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Q99K85

- SERC_MOUSE

UniProt

Q99K85 - SERC_MOUSE

Protein

Phosphoserine aminotransferase

Gene

Psat1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.By similarity

    Catalytic activityi

    O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate.
    4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate.

    Cofactori

    Binds 1 pyridoxal phosphate per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei45 – 451L-glutamateBy similarity
    Binding sitei107 – 1071Pyridoxal phosphateBy similarity
    Binding sitei156 – 1561Pyridoxal phosphateBy similarity
    Binding sitei176 – 1761Pyridoxal phosphateBy similarity
    Binding sitei199 – 1991Pyridoxal phosphateBy similarity

    GO - Molecular functioni

    1. O-phospho-L-serine:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. L-serine biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Serine biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_199119. Serine biosynthesis.
    UniPathwayiUPA00135; UER00197.
    UPA00244; UER00311.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoserine aminotransferase (EC:2.6.1.52)
    Short name:
    PSAT
    Alternative name(s):
    Endometrial progesterone-induced protein
    Short name:
    EPIP
    Phosphohydroxythreonine aminotransferase
    Gene namesi
    Name:Psat1
    Synonyms:Psa, Psat
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:2183441. Psat1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Phosphoserine aminotransferasePRO_0000150136Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei51 – 511N6-acetyllysineBy similarity
    Modified residuei127 – 1271N6-acetyllysine1 Publication
    Modified residuei200 – 2001N6-(pyridoxal phosphate)lysineBy similarity
    Modified residuei269 – 2691N6-acetyllysineBy similarity
    Modified residuei318 – 3181N6-acetyllysineBy similarity
    Modified residuei323 – 3231N6-acetyllysineBy similarity
    Modified residuei333 – 3331N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ99K85.
    PaxDbiQ99K85.
    PRIDEiQ99K85.

    PTM databases

    PhosphoSiteiQ99K85.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99K85.
    BgeeiQ99K85.
    CleanExiMM_PSAT1.
    GenevestigatoriQ99K85.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi223231. 1 interaction.
    IntActiQ99K85. 2 interactions.
    MINTiMINT-4134236.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99K85.
    SMRiQ99K85. Positions 17-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni79 – 802Pyridoxal phosphate bindingBy similarity
    Regioni241 – 2422Pyridoxal phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1932.
    GeneTreeiENSGT00390000015177.
    HOGENOMiHOG000088965.
    HOVERGENiHBG001218.
    InParanoidiQ99K85.
    KOiK00831.
    OMAiFAAIPMN.
    OrthoDBiEOG7KQ220.
    PhylomeDBiQ99K85.
    TreeFamiTF312975.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00160. SerC_aminotrans_5.
    InterProiIPR000192. Aminotrans_V/Cys_dSase.
    IPR020578. Aminotrans_V_PyrdxlP_BS.
    IPR022278. Pser_aminoTfrase.
    IPR003248. Pser_aminoTfrase_subgr.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00266. Aminotran_5. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000525. SerC. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01364. serC_1. 1 hit.
    PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99K85-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEATKQVVNF GPGPAKLPHS VLLEIQKQLL DYRGLGISVL EMSHRSSDFA    50
    KIIGNTENLV RELLAVPNNY KVIFVQGGGS GQFSAVPLNL IGLKAGRSAD 100
    YVVTGAWSAK AAEEAKKFGT VNIVHPKLGS YTKIPDPSTW NLNPDASYVY 150
    FCANETVHGV EFDFVPDVKG AVLVCDMSSN FLSRPVDVSK FGVIFAGAQK 200
    NVGSAGVTVV IVRDDLLGFS LRECPSVLDY KVQAGNNSLY NTPPCFSIYV 250
    MGMVLEWIKN NGGAAAMEKL SSIKSQMIYE IIDNSQGFYV CPVERQNRSR 300
    MNIPFRIGNA KGDEALEKRF LDKAVELNMI SLKGHRSVGG IRASLYNAVT 350
    TEDVEKLAAF MKNFLEMHQL 370
    Length:370
    Mass (Da):40,473
    Last modified:June 1, 2001 - v1
    Checksum:i6CF6B80CFDA1974B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF259674 mRNA. Translation: AAK69389.1.
    BC004827 mRNA. Translation: AAH04827.1.
    CCDSiCCDS29681.1.
    RefSeqiNP_803155.1. NM_177420.2.
    UniGeneiMm.289936.

    Genome annotation databases

    EnsembliENSMUST00000025542; ENSMUSP00000025542; ENSMUSG00000024640.
    GeneIDi107272.
    KEGGimmu:107272.
    UCSCiuc008gwp.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF259674 mRNA. Translation: AAK69389.1 .
    BC004827 mRNA. Translation: AAH04827.1 .
    CCDSi CCDS29681.1.
    RefSeqi NP_803155.1. NM_177420.2.
    UniGenei Mm.289936.

    3D structure databases

    ProteinModelPortali Q99K85.
    SMRi Q99K85. Positions 17-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 223231. 1 interaction.
    IntActi Q99K85. 2 interactions.
    MINTi MINT-4134236.

    PTM databases

    PhosphoSitei Q99K85.

    Proteomic databases

    MaxQBi Q99K85.
    PaxDbi Q99K85.
    PRIDEi Q99K85.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025542 ; ENSMUSP00000025542 ; ENSMUSG00000024640 .
    GeneIDi 107272.
    KEGGi mmu:107272.
    UCSCi uc008gwp.2. mouse.

    Organism-specific databases

    CTDi 29968.
    MGIi MGI:2183441. Psat1.

    Phylogenomic databases

    eggNOGi COG1932.
    GeneTreei ENSGT00390000015177.
    HOGENOMi HOG000088965.
    HOVERGENi HBG001218.
    InParanoidi Q99K85.
    KOi K00831.
    OMAi FAAIPMN.
    OrthoDBi EOG7KQ220.
    PhylomeDBi Q99K85.
    TreeFami TF312975.

    Enzyme and pathway databases

    UniPathwayi UPA00135 ; UER00197 .
    UPA00244 ; UER00311 .
    Reactomei REACT_199119. Serine biosynthesis.

    Miscellaneous databases

    ChiTaRSi PSAT1. mouse.
    NextBioi 358656.
    PROi Q99K85.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99K85.
    Bgeei Q99K85.
    CleanExi MM_PSAT1.
    Genevestigatori Q99K85.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00160. SerC_aminotrans_5.
    InterProi IPR000192. Aminotrans_V/Cys_dSase.
    IPR020578. Aminotrans_V_PyrdxlP_BS.
    IPR022278. Pser_aminoTfrase.
    IPR003248. Pser_aminoTfrase_subgr.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00266. Aminotran_5. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000525. SerC. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR01364. serC_1. 1 hit.
    PROSITEi PS00595. AA_TRANSFER_CLASS_5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Sato M., Oguma T., Tsujimoto K., Tadakuma T.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 17-27; 52-94; 191-200; 223-231 AND 343-356, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.
    4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-127, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiSERC_MOUSE
    AccessioniPrimary (citable) accession number: Q99K85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3