Q99JW5 (EPCAM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Epithelial cell adhesion molecule
Short name=Ep-CAM

Alternative name(s):
Epithelial glycoprotein 314
Short name=EGP314
Short name=mEGP314
Protein 289A
Tumor-associated calcium signal transducer 1
CD_antigen=CD326

Gene names

Name:	Epcam
Synonyms:	Tacstd1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E By similarity.
Subunit structure	Monomer. Interacts with phosphorylated CLDN7 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein By similarity. Lateral cell membrane By similarity. Cell junction › tight junction By similarity. Note: Co-localizes with CLDN7 at the lateral cell membrane and tight junction By similarity.
Post-translational modification	Glycosylation at Asn-198 is crucial for protein stability By similarity.
Sequence similarities	Belongs to the EPCAM family. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Tight junction
Domain	Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB ureteric bud development Inferred from expression pattern PubMed 16545622. Source: UniProtKB
Cellular_component	apical plasma membrane Inferred from sequence or structural similarity PubMed 18025791. Source: MGI basolateral plasma membrane Inferred from sequence or structural similarity PubMed 18025791. Source: MGI cell surface Inferred from direct assay PubMed 17599412. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lateral plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB tight junction Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 315	292	Epithelial cell adhesion molecule		PRO_0000380183
Regions
	Topological domain	24 – 266	243	Extracellular Potential
	Transmembrane	267 – 289	23	Helical; Potential
	Topological domain	290 – 315	26	Cytoplasmic Potential
	Domain	63 – 135	73	Thyroglobulin type-1
Amino acid modifications
	Glycosylation	111	1	N-linked (GlcNAc...) Potential
	Glycosylation	198	1	N-linked (GlcNAc...) Potential
	Disulfide bond	27 ↔ 46		By similarity
	Disulfide bond	29 ↔ 59		By similarity
	Disulfide bond	38 ↔ 48		By similarity
	Disulfide bond	66 ↔ 99		By similarity
	Disulfide bond	110 ↔ 116		By similarity
	Disulfide bond	118 ↔ 135		By similarity
Experimental info
	Sequence conflict	87	1	Missing in AAA37543. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99JW5 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: FDA853E165BA8906	FASTA	315	35,019
	10 20 30 40 50 60 MAGPQALAFG LLLAVVTATL AAAQRDCVCD NYKLATSCSL NEYGECQCTS YGTQNTVICS 70 80 90 100 110 120 KLASKCLAMK AEMTHSKSGR RIKPEGAIQN NDGLYDPDCD EQGLFKAKQC NGTATCWCVN 130 140 150 160 170 180 TAGVRRTDKD TEITCSERVR TYWIIIELKH KERESPYDHQ SLQTALQEAF TSRYKLNQKF 190 200 210 220 230 240 IKNIMYENNV ITIDLMQNSS QKTQDDVDIA DVAYYFEKDV KGESLFHSSK SMDLRVNGEP 250 260 270 280 290 300 LDLDPGQTLI YYVDEKAPEF SMQGLTAGII AVIVVVSLAV IAGIVVLVIS TRKKSAKYEK 310 AEIKEMGEIH RELNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A murine cDNA encodes a pan-epithelial glycoprotein that is also expressed on plasma cells." Bergsagel P.L., Victor-Kobrin C., Timblin C.R., Trepel J., Kuehl W.M. J. Immunol. 148:590-596(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M76124 mRNA. Translation: AAA37543.1. AK145752 mRNA. Translation: BAE26627.1. AK167182 mRNA. Translation: BAE39317.1. BC005618 mRNA. Translation: AAH05618.1. BC094465 mRNA. Translation: AAH94465.1.
IPI	IPI00115558.
RefSeq	NP_032558.2. NM_008532.2.
UniGene	Mm.4259.
3D structure databases
ProteinModelPortal	Q99JW5.
SMR	Q99JW5. Positions 92-137.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99JW5. 3 interactions.
Proteomic databases
PaxDb	Q99JW5.
PRIDE	Q99JW5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000053577; ENSMUSP00000061935; ENSMUSG00000045394.
GeneID	17075.
KEGG	mmu:17075.
UCSC	uc008duy.1. mouse.
Organism-specific databases
CTD	4072.
MGI	MGI:106653. Epcam.
Phylogenomic databases
eggNOG	NOG46689.
GeneTree	ENSGT00390000018245.
HOGENOM	HOG000074086.
InParanoid	Q61512.
KO	K06737.
OMA	PPEFSMQ.
OrthoDB	EOG4V6ZHK.
Gene expression databases
ArrayExpress	Q99JW5.
Bgee	Q99JW5.
Genevestigator	Q99JW5.
Family and domain databases
Gene3D	4.10.800.10. 1 hit.
InterPro	IPR000716. Thyroglobulin_1. [Graphical view]
Pfam	PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
SMART	SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291194.
SOURCE	Search...

Entry information

Entry name

EPCAM_MOUSE

Accession

Primary (citable) accession number: Q99JW5
Secondary accession number(s): Q61512

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program