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Q99K41 (EMIL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
EMILIN-1
Alternative name(s):
Elastin microfibril interface-located protein 1
Short name=Elastin microfibril interfacer 1
Gene names
Name:Emilin1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1017 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity By similarity. May have a function in placenta formation and initial organogenesis and a later role in interstitial connective tissue.

Subunit structure

Homotrimer associated through a moderately stable interaction of the C-terminal globular C1q domains, allowing the nucleation of the triple helix and then a further quaternary assembly to higher-order polymers via intermolecular disulfide bonds. Interacts with EMILIN2 By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix. Note: Found mainly at the interface between amorphous elastin and microfibrils.

Developmental stage

Detectable in morula and blastocyst. First expressed in ectoplacental cone in embryos of 6.5 days and in extraembryonic visceral endoderm at 7.5 days. Expressed also in the allantois. Expression in the ectoplacental cone-derived secondary trophoblast giant cells and spongiotrophoblast is strong up to 11.5 days and then declines. In the embryo, high levels are initially expressed in blood vessels, perineural mesenchyme and somites at 8.5 days. Later on, intense expression is identified in the mesenchymal component of organs anlage (ie lung and liver) and different mesenchymal condensations (ie limb bud and branchial arches). At late gestation expression is widely distributed in interstitial connective tissue and smooth muscle cell-rich tissues. Ref.3

Sequence similarities

Contains 1 C1q domain.

Contains 1 collagen-like domain.

Contains 1 EMI domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TGFB1P072002EBI-906561,EBI-907660From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 1017994EMILIN-1
PRO_0000007816

Regions

Domain56 – 13378EMI
Domain815 – 86551Collagen-like
Domain867 – 1014148C1q
Coiled coil171 – 21141 Potential
Coiled coil237 – 26630 Potential
Coiled coil310 – 37465 Potential
Coiled coil519 – 57355 Potential
Coiled coil676 – 69722 Potential
Coiled coil789 – 80921 Potential
Compositional bias269 – 2757Poly-Gly

Amino acid modifications

Glycosylation1561N-linked (GlcNAc...) Potential
Glycosylation4161N-linked (GlcNAc...) Potential
Glycosylation4561N-linked (GlcNAc...) Potential
Glycosylation5621N-linked (GlcNAc...) Potential
Glycosylation6591N-linked (GlcNAc...) Potential
Glycosylation7671N-linked (GlcNAc...) Potential
Glycosylation7951N-linked (GlcNAc...) Potential
Disulfide bond60 ↔ 123 By similarity
Disulfide bond87 ↔ 94 By similarity
Disulfide bond122 ↔ 131 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99K41 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 2EF903204DB9C88F

FASTA1,017107,585
        10         20         30         40         50         60 
MAPRALWSCY LCCLLTIATE AASYPPRGYS LYTGGTGALS PGGPQAQNSP RPASRHRNWC 

        70         80         90        100        110        120 
AYVVTRTVSC VLEDGVETIV KPDYQPCGWG QPHCSRSIMY RSFLRPRYRV AYKTVTDMEW 

       130        140        150        160        170        180 
RCCQGYGGDD CGEGPASVLG PAPSTPLPRP RPVRPNLSGS SAGSHLSGLG GEGPVESEKV 

       190        200        210        220        230        240 
QQLERQVKSL TKELQGLRGV LQGMNGRLAE DVQRAVDTVF NGRQQPADAA ARPGVHETLS 

       250        260        270        280        290        300 
EIQQQLQLLD NRVSTHDQEL GHLNNHHNGG PGGGGRASGP VPVPSGPSEE LLRQLERQLQ 

       310        320        330        340        350        360 
ESCSVCLTGL DGFRQQQQED RERLRTLEKL MSSMEERQQQ LVGPAMARRP PQECCPPELG 

       370        380        390        400        410        420 
RRVSELERRL DVVTGSLTVL SGRRGSELGG AAGQGGHPPG YTSLASRLSR LEDRFNSTLG 

       430        440        450        460        470        480 
PSEEQEKNWP GGPGRLGHWL PAAPGRLEKL EGLLANVSRE LGGRMDLLEE QVAGAVRTCG 

       490        500        510        520        530        540 
QICSGAPGEQ DSRVNEILSA LERRVLDSEG RLQLVGSGLH EAEAAGEAQQ AVLEGLQGLL 

       550        560        570        580        590        600 
SRLRERMDAQ EETAAEILLR LNLTAAQLSQ LEGLLQARGD EGCGACGGVQ EELGRLRDGV 

       610        620        630        640        650        660 
ERCSCPLLPP RGPGAGPGVG GPSRGPLDGF SVFGGSSGSA LQALQGELSE VILTFSSLND 

       670        680        690        700        710        720 
SLHELQTTVE GQGADLADLG ATKDSIISEI NRLQQEATEH VTESEERFRG LEEGQAQAGQ 

       730        740        750        760        770        780 
CPSLEGRLGR LEGVCERLDT VAGGLQGLRE GLSRHVAGLW AAVRESNSTS LTQAALLEKL 

       790        800        810        820        830        840 
LGGQAGLGRR LGALNNSLLL LEDRLQQLSL KDFTGPSGKA GPPGPPGLQG PSGPAGPPGP 

       850        860        870        880        890        900 
PGKDGQQGAI GPPGPQGEQG AEGAPAAPVP RVAFSAALSL PRSEPGTVPF DRVLLNDGGY 

       910        920        930        940        950        960 
YDPETGVFTA PLAGRYLLSA VLTGHRHEKV EAVLSRSNLG VARIDSGGYE PEGLENKPVA 

       970        980        990       1000       1010 
ESQPSPGALG VFSLILPLQV GDTVCIDLVM GQLAHSEEPL TIFSGALLYE DTELEQV 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Head.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
[3]"Expression of the EMILIN-1 gene during mouse development."
Braghetta P., Ferrari A., de Gemmis P., Zanetti M., Volpin D., Bonaldo P., Bressan G.M.
Matrix Biol. 21:603-609(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK029337 mRNA. Translation: BAC26403.1.
BC005481 mRNA. Translation: AAH05481.1.
CCDSCCDS19167.1.
RefSeqNP_598679.1. NM_133918.2.
UniGeneMm.286375.

3D structure databases

ProteinModelPortalQ99K41.
SMRQ99K41. Positions 868-1016.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid221560. 1 interaction.
IntActQ99K41. 5 interactions.
MINTMINT-4094392.

PTM databases

PhosphoSiteQ99K41.

Proteomic databases

PaxDbQ99K41.
PRIDEQ99K41.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000031055; ENSMUSP00000031055; ENSMUSG00000029163.
GeneID100952.
KEGGmmu:100952.
UCSCuc008wwm.1. mouse.

Organism-specific databases

CTD11117.
MGIMGI:1926189. Emilin1.

Phylogenomic databases

eggNOGNOG244192.
GeneTreeENSGT00660000095314.
HOGENOMHOG000112364.
HOVERGENHBG051473.
InParanoidQ99K41.
OMAQQEATEH.
OrthoDBEOG74R1RX.
PhylomeDBQ99K41.
TreeFamTF331033.

Gene expression databases

BgeeQ99K41.
CleanExMM_EMILIN1.
GenevestigatorQ99K41.

Family and domain databases

Gene3D2.60.120.40. 1 hit.
InterProIPR001073. C1q.
IPR008160. Collagen.
IPR011489. EMI_domain.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamPF00386. C1q. 1 hit.
PF01391. Collagen. 1 hit.
PF07546. EMI. 1 hit.
[Graphical view]
SUPFAMSSF49842. SSF49842. 1 hit.
PROSITEPS50871. C1Q. 1 hit.
PS51041. EMI. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio354704.
PROQ99K41.
SOURCESearch...

Entry information

Entry nameEMIL1_MOUSE
AccessionPrimary (citable) accession number: Q99K41
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot