Q99K30 (ES8L2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 2
Short name=EPS8-like protein 2

Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 2
Short name=EPS8-related protein 2

Gene names

Name:	Eps8l2
Synonyms:	Eps8r2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton By similarity.
Subunit structure	Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Detected in fetal kidney, adrenal gland, salivary gland, stomach, gut, cartilage and skin. Detected in adult ovary, placenta, skin, adrenal gland, salivary gland, kidney, intestine and stomach. Ref.2
Sequence similarities	Belongs to the EPS8 family. Contains 1 PID domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rac protein signal transduction Inferred from sequence orthology Ref.2. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: Compara ruffle Inferred from sequence orthology Ref.2. Source: MGI
Molecular_function	actin filament binding Inferred from sequence orthology Ref.2. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99K30-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99K30-2)

The sequence of this isoform differs from the canonical sequence as follows:
     357-400: IINTCGSPDI...EMTLWESLGE → VTGAGTGRGR...LGSQEATVHP
     401-729: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 729	729	Epidermal growth factor receptor kinase substrate 8-like protein 2		PRO_0000239085
Regions
	Domain	46 – 202	157	PID
	Domain	495 – 554	60	SH3
Amino acid modifications
	Modified residue	242	1	Phosphoserine Ref.3
	Modified residue	483	1	Phosphoserine Ref.3
	Modified residue	573	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	357 – 400	44	IINTC…ESLGE → VTGAGTGRGRGQQSDPHGFG VEETSAHLSILKLCLGSQEA TVHP in isoform 2.		VSP_019093
	Alternative sequence	401 – 729	329	Missing in isoform 2.		VSP_019094
Experimental info
	Sequence conflict	177	1	G → R in AAH09098. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: A11DE82FF2C0BD18	FASTA	729	82,229
	10 20 30 40 50 60 MSQSASMSCC PGAANGSLGR SDGVPRMSAK DLFEQRKKYS NSNVIMHETS QYHVQHLATF 70 80 90 100 110 120 IMDKSEAIAS VDDAIRKLVQ LSSKEKVWAQ EVLLQVNDKS LRLLDVESQE ELENFPLPTV 130 140 150 160 170 180 QHSQTVLNQL RYPSVLLLVC QDSDQNKPDI HFFHCDEVEA ELVQEDIESA LADYRLGKKM 190 200 210 220 230 240 RPQTLKGHQE KIRQRQSILP PPQSPAPIPF QRQPGDSPQA KNRVGLPLPV PFSEPGYRRR 250 260 270 280 290 300 ESQDEEPRAV LAQRIEKETQ ILNCTLDDIE WFVARLQKAA EAFKQLNQRK KGKKKNKKGP 310 320 330 340 350 360 AEGVLTLRAR PPSEGEFVDC FQKTKLAINL LAKLQKHIQN PSAAELVHFL FGPLDLIINT 370 380 390 400 410 420 CGSPDIARSV SSPLLSTDAV SFLRGHLVPK EMTLWESLGE TWMRPRSEWP REPQVPLYVP 430 440 450 460 470 480 KFRSGWEPPL DVLQEAPWEV EGLASVPSDQ LTPKNRLSVR HSPKHSLSSE SQAPEDIAPP 490 500 510 520 530 540 GSSPHANRGY QPTPAMTKYV KILYDFTARN ANELSVLKDE VLEVLEDGRQ WWKLRNRSGQ 550 560 570 580 590 600 AGYVPCNILA EARQEDVGAP LEQSGQKYWG PASPTHKLPP IFAGNKEELI HHMDEVNDEL 610 620 630 640 650 660 MKKISHIKTQ PQRNFRVERS QPVHLPLTFE SGPDEVRAWL EAKAFSARIV ENLGILTGPQ 670 680 690 700 710 720 LFSLNKEELK KVCGEEGSRV YSQLTVQKAF LEKQQSGSEL EKLMSKIRRA EDSYTSQHTS PESEGAPHL
	Isoform 2 [UniParc]. Checksum: DE7B8FA22B454B20	FASTA	400	44,740
	10 20 30 40 50 60 MSQSASMSCC PGAANGSLGR SDGVPRMSAK DLFEQRKKYS NSNVIMHETS QYHVQHLATF 70 80 90 100 110 120 IMDKSEAIAS VDDAIRKLVQ LSSKEKVWAQ EVLLQVNDKS LRLLDVESQE ELENFPLPTV 130 140 150 160 170 180 QHSQTVLNQL RYPSVLLLVC QDSDQNKPDI HFFHCDEVEA ELVQEDIESA LADYRLGKKM 190 200 210 220 230 240 RPQTLKGHQE KIRQRQSILP PPQSPAPIPF QRQPGDSPQA KNRVGLPLPV PFSEPGYRRR 250 260 270 280 290 300 ESQDEEPRAV LAQRIEKETQ ILNCTLDDIE WFVARLQKAA EAFKQLNQRK KGKKKNKKGP 310 320 330 340 350 360 AEGVLTLRAR PPSEGEFVDC FQKTKLAINL LAKLQKHIQN PSAAELVHFL FGPLDLVTGA 370 380 390 400 GTGRGRGQQS DPHGFGVEET SAHLSILKLC LGSQEATVHP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J and FVB/N. Tissue: Mammary tumor.
[2]	"The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway." Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G. Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[3]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-242 AND SER-483, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC005492 mRNA. Translation: AAH05492.1. BC009098 mRNA. Translation: AAH09098.1.
IPI	IPI00115492. IPI00759947.
RefSeq	NP_573454.2. NM_133191.2.
UniGene	Mm.27451.
3D structure databases
ProteinModelPortal	Q99K30.
SMR	Q99K30. Positions 46-175, 498-552, 615-700.
ModBase	Search...
PTM databases
PhosphoSite	Q99K30.
Proteomic databases
PaxDb	Q99K30.
PRIDE	Q99K30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026577; ENSMUSP00000026577; ENSMUSG00000025504.
GeneID	98845.
KEGG	mmu:98845.
UCSC	uc009kkt.1. mouse.
Organism-specific databases
CTD	64787.
MGI	MGI:2138828. Eps8l2.
Phylogenomic databases
eggNOG	NOG263108.
GeneTree	ENSGT00390000003646.
HOGENOM	HOG000060324.
HOVERGEN	HBG003090.
InParanoid	Q99K30.
OMA	QKYWGPA.
OrthoDB	EOG4X0MRS.
Gene expression databases
ArrayExpress	Q99K30.
Bgee	Q99K30.
CleanEx	MM_EPS8L2.
Genevestigator	Q99K30.
GermOnline	ENSMUSG00000025504. Mus musculus.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR013625. PTB. IPR006020. PTyr_interaction_dom. IPR001452. SH3_domain. [Graphical view]
Pfam	PF08416. PTB. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS01179. PID. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	EPS8L2. mouse.
NextBio	353685.
SOURCE	Search...

Entry information

Entry name

ES8L2_MOUSE

Accession

Primary (citable) accession number: Q99K30
Secondary accession number(s): Q91VT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program