPortaliQ99KE3.
SMRiQ99KE3. Positions 134-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 112106PHAdd
BLAST
Domaini132 – 237106IRS-type PTBPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi265 – 27511DKFBH motifAdd
BLAST

Domaini

PTB domain mediates receptor interaction.By similarity

Sequence similaritiesi

Belongs to the DOK family. Type B subfamily.Curated
Contains 1 IRS-type PTB domain.PROSITE-ProRule annotation
Contains 1 PH domain.Curated

Phylogenomic databases

eggNOGiKOG4047. Eukaryota.
ENOG410XS2S. LUCA.
GeneTreeiENSGT00730000110348.
HOGENOMiHOG000247025.
HOVERGENiHBG002356.
InParanoidiQ99KE3.
OMAiDTHNPRV.
OrthoDBiEOG7GTT3W.
PhylomeDBiQ99KE3.
TreeFamiTF324994.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR002404. IRS_PTB.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF02174. IRS. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00310. PTBI. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS51064. IRS_PTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99KE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATNFNDIVK QGYVKMKSRK LGIYRRCWLV FRKSSSKGPQ RLEKYPDEKS
60 70 80 90 100
VCLRGCPKVT EISNVKCVTR LPKETKRQAV AIIFTDDSAR TFTCDSELEA
110 120 130 140 150
EEWYKTLSVE CLGSRLNDIS LGEPDLLAPG VQCEQTDRFN VFLLPCPNLD
160 170 180 190 200
VYGECKLQIT HENIYLWDIH NPRVKLVSWP LCSLRRYGRD ATRFTFEAGR
210 220 230 240 250
MCDAGEGLYT FQTQEGEQIY QRVHSATLAI AEQHKRVLLE MEKNVRLLNK
260 270 280 290 300
GTEHYSYPCT PTAMLPRSAY WHHITGSQNI AEASSYGESY GAAQASSETD
310 320
LLNRFILLKP KPSQEDSSEA KTPAQ
Length:325
Mass (Da):37,040
Last modified:June 1, 2001 - v1
Checksum:iB925D9F11BA7093A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF418207 mRNA. Translation: AAL14626.1.
AK032352 mRNA. Translation: BAC27829.1.
AK043670 mRNA. Translation: BAC31613.1.
AK142621 mRNA. Translation: BAE25134.1.
AK156485 mRNA. Translation: BAE33728.1.
BC004705 mRNA. Translation: AAH04705.1.
CCDSiCCDS40443.1.
RefSeqiNP_444476.1. NM_053246.3.
XP_006530642.1. XM_006530579.2.
UniGeneiMm.203949.

Genome annotation databases

EnsembliENSMUST00000046461; ENSMUSP00000043554; ENSMUSG00000040631.
GeneIDi114255.
KEGGimmu:114255.
UCSCiuc009mxf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF418207 mRNA. Translation: AAL14626.1.
AK032352 mRNA. Translation: BAC27829.1.
AK043670 mRNA. Translation: BAC31613.1.
AK142621 mRNA. Translation: BAE25134.1.
AK156485 mRNA. Translation: BAE33728.1.
BC004705 mRNA. Translation: AAH04705.1.
CCDSiCCDS40443.1.
RefSeqiNP_444476.1. NM_053246.3.
XP_006530642.1. XM_006530579.2.
UniGeneiMm.203949.

3D structure databases

ProteinModelPortaliQ99KE3.
SMRiQ99KE3. Positions 134-232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043554.

PTM databases

iPTMnetiQ99KE3.
PhosphoSiteiQ99KE3.

Proteomic databases

MaxQBiQ99KE3.
PaxDbiQ99KE3.
PRIDEiQ99KE3.

Protocols and materials databases

DNASUi114255.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046461; ENSMUSP00000043554; ENSMUSG00000040631.
GeneIDi114255.
KEGGimmu:114255.
UCSCiuc009mxf.2. mouse.

Organism-specific databases

CTDi55715.
MGIiMGI:2148865. Dok4.

Phylogenomic databases

eggNOGiKOG4047. Eukaryota.
ENOG410XS2S. LUCA.
GeneTreeiENSGT00730000110348.
HOGENOMiHOG000247025.
HOVERGENiHBG002356.
InParanoidiQ99KE3.
OMAiDTHNPRV.
OrthoDBiEOG7GTT3W.
PhylomeDBiQ99KE3.
TreeFamiTF324994.

Miscellaneous databases

PROiQ99KE3.
SOURCEiSearch...

Gene expression databases

BgeeiQ99KE3.
CleanExiMM_DOK4.
GenevisibleiQ99KE3. MM.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR002404. IRS_PTB.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF02174. IRS. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00310. PTBI. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS51064. IRS_PTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel p62dok family members, dok-4 and dok-5, are substrates of the c-Ret receptor tyrosine kinase and mediate neuronal differentiation."
    Grimm J., Sachs M., Britsch S., Di Cesare S., Schwarz-Romond T., Alitalo K., Birchmeier W.
    J. Cell Biol. 154:345-354(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RET, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Brain cortex, Mammary gland, Olfactory bulb and Spleen.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiDOK4_MOUSE
AccessioniPrimary (citable) accession number: Q99KE3
Secondary accession number(s): Q3U0X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.