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Q99JW2

- ACY1_MOUSE

UniProt

Q99JW2 - ACY1_MOUSE

Protein

Aminoacylase-1

Gene

Acy1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).By similarity

    Catalytic activityi

    An N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate.
    An N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi80 – 801Zinc 1By similarity
    Active sitei82 – 821By similarity
    Metal bindingi113 – 1131Zinc 1By similarity
    Metal bindingi113 – 1131Zinc 2By similarity
    Active sitei147 – 1471Proton acceptorBy similarity
    Metal bindingi148 – 1481Zinc 2By similarity
    Metal bindingi175 – 1751Zinc 1By similarity
    Metal bindingi373 – 3731Zinc 2By similarity

    GO - Molecular functioni

    1. aminoacylase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. metallopeptidase activity Source: InterPro

    GO - Biological processi

    1. cellular amino acid metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.5.1.14. 3474.

    Protein family/group databases

    MEROPSiM20.973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminoacylase-1 (EC:3.5.1.14)
    Short name:
    ACY-1
    Alternative name(s):
    N-acyl-L-amino-acid amidohydrolase
    Gene namesi
    Name:Acy1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:87913. Acy1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 408407Aminoacylase-1PRO_0000274007Add
    BLAST

    Proteomic databases

    MaxQBiQ99JW2.
    PaxDbiQ99JW2.
    PRIDEiQ99JW2.

    2D gel databases

    REPRODUCTION-2DPAGEQ99JW2.

    PTM databases

    PhosphoSiteiQ99JW2.

    Expressioni

    Gene expression databases

    BgeeiQ99JW2.
    CleanExiMM_ACY1.
    GenevestigatoriQ99JW2.

    Interactioni

    Subunit structurei

    Homodimer By similarity. Interacts with SPHK1.By similarity1 Publication

    Protein-protein interaction databases

    IntActiQ99JW2. 3 interactions.
    MINTiMINT-1856196.
    STRINGi10090.ENSMUSP00000024031.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99JW2.
    SMRiQ99JW2. Positions 7-405.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M20A family.Curated

    Phylogenomic databases

    eggNOGiCOG0624.
    GeneTreeiENSGT00730000111049.
    HOGENOMiHOG000021196.
    HOVERGENiHBG000982.
    InParanoidiQ99JW2.
    KOiK14677.
    OrthoDBiEOG7N8ZVZ.
    PhylomeDBiQ99JW2.
    TreeFamiTF313693.

    Family and domain databases

    Gene3Di3.30.70.360. 1 hit.
    InterProiIPR001261. ArgE/DapE_CS.
    IPR010159. N-acyl_aa_amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view]
    PfamiPF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036696. ACY-1. 1 hit.
    SUPFAMiSSF55031. SSF55031. 1 hit.
    TIGRFAMsiTIGR01880. Ac-peptdase-euk. 1 hit.
    PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
    PS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99JW2-1 [UniParc]FASTAAdd to Basket

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    MTTKDPESEH PSVTLFRQYL RICTVQPNPD YGGAITFLEE RARQLGLSCQ    50
    KIEVVPGFVI TVLTWPGTNP SLPSILLNSH TDVVPVFKEH WHHDPFEAFK 100
    DSEGYIYARG SQDMKSVSIQ YLEAVRRLKS EGHRFPRTIH MTFVPDEEVG 150
    GHKGMELFVK RPEFQALRAG FALDEGLANP TDAFTVFYSE RSPWWVRVTS 200
    TGKPGHASRF IEDTAAEKLH KVISSILAFR EKERQRLQAN PHLKEGAVTS 250
    VNLTKLEGGV AYNVVPATMS ASFDFRVAPD VDMKAFEKQL QRWCQEAGEG 300
    VTFEFAQKFT EPRMTPTDDS DPWWAAFSGA CKAMNLTLEP EIFPAATDSR 350
    YIRAVGIPAL GFSPMNRTPV LLHDHNERLH EDIFLRGVDI YTGLLSALAS 400
    VPTLPGES 408
    Length:408
    Mass (Da):45,781
    Last modified:June 1, 2001 - v1
    Checksum:i384C70B9AB87CDC3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti172 – 1721A → V in BAB22090. (PubMed:16141072)Curated
    Sequence conflicti172 – 1721A → V in BAB22948. (PubMed:16141072)Curated
    Sequence conflicti197 – 1971R → Q in BAB22090. (PubMed:16141072)Curated
    Sequence conflicti197 – 1971R → Q in BAB22948. (PubMed:16141072)Curated
    Sequence conflicti351 – 3511Y → F in BAB22090. (PubMed:16141072)Curated
    Sequence conflicti351 – 3511Y → F in BAB22948. (PubMed:16141072)Curated
    Sequence conflicti406 – 4061G → S in BAB22090. (PubMed:16141072)Curated
    Sequence conflicti406 – 4061G → S in BAB22948. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK003703 mRNA. Translation: BAB22948.1.
    AK002423 mRNA. Translation: BAB22090.1.
    BC005631 mRNA. Translation: AAH05631.1.
    CCDSiCCDS23477.1.
    RefSeqiNP_001263371.1. NM_001276442.1.
    NP_079647.1. NM_025371.3.
    UniGeneiMm.7165.

    Genome annotation databases

    EnsembliENSMUST00000024031; ENSMUSP00000024031; ENSMUSG00000023262.
    GeneIDi109652.
    KEGGimmu:109652.
    UCSCiuc009rjp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK003703 mRNA. Translation: BAB22948.1 .
    AK002423 mRNA. Translation: BAB22090.1 .
    BC005631 mRNA. Translation: AAH05631.1 .
    CCDSi CCDS23477.1.
    RefSeqi NP_001263371.1. NM_001276442.1.
    NP_079647.1. NM_025371.3.
    UniGenei Mm.7165.

    3D structure databases

    ProteinModelPortali Q99JW2.
    SMRi Q99JW2. Positions 7-405.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q99JW2. 3 interactions.
    MINTi MINT-1856196.
    STRINGi 10090.ENSMUSP00000024031.

    Protein family/group databases

    MEROPSi M20.973.

    PTM databases

    PhosphoSitei Q99JW2.

    2D gel databases

    REPRODUCTION-2DPAGE Q99JW2.

    Proteomic databases

    MaxQBi Q99JW2.
    PaxDbi Q99JW2.
    PRIDEi Q99JW2.

    Protocols and materials databases

    DNASUi 109652.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024031 ; ENSMUSP00000024031 ; ENSMUSG00000023262 .
    GeneIDi 109652.
    KEGGi mmu:109652.
    UCSCi uc009rjp.1. mouse.

    Organism-specific databases

    CTDi 95.
    MGIi MGI:87913. Acy1.

    Phylogenomic databases

    eggNOGi COG0624.
    GeneTreei ENSGT00730000111049.
    HOGENOMi HOG000021196.
    HOVERGENi HBG000982.
    InParanoidi Q99JW2.
    KOi K14677.
    OrthoDBi EOG7N8ZVZ.
    PhylomeDBi Q99JW2.
    TreeFami TF313693.

    Enzyme and pathway databases

    BRENDAi 3.5.1.14. 3474.

    Miscellaneous databases

    NextBioi 362511.
    PROi Q99JW2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q99JW2.
    CleanExi MM_ACY1.
    Genevestigatori Q99JW2.

    Family and domain databases

    Gene3Di 3.30.70.360. 1 hit.
    InterProi IPR001261. ArgE/DapE_CS.
    IPR010159. N-acyl_aa_amidohydrolase.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view ]
    Pfami PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036696. ACY-1. 1 hit.
    SUPFAMi SSF55031. SSF55031. 1 hit.
    TIGRFAMsi TIGR01880. Ac-peptdase-euk. 1 hit.
    PROSITEi PS00758. ARGE_DAPE_CPG2_1. 1 hit.
    PS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo and Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. "Aminoacylase 1 is a sphingosine kinase 1-interacting protein."
      Maceyka M., Nava V.E., Milstien S., Spiegel S.
      FEBS Lett. 568:30-34(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SPHK1.

    Entry informationi

    Entry nameiACY1_MOUSE
    AccessioniPrimary (citable) accession number: Q99JW2
    Secondary accession number(s): Q9CR15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3