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Q99K30

- ES8L2_MOUSE

UniProt

Q99K30 - ES8L2_MOUSE

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Protein
Epidermal growth factor receptor kinase substrate 8-like protein 2
Gene
Eps8l2, Eps8r2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton By similarity.

GO - Molecular functioni

  1. actin filament binding Source: MGI
Complete GO annotation...

GO - Biological processi

  1. Rac protein signal transduction Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 2
Short name:
EPS8-like protein 2
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 2
Short name:
EPS8-related protein 2
Gene namesi
Name:Eps8l2
Synonyms:Eps8r2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2138828. Eps8l2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: Ensembl
  3. ruffle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Epidermal growth factor receptor kinase substrate 8-like protein 2
PRO_0000239085Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei306 – 3061Phosphothreonine1 Publication
Modified residuei573 – 5731Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99K30.
PaxDbiQ99K30.
PRIDEiQ99K30.

PTM databases

PhosphoSiteiQ99K30.

Expressioni

Tissue specificityi

Detected in fetal kidney, adrenal gland, salivary gland, stomach, gut, cartilage and skin. Detected in adult ovary, placenta, skin, adrenal gland, salivary gland, kidney, intestine and stomach.1 Publication

Gene expression databases

ArrayExpressiQ99K30.
BgeeiQ99K30.
CleanExiMM_EPS8L2.
GenevestigatoriQ99K30.

Interactioni

Subunit structurei

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin By similarity.

Protein-protein interaction databases

BioGridi221147. 1 interaction.
IntActiQ99K30. 2 interactions.
MINTiMINT-1860812.

Structurei

3D structure databases

ProteinModelPortaliQ99K30.
SMRiQ99K30. Positions 46-175, 498-552, 615-700.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 202157PID
Add
BLAST
Domaini495 – 55460SH3
Add
BLAST

Sequence similaritiesi

Belongs to the EPS8 family.
Contains 1 PID domain.
Contains 1 SH3 domain.

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG263108.
GeneTreeiENSGT00390000003646.
HOGENOMiHOG000060324.
HOVERGENiHBG003090.
InParanoidiQ99K30.
KOiK17277.
OMAiVLQEAPW.
OrthoDBiEOG7TMZR9.
PhylomeDBiQ99K30.
TreeFamiTF313069.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_like_dom.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q99K30-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSQSASMSCC PGAANGSLGR SDGVPRMSAK DLFEQRKKYS NSNVIMHETS    50
QYHVQHLATF IMDKSEAIAS VDDAIRKLVQ LSSKEKVWAQ EVLLQVNDKS 100
LRLLDVESQE ELENFPLPTV QHSQTVLNQL RYPSVLLLVC QDSDQNKPDI 150
HFFHCDEVEA ELVQEDIESA LADYRLGKKM RPQTLKGHQE KIRQRQSILP 200
PPQSPAPIPF QRQPGDSPQA KNRVGLPLPV PFSEPGYRRR ESQDEEPRAV 250
LAQRIEKETQ ILNCTLDDIE WFVARLQKAA EAFKQLNQRK KGKKKNKKGP 300
AEGVLTLRAR PPSEGEFVDC FQKTKLAINL LAKLQKHIQN PSAAELVHFL 350
FGPLDLIINT CGSPDIARSV SSPLLSTDAV SFLRGHLVPK EMTLWESLGE 400
TWMRPRSEWP REPQVPLYVP KFRSGWEPPL DVLQEAPWEV EGLASVPSDQ 450
LTPKNRLSVR HSPKHSLSSE SQAPEDIAPP GSSPHANRGY QPTPAMTKYV 500
KILYDFTARN ANELSVLKDE VLEVLEDGRQ WWKLRNRSGQ AGYVPCNILA 550
EARQEDVGAP LEQSGQKYWG PASPTHKLPP IFAGNKEELI HHMDEVNDEL 600
MKKISHIKTQ PQRNFRVERS QPVHLPLTFE SGPDEVRAWL EAKAFSARIV 650
ENLGILTGPQ LFSLNKEELK KVCGEEGSRV YSQLTVQKAF LEKQQSGSEL 700
EKLMSKIRRA EDSYTSQHTS PESEGAPHL 729
Length:729
Mass (Da):82,229
Last modified:June 1, 2001 - v1
Checksum:iA11DE82FF2C0BD18
GO
Isoform 2 (identifier: Q99K30-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     357-400: IINTCGSPDI...EMTLWESLGE → VTGAGTGRGR...LGSQEATVHP
     401-729: Missing.

Show »
Length:400
Mass (Da):44,740
Checksum:iDE7B8FA22B454B20
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei357 – 40044IINTC…ESLGE → VTGAGTGRGRGQQSDPHGFG VEETSAHLSILKLCLGSQEA TVHP in isoform 2.
VSP_019093Add
BLAST
Alternative sequencei401 – 729329Missing in isoform 2.
VSP_019094Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti177 – 1771G → R in AAH09098. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC005492 mRNA. Translation: AAH05492.1.
BC009098 mRNA. Translation: AAH09098.1.
CCDSiCCDS22009.1. [Q99K30-1]
RefSeqiNP_573454.2. NM_133191.2. [Q99K30-1]
UniGeneiMm.27451.

Genome annotation databases

EnsembliENSMUST00000026577; ENSMUSP00000026577; ENSMUSG00000025504. [Q99K30-1]
GeneIDi98845.
KEGGimmu:98845.
UCSCiuc009kkt.1. mouse. [Q99K30-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC005492 mRNA. Translation: AAH05492.1 .
BC009098 mRNA. Translation: AAH09098.1 .
CCDSi CCDS22009.1. [Q99K30-1 ]
RefSeqi NP_573454.2. NM_133191.2. [Q99K30-1 ]
UniGenei Mm.27451.

3D structure databases

ProteinModelPortali Q99K30.
SMRi Q99K30. Positions 46-175, 498-552, 615-700.
ModBasei Search...

Protein-protein interaction databases

BioGridi 221147. 1 interaction.
IntActi Q99K30. 2 interactions.
MINTi MINT-1860812.

PTM databases

PhosphoSitei Q99K30.

Proteomic databases

MaxQBi Q99K30.
PaxDbi Q99K30.
PRIDEi Q99K30.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026577 ; ENSMUSP00000026577 ; ENSMUSG00000025504 . [Q99K30-1 ]
GeneIDi 98845.
KEGGi mmu:98845.
UCSCi uc009kkt.1. mouse. [Q99K30-1 ]

Organism-specific databases

CTDi 64787.
MGIi MGI:2138828. Eps8l2.

Phylogenomic databases

eggNOGi NOG263108.
GeneTreei ENSGT00390000003646.
HOGENOMi HOG000060324.
HOVERGENi HBG003090.
InParanoidi Q99K30.
KOi K17277.
OMAi VLQEAPW.
OrthoDBi EOG7TMZR9.
PhylomeDBi Q99K30.
TreeFami TF313069.

Miscellaneous databases

ChiTaRSi EPS8L2. mouse.
NextBioi 353685.
PROi Q99K30.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q99K30.
Bgeei Q99K30.
CleanExi MM_EPS8L2.
Genevestigatori Q99K30.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR011993. PH_like_dom.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
SMARTi SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and FVB/N.
    Tissue: Mammary tumor.
  2. "The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway."
    Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G.
    Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiES8L2_MOUSE
AccessioniPrimary (citable) accession number: Q99K30
Secondary accession number(s): Q91VT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi