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Protein

Mitogen-activated protein kinase kinase kinase kinase 3

Gene

Map4k3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 30ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase kinase 3 (EC:2.7.11.1)
Alternative name(s):
Germinal center kinase-related protein kinase
Short name:
GLK
MAPK/ERK kinase kinase kinase 3
Short name:
MEK kinase kinase 3
Short name:
MEKKK 3
Gene namesi
Name:Map4k3
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2154405. Map4k3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000862781 – 894Mitogen-activated protein kinase kinase kinase kinase 3Add BLAST894

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei329PhosphoserineCombined sources1
Modified residuei398PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ99JP0.
PaxDbiQ99JP0.
PeptideAtlasiQ99JP0.
PRIDEiQ99JP0.

PTM databases

iPTMnetiQ99JP0.
PhosphoSitePlusiQ99JP0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024242.
ExpressionAtlasiQ99JP0. baseline and differential.
GenevisibleiQ99JP0. MM.

Interactioni

Subunit structurei

Interacts with SH3GL2. Interaction appears to regulate MAP4K3-mediated JNK activation (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
LCP2Q130942EBI-5324222,EBI-346946From a different organism.
PrkcqQ021112EBI-5324222,EBI-2639157

Protein-protein interaction databases

IntActiQ99JP0. 3 interactors.
STRINGi10090.ENSMUSP00000108008.

Structurei

3D structure databases

ProteinModelPortaliQ99JP0.
SMRiQ99JP0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 273Protein kinasePROSITE-ProRule annotationAdd BLAST258
Domaini556 – 867CNHPROSITE-ProRule annotationAdd BLAST312

Sequence similaritiesi

Contains 1 CNH domain.PROSITE-ProRule annotationCurated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0576. Eukaryota.
ENOG410XP9G. LUCA.
GeneTreeiENSGT00810000125351.
HOGENOMiHOG000230824.
HOVERGENiHBG036702.
InParanoidiQ99JP0.
KOiK04406.

Family and domain databases

InterProiIPR001180. CNH_dom.
IPR011009. Kinase-like_dom.
IPR021160. MAPKKKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF038172. MAPKKKK. 1 hit.
SMARTiSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99JP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPGFDLSRR NPQEDFELIQ RIGSGTYGDV YKARNVNTGE LAAIKVIKLE
60 70 80 90 100
PGEDFAVVQQ EIIMMKDCKH PNIVAYFGSY LRRDKLWICM EFCGGGSLQD
110 120 130 140 150
IYHVTGPLSE LQIAYVSRET LQGLYYLHSK GKMHRDIKGA NILLTDNGHV
160 170 180 190 200
KLADFGVSAQ ITATIAKRKS FIGTPYWMAP EVAAVERKGG YNQLCDLWAV
210 220 230 240 250
GITAIELAEL QPPMFDLHPM RALFLMTKSN FQPPKLKDKL KWSNSFHHFV
260 270 280 290 300
KMALTKNPKK RPNAEKLLQH PFVTQPLTRS LAIELLDKVN NPDHSTYHDF
310 320 330 340 350
DDDDPEPLVA VPHRIPSTSR NVREEKTRSE INFGQVKFDP PLRKETEPHH
360 370 380 390 400
ELPDSDGFFD SSEEIYYTAR SNLDLQLEYG QGHQSHCFLG GNKSLLKSVE
410 420 430 440 450
EELHQRGHVA HLEDDEGDDD DSKHSTMKAK VPPPLPPKPK SIFIPQDTHS
460 470 480 490 500
AEDGNQGTIK RCPSSGSPAK PSHVPPRPPP PRLPPQKPAV LGNGVNSFQL
510 520 530 540 550
NGERDGSLYQ QQSEQRGTNL SRKEKKDVPK PISNGLPPTP KVHMGACFSK
560 570 580 590 600
VFNGCPLKIH CATSWINPDT RDQYLIFGAE EGIYTLNLNE LHETSMEQLF
610 620 630 640 650
PRRCTWLYVM NNCLLSVSGK ASQLYSHNLP GLFDYARQMQ KLPVAIPAHK
660 670 680 690 700
LPDRILPRKF AVSAKIPETK WCQKCCVVRN PYTGHKYLCG ALQTSIVLLE
710 720 730 740 750
WVEPMQKFML IKHIEFPMPC PLRMFEMLVV PEQEYPLVCV GVSRGRDFNQ
760 770 780 790 800
VVRFETVNPN STSSWFTESD APQTSVTHVT QLERDTILVC LDCCIKIVNL
810 820 830 840 850
QGRLKSSRKL SSELTFDFQI ESIVCLQDSV LAFWKHGMQG RSFRSNEVTQ
860 870 880 890
EISDNTRIFR LLGSDRVVVL ESRPTDNPTA NSNLYILAGH ENSY
Length:894
Mass (Da):101,119
Last modified:July 27, 2011 - v4
Checksum:iA9D9086ADC9DDA4F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti874P → R in AAH05781 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC131712 Genomic DNA. No translation available.
AC169501 Genomic DNA. No translation available.
AK014711 mRNA. Translation: BAB29516.1.
BC005781 mRNA. Translation: AAH05781.1.
CCDSiCCDS70847.1.
RefSeqiNP_001277274.1. NM_001290345.1.
UniGeneiMm.311948.

Genome annotation databases

EnsembliENSMUST00000025089; ENSMUSP00000025089; ENSMUSG00000024242.
GeneIDi225028.
KEGGimmu:225028.
UCSCiuc008drj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC131712 Genomic DNA. No translation available.
AC169501 Genomic DNA. No translation available.
AK014711 mRNA. Translation: BAB29516.1.
BC005781 mRNA. Translation: AAH05781.1.
CCDSiCCDS70847.1.
RefSeqiNP_001277274.1. NM_001290345.1.
UniGeneiMm.311948.

3D structure databases

ProteinModelPortaliQ99JP0.
SMRiQ99JP0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99JP0. 3 interactors.
STRINGi10090.ENSMUSP00000108008.

PTM databases

iPTMnetiQ99JP0.
PhosphoSitePlusiQ99JP0.

Proteomic databases

MaxQBiQ99JP0.
PaxDbiQ99JP0.
PeptideAtlasiQ99JP0.
PRIDEiQ99JP0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025089; ENSMUSP00000025089; ENSMUSG00000024242.
GeneIDi225028.
KEGGimmu:225028.
UCSCiuc008drj.2. mouse.

Organism-specific databases

CTDi8491.
MGIiMGI:2154405. Map4k3.

Phylogenomic databases

eggNOGiKOG0576. Eukaryota.
ENOG410XP9G. LUCA.
GeneTreeiENSGT00810000125351.
HOGENOMiHOG000230824.
HOVERGENiHBG036702.
InParanoidiQ99JP0.
KOiK04406.

Miscellaneous databases

PROiQ99JP0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024242.
ExpressionAtlasiQ99JP0. baseline and differential.
GenevisibleiQ99JP0. MM.

Family and domain databases

InterProiIPR001180. CNH_dom.
IPR011009. Kinase-like_dom.
IPR021160. MAPKKKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF038172. MAPKKKK. 1 hit.
SMARTiSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiM4K3_MOUSE
AccessioniPrimary (citable) accession number: Q99JP0
Secondary accession number(s): E9PWI4, Q9CUS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 134 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.