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Protein

Sideroflexin-1

Gene

Sfxn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Might be involved in the transport of a component required for iron utilization into or out of the mitochondria.

GO - Molecular functioni

GO - Biological processi

  • erythrocyte differentiation Source: MGI
  • iron ion homeostasis Source: UniProtKB-KW
  • iron ion transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Names & Taxonomyi

Protein namesi
Recommended name:
Sideroflexin-1
Gene namesi
Name:Sfxn1
Synonyms:F
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2137677. Sfxn1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei103 – 12018HelicalSequence analysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence analysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence analysisAdd
BLAST
Transmembranei229 – 24921HelicalSequence analysisAdd
BLAST
Transmembranei267 – 28721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Involvement in diseasei

Defects in Sfxn1 are the cause of a transitory hypochromic, microcytic anemia characterized by a large number of siderocytes containing non-heme iron granules. The anemia begins at 12 dpc, is most intense at 15 dpc and is still severe at birth, but disappears by 2 weeks of age. Mutant adults are no longer anemic, but they have an impaired response to hemopoietic stress. Most homozygotes also have flexed tails and a belly spot.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 322321Sideroflexin-1PRO_0000177033Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ99JR1.
MaxQBiQ99JR1.
PaxDbiQ99JR1.
PRIDEiQ99JR1.

PTM databases

iPTMnetiQ99JR1.
PhosphoSiteiQ99JR1.
SwissPalmiQ99JR1.

Expressioni

Tissue specificityi

Widely expressed, with highest expression in kidney and liver.1 Publication

Developmental stagei

Very high levels in the liver during the period of embryonic hepatic hemopoiesis.

Gene expression databases

BgeeiQ99JR1.
CleanExiMM_SFXN1.
GenevisibleiQ99JR1. MM.

Interactioni

Protein-protein interaction databases

BioGridi199565. 1 interaction.
IntActiQ99JR1. 4 interactions.
MINTiMINT-4997949.
STRINGi10090.ENSMUSP00000021930.

Structurei

3D structure databases

ProteinModelPortaliQ99JR1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sideroflexin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3767. Eukaryota.
ENOG410YU8V. LUCA.
GeneTreeiENSGT00390000002026.
HOGENOMiHOG000203440.
HOVERGENiHBG056083.
InParanoidiQ99JR1.
OMAiKVVHDYR.
OrthoDBiEOG70KGPJ.
PhylomeDBiQ99JR1.
TreeFamiTF313205.

Family and domain databases

InterProiIPR004686. Mtc.
[Graphical view]
PANTHERiPTHR11153. PTHR11153. 1 hit.
PfamiPF03820. Mtc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00798. mtc. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99JR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGEVPPNIN IKEPRWDQST FIGRASHFFT VTDPRNILLT NEQLENARKV
60 70 80 90 100
VHDYRQGIVP AGLTENELWR AKYAYDSAFH PDTGEKMTLI GRMSAQVPMN
110 120 130 140 150
MTITGCMMTF YRTTPAVLFW QWINQSFNAV VNYTNRSGDA PLTVNELGTA
160 170 180 190 200
YVSATTGAVA TALGLNALTK RVSPLIGRFV PFAAVAAANC INIPLMRQRE
210 220 230 240 250
LKVGIPVTDE NGTRLGESTN AAKQAITQVV ISRILMAAPG MAIPPFIMNT
260 270 280 290 300
LEKKAFLKRF PWMSAPIQVT LVGFCLVFAT PLCCALFPQK SSMSVTSLED
310 320
ELQASIQRTH PEIRRVYFNK GL
Length:322
Mass (Da):35,649
Last modified:January 23, 2007 - v3
Checksum:iE3B055CB03CEDFA7
GO

Sequence cautioni

The sequence AK012650 differs from that shown. Reason: Frameshift at position 283. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF325260 mRNA. Translation: AAK39428.1.
AK012650 mRNA. No translation available.
AK151110 mRNA. Translation: BAE30120.1.
BC005743 mRNA. Translation: AAH05743.1.
CCDSiCCDS26525.1.
RefSeqiNP_081600.1. NM_027324.5.
XP_006516912.2. XM_006516849.2.
XP_006516913.1. XM_006516850.2.
UniGeneiMm.134191.

Genome annotation databases

EnsembliENSMUST00000021930; ENSMUSP00000021930; ENSMUSG00000021474.
GeneIDi14057.
KEGGimmu:14057.
UCSCiuc007qoa.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF325260 mRNA. Translation: AAK39428.1.
AK012650 mRNA. No translation available.
AK151110 mRNA. Translation: BAE30120.1.
BC005743 mRNA. Translation: AAH05743.1.
CCDSiCCDS26525.1.
RefSeqiNP_081600.1. NM_027324.5.
XP_006516912.2. XM_006516849.2.
XP_006516913.1. XM_006516850.2.
UniGeneiMm.134191.

3D structure databases

ProteinModelPortaliQ99JR1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199565. 1 interaction.
IntActiQ99JR1. 4 interactions.
MINTiMINT-4997949.
STRINGi10090.ENSMUSP00000021930.

PTM databases

iPTMnetiQ99JR1.
PhosphoSiteiQ99JR1.
SwissPalmiQ99JR1.

Proteomic databases

EPDiQ99JR1.
MaxQBiQ99JR1.
PaxDbiQ99JR1.
PRIDEiQ99JR1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021930; ENSMUSP00000021930; ENSMUSG00000021474.
GeneIDi14057.
KEGGimmu:14057.
UCSCiuc007qoa.2. mouse.

Organism-specific databases

CTDi94081.
MGIiMGI:2137677. Sfxn1.

Phylogenomic databases

eggNOGiKOG3767. Eukaryota.
ENOG410YU8V. LUCA.
GeneTreeiENSGT00390000002026.
HOGENOMiHOG000203440.
HOVERGENiHBG056083.
InParanoidiQ99JR1.
OMAiKVVHDYR.
OrthoDBiEOG70KGPJ.
PhylomeDBiQ99JR1.
TreeFamiTF313205.

Miscellaneous databases

ChiTaRSiSfxn1. mouse.
NextBioi285016.
PROiQ99JR1.
SOURCEiSearch...

Gene expression databases

BgeeiQ99JR1.
CleanExiMM_SFXN1.
GenevisibleiQ99JR1. MM.

Family and domain databases

InterProiIPR004686. Mtc.
[Graphical view]
PANTHERiPTHR11153. PTHR11153. 1 hit.
PfamiPF03820. Mtc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00798. mtc. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A mutation in a mitochondrial transmembrane protein is responsible for the pleiotropic hematological and skeletal phenotype of flexed-tail (f/f) mice."
    Fleming M.D., Campagna D.R., Haslett J.N., Trenor C.C. III, Andrews N.C.
    Genes Dev. 15:652-657(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DISEASE.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow and Embryo.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 16-24; 73-86; 179-197; 203-214 AND 224-233, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiSFXN1_MOUSE
AccessioniPrimary (citable) accession number: Q99JR1
Secondary accession number(s): Q3UB44, Q9CZG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: January 23, 2007
Last modified: March 16, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.