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Protein

Trifunctional enzyme subunit beta, mitochondrial

Gene

Hadhb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei139Acyl-thioester intermediateBy similarity1
Active sitei429Proton acceptorPROSITE-ProRule annotation1
Active sitei459Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1482798 Acyl chain remodeling of CL
R-MMU-77285 Beta oxidation of myristoyl-CoA to lauroyl-CoA
R-MMU-77305 Beta oxidation of palmitoyl-CoA to myristoyl-CoA
R-MMU-77310 Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA
R-MMU-77346 Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA
R-MMU-77348 Beta oxidation of octanoyl-CoA to hexanoyl-CoA
R-MMU-77350 Beta oxidation of hexanoyl-CoA to butanoyl-CoA
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional enzyme subunit beta, mitochondrial
Alternative name(s):
TP-beta
Including the following 1 domains:
3-ketoacyl-CoA thiolase (EC:2.3.1.16)
Alternative name(s):
Acetyl-CoA acyltransferase
Beta-ketothiolase
Gene namesi
Name:Hadhb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2136381 Hadhb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 34MitochondrionBy similarityAdd BLAST34
ChainiPRO_000003408235 – 475Trifunctional enzyme subunit beta, mitochondrialAdd BLAST441

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N6-succinyllysineCombined sources1
Modified residuei73N6-acetyllysine; alternateCombined sources1
Modified residuei73N6-succinyllysine; alternateCombined sources1
Modified residuei189N6-acetyllysine; alternateCombined sources1
Modified residuei189N6-succinyllysine; alternateCombined sources1
Modified residuei191N6-succinyllysineCombined sources1
Modified residuei273N6-succinyllysineCombined sources1
Modified residuei292N6-succinyllysineCombined sources1
Modified residuei294N6-acetyllysine; alternateCombined sources1
Modified residuei294N6-succinyllysine; alternateCombined sources1
Modified residuei299N6-acetyllysineCombined sources1
Modified residuei333N6-acetyllysine; alternateCombined sources1
Modified residuei333N6-succinyllysine; alternateCombined sources1
Modified residuei349N6-acetyllysineCombined sources1
Modified residuei362N6-acetyllysineCombined sources1

Post-translational modificationi

Acetylation of Lys-202 is observed in liver mitochondria from fasted mice but not from fed mice.

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ99JY0
MaxQBiQ99JY0
PaxDbiQ99JY0
PeptideAtlasiQ99JY0
PRIDEiQ99JY0

PTM databases

iPTMnetiQ99JY0
PhosphoSitePlusiQ99JY0
SwissPalmiQ99JY0

Expressioni

Gene expression databases

BgeeiENSMUSG00000059447
ExpressionAtlasiQ99JY0 baseline and differential
GenevisibleiQ99JY0 MM

Interactioni

Subunit structurei

Octamer of 4 alpha (HADHA) and 4 beta (HADHB) subunits. Interacts with RSAD2/viperin.By similarity

Protein-protein interaction databases

BioGridi231080, 3 interactors
IntActiQ99JY0, 6 interactors
MINTiQ99JY0
STRINGi10090.ENSMUSP00000026841

Structurei

3D structure databases

ProteinModelPortaliQ99JY0
SMRiQ99JY0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1392 Eukaryota
COG0183 LUCA
GeneTreeiENSGT00910000144143
HOGENOMiHOG000012240
HOVERGENiHBG104782
InParanoidiQ99JY0
KOiK07509
OMAiMTAFPEP
OrthoDBiEOG091G09R7
PhylomeDBiQ99JY0
TreeFamiTF315243

Family and domain databases

CDDicd00751 thiolase, 1 hit
Gene3Di3.40.47.10, 1 hit
InterProiView protein in InterPro
IPR002155 Thiolase
IPR016039 Thiolase-like
IPR020615 Thiolase_acyl_enz_int_AS
IPR020610 Thiolase_AS
IPR020617 Thiolase_C
IPR020613 Thiolase_CS
IPR020616 Thiolase_N
PfamiView protein in Pfam
PF02803 Thiolase_C, 1 hit
PF00108 Thiolase_N, 1 hit
SUPFAMiSSF53901 SSF53901, 2 hits
TIGRFAMsiTIGR01930 AcCoA-C-Actrans, 1 hit
PROSITEiView protein in PROSITE
PS00098 THIOLASE_1, 1 hit
PS00737 THIOLASE_2, 1 hit
PS00099 THIOLASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99JY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTILTSTFR NLSTTSKWAL RSSIRPLSCS SQLHSAPAVQ TKSKKTLAKP
60 70 80 90 100
NMKNIVVVEG VRIPFLLSGT SYKDLMPHDL ARAALSGLLH RTNIPKDVVD
110 120 130 140 150
YIIFGTVIQE VKTSNVAREA ALGAGFSDKT PAHTVTMACI SSNQAMTTAV
160 170 180 190 200
GLIASGQCDV VVAGGVELMS DVPIRHSRNM RKMMLDLNKA KTLGQRLSLL
210 220 230 240 250
SKFRLNFLSP ELPAVAEFST NETMGHSADR LAAAFAVSRM EQDEYALRSH
260 270 280 290 300
SLAKKAQDEG HLSDIVPFKV PGKDTVTKDN GIRPSSLEQM AKLKPAFIKP
310 320 330 340 350
YGTVTAANSS FLTDGASAML IMSEDRALAM GYKPKAYLRD FIYVSQDPKD
360 370 380 390 400
QLLLGPTYAT PKVLEKAGLT MNDIDAFEFH EAFSGQILAN FKAMDSDWFA
410 420 430 440 450
QNYMGRKTKV GSPPLEKFNI WGGSLSLGHP FGATGCRLVM AAANRLRKDG
460 470
GQYALVAACA AGGQGHAMIV EAYPK
Length:475
Mass (Da):51,386
Last modified:June 1, 2001 - v1
Checksum:iF131B497C4F5FAF4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24 – 25IR → HK in BAC36493 (PubMed:16141072).Curated2
Sequence conflicti425L → M in BAC38790 (PubMed:16141072).Curated1
Sequence conflicti450G → R in BAC38790 (PubMed:16141072).Curated1
Sequence conflicti450G → V in BAC39015 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033462 mRNA Translation: BAC28300.1
AK076814 mRNA Translation: BAC36493.1
AK083164 mRNA Translation: BAC38790.1
AK083767 mRNA Translation: BAC39015.1
AK150889 mRNA Translation: BAE29936.1
AK169637 mRNA Translation: BAE41269.1
BC005585 mRNA Translation: AAH05585.1
CCDSiCCDS39045.1
RefSeqiNP_001276727.1, NM_001289798.1
NP_001276728.1, NM_001289799.1
NP_663533.1, NM_145558.2
XP_017176317.1, XM_017320828.1
XP_017176318.1, XM_017320829.1
UniGeneiMm.291463
Mm.389348

Genome annotation databases

EnsembliENSMUST00000026841; ENSMUSP00000026841; ENSMUSG00000059447
ENSMUST00000114783; ENSMUSP00000110431; ENSMUSG00000059447
ENSMUST00000114786; ENSMUSP00000110434; ENSMUSG00000059447
GeneIDi231086
KEGGimmu:231086
UCSCiuc008wve.2 mouse

Similar proteinsi

Entry informationi

Entry nameiECHB_MOUSE
AccessioniPrimary (citable) accession number: Q99JY0
Secondary accession number(s): Q3TEH9
, Q8BJI5, Q8BJM0, Q8BK52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 2001
Last modified: June 20, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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