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Protein

Protein regulator of cytokinesis 1

Gene

Prc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei377 – 3771TubulinBy similarity
Binding sitei387 – 3871TubulinBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

ReactomeiR-MMU-5625900. RHO GTPases activate CIT.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein regulator of cytokinesis 1Imported
Gene namesi
Name:Prc1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1858961. Prc1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Cytoplasmcytoskeletonspindle pole By similarity
  • Midbody By similarity

  • Note: Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles, and localizes with the cell midbody during cytokinesis. Colocalized with KIF20B in the nucleus of bladder carcinoma cells at the interphase. Colocalized with KIF20B in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 603603Protein regulator of cytokinesis 1CuratedPRO_0000229738Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei195 – 1951PhosphoserineBy similarity
Modified residuei470 – 4701Phosphothreonine; by CDK1By similarity
Modified residuei498 – 4981PhosphoserineBy similarity
Modified residuei510 – 5101PhosphoserineBy similarity
Modified residuei568 – 5681PhosphoserineBy similarity
Modified residuei598 – 5981PhosphoserineBy similarity
Modified residuei599 – 5991Phosphothreonine; by PLK1By similarity

Post-translational modificationi

Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization. Phosphorylation by CDK1 prevents PLK1-binding: upon degradation of CDK1 at anaphase and dephosphorylation, it is then phosphorylated by PLK1, leading to cytokinesis (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99K43.
MaxQBiQ99K43.
PaxDbiQ99K43.
PRIDEiQ99K43.

PTM databases

iPTMnetiQ99K43.
PhosphoSiteiQ99K43.

Expressioni

Developmental stagei

During the stages E11.5-E13.5 it is expressed in most embryonic tissues. Within the telencephalon, it is predominantly expressed inside the ventricular zone where expression reaches its peak at E15.5 and starts to decrease by E18.5. Expression is also observed in mitotically active cells outside the telencephalon, but not in adult brain.1 Publication

Gene expression databases

BgeeiQ99K43.
CleanExiMM_PRC1.
ExpressionAtlasiQ99K43. baseline and differential.
GenevisibleiQ99K43. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with the C-terminal Rho-GAP domain and the basic region of RACGAP1. The interaction with RACGAP1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. Interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis. Interacts with KIF14 and KIF20A. Interacts with PLK1. Interacts with KIF20B.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ99K43. 4 interactions.
MINTiMINT-1668946.
STRINGi10090.ENSMUSP00000129675.

Structurei

3D structure databases

ProteinModelPortaliQ99K43.
SMRiQ99K43. Positions 2-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 341341DimerizationBy similarityAdd
BLAST
Regioni342 – 466125Spectrin-foldBy similarityAdd
BLAST
Regioni467 – 603137Unstructured, Arg/Lys richBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili34 – 6532Sequence analysisAdd
BLAST
Coiled coili96 – 13641Sequence analysisAdd
BLAST
Coiled coili211 – 24636Sequence analysisAdd
BLAST
Coiled coili272 – 30433Sequence analysisAdd
BLAST
Coiled coili383 – 46381Sequence analysisAdd
BLAST

Domaini

Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.By similarity

Sequence similaritiesi

Belongs to the MAP65/ASE1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4302. Eukaryota.
ENOG410YZBK. LUCA.
GeneTreeiENSGT00390000009453.
HOGENOMiHOG000082534.
HOVERGENiHBG052963.
InParanoidiQ99K43.
KOiK16732.

Family and domain databases

InterProiIPR007145. MAP65_Ase1_PRC1.
IPR032921. PRC1.
[Graphical view]
PANTHERiPTHR19321. PTHR19321. 1 hit.
PTHR19321:SF1. PTHR19321:SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q99K43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRSEVLADE SITCLQKALT HLREIWELIG IPEEQRLQRT EVVKKHIKDL
60 70 80 90 100
LDRMIAEEES LRERLLKSIS ICQKELSTLC SELQVKPFQE EKDTTILQLE
110 120 130 140 150
KDLRTQVELM RKQKKERKQE LKLLQEQEQE LRDILCMPPC DVDSTSVPTL
160 170 180 190 200
EELKLFRQRV ATLRETKESR REEFVNIKKQ IILCMEELEH SPDTSFERDV
210 220 230 240 250
VCEDESAFCL SLENIATLQK LLKQLEMKKS QNEAECEGLR TQIRELWDRL
260 270 280 290 300
QIPEEEREPV EAIMTGSKTK IRNALKLEVD RLEELKMQNI KQVIEKIRVE
310 320 330 340 350
LAQFWDQCFY SQEQRQAFAP YYSEDYTENL LHLHDAEIVR LRNYYDVHKE
360 370 380 390 400
LFQGVQKWEE SWKLFLEFER KASDPGRFTN RGGNLLKEEK ERAKLQKTLP
410 420 430 440 450
KLEEELKARI EQWEQEHSTA FVVNGQKFME YVTEQWELHR LEKERAKQER
460 470 480 490 500
QLKNKKQTEA EMLYGSTPRT PSKRPGQTPK KSGKMNTTTM SSATPNSSIR
510 520 530 540 550
PVFGGSVYRS PMSRLPPSGS KSVVTSLCSG KKTPRAAQLR ANKENLDLNG
560 570 580 590 600
SILSGGYPGS TPLQHNCSIK SVASTYSEFS RELSKASRSD ATSRILNSTN

IQS
Length:603
Mass (Da):70,290
Last modified:July 27, 2011 - v2
Checksum:i267E2966FF237BD1
GO

Sequence cautioni

The sequence BAC26320.1 differs from that shown.Probable intron retention.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti347 – 3471V → A in AAH05475 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC109232 Genomic DNA. No translation available.
AC136740 Genomic DNA. No translation available.
BC005475 mRNA. Translation: AAH05475.1.
AK029140 mRNA. Translation: BAC26320.1. Sequence problems.
CCDSiCCDS71985.1.
RefSeqiNP_001272926.1. NM_001285997.1.
UniGeneiMm.227274.

Genome annotation databases

EnsembliENSMUST00000047558; ENSMUSP00000043379; ENSMUSG00000038943.
GeneIDi233406.
KEGGimmu:233406.
UCSCiuc009iaf.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC109232 Genomic DNA. No translation available.
AC136740 Genomic DNA. No translation available.
BC005475 mRNA. Translation: AAH05475.1.
AK029140 mRNA. Translation: BAC26320.1. Sequence problems.
CCDSiCCDS71985.1.
RefSeqiNP_001272926.1. NM_001285997.1.
UniGeneiMm.227274.

3D structure databases

ProteinModelPortaliQ99K43.
SMRiQ99K43. Positions 2-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99K43. 4 interactions.
MINTiMINT-1668946.
STRINGi10090.ENSMUSP00000129675.

PTM databases

iPTMnetiQ99K43.
PhosphoSiteiQ99K43.

Proteomic databases

EPDiQ99K43.
MaxQBiQ99K43.
PaxDbiQ99K43.
PRIDEiQ99K43.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047558; ENSMUSP00000043379; ENSMUSG00000038943.
GeneIDi233406.
KEGGimmu:233406.
UCSCiuc009iaf.3. mouse.

Organism-specific databases

CTDi9055.
MGIiMGI:1858961. Prc1.

Phylogenomic databases

eggNOGiKOG4302. Eukaryota.
ENOG410YZBK. LUCA.
GeneTreeiENSGT00390000009453.
HOGENOMiHOG000082534.
HOVERGENiHBG052963.
InParanoidiQ99K43.
KOiK16732.

Enzyme and pathway databases

ReactomeiR-MMU-5625900. RHO GTPases activate CIT.

Miscellaneous databases

ChiTaRSiPrc1. mouse.
NextBioi381696.
PROiQ99K43.
SOURCEiSearch...

Gene expression databases

BgeeiQ99K43.
CleanExiMM_PRC1.
ExpressionAtlasiQ99K43. baseline and differential.
GenevisibleiQ99K43. MM.

Family and domain databases

InterProiIPR007145. MAP65_Ase1_PRC1.
IPR032921. PRC1.
[Graphical view]
PANTHERiPTHR19321. PTHR19321. 1 hit.
PTHR19321:SF1. PTHR19321:SF1. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech IIImported.
    Tissue: Mammary glandImported.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-484.
    Strain: C57BL/6JImported.
    Tissue: SkinImported.
  4. "Expression of PTTG and prc1 genes during telencephalic neurogenesis."
    Tarabykin V., Britanova O., Fradkov A., Voss A., Katz L.S., Lukyanov S., Gruss P.
    Mech. Dev. 92:301-304(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.

Entry informationi

Entry nameiPRC1_MOUSE
AccessioniPrimary (citable) accession number: Q99K43
Secondary accession number(s): E9QPF0, Q8CE25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.