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Protein

Hydroxyacylglutathione hydrolase, mitochondrial

Gene

Hagh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

Caution

Only one single gene encoding glyoxalase II has been identified in vertebrates. In yeast and higher plants, separate genes encode the cytosolic and mitochondrial forms of glyoxalase II.Curated

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (Glo1), Lactoylglutathione lyase (Glo1)
  2. Hydroxyacylglutathione hydrolase, mitochondrial (Hagh)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi103Zinc 1; via tele nitrogenBy similarity1
Metal bindingi105Zinc 1; via pros nitrogenBy similarity1
Metal bindingi107Zinc 2By similarity1
Metal bindingi108Zinc 2; via tele nitrogenBy similarity1
Metal bindingi159Zinc 1; via tele nitrogenBy similarity1
Metal bindingi183Zinc 1By similarity1
Metal bindingi183Zinc 2By similarity1
Metal bindingi222Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • hydroxyacylglutathione hydrolase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

SABIO-RKiQ99KB8
UniPathwayiUPA00619; UER00676

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase, mitochondrial (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Gene namesi
Name:Hagh
Synonyms:Glo2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:95745 Hagh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 24MitochondrionSequence analysisAdd BLAST24
ChainiPRO_000019234325 – 309Hydroxyacylglutathione hydrolase, mitochondrialAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei90N6-acetyllysineCombined sources1
Modified residuei117N6-acetyllysineCombined sources1
Modified residuei230N6-acetyllysine; alternateBy similarity1
Modified residuei230N6-succinyllysine; alternateCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ99KB8
PeptideAtlasiQ99KB8
PRIDEiQ99KB8

2D gel databases

REPRODUCTION-2DPAGEiQ99KB8

PTM databases

iPTMnetiQ99KB8
PhosphoSitePlusiQ99KB8

Expressioni

Gene expression databases

CleanExiMM_HAGH

Interactioni

Protein-protein interaction databases

IntActiQ99KB8, 5 interactors
MINTiQ99KB8
STRINGi10090.ENSMUSP00000113051

Structurei

3D structure databases

ProteinModelPortaliQ99KB8
SMRiQ99KB8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni192 – 194Substrate bindingBy similarity3
Regioni222 – 224Substrate bindingBy similarity3
Regioni298 – 301Substrate bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0813 Eukaryota
COG0491 LUCA
HOGENOMiHOG000058041
HOVERGENiHBG001152
InParanoidiQ99KB8
KOiK01069
PhylomeDBiQ99KB8

Family and domain databases

CDDicd07723 hydroxyacylglutathione_hydrola, 1 hit
Gene3Di3.60.15.10, 1 hit
HAMAPiMF_01374 Glyoxalase_2, 1 hit
InterProiView protein in InterPro
IPR035680 Clx_II_MBL
IPR032282 HAGH_C
IPR017782 Hydroxyacylglutathione_Hdrlase
IPR001279 Metallo-B-lactamas
IPR036866 RibonucZ/Hydroxyglut_hydro
PfamiView protein in Pfam
PF16123 HAGH_C, 1 hit
PF00753 Lactamase_B, 1 hit
PIRSFiPIRSF005457 Glx, 1 hit
SMARTiView protein in SMART
SM00849 Lactamase_B, 1 hit
SUPFAMiSSF56281 SSF56281, 1 hit
TIGRFAMsiTIGR03413 GSH_gloB, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q99KB8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLGRGSLCL RSLSALGATC ARRGLGQALL GLSLCHSDFR KNLTVQQDIM
60 70 80 90 100
KVELLPALTD NYMYLIIDED TQEAAIVDPV QPQKVIEAAK KHHVKLTTVL
110 120 130 140 150
TTHHHWDHAG GNEKLVKLEP GLKVYGGDDR IGALTHKVTH LSTLQVGSLS
160 170 180 190 200
VKCLSTPCHT SGHICYFVSK PGSSEPSAVF TGDTLFVAGC GKFYEGTADE
210 220 230 240 250
MYKALLEVLG RLPPDTKVYC GHEYTVNNLK FARHVEPGNA AIQEKLAWAK
260 270 280 290 300
EKYAIGEPTV PSTLAEEFTY NPFMRVKEKT VQQHAGETDP VTTMRAIRRE

KDQFKVPRD
Length:309
Mass (Da):34,084
Last modified:September 1, 2009 - v2
Checksum:iAC2C17D7AB6E4FBA
GO
Isoform 2 (identifier: Q99KB8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: Produced by alternative splicing. Also produced by alternative initiation at Met-50 of isoform 1. Alternative initiation has been proven in human.
Show »
Length:260
Mass (Da):28,901
Checksum:i94FD38ACD64684BF
GO

Sequence cautioni

The sequence AAH04749 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH19817 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE27615 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE29657 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE30223 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE31462 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93H → R in BAE27615 (PubMed:16141072).Curated1
Sequence conflicti93H → R in BAE29657 (PubMed:16141072).Curated1
Sequence conflicti93H → R in BAE30223 (PubMed:16141072).Curated1
Sequence conflicti93H → R in BAE31462 (PubMed:16141072).Curated1
Sequence conflicti93H → R in BAE38377 (PubMed:16141072).Curated1
Sequence conflicti147G → E in BAE38377 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0379311 – 49Missing in isoform 2. 2 PublicationsAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147022 mRNA Translation: BAE27615.1 Different initiation.
AK150557 mRNA Translation: BAE29657.1 Different initiation.
AK151231 mRNA Translation: BAE30223.1 Different initiation.
AK152742 mRNA Translation: BAE31462.1 Different initiation.
AK165777 mRNA Translation: BAE38377.1
BC004749 mRNA Translation: AAH04749.1 Different initiation.
BC019817 mRNA Translation: AAH19817.1 Different initiation.
CCDSiCCDS28499.2 [Q99KB8-1]
CCDS50021.1 [Q99KB8-2]
RefSeqiNP_001153098.1, NM_001159626.1
NP_077246.2, NM_024284.2
XP_006523723.1, XM_006523660.2
XP_006523724.1, XM_006523661.1
UniGeneiMm.43784

Genome annotation databases

GeneIDi14651
KEGGimmu:14651

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLO2_MOUSE
AccessioniPrimary (citable) accession number: Q99KB8
Secondary accession number(s): Q3TMR4, Q3UCF6, Q8WUR9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: September 1, 2009
Last modified: June 20, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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