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Q99JW5 (EPCAM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Epithelial cell adhesion molecule

Short name=Ep-CAM
Alternative name(s):
Epithelial glycoprotein 314
Short name=EGP314
Short name=mEGP314
Protein 289A
Tumor-associated calcium signal transducer 1
CD_antigen=CD326
Gene names
Name:Epcam
Synonyms:Tacstd1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E By similarity.

Subunit structure

Monomer. Interacts with phosphorylated CLDN7 By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein By similarity. Lateral cell membrane By similarity. Cell junctiontight junction By similarity. Note: Colocalizes with CLDN7 at the lateral cell membrane and tight junction By similarity.

Post-translational modification

Glycosylation at Asn-198 is crucial for protein stability By similarity.

Sequence similarities

Belongs to the EPCAM family.

Contains 1 thyroglobulin type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 315292Epithelial cell adhesion molecule
PRO_0000380183

Regions

Topological domain24 – 266243Extracellular Potential
Transmembrane267 – 28923Helical; Potential
Topological domain290 – 31526Cytoplasmic Potential
Domain63 – 13573Thyroglobulin type-1

Amino acid modifications

Glycosylation1111N-linked (GlcNAc...) Potential
Glycosylation1981N-linked (GlcNAc...) Potential
Disulfide bond27 ↔ 46 By similarity
Disulfide bond29 ↔ 59 By similarity
Disulfide bond38 ↔ 48 By similarity
Disulfide bond66 ↔ 99 By similarity
Disulfide bond110 ↔ 116 By similarity
Disulfide bond118 ↔ 135 By similarity

Experimental info

Sequence conflict871Missing in AAA37543. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q99JW5 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: FDA853E165BA8906

FASTA31535,019
        10         20         30         40         50         60 
MAGPQALAFG LLLAVVTATL AAAQRDCVCD NYKLATSCSL NEYGECQCTS YGTQNTVICS 

        70         80         90        100        110        120 
KLASKCLAMK AEMTHSKSGR RIKPEGAIQN NDGLYDPDCD EQGLFKAKQC NGTATCWCVN 

       130        140        150        160        170        180 
TAGVRRTDKD TEITCSERVR TYWIIIELKH KERESPYDHQ SLQTALQEAF TSRYKLNQKF 

       190        200        210        220        230        240 
IKNIMYENNV ITIDLMQNSS QKTQDDVDIA DVAYYFEKDV KGESLFHSSK SMDLRVNGEP 

       250        260        270        280        290        300 
LDLDPGQTLI YYVDEKAPEF SMQGLTAGII AVIVVVSLAV IAGIVVLVIS TRKKSAKYEK 

       310 
AEIKEMGEIH RELNA 

« Hide

References

« Hide 'large scale' references
[1]"A murine cDNA encodes a pan-epithelial glycoprotein that is also expressed on plasma cells."
Bergsagel P.L., Victor-Kobrin C., Timblin C.R., Trepel J., Kuehl W.M.
J. Immunol. 148:590-596(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M76124 mRNA. Translation: AAA37543.1.
AK145752 mRNA. Translation: BAE26627.1.
AK167182 mRNA. Translation: BAE39317.1.
BC005618 mRNA. Translation: AAH05618.1.
BC094465 mRNA. Translation: AAH94465.1.
RefSeqNP_032558.2. NM_008532.2.
UniGeneMm.4259.

3D structure databases

ProteinModelPortalQ99JW5.
SMRQ99JW5. Positions 93-137.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ99JW5. 3 interactions.

Proteomic databases

PaxDbQ99JW5.
PRIDEQ99JW5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000053577; ENSMUSP00000061935; ENSMUSG00000045394.
GeneID17075.
KEGGmmu:17075.
UCSCuc008duy.1. mouse.

Organism-specific databases

CTD4072.
MGIMGI:106653. Epcam.

Phylogenomic databases

eggNOGNOG46689.
GeneTreeENSGT00390000018245.
HOGENOMHOG000074086.
InParanoidQ61512.
KOK06737.
OMAMQGLQAG.
OrthoDBEOG7N0C5D.
PhylomeDBQ99JW5.
TreeFamTF332767.

Gene expression databases

ArrayExpressQ99JW5.
BgeeQ99JW5.
GenevestigatorQ99JW5.

Family and domain databases

Gene3D4.10.800.10. 1 hit.
InterProIPR000716. Thyroglobulin_1.
[Graphical view]
PfamPF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
SMARTSM00211. TY. 1 hit.
[Graphical view]
SUPFAMSSF57610. SSF57610. 1 hit.
PROSITEPS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291194.
PROQ99JW5.
SOURCESearch...

Entry information

Entry nameEPCAM_MOUSE
AccessionPrimary (citable) accession number: Q99JW5
Secondary accession number(s): Q61512
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot