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Protein

Kelch-like protein 22

Gene

Klhl22

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Kelch-like protein 22
Gene namesi
Name:Klhl22
Synonyms:Kelchl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1337995. Klhl22.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Cytoplasmcytoskeletonspindle By similarity

  • Note: Mainly. cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 634633Kelch-like protein 22PRO_0000242156Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei463 – 4631PhosphothreonineBy similarity
Modified residuei466 – 4661PhosphotyrosineBy similarity
Modified residuei475 – 4751PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99JN2.
MaxQBiQ99JN2.
PaxDbiQ99JN2.
PRIDEiQ99JN2.

PTM databases

iPTMnetiQ99JN2.
PhosphoSiteiQ99JN2.

Expressioni

Gene expression databases

BgeeiQ99JN2.
CleanExiMM_KLHL22.
ExpressionAtlasiQ99JN2. baseline and differential.
GenevisibleiQ99JN2. MM.

Interactioni

Subunit structurei

Component of the BCR(KLHL22) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL22 and RBX1. Interacts with PLK1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi230228. 6 interactions.
IntActiQ99JN2. 6 interactions.
STRINGi10090.ENSMUSP00000112412.

Structurei

3D structure databases

ProteinModelPortaliQ99JN2.
SMRiQ99JN2. Positions 32-218, 264-590.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 11768BTBPROSITE-ProRule annotationAdd
BLAST
Repeati299 – 34951Kelch 1Add
BLAST
Repeati350 – 39950Kelch 2Add
BLAST
Repeati400 – 44647Kelch 3Add
BLAST
Repeati448 – 49346Kelch 4Add
BLAST
Repeati495 – 54450Kelch 5Add
BLAST
Repeati545 – 59349Kelch 6Add
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 6 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00760000118825.
HOGENOMiHOG000231583.
HOVERGENiHBG105890.
InParanoidiQ99JN2.
KOiK10459.
PhylomeDBiQ99JN2.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR030575. KLHL22.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PANTHERiPTHR24412:SF193. PTHR24412:SF193. 1 hit.
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 2 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99JN2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEEQDFAQL CRLPTQPSHS HCVNNTYRST QHSQALLRGL LALRDSGILF
60 70 80 90 100
DVVLVVEGKH IEAHRILLAA SCDYFRGMFA GGLKEMEQEE VLIHGVSYNA
110 120 130 140 150
MCQILHFIYT SELELSLSNV QETLVAACQL QIPEIIHFCC DFLMSWVDEE
160 170 180 190 200
NILDVYRLAD LFDLNHLTQQ LDTYILKNFV AFSRTDKYRQ LPLEKVYSLL
210 220 230 240 250
SSNRLEVSCE TEVYEGALLY HYSLEQVQAD QISLNEPPKL LETVRFPLME
260 270 280 290 300
AEVLQRLHDK LGPSPLRDTV ASALMYHRNE ILQPSLQGPQ TELRSDFQCV
310 320 330 340 350
VGFGGIHSTP STILSDQAKY LNPLLGEWKH FTASLAPRMS NQGIAVLNNF
360 370 380 390 400
VYLIGGDNNV QGFRAESRCW RYDPRHNRWF QIQSLQQEHA DLCVCVVGKY
410 420 430 440 450
IYAVAGRDYH NDLSAVERYD PATNSWDYVA PLKKEVYAHA GTTLQGKMYI
460 470 480 490 500
TCGRRGEDYL KETHCYDPGS NTWHTLADGP VRRAWHGMAA LLDKLFVIGG
510 520 530 540 550
SNNDAGYRRD VHQVACYSCT SRQWSSVCPL PAGHGEPGIA VLDSRIYVLG
560 570 580 590 600
GRSHNRGSRT GYVHIYDMEK DCWEEGPQLN NSISGLAACV LTLPRSLLHE
610 620 630
QPRGTPNRSQ ADADFASEVM SVSDWEEFDN SSED
Length:634
Mass (Da):71,687
Last modified:June 1, 2001 - v1
Checksum:iE34C21B5919620EB
GO
Isoform 2 (identifier: Q99JN2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     634-634: D → STPSHSQSLWLPFCEPWSVW

Show »
Length:653
Mass (Da):73,928
Checksum:iD65E9F4CCBF572AB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221C → L in BAC25806 (PubMed:16141072).Curated
Sequence conflicti428 – 4281Y → C in BAC25806 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei634 – 6341D → STPSHSQSLWLPFCEPWSVW in isoform 2. 1 PublicationVSP_019452

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028197 mRNA. Translation: BAC25806.1.
AK143514 mRNA. Translation: BAE25409.1.
AC087802 Genomic DNA. No translation available.
BC005800 mRNA. Translation: AAH05800.1.
CCDSiCCDS49783.1. [Q99JN2-1]
RefSeqiNP_663454.3. NM_145479.4. [Q99JN2-1]
XP_006522103.1. XM_006522040.2. [Q99JN2-1]
XP_006522104.1. XM_006522041.2. [Q99JN2-1]
XP_006536865.1. XM_006536802.2. [Q99JN2-1]
XP_006536866.1. XM_006536803.2. [Q99JN2-1]
UniGeneiMm.282807.

Genome annotation databases

EnsembliENSMUST00000120488; ENSMUSP00000112412; ENSMUSG00000022750. [Q99JN2-1]
ENSMUST00000165790; ENSMUSP00000127227; ENSMUSG00000022750. [Q99JN2-1]
GeneIDi224023.
KEGGimmu:224023.
UCSCiuc007ymc.2. mouse. [Q99JN2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028197 mRNA. Translation: BAC25806.1.
AK143514 mRNA. Translation: BAE25409.1.
AC087802 Genomic DNA. No translation available.
BC005800 mRNA. Translation: AAH05800.1.
CCDSiCCDS49783.1. [Q99JN2-1]
RefSeqiNP_663454.3. NM_145479.4. [Q99JN2-1]
XP_006522103.1. XM_006522040.2. [Q99JN2-1]
XP_006522104.1. XM_006522041.2. [Q99JN2-1]
XP_006536865.1. XM_006536802.2. [Q99JN2-1]
XP_006536866.1. XM_006536803.2. [Q99JN2-1]
UniGeneiMm.282807.

3D structure databases

ProteinModelPortaliQ99JN2.
SMRiQ99JN2. Positions 32-218, 264-590.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230228. 6 interactions.
IntActiQ99JN2. 6 interactions.
STRINGi10090.ENSMUSP00000112412.

PTM databases

iPTMnetiQ99JN2.
PhosphoSiteiQ99JN2.

Proteomic databases

EPDiQ99JN2.
MaxQBiQ99JN2.
PaxDbiQ99JN2.
PRIDEiQ99JN2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000120488; ENSMUSP00000112412; ENSMUSG00000022750. [Q99JN2-1]
ENSMUST00000165790; ENSMUSP00000127227; ENSMUSG00000022750. [Q99JN2-1]
GeneIDi224023.
KEGGimmu:224023.
UCSCiuc007ymc.2. mouse. [Q99JN2-1]

Organism-specific databases

CTDi84861.
MGIiMGI:1337995. Klhl22.

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00760000118825.
HOGENOMiHOG000231583.
HOVERGENiHBG105890.
InParanoidiQ99JN2.
KOiK10459.
PhylomeDBiQ99JN2.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ99JN2.
SOURCEiSearch...

Gene expression databases

BgeeiQ99JN2.
CleanExiMM_KLHL22.
ExpressionAtlasiQ99JN2. baseline and differential.
GenevisibleiQ99JN2. MM.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR030575. KLHL22.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PANTHERiPTHR24412:SF193. PTHR24412:SF193. 1 hit.
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 2 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. Bienvenut W.V., Sandilands E., Serrels B., Brunton V.G., Frame M.C.
    Submitted (FEB-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-12; 45-59; 66-76; 158-184; 196-204; 240-256; 268-294; 320-329; 419-434; 495-508 AND 596-603, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiKLH22_MOUSE
AccessioniPrimary (citable) accession number: Q99JN2
Secondary accession number(s): D3YW27, Q8BT13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.