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Q99JT6 (TLCD1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calfacilitin
Alternative name(s):
TLC domain-containing protein 1
Gene names
Name:Tlcd1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium channel facilitator that increases calcium flux by generating a larger window current and slowing inactivation of the L-type CACNA1C/CaV1.2 channel. Regulation of intracellular calcium by Calfacilitin is required for neural plate formation By similarity.

Subunit structure

Interacts with CACNA1C By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Contains 1 TLC (TRAM/LAG1/CLN8) domain.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   LigandCalcium
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99JT6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99JT6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     170-196: Missing.
Isoform 3 (identifier: Q99JT6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: MPLLFHPAWP...IVSGIWALLC → MRWPWDLSRG...QPRTAVGNPS
Isoform 4 (identifier: Q99JT6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     121-127: AMGAFFS → VRENPVK
     128-247: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 247220Calfacilitin
PRO_0000285678

Regions

Topological domain28 – 4619Extracellular Potential
Transmembrane47 – 6721Helical; Potential
Topological domain68 – 8316Cytoplasmic Potential
Transmembrane84 – 10421Helical; Potential
Topological domain105 – 12319Extracellular Potential
Intramembrane124 – 14421Helical; Potential
Topological domain145 – 17329Extracellular Potential
Transmembrane174 – 19421Helical; Potential
Topological domain195 – 2017Cytoplasmic Potential
Transmembrane202 – 22221Helical; Potential
Topological domain223 – 24725Extracellular Potential
Domain40 – 234195TLC

Natural variations

Alternative sequence1 – 6565MPLLF…WALLC → MRWPWDLSRGARTGLPLAIP ITGEQTPRISPDSVWKTTQP RTAVGNPS in isoform 3.
VSP_024887
Alternative sequence121 – 1277AMGAFFS → VRENPVK in isoform 4.
VSP_024888
Alternative sequence128 – 247120Missing in isoform 4.
VSP_024889
Alternative sequence170 – 19627Missing in isoform 2.
VSP_024890

Experimental info

Sequence conflict281R → H in BAE20654. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 84AEA3CBE8BE4093

FASTA24728,787
        10         20         30         40         50         60 
MPLLFHPAWP LLLGATLTFR ALRRVLCRLP QPAHVQTDPL RTWRWHNLLV SFTHSIVSGI 

        70         80         90        100        110        120 
WALLCLWQTP EMLVEIETAW SASGYLLVCF SAGYFIHDTV DIVVSKQTRA SWEYLVHHVM 

       130        140        150        160        170        180 
AMGAFFSGIF WKRFVGGGVL TLLVEVSNIF LTLRMMMKIN NAQDLLLYKV NKYINLVMYF 

       190        200        210        220        230        240 
LFRLAPQAYL TKFFLQYAGQ RTLGTFLLAI LLMLDLMIII YFSRLLRSDF CPERAPRRQQ 


KDKFLTE 

« Hide

Isoform 2 [UniParc].

Checksum: 4B79D9A43DEEFF4F
Show »

FASTA22025,463
Isoform 3 [UniParc].

Checksum: 4C6FA50D091D0D29
Show »

FASTA23026,548
Isoform 4 [UniParc].

Checksum: A8E256D59F585165
Show »

FASTA12714,703

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
Strain: C57BL/6J.
Tissue: Adipose tissue, Kidney and Thymus.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Strain: Czech II and FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK002703 mRNA. Translation: BAB22297.1.
AK046577 mRNA. Translation: BAE20654.1.
AK161721 mRNA. Translation: BAE36548.1.
AK165432 mRNA. Translation: BAE38185.1.
AL591070 Genomic DNA. Translation: CAI24331.1.
AL591070 Genomic DNA. Translation: CAI24332.1.
AL591070 Genomic DNA. Translation: CAI24333.1.
AL591070 Genomic DNA. Translation: CAI24334.1.
BC005702 mRNA. Translation: AAH05702.1.
BC039182 mRNA. Translation: AAH39182.1.
CCDSCCDS25092.1. [Q99JT6-1]
RefSeqNP_001278165.1. NM_001291236.1. [Q99JT6-3]
NP_001278166.1. NM_001291237.1. [Q99JT6-3]
NP_080984.1. NM_026708.2. [Q99JT6-1]
XP_006534117.1. XM_006534054.1. [Q99JT6-1]
UniGeneMm.390375.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ99JT6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000092880; ENSMUSP00000090556; ENSMUSG00000019437. [Q99JT6-3]
ENSMUST00000098545; ENSMUSP00000096145; ENSMUSG00000019437. [Q99JT6-2]
ENSMUST00000108338; ENSMUSP00000103975; ENSMUSG00000019437. [Q99JT6-4]
ENSMUST00000127587; ENSMUSP00000114202; ENSMUSG00000019437. [Q99JT6-1]
GeneID68385.
KEGGmmu:68385.
UCSCuc007kij.1. mouse. [Q99JT6-1]
uc007kik.1. mouse. [Q99JT6-3]

Organism-specific databases

CTD116238.
MGIMGI:1915572. Tlcd1.

Phylogenomic databases

eggNOGNOG258821.
GeneTreeENSGT00520000055611.
HOGENOMHOG000015396.
HOVERGENHBG100100.
InParanoidQ99JT6.
OMACVERNIS.
OrthoDBEOG725DJM.
PhylomeDBQ99JT6.
TreeFamTF315115.

Gene expression databases

ArrayExpressQ99JT6.
BgeeQ99JT6.
CleanExMM_TLCD1.
GenevestigatorQ99JT6.

Family and domain databases

InterProIPR006634. TLC-dom.
[Graphical view]
PfamPF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
SMARTSM00724. TLC. 1 hit.
[Graphical view]
PROSITEPS50922. TLC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio327098.
PROQ99JT6.
SOURCESearch...

Entry information

Entry nameTLCD1_MOUSE
AccessionPrimary (citable) accession number: Q99JT6
Secondary accession number(s): Q3V372 expand/collapse secondary AC list , Q5SYM9, Q8CHT9, Q9DCK5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot