Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q99K30

- ES8L2_MOUSE

UniProt

Q99K30 - ES8L2_MOUSE

Protein

Epidermal growth factor receptor kinase substrate 8-like protein 2

Gene

Eps8l2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton By similarity.By similarity

    GO - Molecular functioni

    1. actin filament binding Source: MGI

    GO - Biological processi

    1. Rac protein signal transduction Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epidermal growth factor receptor kinase substrate 8-like protein 2
    Short name:
    EPS8-like protein 2
    Alternative name(s):
    Epidermal growth factor receptor pathway substrate 8-related protein 2
    Short name:
    EPS8-related protein 2
    Gene namesi
    Name:Eps8l2
    Synonyms:Eps8r2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:2138828. Eps8l2.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: Ensembl
    3. ruffle Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 729729Epidermal growth factor receptor kinase substrate 8-like protein 2PRO_0000239085Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei306 – 3061Phosphothreonine1 Publication
    Modified residuei573 – 5731PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ99K30.
    PaxDbiQ99K30.
    PRIDEiQ99K30.

    PTM databases

    PhosphoSiteiQ99K30.

    Expressioni

    Tissue specificityi

    Detected in fetal kidney, adrenal gland, salivary gland, stomach, gut, cartilage and skin. Detected in adult ovary, placenta, skin, adrenal gland, salivary gland, kidney, intestine and stomach.1 Publication

    Gene expression databases

    ArrayExpressiQ99K30.
    BgeeiQ99K30.
    CleanExiMM_EPS8L2.
    GenevestigatoriQ99K30.

    Interactioni

    Subunit structurei

    Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin By similarity.By similarity

    Protein-protein interaction databases

    BioGridi221147. 1 interaction.
    IntActiQ99K30. 2 interactions.
    MINTiMINT-1860812.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99K30.
    SMRiQ99K30. Positions 46-175, 498-552, 615-700.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini46 – 202157PIDPROSITE-ProRule annotationAdd
    BLAST
    Domaini495 – 55460SH3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the EPS8 family.Curated
    Contains 1 PID domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiNOG263108.
    GeneTreeiENSGT00390000003646.
    HOGENOMiHOG000060324.
    HOVERGENiHBG003090.
    InParanoidiQ99K30.
    KOiK17277.
    OMAiVLQEAPW.
    OrthoDBiEOG7TMZR9.
    PhylomeDBiQ99K30.
    TreeFamiTF313069.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR011993. PH_like_dom.
    IPR013625. PTB.
    IPR006020. PTB/PI_dom.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF08416. PTB. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS01179. PID. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99K30-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSQSASMSCC PGAANGSLGR SDGVPRMSAK DLFEQRKKYS NSNVIMHETS    50
    QYHVQHLATF IMDKSEAIAS VDDAIRKLVQ LSSKEKVWAQ EVLLQVNDKS 100
    LRLLDVESQE ELENFPLPTV QHSQTVLNQL RYPSVLLLVC QDSDQNKPDI 150
    HFFHCDEVEA ELVQEDIESA LADYRLGKKM RPQTLKGHQE KIRQRQSILP 200
    PPQSPAPIPF QRQPGDSPQA KNRVGLPLPV PFSEPGYRRR ESQDEEPRAV 250
    LAQRIEKETQ ILNCTLDDIE WFVARLQKAA EAFKQLNQRK KGKKKNKKGP 300
    AEGVLTLRAR PPSEGEFVDC FQKTKLAINL LAKLQKHIQN PSAAELVHFL 350
    FGPLDLIINT CGSPDIARSV SSPLLSTDAV SFLRGHLVPK EMTLWESLGE 400
    TWMRPRSEWP REPQVPLYVP KFRSGWEPPL DVLQEAPWEV EGLASVPSDQ 450
    LTPKNRLSVR HSPKHSLSSE SQAPEDIAPP GSSPHANRGY QPTPAMTKYV 500
    KILYDFTARN ANELSVLKDE VLEVLEDGRQ WWKLRNRSGQ AGYVPCNILA 550
    EARQEDVGAP LEQSGQKYWG PASPTHKLPP IFAGNKEELI HHMDEVNDEL 600
    MKKISHIKTQ PQRNFRVERS QPVHLPLTFE SGPDEVRAWL EAKAFSARIV 650
    ENLGILTGPQ LFSLNKEELK KVCGEEGSRV YSQLTVQKAF LEKQQSGSEL 700
    EKLMSKIRRA EDSYTSQHTS PESEGAPHL 729
    Length:729
    Mass (Da):82,229
    Last modified:June 1, 2001 - v1
    Checksum:iA11DE82FF2C0BD18
    GO
    Isoform 2 (identifier: Q99K30-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         357-400: IINTCGSPDI...EMTLWESLGE → VTGAGTGRGR...LGSQEATVHP
         401-729: Missing.

    Show »
    Length:400
    Mass (Da):44,740
    Checksum:iDE7B8FA22B454B20
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti177 – 1771G → R in AAH09098. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei357 – 40044IINTC…ESLGE → VTGAGTGRGRGQQSDPHGFG VEETSAHLSILKLCLGSQEA TVHP in isoform 2. 1 PublicationVSP_019093Add
    BLAST
    Alternative sequencei401 – 729329Missing in isoform 2. 1 PublicationVSP_019094Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC005492 mRNA. Translation: AAH05492.1.
    BC009098 mRNA. Translation: AAH09098.1.
    CCDSiCCDS22009.1. [Q99K30-1]
    RefSeqiNP_573454.2. NM_133191.2. [Q99K30-1]
    UniGeneiMm.27451.

    Genome annotation databases

    EnsembliENSMUST00000026577; ENSMUSP00000026577; ENSMUSG00000025504. [Q99K30-1]
    GeneIDi98845.
    KEGGimmu:98845.
    UCSCiuc009kkt.1. mouse. [Q99K30-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC005492 mRNA. Translation: AAH05492.1 .
    BC009098 mRNA. Translation: AAH09098.1 .
    CCDSi CCDS22009.1. [Q99K30-1 ]
    RefSeqi NP_573454.2. NM_133191.2. [Q99K30-1 ]
    UniGenei Mm.27451.

    3D structure databases

    ProteinModelPortali Q99K30.
    SMRi Q99K30. Positions 46-175, 498-552, 615-700.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 221147. 1 interaction.
    IntActi Q99K30. 2 interactions.
    MINTi MINT-1860812.

    PTM databases

    PhosphoSitei Q99K30.

    Proteomic databases

    MaxQBi Q99K30.
    PaxDbi Q99K30.
    PRIDEi Q99K30.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026577 ; ENSMUSP00000026577 ; ENSMUSG00000025504 . [Q99K30-1 ]
    GeneIDi 98845.
    KEGGi mmu:98845.
    UCSCi uc009kkt.1. mouse. [Q99K30-1 ]

    Organism-specific databases

    CTDi 64787.
    MGIi MGI:2138828. Eps8l2.

    Phylogenomic databases

    eggNOGi NOG263108.
    GeneTreei ENSGT00390000003646.
    HOGENOMi HOG000060324.
    HOVERGENi HBG003090.
    InParanoidi Q99K30.
    KOi K17277.
    OMAi VLQEAPW.
    OrthoDBi EOG7TMZR9.
    PhylomeDBi Q99K30.
    TreeFami TF313069.

    Miscellaneous databases

    ChiTaRSi EPS8L2. mouse.
    NextBioi 353685.
    PROi Q99K30.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99K30.
    Bgeei Q99K30.
    CleanExi MM_EPS8L2.
    Genevestigatori Q99K30.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR011993. PH_like_dom.
    IPR013625. PTB.
    IPR006020. PTB/PI_dom.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF08416. PTB. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS01179. PID. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J and FVB/N.
      Tissue: Mammary tumor.
    2. "The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway."
      Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G.
      Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiES8L2_MOUSE
    AccessioniPrimary (citable) accession number: Q99K30
    Secondary accession number(s): Q91VT7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3