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Protein

Golgi reassembly-stacking protein 2

Gene

Gorasp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.1 Publication

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-162658. Golgi Cisternae Pericentriolar Stack Reorganization.

Protein family/group databases

TCDBi9.A.48.1.1. the unconventional protein secretion (ups) system.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi reassembly-stacking protein 2
Short name:
GRS2
Alternative name(s):
Golgi reassembly-stacking protein of 55 kDa
Short name:
GRASP55
Gene namesi
Name:Gorasp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2135962. Gorasp2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000875462 – 451Golgi reassembly-stacking protein 2Add BLAST450

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei30Dimethylated arginine; alternateBy similarity1
Modified residuei30Omega-N-methylated arginine; alternateBy similarity1
Modified residuei47Dimethylated arginine; alternateBy similarity1
Modified residuei47Omega-N-methylated arginine; alternateBy similarity1
Modified residuei214PhosphoserineBy similarity1
Modified residuei222PhosphothreonineBy similarity1
Modified residuei225Phosphothreonine; by MAPKBy similarity1
Modified residuei411PhosphoserineCombined sources1
Modified residuei417PhosphothreonineBy similarity1
Modified residuei435PhosphothreonineBy similarity1
Modified residuei443PhosphoserineCombined sources1
Modified residuei448PhosphoserineBy similarity1

Post-translational modificationi

Myristoylated. Myristoylation is essential for the Golgi targeting (By similarity).By similarity
Palmitoylated.By similarity
Phosphorylated in mitotic cells.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Myristate, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ99JX3.
PaxDbiQ99JX3.
PeptideAtlasiQ99JX3.
PRIDEiQ99JX3.

PTM databases

iPTMnetiQ99JX3.
PhosphoSitePlusiQ99JX3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000014959.
CleanExiMM_GORASP2.
ExpressionAtlasiQ99JX3. baseline and differential.
GenevisibleiQ99JX3. MM.

Interactioni

Subunit structurei

Forms a RAB2 effector complex with BLZF1/Golgin 45 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of transmembrane TGFA, prior its transit in the trans-Golgi. Does not interact with GM130. Interacts with KCTD5. Interacts with TMED2 and TMED3 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ99JX3. 2 interactors.
MINTiMINT-4096444.
STRINGi10090.ENSMUSP00000028509.

Structurei

Secondary structure

1451
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 4Combined sources3
Beta strandi14 – 22Combined sources9
Helixi27 – 30Combined sources4
Turni35 – 37Combined sources3
Beta strandi38 – 43Combined sources6
Beta strandi50 – 53Combined sources4
Helixi54 – 61Combined sources8
Turni62 – 64Combined sources3
Beta strandi67 – 73Combined sources7
Turni74 – 76Combined sources3
Beta strandi79 – 84Combined sources6
Beta strandi90 – 96Combined sources7
Beta strandi98 – 104Combined sources7
Helixi108 – 110Combined sources3
Beta strandi113 – 118Combined sources6
Helixi123 – 127Combined sources5
Turni131 – 133Combined sources3
Beta strandi134 – 141Combined sources8
Beta strandi143 – 145Combined sources3
Helixi148 – 155Combined sources8
Turni156 – 158Combined sources3
Beta strandi161 – 167Combined sources7
Turni168 – 171Combined sources4
Beta strandi172 – 178Combined sources7
Beta strandi184 – 190Combined sources7
Beta strandi192 – 195Combined sources4
Helixi198 – 200Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GMIX-ray2.71A/B2-208[»]
5GMJX-ray2.99A/B2-208[»]
5GMLX-ray2.55A/B2-208[»]
5H3JX-ray1.33A2-208[»]
ProteinModelPortaliQ99JX3.
SMRiQ99JX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 75PDZAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 199Important for membrane bindingBy similarity6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi278 – 377Pro-richAdd BLAST100

Sequence similaritiesi

Belongs to the GORASP family.Curated

Phylogenomic databases

eggNOGiKOG3834. Eukaryota.
COG5233. LUCA.
GeneTreeiENSGT00390000008686.
HOGENOMiHOG000231920.
HOVERGENiHBG051826.
InParanoidiQ99JX3.
OMAiMPSEFLP.
OrthoDBiEOG091G0E90.
PhylomeDBiQ99JX3.
TreeFamiTF314053.

Family and domain databases

InterProiView protein in InterPro
IPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR036034. PDZ_sf.
PANTHERiPTHR12893. PTHR12893. 1 hit.
PfamiView protein in Pfam
PF04495. GRASP55_65. 1 hit.
SUPFAMiSSF50156. SSF50156. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99JX3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSSQSVEIP GGGTEGYHVL RVQENSPGHR AGLEPFFDFI VSINGSRLNK
60 70 80 90 100
DNDTLKDLLK ANVEKPVKML IYSSKTLELR EASVTPSNLW GGQGLLGVSI
110 120 130 140 150
RFCSFDGANE NVWHVLEVES NSPAALAGLR PHSDYIIGAD TVMNESEDLF
160 170 180 190 200
SLIETHEAKP LKLYVYNTDT DNCREVIITP NSAWGGEGSL GCGIGYGYLH
210 220 230 240 250
RIPTRPFEEG KKISLPGQMT GTPITPLKDG FTEVQLSSVS PPSLSPPGTT
260 270 280 290 300
GVEQSLSGLS ISSAPPAVSN VLSTGVPTVP LLPPQVNQSL ASMPPMNPAT
310 320 330 340 350
TLPSLMPLSA GLPSLPNLPS LSNFNLPAPH IMPGVGLPEL GSPGLPPLPS
360 370 380 390 400
LPPRNLPGIA PLPMLSDFLP SFPLVPEGSS AASAGEPLSS LPAMGPPSDP
410 420 430 440 450
VMTTAKADAS SLTVDVTSPA SKVPTTVEDR VSDCTPAVEK PVSDADASEP

S
Length:451
Mass (Da):47,038
Last modified:January 23, 2007 - v3
Checksum:i7E37234E827B459A
GO
Isoform 2 (identifier: Q99JX3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Show »
Length:383
Mass (Da):39,661
Checksum:i0E80108C0BEA14E8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93Q → L in AAH16455 (PubMed:15489334).Curated1
Sequence conflicti352P → L in BAC28276 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0113011 – 68Missing in isoform 2. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033413 mRNA. Translation: BAC28276.1.
AK077683 mRNA. Translation: BAC36954.1.
AK077706 mRNA. Translation: BAC36969.1.
AK148337 mRNA. Translation: BAE28492.1.
AK151843 mRNA. Translation: BAE30735.1.
AK152475 mRNA. Translation: BAE31249.1.
AK152544 mRNA. Translation: BAE31299.1.
AK152603 mRNA. Translation: BAE31351.1.
AK153049 mRNA. Translation: BAE31677.1.
AK169382 mRNA. Translation: BAE41129.1.
BC005600 mRNA. Translation: AAH05600.1.
BC016455 mRNA. Translation: AAH16455.1.
CCDSiCCDS16109.1. [Q99JX3-1]
RefSeqiNP_081628.3. NM_027352.4. [Q99JX3-1]
UniGeneiMm.271950.
Mm.470827.

Genome annotation databases

EnsembliENSMUST00000028509; ENSMUSP00000028509; ENSMUSG00000014959. [Q99JX3-1]
GeneIDi70231.
KEGGimmu:70231.
UCSCiuc008jzo.2. mouse. [Q99JX3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGORS2_MOUSE
AccessioniPrimary (citable) accession number: Q99JX3
Secondary accession number(s): Q3U9D2, Q8CCD0, Q91W68
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families