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Q99K30 (ES8L2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 2

Short name=EPS8-like protein 2
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 2
Short name=EPS8-related protein 2
Gene names
Name:Eps8l2
Synonyms:Eps8r2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton By similarity.

Subunit structure

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Detected in fetal kidney, adrenal gland, salivary gland, stomach, gut, cartilage and skin. Detected in adult ovary, placenta, skin, adrenal gland, salivary gland, kidney, intestine and stomach. Ref.2

Sequence similarities

Belongs to the EPS8 family.

Contains 1 PID domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   DomainSH3 domain
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRac protein signal transduction

Inferred from sequence orthology Ref.2. Source: MGI

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: Ensembl

ruffle

Inferred from sequence orthology Ref.2. Source: MGI

   Molecular_functionactin filament binding

Inferred from sequence orthology Ref.2. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99K30-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99K30-2)

The sequence of this isoform differs from the canonical sequence as follows:
     357-400: IINTCGSPDI...EMTLWESLGE → VTGAGTGRGR...LGSQEATVHP
     401-729: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729Epidermal growth factor receptor kinase substrate 8-like protein 2
PRO_0000239085

Regions

Domain46 – 202157PID
Domain495 – 55460SH3

Amino acid modifications

Modified residue3061Phosphothreonine Ref.3
Modified residue5731Phosphoserine By similarity

Natural variations

Alternative sequence357 – 40044IINTC…ESLGE → VTGAGTGRGRGQQSDPHGFG VEETSAHLSILKLCLGSQEA TVHP in isoform 2.
VSP_019093
Alternative sequence401 – 729329Missing in isoform 2.
VSP_019094

Experimental info

Sequence conflict1771G → R in AAH09098. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: A11DE82FF2C0BD18

FASTA72982,229
        10         20         30         40         50         60 
MSQSASMSCC PGAANGSLGR SDGVPRMSAK DLFEQRKKYS NSNVIMHETS QYHVQHLATF 

        70         80         90        100        110        120 
IMDKSEAIAS VDDAIRKLVQ LSSKEKVWAQ EVLLQVNDKS LRLLDVESQE ELENFPLPTV 

       130        140        150        160        170        180 
QHSQTVLNQL RYPSVLLLVC QDSDQNKPDI HFFHCDEVEA ELVQEDIESA LADYRLGKKM 

       190        200        210        220        230        240 
RPQTLKGHQE KIRQRQSILP PPQSPAPIPF QRQPGDSPQA KNRVGLPLPV PFSEPGYRRR 

       250        260        270        280        290        300 
ESQDEEPRAV LAQRIEKETQ ILNCTLDDIE WFVARLQKAA EAFKQLNQRK KGKKKNKKGP 

       310        320        330        340        350        360 
AEGVLTLRAR PPSEGEFVDC FQKTKLAINL LAKLQKHIQN PSAAELVHFL FGPLDLIINT 

       370        380        390        400        410        420 
CGSPDIARSV SSPLLSTDAV SFLRGHLVPK EMTLWESLGE TWMRPRSEWP REPQVPLYVP 

       430        440        450        460        470        480 
KFRSGWEPPL DVLQEAPWEV EGLASVPSDQ LTPKNRLSVR HSPKHSLSSE SQAPEDIAPP 

       490        500        510        520        530        540 
GSSPHANRGY QPTPAMTKYV KILYDFTARN ANELSVLKDE VLEVLEDGRQ WWKLRNRSGQ 

       550        560        570        580        590        600 
AGYVPCNILA EARQEDVGAP LEQSGQKYWG PASPTHKLPP IFAGNKEELI HHMDEVNDEL 

       610        620        630        640        650        660 
MKKISHIKTQ PQRNFRVERS QPVHLPLTFE SGPDEVRAWL EAKAFSARIV ENLGILTGPQ 

       670        680        690        700        710        720 
LFSLNKEELK KVCGEEGSRV YSQLTVQKAF LEKQQSGSEL EKLMSKIRRA EDSYTSQHTS 


PESEGAPHL 

« Hide

Isoform 2 [UniParc].

Checksum: DE7B8FA22B454B20
Show »

FASTA40044,740

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J and FVB/N.
Tissue: Mammary tumor.
[2]"The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway."
Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G.
Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC005492 mRNA. Translation: AAH05492.1.
BC009098 mRNA. Translation: AAH09098.1.
RefSeqNP_573454.2. NM_133191.2.
UniGeneMm.27451.

3D structure databases

ProteinModelPortalQ99K30.
SMRQ99K30. Positions 46-175, 498-552, 615-700.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ99K30. 2 interactions.
MINTMINT-1860812.

PTM databases

PhosphoSiteQ99K30.

Proteomic databases

PaxDbQ99K30.
PRIDEQ99K30.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026577; ENSMUSP00000026577; ENSMUSG00000025504. [Q99K30-1]
GeneID98845.
KEGGmmu:98845.
UCSCuc009kkt.1. mouse. [Q99K30-1]

Organism-specific databases

CTD64787.
MGIMGI:2138828. Eps8l2.

Phylogenomic databases

eggNOGNOG263108.
GeneTreeENSGT00390000003646.
HOGENOMHOG000060324.
HOVERGENHBG003090.
InParanoidQ99K30.
KOK17277.
OMAVLQEAPW.
OrthoDBEOG7TMZR9.
PhylomeDBQ99K30.
TreeFamTF313069.

Gene expression databases

ArrayExpressQ99K30.
BgeeQ99K30.
CleanExMM_EPS8L2.
GenevestigatorQ99K30.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSEPS8L2. mouse.
NextBio353685.
PROQ99K30.
SOURCESearch...

Entry information

Entry nameES8L2_MOUSE
AccessionPrimary (citable) accession number: Q99K30
Secondary accession number(s): Q91VT7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot