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Q99K30

- ES8L2_MOUSE

UniProt

Q99K30 - ES8L2_MOUSE

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Protein

Epidermal growth factor receptor kinase substrate 8-like protein 2

Gene

Eps8l2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton (By similarity).By similarity

GO - Molecular functioni

  1. actin filament binding Source: MGI
  2. Rac guanyl-nucleotide exchange factor activity Source: Ensembl

GO - Biological processi

  1. positive regulation of ruffle assembly Source: Ensembl
  2. Rac protein signal transduction Source: MGI
  3. regulation of Rac GTPase activity Source: Ensembl
  4. Rho protein signal transduction Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 2
Short name:
EPS8-like protein 2
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 2
Short name:
EPS8-related protein 2
Gene namesi
Name:Eps8l2
Synonyms:Eps8r2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2138828. Eps8l2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. protein complex Source: Ensembl
  4. ruffle Source: MGI
  5. ruffle membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Epidermal growth factor receptor kinase substrate 8-like protein 2PRO_0000239085Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei306 – 3061Phosphothreonine1 Publication
Modified residuei573 – 5731PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99K30.
PaxDbiQ99K30.
PRIDEiQ99K30.

PTM databases

PhosphoSiteiQ99K30.

Expressioni

Tissue specificityi

Detected in fetal kidney, adrenal gland, salivary gland, stomach, gut, cartilage and skin. Detected in adult ovary, placenta, skin, adrenal gland, salivary gland, kidney, intestine and stomach.1 Publication

Gene expression databases

BgeeiQ99K30.
CleanExiMM_EPS8L2.
ExpressionAtlasiQ99K30. baseline and differential.
GenevestigatoriQ99K30.

Interactioni

Subunit structurei

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin (By similarity).By similarity

Protein-protein interaction databases

BioGridi221147. 1 interaction.
IntActiQ99K30. 2 interactions.
MINTiMINT-1860812.

Structurei

3D structure databases

ProteinModelPortaliQ99K30.
SMRiQ99K30. Positions 46-175, 498-552, 615-700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 202157PIDPROSITE-ProRule annotationAdd
BLAST
Domaini495 – 55460SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the EPS8 family.Curated
Contains 1 PID domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG263108.
GeneTreeiENSGT00390000003646.
HOGENOMiHOG000060324.
HOVERGENiHBG003090.
InParanoidiQ99K30.
KOiK17277.
OMAiVLQEAPW.
OrthoDBiEOG7TMZR9.
PhylomeDBiQ99K30.
TreeFamiTF313069.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_like_dom.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q99K30-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQSASMSCC PGAANGSLGR SDGVPRMSAK DLFEQRKKYS NSNVIMHETS
60 70 80 90 100
QYHVQHLATF IMDKSEAIAS VDDAIRKLVQ LSSKEKVWAQ EVLLQVNDKS
110 120 130 140 150
LRLLDVESQE ELENFPLPTV QHSQTVLNQL RYPSVLLLVC QDSDQNKPDI
160 170 180 190 200
HFFHCDEVEA ELVQEDIESA LADYRLGKKM RPQTLKGHQE KIRQRQSILP
210 220 230 240 250
PPQSPAPIPF QRQPGDSPQA KNRVGLPLPV PFSEPGYRRR ESQDEEPRAV
260 270 280 290 300
LAQRIEKETQ ILNCTLDDIE WFVARLQKAA EAFKQLNQRK KGKKKNKKGP
310 320 330 340 350
AEGVLTLRAR PPSEGEFVDC FQKTKLAINL LAKLQKHIQN PSAAELVHFL
360 370 380 390 400
FGPLDLIINT CGSPDIARSV SSPLLSTDAV SFLRGHLVPK EMTLWESLGE
410 420 430 440 450
TWMRPRSEWP REPQVPLYVP KFRSGWEPPL DVLQEAPWEV EGLASVPSDQ
460 470 480 490 500
LTPKNRLSVR HSPKHSLSSE SQAPEDIAPP GSSPHANRGY QPTPAMTKYV
510 520 530 540 550
KILYDFTARN ANELSVLKDE VLEVLEDGRQ WWKLRNRSGQ AGYVPCNILA
560 570 580 590 600
EARQEDVGAP LEQSGQKYWG PASPTHKLPP IFAGNKEELI HHMDEVNDEL
610 620 630 640 650
MKKISHIKTQ PQRNFRVERS QPVHLPLTFE SGPDEVRAWL EAKAFSARIV
660 670 680 690 700
ENLGILTGPQ LFSLNKEELK KVCGEEGSRV YSQLTVQKAF LEKQQSGSEL
710 720
EKLMSKIRRA EDSYTSQHTS PESEGAPHL
Length:729
Mass (Da):82,229
Last modified:June 1, 2001 - v1
Checksum:iA11DE82FF2C0BD18
GO
Isoform 2 (identifier: Q99K30-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     357-400: IINTCGSPDI...EMTLWESLGE → VTGAGTGRGR...LGSQEATVHP
     401-729: Missing.

Show »
Length:400
Mass (Da):44,740
Checksum:iDE7B8FA22B454B20
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti177 – 1771G → R in AAH09098. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei357 – 40044IINTC…ESLGE → VTGAGTGRGRGQQSDPHGFG VEETSAHLSILKLCLGSQEA TVHP in isoform 2. 1 PublicationVSP_019093Add
BLAST
Alternative sequencei401 – 729329Missing in isoform 2. 1 PublicationVSP_019094Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC005492 mRNA. Translation: AAH05492.1.
BC009098 mRNA. Translation: AAH09098.1.
CCDSiCCDS22009.1. [Q99K30-1]
RefSeqiNP_573454.2. NM_133191.2. [Q99K30-1]
UniGeneiMm.27451.

Genome annotation databases

EnsembliENSMUST00000026577; ENSMUSP00000026577; ENSMUSG00000025504. [Q99K30-1]
GeneIDi98845.
KEGGimmu:98845.
UCSCiuc009kkt.1. mouse. [Q99K30-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC005492 mRNA. Translation: AAH05492.1 .
BC009098 mRNA. Translation: AAH09098.1 .
CCDSi CCDS22009.1. [Q99K30-1 ]
RefSeqi NP_573454.2. NM_133191.2. [Q99K30-1 ]
UniGenei Mm.27451.

3D structure databases

ProteinModelPortali Q99K30.
SMRi Q99K30. Positions 46-175, 498-552, 615-700.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 221147. 1 interaction.
IntActi Q99K30. 2 interactions.
MINTi MINT-1860812.

PTM databases

PhosphoSitei Q99K30.

Proteomic databases

MaxQBi Q99K30.
PaxDbi Q99K30.
PRIDEi Q99K30.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026577 ; ENSMUSP00000026577 ; ENSMUSG00000025504 . [Q99K30-1 ]
GeneIDi 98845.
KEGGi mmu:98845.
UCSCi uc009kkt.1. mouse. [Q99K30-1 ]

Organism-specific databases

CTDi 64787.
MGIi MGI:2138828. Eps8l2.

Phylogenomic databases

eggNOGi NOG263108.
GeneTreei ENSGT00390000003646.
HOGENOMi HOG000060324.
HOVERGENi HBG003090.
InParanoidi Q99K30.
KOi K17277.
OMAi VLQEAPW.
OrthoDBi EOG7TMZR9.
PhylomeDBi Q99K30.
TreeFami TF313069.

Miscellaneous databases

NextBioi 353685.
PROi Q99K30.
SOURCEi Search...

Gene expression databases

Bgeei Q99K30.
CleanExi MM_EPS8L2.
ExpressionAtlasi Q99K30. baseline and differential.
Genevestigatori Q99K30.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR011993. PH_like_dom.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
SMARTi SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and FVB/N.
    Tissue: Mammary tumor.
  2. "The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway."
    Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G.
    Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiES8L2_MOUSE
AccessioniPrimary (citable) accession number: Q99K30
Secondary accession number(s): Q91VT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3