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Protein

Phospholipid phosphatase 3

Gene

Plpp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P) (By similarity). Essential to the formation of the chorioallantoic placenta and extraembryonic vasculature. Also mediates gastrulation and axis formation, probably by regulating the Wnt signaling pathway.By similarity1 Publication

Catalytic activityi

A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.

GO - Molecular functioni

  • integrin binding Source: MGI
  • lipid phosphatase activity Source: MGI
  • phosphatidate phosphatase activity Source: UniProtKB-EC
  • sphingosine-1-phosphate phosphatase activity Source: MGI

GO - Biological processi

  • Bergmann glial cell differentiation Source: MGI
  • blood vessel development Source: MGI
  • canonical Wnt signaling pathway Source: BHF-UCL
  • canonical Wnt signaling pathway involved in positive regulation of cell-cell adhesion Source: MGI
  • canonical Wnt signaling pathway involved in positive regulation of endothelial cell migration Source: MGI
  • canonical Wnt signaling pathway involved in positive regulation of wound healing Source: MGI
  • cell adhesion Source: MGI
  • gastrulation with mouth forming second Source: MGI
  • homotypic cell-cell adhesion Source: MGI
  • negative regulation of protein phosphorylation Source: BHF-UCL
  • phospholipid dephosphorylation Source: GO_Central
  • phospholipid metabolic process Source: MGI
  • positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  • protein stabilization Source: BHF-UCL
  • regulation of sphingolipid mediated signaling pathway Source: MGI
  • regulation of Wnt signaling pathway Source: MGI
  • single organismal cell-cell adhesion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase

Enzyme and pathway databases

ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid phosphatase 3By similarity (EC:3.1.3.4)
Alternative name(s):
Lipid phosphate phosphohydrolase 3
PAP2-beta
Phosphatidate phosphohydrolase type 2b
Phosphatidic acid phosphatase 2b
Short name:
PAP-2b
Short name:
PAP2b
Gene namesi
Name:Plpp3By similarity
Synonyms:Lpp3, Ppap2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1915166. Plpp3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 85LumenalSequence analysisAdd BLAST31
Transmembranei86 – 106HelicalSequence analysisAdd BLAST21
Topological domaini107 – 123CytoplasmicSequence analysisAdd BLAST17
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Topological domaini145 – 194LumenalSequence analysisAdd BLAST50
Transmembranei195 – 215HelicalSequence analysisAdd BLAST21
Topological domaini216 – 226CytoplasmicSequence analysisAdd BLAST11
Transmembranei227 – 247HelicalSequence analysisAdd BLAST21
Topological domaini248 – 258LumenalSequence analysisAdd BLAST11
Transmembranei259 – 279HelicalSequence analysisAdd BLAST21
Topological domaini280 – 312CytoplasmicSequence analysisAdd BLAST33

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Involvement in diseasei

Ppap2b deficient embryos fail to form a chorioallantoic placenta and yolk sac vasculature. A subset of embryos also show a shortening of the anterior-posterior axis and frequent duplication of axial structures. Loss of Ppap2b results in a marked increase in beta-catenin-mediated T-cell factor (TCF) transcription.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002209131 – 312Phospholipid phosphatase 3Add BLAST312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19PhosphoserineBy similarity1
Glycosylationi171N-linked (GlcNAc...)Sequence analysis1
Modified residuei298PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ99JY8.
PaxDbiQ99JY8.
PeptideAtlasiQ99JY8.
PRIDEiQ99JY8.

PTM databases

iPTMnetiQ99JY8.
PhosphoSitePlusiQ99JY8.
SwissPalmiQ99JY8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028517.
CleanExiMM_PPAP2B.
GenevisibleiQ99JY8. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

  • integrin binding Source: MGI

Protein-protein interaction databases

IntActiQ99JY8. 5 interactors.
MINTiMINT-4612030.
STRINGi10090.ENSMUSP00000065719.

Structurei

3D structure databases

ProteinModelPortaliQ99JY8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410ITB4. Eukaryota.
ENOG4111NKF. LUCA.
GeneTreeiENSGT00620000087654.
HOGENOMiHOG000041307.
HOVERGENiHBG002048.
InParanoidiQ99JY8.
KOiK01080.
OMAiNNNCKDH.
OrthoDBiEOG091G0K5H.
PhylomeDBiQ99JY8.
TreeFamiTF316040.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR028675. LPP3.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PANTHERiPTHR10165:SF79. PTHR10165:SF79. 1 hit.
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q99JY8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSYKYDKAI VPESKNGGSP ALNNNPRKGG SKRVLLICLD LFCLFMAALP
60 70 80 90 100
FLIIETSTIK PYRRGFYCND ESIKYPLKVS ETINDAVLCA VGIVIAILAI
110 120 130 140 150
ITGEFYRIYY LKEKSRSTTQ NPYVAALYKQ VGCFLFGCAI SQSFTDIAKV
160 170 180 190 200
SIGRLRPHFL SVCDPDFSQI NCSEGYIQNY RCRGEDSKVQ EARKSFFSGH
210 220 230 240 250
ASFSMFTMLY LVLYLQARFT WRGARLLRPL LQFTLLMMAF YTGLSRVSDY
260 270 280 290 300
KHHPSDVLAG FAQGALVACC IVFFVSDLFK TKTSLSLPAP AIRREILSPV
310
DIIDRNNHHN MV
Length:312
Mass (Da):35,216
Last modified:June 1, 2001 - v1
Checksum:iD782986E04B57D7D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187S → C in BAE34848 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159136 mRNA. Translation: BAE34848.1.
AK160056 mRNA. Translation: BAE35594.1.
BC005558 mRNA. Translation: AAH05558.1.
CCDSiCCDS18417.1.
RefSeqiNP_542122.1. NM_080555.2.
UniGeneiMm.348326.

Genome annotation databases

EnsembliENSMUST00000064139; ENSMUSP00000065719; ENSMUSG00000028517.
GeneIDi67916.
KEGGimmu:67916.
UCSCiuc008tye.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159136 mRNA. Translation: BAE34848.1.
AK160056 mRNA. Translation: BAE35594.1.
BC005558 mRNA. Translation: AAH05558.1.
CCDSiCCDS18417.1.
RefSeqiNP_542122.1. NM_080555.2.
UniGeneiMm.348326.

3D structure databases

ProteinModelPortaliQ99JY8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99JY8. 5 interactors.
MINTiMINT-4612030.
STRINGi10090.ENSMUSP00000065719.

PTM databases

iPTMnetiQ99JY8.
PhosphoSitePlusiQ99JY8.
SwissPalmiQ99JY8.

Proteomic databases

MaxQBiQ99JY8.
PaxDbiQ99JY8.
PeptideAtlasiQ99JY8.
PRIDEiQ99JY8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064139; ENSMUSP00000065719; ENSMUSG00000028517.
GeneIDi67916.
KEGGimmu:67916.
UCSCiuc008tye.1. mouse.

Organism-specific databases

CTDi8613.
MGIiMGI:1915166. Plpp3.

Phylogenomic databases

eggNOGiENOG410ITB4. Eukaryota.
ENOG4111NKF. LUCA.
GeneTreeiENSGT00620000087654.
HOGENOMiHOG000041307.
HOVERGENiHBG002048.
InParanoidiQ99JY8.
KOiK01080.
OMAiNNNCKDH.
OrthoDBiEOG091G0K5H.
PhylomeDBiQ99JY8.
TreeFamiTF316040.

Enzyme and pathway databases

ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

ChiTaRSiPpap2b. mouse.
PROiQ99JY8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028517.
CleanExiMM_PPAP2B.
GenevisibleiQ99JY8. MM.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR028675. LPP3.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PANTHERiPTHR10165:SF79. PTHR10165:SF79. 1 hit.
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLPP3_MOUSE
AccessioniPrimary (citable) accession number: Q99JY8
Secondary accession number(s): Q3TVM4, Q3TXR7, Q8BTB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.