Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aminoacylase-1

Gene

Acy1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).By similarity

Catalytic activityi

An N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate.
An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi80Zinc 1By similarity1
Active sitei82By similarity1
Metal bindingi113Zinc 1By similarity1
Metal bindingi113Zinc 2By similarity1
Active sitei147Proton acceptorBy similarity1
Metal bindingi148Zinc 2By similarity1
Metal bindingi175Zinc 1By similarity1
Metal bindingi373Zinc 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.1.14 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoacylase-1 (EC:3.5.1.14)
Short name:
ACY-1
Alternative name(s):
N-acyl-L-amino-acid amidohydrolase
Gene namesi
Name:Acy1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:87913 Acy1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002740071 – 408Aminoacylase-1Add BLAST408

Proteomic databases

EPDiQ99JW2
MaxQBiQ99JW2
PaxDbiQ99JW2
PeptideAtlasiQ99JW2
PRIDEiQ99JW2

2D gel databases

REPRODUCTION-2DPAGEiQ99JW2

PTM databases

iPTMnetiQ99JW2
PhosphoSitePlusiQ99JW2

Expressioni

Gene expression databases

CleanExiMM_ACY1

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with SPHK1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi2249331 interactor.
IntActiQ99JW2 4 interactors.
MINTiQ99JW2
STRINGi10090.ENSMUSP00000024031

Structurei

3D structure databases

ProteinModelPortaliQ99JW2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated

Phylogenomic databases

eggNOGiKOG2275 Eukaryota
COG0624 LUCA
HOGENOMiHOG000021196
HOVERGENiHBG000982
InParanoidiQ99JW2
KOiK14677
PhylomeDBiQ99JW2
TreeFamiTF313693

Family and domain databases

CDDicd05646 M20_AcylaseI_like, 1 hit
InterProiView protein in InterPro
IPR001261 ArgE/DapE_CS
IPR036264 Bact_exopeptidase_dim_dom
IPR010159 N-acyl_aa_amidohydrolase
IPR002933 Peptidase_M20
IPR011650 Peptidase_M20_dimer
PfamiView protein in Pfam
PF07687 M20_dimer, 1 hit
PF01546 Peptidase_M20, 1 hit
SUPFAMiSSF55031 SSF55031, 1 hit
TIGRFAMsiTIGR01880 Ac-peptdase-euk, 1 hit
PROSITEiView protein in PROSITE
PS00758 ARGE_DAPE_CPG2_1, 1 hit
PS00759 ARGE_DAPE_CPG2_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q99JW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTKDPESEH PSVTLFRQYL RICTVQPNPD YGGAITFLEE RARQLGLSCQ
60 70 80 90 100
KIEVVPGFVI TVLTWPGTNP SLPSILLNSH TDVVPVFKEH WHHDPFEAFK
110 120 130 140 150
DSEGYIYARG SQDMKSVSIQ YLEAVRRLKS EGHRFPRTIH MTFVPDEEVG
160 170 180 190 200
GHKGMELFVK RPEFQALRAG FALDEGLANP TDAFTVFYSE RSPWWVRVTS
210 220 230 240 250
TGKPGHASRF IEDTAAEKLH KVISSILAFR EKERQRLQAN PHLKEGAVTS
260 270 280 290 300
VNLTKLEGGV AYNVVPATMS ASFDFRVAPD VDMKAFEKQL QRWCQEAGEG
310 320 330 340 350
VTFEFAQKFT EPRMTPTDDS DPWWAAFSGA CKAMNLTLEP EIFPAATDSR
360 370 380 390 400
YIRAVGIPAL GFSPMNRTPV LLHDHNERLH EDIFLRGVDI YTGLLSALAS

VPTLPGES
Length:408
Mass (Da):45,781
Last modified:June 1, 2001 - v1
Checksum:i384C70B9AB87CDC3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti172A → V in BAB22090 (PubMed:16141072).Curated1
Sequence conflicti172A → V in BAB22948 (PubMed:16141072).Curated1
Sequence conflicti197R → Q in BAB22090 (PubMed:16141072).Curated1
Sequence conflicti197R → Q in BAB22948 (PubMed:16141072).Curated1
Sequence conflicti351Y → F in BAB22090 (PubMed:16141072).Curated1
Sequence conflicti351Y → F in BAB22948 (PubMed:16141072).Curated1
Sequence conflicti406G → S in BAB22090 (PubMed:16141072).Curated1
Sequence conflicti406G → S in BAB22948 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003703 mRNA Translation: BAB22948.1
AK002423 mRNA Translation: BAB22090.1
BC005631 mRNA Translation: AAH05631.1
CCDSiCCDS23477.1
RefSeqiNP_001263371.1, NM_001276442.1
NP_079647.1, NM_025371.3
UniGeneiMm.7165

Genome annotation databases

GeneIDi109652
KEGGimmu:109652
UCSCiuc009rjp.2 mouse

Similar proteinsi

Entry informationi

Entry nameiACY1_MOUSE
AccessioniPrimary (citable) accession number: Q99JW2
Secondary accession number(s): Q9CR15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome