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Protein

Epidermal growth factor receptor kinase substrate 8-like protein 2

Gene

Eps8l2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton (By similarity).By similarity

GO - Molecular functioni

  1. actin binding Source: MGI
  2. actin filament binding Source: MGI
  3. Rac guanyl-nucleotide exchange factor activity Source: Ensembl

GO - Biological processi

  1. positive regulation of Rac GTPase activity Source: MGI
  2. positive regulation of Rho GTPase activity Source: MGI
  3. positive regulation of ruffle assembly Source: MGI
  4. Rac protein signal transduction Source: MGI
  5. regulation of Rho protein signal transduction Source: MGI
  6. Rho protein signal transduction Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 2
Short name:
EPS8-like protein 2
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 2
Short name:
EPS8-related protein 2
Gene namesi
Name:Eps8l2
Synonyms:Eps8r2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2138828. Eps8l2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular vesicular exosome Source: MGI
  3. plasma membrane Source: MGI
  4. protein complex Source: MGI
  5. ruffle Source: MGI
  6. ruffle membrane Source: MGI
  7. vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Epidermal growth factor receptor kinase substrate 8-like protein 2PRO_0000239085Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei242 – 2421PhosphoserineBy similarity
Modified residuei306 – 3061Phosphothreonine1 Publication
Modified residuei573 – 5731PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99K30.
PaxDbiQ99K30.
PRIDEiQ99K30.

PTM databases

PhosphoSiteiQ99K30.

Expressioni

Tissue specificityi

Detected in fetal kidney, adrenal gland, salivary gland, stomach, gut, cartilage and skin. Detected in adult ovary, placenta, skin, adrenal gland, salivary gland, kidney, intestine and stomach.1 Publication

Gene expression databases

BgeeiQ99K30.
CleanExiMM_EPS8L2.
ExpressionAtlasiQ99K30. baseline and differential.
GenevestigatoriQ99K30.

Interactioni

Subunit structurei

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin (By similarity).By similarity

Protein-protein interaction databases

BioGridi221147. 1 interaction.
IntActiQ99K30. 2 interactions.
MINTiMINT-1860812.

Structurei

3D structure databases

ProteinModelPortaliQ99K30.
SMRiQ99K30. Positions 46-175, 498-552, 615-700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 202157PIDPROSITE-ProRule annotationAdd
BLAST
Domaini495 – 55460SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the EPS8 family.Curated
Contains 1 PID domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG263108.
GeneTreeiENSGT00390000003646.
HOGENOMiHOG000060324.
HOVERGENiHBG003090.
InParanoidiQ99K30.
KOiK17277.
OMAiERSQPVH.
OrthoDBiEOG7TMZR9.
PhylomeDBiQ99K30.
TreeFamiTF313069.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_like_dom.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99K30-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQSASMSCC PGAANGSLGR SDGVPRMSAK DLFEQRKKYS NSNVIMHETS
60 70 80 90 100
QYHVQHLATF IMDKSEAIAS VDDAIRKLVQ LSSKEKVWAQ EVLLQVNDKS
110 120 130 140 150
LRLLDVESQE ELENFPLPTV QHSQTVLNQL RYPSVLLLVC QDSDQNKPDI
160 170 180 190 200
HFFHCDEVEA ELVQEDIESA LADYRLGKKM RPQTLKGHQE KIRQRQSILP
210 220 230 240 250
PPQSPAPIPF QRQPGDSPQA KNRVGLPLPV PFSEPGYRRR ESQDEEPRAV
260 270 280 290 300
LAQRIEKETQ ILNCTLDDIE WFVARLQKAA EAFKQLNQRK KGKKKNKKGP
310 320 330 340 350
AEGVLTLRAR PPSEGEFVDC FQKTKLAINL LAKLQKHIQN PSAAELVHFL
360 370 380 390 400
FGPLDLIINT CGSPDIARSV SSPLLSTDAV SFLRGHLVPK EMTLWESLGE
410 420 430 440 450
TWMRPRSEWP REPQVPLYVP KFRSGWEPPL DVLQEAPWEV EGLASVPSDQ
460 470 480 490 500
LTPKNRLSVR HSPKHSLSSE SQAPEDIAPP GSSPHANRGY QPTPAMTKYV
510 520 530 540 550
KILYDFTARN ANELSVLKDE VLEVLEDGRQ WWKLRNRSGQ AGYVPCNILA
560 570 580 590 600
EARQEDVGAP LEQSGQKYWG PASPTHKLPP IFAGNKEELI HHMDEVNDEL
610 620 630 640 650
MKKISHIKTQ PQRNFRVERS QPVHLPLTFE SGPDEVRAWL EAKAFSARIV
660 670 680 690 700
ENLGILTGPQ LFSLNKEELK KVCGEEGSRV YSQLTVQKAF LEKQQSGSEL
710 720
EKLMSKIRRA EDSYTSQHTS PESEGAPHL
Length:729
Mass (Da):82,229
Last modified:May 31, 2001 - v1
Checksum:iA11DE82FF2C0BD18
GO
Isoform 2 (identifier: Q99K30-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     357-400: IINTCGSPDI...EMTLWESLGE → VTGAGTGRGR...LGSQEATVHP
     401-729: Missing.

Show »
Length:400
Mass (Da):44,740
Checksum:iDE7B8FA22B454B20
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti177 – 1771G → R in AAH09098 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei357 – 40044IINTC…ESLGE → VTGAGTGRGRGQQSDPHGFG VEETSAHLSILKLCLGSQEA TVHP in isoform 2. 1 PublicationVSP_019093Add
BLAST
Alternative sequencei401 – 729329Missing in isoform 2. 1 PublicationVSP_019094Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC005492 mRNA. Translation: AAH05492.1.
BC009098 mRNA. Translation: AAH09098.1.
CCDSiCCDS22009.1. [Q99K30-1]
RefSeqiNP_573454.2. NM_133191.2. [Q99K30-1]
UniGeneiMm.27451.

Genome annotation databases

EnsembliENSMUST00000026577; ENSMUSP00000026577; ENSMUSG00000025504. [Q99K30-1]
GeneIDi98845.
KEGGimmu:98845.
UCSCiuc009kkt.1. mouse. [Q99K30-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC005492 mRNA. Translation: AAH05492.1.
BC009098 mRNA. Translation: AAH09098.1.
CCDSiCCDS22009.1. [Q99K30-1]
RefSeqiNP_573454.2. NM_133191.2. [Q99K30-1]
UniGeneiMm.27451.

3D structure databases

ProteinModelPortaliQ99K30.
SMRiQ99K30. Positions 46-175, 498-552, 615-700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221147. 1 interaction.
IntActiQ99K30. 2 interactions.
MINTiMINT-1860812.

PTM databases

PhosphoSiteiQ99K30.

Proteomic databases

MaxQBiQ99K30.
PaxDbiQ99K30.
PRIDEiQ99K30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026577; ENSMUSP00000026577; ENSMUSG00000025504. [Q99K30-1]
GeneIDi98845.
KEGGimmu:98845.
UCSCiuc009kkt.1. mouse. [Q99K30-1]

Organism-specific databases

CTDi64787.
MGIiMGI:2138828. Eps8l2.

Phylogenomic databases

eggNOGiNOG263108.
GeneTreeiENSGT00390000003646.
HOGENOMiHOG000060324.
HOVERGENiHBG003090.
InParanoidiQ99K30.
KOiK17277.
OMAiERSQPVH.
OrthoDBiEOG7TMZR9.
PhylomeDBiQ99K30.
TreeFamiTF313069.

Miscellaneous databases

NextBioi353685.
PROiQ99K30.
SOURCEiSearch...

Gene expression databases

BgeeiQ99K30.
CleanExiMM_EPS8L2.
ExpressionAtlasiQ99K30. baseline and differential.
GenevestigatoriQ99K30.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_like_dom.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and FVB/N.
    Tissue: Mammary tumor.
  2. "The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway."
    Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G.
    Mol. Biol. Cell 15:91-98(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiES8L2_MOUSE
AccessioniPrimary (citable) accession number: Q99K30
Secondary accession number(s): Q91VT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2006
Last sequence update: May 31, 2001
Last modified: March 3, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.