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Protein

Calfacilitin

Gene

Tlcd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium channel facilitator that increases calcium flux by generating a larger window current and slowing inactivation of the L-type CACNA1C/CaV1.2 channel. Regulation of intracellular calcium by Calfacilitin is required for neural plate formation (By similarity).By similarity

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Calfacilitin
Alternative name(s):
TLC domain-containing protein 1
Gene namesi
Name:Tlcd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1915572. Tlcd1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 4619ExtracellularSequence AnalysisAdd
BLAST
Transmembranei47 – 6721HelicalSequence AnalysisAdd
BLAST
Topological domaini68 – 8316CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei84 – 10421HelicalSequence AnalysisAdd
BLAST
Topological domaini105 – 12319ExtracellularSequence AnalysisAdd
BLAST
Intramembranei124 – 14421HelicalSequence AnalysisAdd
BLAST
Topological domaini145 – 17329ExtracellularSequence AnalysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence AnalysisAdd
BLAST
Topological domaini195 – 2017CytoplasmicSequence Analysis
Transmembranei202 – 22221HelicalSequence AnalysisAdd
BLAST
Topological domaini223 – 24725ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 247220CalfacilitinPRO_0000285678Add
BLAST

Proteomic databases

PRIDEiQ99JT6.

Expressioni

Gene expression databases

BgeeiQ99JT6.
CleanExiMM_TLCD1.
ExpressionAtlasiQ99JT6. baseline and differential.
GenevisibleiQ99JT6. MM.

Interactioni

Subunit structurei

Interacts with CACNA1C.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000114202.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 234195TLCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG258821.
GeneTreeiENSGT00520000055611.
HOGENOMiHOG000015396.
HOVERGENiHBG100100.
InParanoidiQ99JT6.
OMAiCVWQTPE.
OrthoDBiEOG725DJM.
PhylomeDBiQ99JT6.
TreeFamiTF315115.

Family and domain databases

InterProiIPR006634. TLC-dom.
[Graphical view]
PfamiPF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99JT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLLFHPAWP LLLGATLTFR ALRRVLCRLP QPAHVQTDPL RTWRWHNLLV
60 70 80 90 100
SFTHSIVSGI WALLCLWQTP EMLVEIETAW SASGYLLVCF SAGYFIHDTV
110 120 130 140 150
DIVVSKQTRA SWEYLVHHVM AMGAFFSGIF WKRFVGGGVL TLLVEVSNIF
160 170 180 190 200
LTLRMMMKIN NAQDLLLYKV NKYINLVMYF LFRLAPQAYL TKFFLQYAGQ
210 220 230 240
RTLGTFLLAI LLMLDLMIII YFSRLLRSDF CPERAPRRQQ KDKFLTE
Length:247
Mass (Da):28,787
Last modified:June 1, 2001 - v1
Checksum:i84AEA3CBE8BE4093
GO
Isoform 2 (identifier: Q99JT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     170-196: Missing.

Show »
Length:220
Mass (Da):25,463
Checksum:i4B79D9A43DEEFF4F
GO
Isoform 3 (identifier: Q99JT6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: MPLLFHPAWP...IVSGIWALLC → MRWPWDLSRG...QPRTAVGNPS

Show »
Length:230
Mass (Da):26,548
Checksum:i4C6FA50D091D0D29
GO
Isoform 4 (identifier: Q99JT6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     121-127: AMGAFFS → VRENPVK
     128-247: Missing.

Show »
Length:127
Mass (Da):14,703
Checksum:iA8E256D59F585165
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281R → H in BAE20654 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6565MPLLF…WALLC → MRWPWDLSRGARTGLPLAIP ITGEQTPRISPDSVWKTTQP RTAVGNPS in isoform 3. 2 PublicationsVSP_024887Add
BLAST
Alternative sequencei121 – 1277AMGAFFS → VRENPVK in isoform 4. 1 PublicationVSP_024888
Alternative sequencei128 – 247120Missing in isoform 4. 1 PublicationVSP_024889Add
BLAST
Alternative sequencei170 – 19627Missing in isoform 2. 1 PublicationVSP_024890Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002703 mRNA. Translation: BAB22297.1.
AK046577 mRNA. Translation: BAE20654.1.
AK161721 mRNA. Translation: BAE36548.1.
AK165432 mRNA. Translation: BAE38185.1.
AL591070 Genomic DNA. Translation: CAI24331.1.
AL591070 Genomic DNA. Translation: CAI24332.1.
AL591070 Genomic DNA. Translation: CAI24333.1.
AL591070 Genomic DNA. Translation: CAI24334.1.
BC005702 mRNA. Translation: AAH05702.1.
BC039182 mRNA. Translation: AAH39182.1.
CCDSiCCDS25092.1. [Q99JT6-1]
CCDS70257.1. [Q99JT6-3]
RefSeqiNP_001278165.1. NM_001291236.1. [Q99JT6-3]
NP_001278166.1. NM_001291237.1. [Q99JT6-3]
NP_080984.1. NM_026708.2. [Q99JT6-1]
XP_006534117.1. XM_006534054.1. [Q99JT6-1]
UniGeneiMm.390375.

Genome annotation databases

EnsembliENSMUST00000092880; ENSMUSP00000090556; ENSMUSG00000019437. [Q99JT6-3]
ENSMUST00000098545; ENSMUSP00000096145; ENSMUSG00000019437. [Q99JT6-2]
ENSMUST00000108338; ENSMUSP00000103975; ENSMUSG00000019437. [Q99JT6-4]
ENSMUST00000127587; ENSMUSP00000114202; ENSMUSG00000019437. [Q99JT6-1]
GeneIDi68385.
UCSCiuc007kij.2. mouse. [Q99JT6-1]
uc007kik.2. mouse. [Q99JT6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002703 mRNA. Translation: BAB22297.1.
AK046577 mRNA. Translation: BAE20654.1.
AK161721 mRNA. Translation: BAE36548.1.
AK165432 mRNA. Translation: BAE38185.1.
AL591070 Genomic DNA. Translation: CAI24331.1.
AL591070 Genomic DNA. Translation: CAI24332.1.
AL591070 Genomic DNA. Translation: CAI24333.1.
AL591070 Genomic DNA. Translation: CAI24334.1.
BC005702 mRNA. Translation: AAH05702.1.
BC039182 mRNA. Translation: AAH39182.1.
CCDSiCCDS25092.1. [Q99JT6-1]
CCDS70257.1. [Q99JT6-3]
RefSeqiNP_001278165.1. NM_001291236.1. [Q99JT6-3]
NP_001278166.1. NM_001291237.1. [Q99JT6-3]
NP_080984.1. NM_026708.2. [Q99JT6-1]
XP_006534117.1. XM_006534054.1. [Q99JT6-1]
UniGeneiMm.390375.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000114202.

Proteomic databases

PRIDEiQ99JT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092880; ENSMUSP00000090556; ENSMUSG00000019437. [Q99JT6-3]
ENSMUST00000098545; ENSMUSP00000096145; ENSMUSG00000019437. [Q99JT6-2]
ENSMUST00000108338; ENSMUSP00000103975; ENSMUSG00000019437. [Q99JT6-4]
ENSMUST00000127587; ENSMUSP00000114202; ENSMUSG00000019437. [Q99JT6-1]
GeneIDi68385.
UCSCiuc007kij.2. mouse. [Q99JT6-1]
uc007kik.2. mouse. [Q99JT6-3]

Organism-specific databases

CTDi116238.
MGIiMGI:1915572. Tlcd1.

Phylogenomic databases

eggNOGiNOG258821.
GeneTreeiENSGT00520000055611.
HOGENOMiHOG000015396.
HOVERGENiHBG100100.
InParanoidiQ99JT6.
OMAiCVWQTPE.
OrthoDBiEOG725DJM.
PhylomeDBiQ99JT6.
TreeFamiTF315115.

Miscellaneous databases

NextBioi327098.
PROiQ99JT6.
SOURCEiSearch...

Gene expression databases

BgeeiQ99JT6.
CleanExiMM_TLCD1.
ExpressionAtlasiQ99JT6. baseline and differential.
GenevisibleiQ99JT6. MM.

Family and domain databases

InterProiIPR006634. TLC-dom.
[Graphical view]
PfamiPF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
    Strain: C57BL/6J.
    Tissue: Adipose tissue, Kidney and Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiTLCD1_MOUSE
AccessioniPrimary (citable) accession number: Q99JT6
Secondary accession number(s): Q3V372
, Q5SYM9, Q8CHT9, Q9DCK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2001
Last modified: July 22, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.