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Protein

Mediator of RNA polymerase II transcription subunit 24

Gene

Med24

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Required for basal and activator-dependent transcription.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 24
Alternative name(s):
Mediator complex subunit 24
Thyroid hormone receptor-associated protein 4
Thyroid hormone receptor-associated protein complex 100 kDa component
Short name:
Trap100
Short name:
mTRAP100
Gene namesi
Name:Med24
Synonyms:D11Ertd307e, Thrap4, Trap100
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1344385. Med24.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
  • ubiquitin ligase complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 987987Mediator of RNA polymerase II transcription subunit 24PRO_0000305912Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei860 – 8601PhosphoserineCombined sources
Modified residuei871 – 8711PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99K74.
MaxQBiQ99K74.
PaxDbiQ99K74.
PeptideAtlasiQ99K74.
PRIDEiQ99K74.

PTM databases

iPTMnetiQ99K74.
PhosphoSiteiQ99K74.

Expressioni

Tissue specificityi

Expressed in the adrenal gland, brain, epididymis, heart, kidney, liver, ovary, pancreas, prostate, skeletal muscle, small intestine, spleen, stomach, testis and thymus.2 Publications

Developmental stagei

Expressed throughout development; expression levels drop immediately after birth. Strongly expressed throughout the primitive nervous system, the hepatic primoridium and the earliest limb buds.1 Publication

Gene expression databases

BgeeiENSMUSG00000017210.
CleanExiMM_MED24.
ExpressionAtlasiQ99K74. baseline and differential.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR (By similarity). Interacts with MED1 and MED10.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204840. 2 interactions.
IntActiQ99K74. 4 interactions.
STRINGi10090.ENSMUSP00000017354.

Structurei

3D structure databases

ProteinModelPortaliQ99K74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi128 – 1325LXXLL motif 1
Motifi344 – 3485LXXLL motif 2
Motifi446 – 4505LXXLL motif 3
Motifi555 – 5595LXXLL motif 4
Motifi786 – 7905LXXLL motif 5
Motifi855 – 8595LXXLL motif 6

Sequence similaritiesi

Belongs to the Mediator complex subunit 24 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF72. Eukaryota.
ENOG410YPDT. LUCA.
GeneTreeiENSGT00390000016438.
HOGENOMiHOG000293419.
HOVERGENiHBG055588.
InParanoidiQ99K74.
KOiK15167.
PhylomeDBiQ99K74.
TreeFamiTF323565.

Family and domain databases

InterProiIPR021429. Mediator_Med24_N.
[Graphical view]
PfamiPF11277. Med24_N. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99K74-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLAEALLEQA
60 70 80 90 100
MIGPSPNPLI LSYLKYAISS QMVSCSSVLT AISKFDDFSR DLCVQALLDI
110 120 130 140 150
MDMFCDRLSC HGKAEECIGL CRALLSALHW LLRCTAASAE RLQEGLEAGT
160 170 180 190 200
PAPGEKQLAL CLQCLEKTLS STKNRALLHI AKLEEASSWT AIEHSLLKLG
210 220 230 240 250
EILANLSNPQ LRSQAERCGT LIRSIPSMLS VHSEQLHKTG FPTIHALILL
260 270 280 290 300
EGTMNLTGEM QPLVEQLMMV KRMQHIPTPL FVLEIWKACF VGLIESPEGT
310 320 330 340 350
QELKWTAFTY LKIPQVLVKL KKYFHGEKDF TEDVNCAFEF LLKLTPLLDK
360 370 380 390 400
ADQRCNCDCT NFLLQECNKQ GLLSEVNFAS LVGKRTADRD PQLKSSENAN
410 420 430 440 450
IQPNPGLILR AEPTVTNILK TMDADHSKSP EGLLGVLGHM LSGKSLDLLL
460 470 480 490 500
AAAAATGKLK SFARKFINLN EFTTHGSGES TKTASVRALL FDISFLMLCH
510 520 530 540 550
VAQTYGSEVI LSESSSGEEV PFFETWMQTC MPEEGKILNP DHPCFRPDST
560 570 580 590 600
KVESLVALLN NSSEMKLVQM KWHEACLSIS AAILEILNAW ENGVLAFESI
610 620 630 640 650
QKITDNIKGK VCSLAVCAVA WLVAHVRMLG LDEREKSLQM IRQLAGPLYS
660 670 680 690 700
ENTLQFYNER VVIMNSILEH MCADVLQQTA TQIKFPSTGV DTMPYWNLLP
710 720 730 740 750
PKRPIKEVLT DIFAKVLEKG WVDSRSIHIL DTLLHMGGVY WFCNNLIKEL
760 770 780 790 800
LKETRKEHTL RAVQLLYSIF CLDMQQVTLV LLGHILPGLL TDSSKWHSLM
810 820 830 840 850
DPPGTALAKL AVWCALSSYS SHKGQASSRQ KKRHREDIED YVSLFPVEDM
860 870 880 890 900
QPSKLMRLLS SSDDDANILS SPTDRSMNSS LSASQLHTVN MRDPLNRVLA
910 920 930 940 950
NLFLLISSIL GSRTAGPHTQ FVQWFMEECV GCLEQDSRGS ILQFMPFTTV
960 970 980
SELVKVSAMS SPKVVLAITD LSLPLGRQVA AKAIAAL
Length:987
Mass (Da):109,985
Last modified:June 1, 2001 - v1
Checksum:i713C6A02C3C0B294
GO
Isoform 2 (identifier: Q99K74-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.
     187-187: S → SLHTSQGLGQGGTRANQPTA

Show »
Length:956
Mass (Da):105,942
Checksum:i41FC6F4D850D6540
GO
Isoform 3 (identifier: Q99K74-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     661-758: VVIMNSILEH...ELLKETRKEH → SVPRPRQVCG...LVCVSVSRGF
     759-987: Missing.

Note: No experimental confirmation available.
Show »
Length:758
Mass (Da):83,879
Checksum:iF09A51692BE1598B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261N → K in BAB32051 (PubMed:16141072).Curated
Sequence conflicti503 – 5031Q → H in AAD42776 (PubMed:10406464).Curated
Sequence conflicti541 – 5411D → E in AAD42776 (PubMed:10406464).Curated
Sequence conflicti566 – 5661K → N in AAD42776 (PubMed:10406464).Curated
Sequence conflicti608 – 6081K → N in AAD42776 (PubMed:10406464).Curated
Sequence conflicti699 – 6991L → F in AAD42776 (PubMed:10406464).Curated
Sequence conflicti888 – 8881T → R in AAD42776 (PubMed:10406464).Curated
Sequence conflicti940 – 9401S → T in AAD42776 (PubMed:10406464).Curated
Sequence conflicti963 – 9631K → E in AAD42776 (PubMed:10406464).Curated
Sequence conflicti970 – 9701D → H in AAD42776 (PubMed:10406464).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5050Missing in isoform 2. 2 PublicationsVSP_028355Add
BLAST
Alternative sequencei187 – 1871S → SLHTSQGLGQGGTRANQPTA in isoform 2. 2 PublicationsVSP_028356
Alternative sequencei661 – 75898VVIMN…TRKEH → SVPRPRQVCGRESAPPGTNI PPHALGLRFPGSRSATTSSS IQAPTSPPCLAAEAVHIQGS WTLAPSFAFPYHSLAFLVTH SLSWLSRCLVCVSVSRGF in isoform 3. 1 PublicationVSP_028357Add
BLAST
Alternative sequencei759 – 987229Missing in isoform 3. 1 PublicationVSP_028358Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126543 mRNA. Translation: AAD42776.1.
AF483498 mRNA. Translation: AAL90772.1.
AF483499 mRNA. Translation: AAL90773.1.
AK020269 mRNA. Translation: BAB32051.1.
AK154618 mRNA. Translation: BAE32717.1.
AL590963 Genomic DNA. Translation: CAM46193.1.
BC005409 mRNA. Translation: AAH05409.1.
CCDSiCCDS25361.2. [Q99K74-1]
RefSeqiNP_035999.2. NM_011869.2. [Q99K74-1]
XP_006533368.1. XM_006533305.2. [Q99K74-1]
UniGeneiMm.246493.

Genome annotation databases

EnsembliENSMUST00000017354; ENSMUSP00000017354; ENSMUSG00000017210. [Q99K74-1]
GeneIDi23989.
KEGGimmu:23989.
UCSCiuc007lgz.1. mouse. [Q99K74-2]
uc007lha.1. mouse. [Q99K74-1]
uc007lhc.1. mouse. [Q99K74-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126543 mRNA. Translation: AAD42776.1.
AF483498 mRNA. Translation: AAL90772.1.
AF483499 mRNA. Translation: AAL90773.1.
AK020269 mRNA. Translation: BAB32051.1.
AK154618 mRNA. Translation: BAE32717.1.
AL590963 Genomic DNA. Translation: CAM46193.1.
BC005409 mRNA. Translation: AAH05409.1.
CCDSiCCDS25361.2. [Q99K74-1]
RefSeqiNP_035999.2. NM_011869.2. [Q99K74-1]
XP_006533368.1. XM_006533305.2. [Q99K74-1]
UniGeneiMm.246493.

3D structure databases

ProteinModelPortaliQ99K74.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204840. 2 interactions.
IntActiQ99K74. 4 interactions.
STRINGi10090.ENSMUSP00000017354.

PTM databases

iPTMnetiQ99K74.
PhosphoSiteiQ99K74.

Proteomic databases

EPDiQ99K74.
MaxQBiQ99K74.
PaxDbiQ99K74.
PeptideAtlasiQ99K74.
PRIDEiQ99K74.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017354; ENSMUSP00000017354; ENSMUSG00000017210. [Q99K74-1]
GeneIDi23989.
KEGGimmu:23989.
UCSCiuc007lgz.1. mouse. [Q99K74-2]
uc007lha.1. mouse. [Q99K74-1]
uc007lhc.1. mouse. [Q99K74-3]

Organism-specific databases

CTDi9862.
MGIiMGI:1344385. Med24.

Phylogenomic databases

eggNOGiENOG410IF72. Eukaryota.
ENOG410YPDT. LUCA.
GeneTreeiENSGT00390000016438.
HOGENOMiHOG000293419.
HOVERGENiHBG055588.
InParanoidiQ99K74.
KOiK15167.
PhylomeDBiQ99K74.
TreeFamiTF323565.

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSiMed24. mouse.
PROiQ99K74.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017210.
CleanExiMM_MED24.
ExpressionAtlasiQ99K74. baseline and differential.

Family and domain databases

InterProiIPR021429. Mediator_Med24_N.
[Graphical view]
PfamiPF11277. Med24_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED24_MOUSE
AccessioniPrimary (citable) accession number: Q99K74
Secondary accession number(s): A3KFP0
, Q8R004, Q9D277, Q9WVF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.