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Q99JW5

- EPCAM_MOUSE

UniProt

Q99JW5 - EPCAM_MOUSE

Protein

Epithelial cell adhesion molecule

Gene

Epcam

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E By similarity.By similarity

    GO - Biological processi

    1. positive regulation of cell proliferation Source: UniProtKB
    2. ureteric bud development Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epithelial cell adhesion molecule
    Short name:
    Ep-CAM
    Alternative name(s):
    Epithelial glycoprotein 314
    Short name:
    EGP314
    Short name:
    mEGP314
    Protein 289A
    Tumor-associated calcium signal transducer 1
    CD_antigen: CD326
    Gene namesi
    Name:Epcam
    Synonyms:Tacstd1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:106653. Epcam.

    Subcellular locationi

    Membrane By similarity; Single-pass type I membrane protein By similarity. Lateral cell membrane By similarity. Cell junctiontight junction By similarity
    Note: Colocalizes with CLDN7 at the lateral cell membrane and tight junction.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: MGI
    2. basolateral plasma membrane Source: MGI
    3. cell surface Source: MGI
    4. integral component of membrane Source: UniProtKB-KW
    5. lateral plasma membrane Source: UniProtKB
    6. plasma membrane Source: UniProtKB
    7. tight junction Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Tight junction

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 315292Epithelial cell adhesion moleculePRO_0000380183Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi27 ↔ 46PROSITE-ProRule annotation
    Disulfide bondi29 ↔ 59PROSITE-ProRule annotation
    Disulfide bondi38 ↔ 48PROSITE-ProRule annotation
    Disulfide bondi66 ↔ 99PROSITE-ProRule annotation
    Disulfide bondi110 ↔ 116PROSITE-ProRule annotation
    Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi118 ↔ 135PROSITE-ProRule annotation
    Glycosylationi198 – 1981N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylation at Asn-198 is crucial for protein stability.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ99JW5.
    PaxDbiQ99JW5.
    PRIDEiQ99JW5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99JW5.
    BgeeiQ99JW5.
    GenevestigatoriQ99JW5.

    Interactioni

    Subunit structurei

    Monomer. Interacts with phosphorylated CLDN7 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ99JW5. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99JW5.
    SMRiQ99JW5. Positions 93-137.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 266243ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini290 – 31526CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei267 – 28923HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini63 – 13573Thyroglobulin type-1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the EPCAM family.Curated
    Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46689.
    GeneTreeiENSGT00390000018245.
    HOGENOMiHOG000074086.
    InParanoidiQ61512.
    KOiK06737.
    OMAiMQGLQAG.
    OrthoDBiEOG7N0C5D.
    PhylomeDBiQ99JW5.
    TreeFamiTF332767.

    Family and domain databases

    Gene3Di4.10.800.10. 1 hit.
    InterProiIPR000716. Thyroglobulin_1.
    [Graphical view]
    PfamiPF00086. Thyroglobulin_1. 1 hit.
    [Graphical view]
    SMARTiSM00211. TY. 1 hit.
    [Graphical view]
    SUPFAMiSSF57610. SSF57610. 1 hit.
    PROSITEiPS00484. THYROGLOBULIN_1_1. 1 hit.
    PS51162. THYROGLOBULIN_1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99JW5-1 [UniParc]FASTAAdd to Basket

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    MAGPQALAFG LLLAVVTATL AAAQRDCVCD NYKLATSCSL NEYGECQCTS    50
    YGTQNTVICS KLASKCLAMK AEMTHSKSGR RIKPEGAIQN NDGLYDPDCD 100
    EQGLFKAKQC NGTATCWCVN TAGVRRTDKD TEITCSERVR TYWIIIELKH 150
    KERESPYDHQ SLQTALQEAF TSRYKLNQKF IKNIMYENNV ITIDLMQNSS 200
    QKTQDDVDIA DVAYYFEKDV KGESLFHSSK SMDLRVNGEP LDLDPGQTLI 250
    YYVDEKAPEF SMQGLTAGII AVIVVVSLAV IAGIVVLVIS TRKKSAKYEK 300
    AEIKEMGEIH RELNA 315
    Length:315
    Mass (Da):35,019
    Last modified:June 1, 2001 - v1
    Checksum:iFDA853E165BA8906
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti87 – 871Missing in AAA37543. (PubMed:1729376)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76124 mRNA. Translation: AAA37543.1.
    AK145752 mRNA. Translation: BAE26627.1.
    AK167182 mRNA. Translation: BAE39317.1.
    BC005618 mRNA. Translation: AAH05618.1.
    BC094465 mRNA. Translation: AAH94465.1.
    CCDSiCCDS29018.1.
    RefSeqiNP_032558.2. NM_008532.2.
    UniGeneiMm.4259.

    Genome annotation databases

    EnsembliENSMUST00000053577; ENSMUSP00000061935; ENSMUSG00000045394.
    GeneIDi17075.
    KEGGimmu:17075.
    UCSCiuc008duy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76124 mRNA. Translation: AAA37543.1 .
    AK145752 mRNA. Translation: BAE26627.1 .
    AK167182 mRNA. Translation: BAE39317.1 .
    BC005618 mRNA. Translation: AAH05618.1 .
    BC094465 mRNA. Translation: AAH94465.1 .
    CCDSi CCDS29018.1.
    RefSeqi NP_032558.2. NM_008532.2.
    UniGenei Mm.4259.

    3D structure databases

    ProteinModelPortali Q99JW5.
    SMRi Q99JW5. Positions 93-137.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q99JW5. 3 interactions.

    Proteomic databases

    MaxQBi Q99JW5.
    PaxDbi Q99JW5.
    PRIDEi Q99JW5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000053577 ; ENSMUSP00000061935 ; ENSMUSG00000045394 .
    GeneIDi 17075.
    KEGGi mmu:17075.
    UCSCi uc008duy.1. mouse.

    Organism-specific databases

    CTDi 4072.
    MGIi MGI:106653. Epcam.

    Phylogenomic databases

    eggNOGi NOG46689.
    GeneTreei ENSGT00390000018245.
    HOGENOMi HOG000074086.
    InParanoidi Q61512.
    KOi K06737.
    OMAi MQGLQAG.
    OrthoDBi EOG7N0C5D.
    PhylomeDBi Q99JW5.
    TreeFami TF332767.

    Miscellaneous databases

    NextBioi 291194.
    PROi Q99JW5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99JW5.
    Bgeei Q99JW5.
    Genevestigatori Q99JW5.

    Family and domain databases

    Gene3Di 4.10.800.10. 1 hit.
    InterProi IPR000716. Thyroglobulin_1.
    [Graphical view ]
    Pfami PF00086. Thyroglobulin_1. 1 hit.
    [Graphical view ]
    SMARTi SM00211. TY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57610. SSF57610. 1 hit.
    PROSITEi PS00484. THYROGLOBULIN_1_1. 1 hit.
    PS51162. THYROGLOBULIN_1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A murine cDNA encodes a pan-epithelial glycoprotein that is also expressed on plasma cells."
      Bergsagel P.L., Victor-Kobrin C., Timblin C.R., Trepel J., Kuehl W.M.
      J. Immunol. 148:590-596(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiEPCAM_MOUSE
    AccessioniPrimary (citable) accession number: Q99JW5
    Secondary accession number(s): Q61512
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3