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Protein

ADP-ribosylation factor GTPase-activating protein 2

Gene

Arfgap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). May regulate coatomer-mediated protein transport from the Golgi complex to the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri26 – 49C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
Biological processER-Golgi transport, Protein transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor GTPase-activating protein 2
Short name:
ARF GAP 2
Alternative name(s):
GTPase-activating protein ZNF289
Zinc finger protein 289
Gene namesi
Name:Arfgap2
Synonyms:Zfp289, Znf289
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1924288 Arfgap2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002784692 – 520ADP-ribosylation factor GTPase-activating protein 2Add BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei140PhosphoserineBy similarity1
Modified residuei145PhosphoserineCombined sources1
Modified residuei200PhosphoserineBy similarity1
Modified residuei236PhosphoserineBy similarity1
Modified residuei239PhosphoserineCombined sources1
Modified residuei311PhosphoserineBy similarity1
Modified residuei333PhosphoserineBy similarity1
Modified residuei339PhosphoserineCombined sources1
Modified residuei363PhosphoserineBy similarity1
Modified residuei431PhosphoserineBy similarity1
Modified residuei432PhosphoserineBy similarity1
Modified residuei512PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99K28
PaxDbiQ99K28
PeptideAtlasiQ99K28
PRIDEiQ99K28

PTM databases

iPTMnetiQ99K28
PhosphoSitePlusiQ99K28

Expressioni

Tissue specificityi

Highly expressed in liver, heart and kidney. Low expression in skeletal muscle and spleen.1 Publication

Developmental stagei

High levels in mammary glands of virgin mice and in mammary glands of pregnant mice associated with extensive proliferation of ductal cells and lobulo-alveolar development. Expression declines at the beginning of lactation when the glands fully differentiate. No expression after day 2 of lactation until day 21. Expression may be controlled by ID1.1 Publication

Gene expression databases

BgeeiENSMUSG00000027255
GenevisibleiQ99K28 MM

Interactioni

Subunit structurei

Interacts with the coatomer complex. Interacts with the C-terminal appendage domain of COPG1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi218475, 1 interactor
IntActiQ99K28, 1 interactor
MINTiQ99K28
STRINGi10090.ENSMUSP00000028691

Structurei

3D structure databases

ProteinModelPortaliQ99K28
SMRiQ99K28
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 127Arf-GAPPROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 520Required for interaction with coatomerBy similarityAdd BLAST424

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili241 – 307Sequence analysisAdd BLAST67

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri26 – 49C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0706 Eukaryota
COG5347 LUCA
GeneTreeiENSGT00390000005436
HOGENOMiHOG000162970
HOVERGENiHBG050563
InParanoidiQ99K28
KOiK12493
OMAiDQWQWDQ
OrthoDBiEOG091G0AS9
PhylomeDBiQ99K28
TreeFamiTF313985

Family and domain databases

Gene3Di3.30.40.160, 1 hit
InterProiView protein in InterPro
IPR037278 ARFGAP/RecO
IPR001164 ArfGAP_dom
IPR038508 ArfGAP_dom_sf
PfamiView protein in Pfam
PF01412 ArfGap, 1 hit
PRINTSiPR00405 REVINTRACTNG
SMARTiView protein in SMART
SM00105 ArfGap, 1 hit
SUPFAMiSSF57863 SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50115 ARFGAP, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99K28-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASPSKTEI QTIFKRLRAI PTNKACFDCG AKSPSWASIT YGVFLCIDCS
60 70 80 90 100
GVHRSLGVHL SFIRSTELDS NWSWLQLRCM QVGGNANATA FFRQHGCMAN
110 120 130 140 150
DANTKYTSRA AQMYREKIRQ LGSAALTRHG TDLWIDSMNS APSHSPEKKD
160 170 180 190 200
SDFFTEHTQA PAWDTAATDP SGTQQPALPS ESSSLAQPEQ GPNTDLLGTS
210 220 230 240 250
PQASLELKSS IIGKKKPAAA KKGLGAKKGL GAQKVSNQSF TEIERQAQVA
260 270 280 290 300
EKLREQQAAD AKKQAEESMV ASMRLAYQEL QIDRKKEEKK LQNLEGKKRE
310 320 330 340 350
QAERLGMGLV SRSSISHSVL SEMQMIEQET PLSAKSSRSQ LDLFDDVGTF
360 370 380 390 400
ASGPPKYKDN PFSLGETFGS RWDSDAAWGM DRVEEKEPEV TISSIRPISE
410 420 430 440 450
RTASRREVET RSSGLESSEA RQKFAGAKAI SSDMFFGREV DSEYEARSRL
460 470 480 490 500
QQLSGSSAIS SSDLFGNMDG AHGGGTVSLG NVLPTADIAQ FKQGVKSVAG
510 520
KMAVLANGVM NSLQDRYGSY
Length:520
Mass (Da):56,598
Last modified:June 1, 2001 - v1
Checksum:i9B06038602F15014
GO
Isoform 2 (identifier: Q99K28-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     206-206: E → ESMYLSAKGPSCTRE

Note: No experimental confirmation available.
Show »
Length:534
Mass (Da):58,110
Checksum:i5711CBFABECB1ECA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti467N → D in AAF91258 (PubMed:11278321).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023305206E → ESMYLSAKGPSCTRE in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF229439 mRNA Translation: AAF91258.1
AK009565 mRNA Translation: BAB26362.1
AK168489 mRNA Translation: BAE40376.1
AK169144 mRNA Translation: BAE40923.1
AK149837 mRNA Translation: BAE29114.1
AL732478 Genomic DNA No translation available.
BC005495 mRNA Translation: AAH05495.1
CCDSiCCDS16430.1 [Q99K28-1]
CCDS50639.1 [Q99K28-2]
RefSeqiNP_001159496.1, NM_001166024.1 [Q99K28-2]
NP_076343.2, NM_023854.2 [Q99K28-1]
UniGeneiMm.386861
Mm.43636

Genome annotation databases

EnsembliENSMUST00000028691; ENSMUSP00000028691; ENSMUSG00000027255 [Q99K28-2]
ENSMUST00000080008; ENSMUSP00000078920; ENSMUSG00000027255 [Q99K28-1]
GeneIDi77038
KEGGimmu:77038
UCSCiuc008kvq.2 mouse [Q99K28-1]
uc008kvr.2 mouse [Q99K28-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiARFG2_MOUSE
AccessioniPrimary (citable) accession number: Q99K28
Secondary accession number(s): Q9D758, Q9JIH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health