SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q99JW5

- EPCAM_MOUSE

UniProt

Q99JW5 - EPCAM_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Epithelial cell adhesion molecule

Gene
Epcam, Tacstd1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E By similarity.

GO - Biological processi

  1. positive regulation of cell proliferation Source: UniProtKB
  2. ureteric bud development Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Epithelial cell adhesion molecule
Short name:
Ep-CAM
Alternative name(s):
Epithelial glycoprotein 314
Short name:
EGP314
Short name:
mEGP314
Protein 289A
Tumor-associated calcium signal transducer 1
CD_antigen: CD326
Gene namesi
Name:Epcam
Synonyms:Tacstd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:106653. Epcam.

Subcellular locationi

Membrane; Single-pass type I membrane protein By similarity. Lateral cell membrane By similarity. Cell junctiontight junction By similarity
Note: Colocalizes with CLDN7 at the lateral cell membrane and tight junction By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 266243Extracellular Reviewed predictionAdd
BLAST
Transmembranei267 – 28923Helical; Reviewed predictionAdd
BLAST
Topological domaini290 – 31526Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: MGI
  2. basolateral plasma membrane Source: MGI
  3. cell surface Source: MGI
  4. integral component of membrane Source: UniProtKB-KW
  5. lateral plasma membrane Source: UniProtKB
  6. plasma membrane Source: UniProtKB
  7. tight junction Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed predictionAdd
BLAST
Chaini24 – 315292Epithelial cell adhesion moleculePRO_0000380183Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi27 ↔ 46 By similarity
Disulfide bondi29 ↔ 59 By similarity
Disulfide bondi38 ↔ 48 By similarity
Disulfide bondi66 ↔ 99 By similarity
Disulfide bondi110 ↔ 116 By similarity
Glycosylationi111 – 1111N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi118 ↔ 135 By similarity
Glycosylationi198 – 1981N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Glycosylation at Asn-198 is crucial for protein stability By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ99JW5.
PaxDbiQ99JW5.
PRIDEiQ99JW5.

Expressioni

Gene expression databases

ArrayExpressiQ99JW5.
BgeeiQ99JW5.
GenevestigatoriQ99JW5.

Interactioni

Subunit structurei

Monomer. Interacts with phosphorylated CLDN7 By similarity.

Protein-protein interaction databases

IntActiQ99JW5. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ99JW5.
SMRiQ99JW5. Positions 93-137.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 13573Thyroglobulin type-1Add
BLAST

Sequence similaritiesi

Belongs to the EPCAM family.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46689.
GeneTreeiENSGT00390000018245.
HOGENOMiHOG000074086.
InParanoidiQ61512.
KOiK06737.
OMAiMQGLQAG.
OrthoDBiEOG7N0C5D.
PhylomeDBiQ99JW5.
TreeFamiTF332767.

Family and domain databases

Gene3Di4.10.800.10. 1 hit.
InterProiIPR000716. Thyroglobulin_1.
[Graphical view]
PfamiPF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
SMARTiSM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57610. SSF57610. 1 hit.
PROSITEiPS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99JW5-1 [UniParc]FASTAAdd to Basket

« Hide

MAGPQALAFG LLLAVVTATL AAAQRDCVCD NYKLATSCSL NEYGECQCTS    50
YGTQNTVICS KLASKCLAMK AEMTHSKSGR RIKPEGAIQN NDGLYDPDCD 100
EQGLFKAKQC NGTATCWCVN TAGVRRTDKD TEITCSERVR TYWIIIELKH 150
KERESPYDHQ SLQTALQEAF TSRYKLNQKF IKNIMYENNV ITIDLMQNSS 200
QKTQDDVDIA DVAYYFEKDV KGESLFHSSK SMDLRVNGEP LDLDPGQTLI 250
YYVDEKAPEF SMQGLTAGII AVIVVVSLAV IAGIVVLVIS TRKKSAKYEK 300
AEIKEMGEIH RELNA 315
Length:315
Mass (Da):35,019
Last modified:June 1, 2001 - v1
Checksum:iFDA853E165BA8906
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871Missing in AAA37543. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76124 mRNA. Translation: AAA37543.1.
AK145752 mRNA. Translation: BAE26627.1.
AK167182 mRNA. Translation: BAE39317.1.
BC005618 mRNA. Translation: AAH05618.1.
BC094465 mRNA. Translation: AAH94465.1.
CCDSiCCDS29018.1.
RefSeqiNP_032558.2. NM_008532.2.
UniGeneiMm.4259.

Genome annotation databases

EnsembliENSMUST00000053577; ENSMUSP00000061935; ENSMUSG00000045394.
GeneIDi17075.
KEGGimmu:17075.
UCSCiuc008duy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76124 mRNA. Translation: AAA37543.1 .
AK145752 mRNA. Translation: BAE26627.1 .
AK167182 mRNA. Translation: BAE39317.1 .
BC005618 mRNA. Translation: AAH05618.1 .
BC094465 mRNA. Translation: AAH94465.1 .
CCDSi CCDS29018.1.
RefSeqi NP_032558.2. NM_008532.2.
UniGenei Mm.4259.

3D structure databases

ProteinModelPortali Q99JW5.
SMRi Q99JW5. Positions 93-137.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q99JW5. 3 interactions.

Proteomic databases

MaxQBi Q99JW5.
PaxDbi Q99JW5.
PRIDEi Q99JW5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000053577 ; ENSMUSP00000061935 ; ENSMUSG00000045394 .
GeneIDi 17075.
KEGGi mmu:17075.
UCSCi uc008duy.1. mouse.

Organism-specific databases

CTDi 4072.
MGIi MGI:106653. Epcam.

Phylogenomic databases

eggNOGi NOG46689.
GeneTreei ENSGT00390000018245.
HOGENOMi HOG000074086.
InParanoidi Q61512.
KOi K06737.
OMAi MQGLQAG.
OrthoDBi EOG7N0C5D.
PhylomeDBi Q99JW5.
TreeFami TF332767.

Miscellaneous databases

NextBioi 291194.
PROi Q99JW5.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q99JW5.
Bgeei Q99JW5.
Genevestigatori Q99JW5.

Family and domain databases

Gene3Di 4.10.800.10. 1 hit.
InterProi IPR000716. Thyroglobulin_1.
[Graphical view ]
Pfami PF00086. Thyroglobulin_1. 1 hit.
[Graphical view ]
SMARTi SM00211. TY. 1 hit.
[Graphical view ]
SUPFAMi SSF57610. SSF57610. 1 hit.
PROSITEi PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A murine cDNA encodes a pan-epithelial glycoprotein that is also expressed on plasma cells."
    Bergsagel P.L., Victor-Kobrin C., Timblin C.R., Trepel J., Kuehl W.M.
    J. Immunol. 148:590-596(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiEPCAM_MOUSE
AccessioniPrimary (citable) accession number: Q99JW5
Secondary accession number(s): Q61512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi