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Protein

Kelch-like protein 22

Gene

Klhl22

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Kelch-like protein 22
Gene namesi
Name:Klhl22
Synonyms:Kelchl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1337995 Klhl22

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002421562 – 634Kelch-like protein 22Add BLAST633

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei463PhosphothreonineBy similarity1
Modified residuei466PhosphotyrosineBy similarity1
Modified residuei475PhosphothreonineBy similarity1
Modified residuei605PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99JN2
PaxDbiQ99JN2
PRIDEiQ99JN2

PTM databases

iPTMnetiQ99JN2
PhosphoSitePlusiQ99JN2

Expressioni

Gene expression databases

BgeeiENSMUSG00000022750
CleanExiMM_KLHL22
ExpressionAtlasiQ99JN2 baseline and differential
GenevisibleiQ99JN2 MM

Interactioni

Subunit structurei

Component of the BCR(KLHL22) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL22 and RBX1. Interacts with PLK1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi230228, 6 interactors
IntActiQ99JN2, 6 interactors
STRINGi10090.ENSMUSP00000112412

Structurei

3D structure databases

ProteinModelPortaliQ99JN2
SMRiQ99JN2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 117BTBPROSITE-ProRule annotationAdd BLAST68
Repeati299 – 349Kelch 1Add BLAST51
Repeati350 – 399Kelch 2Add BLAST50
Repeati400 – 446Kelch 3Add BLAST47
Repeati448 – 493Kelch 4Add BLAST46
Repeati495 – 544Kelch 5Add BLAST50
Repeati545 – 593Kelch 6Add BLAST49

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441 Eukaryota
ENOG410XNX8 LUCA
GeneTreeiENSGT00760000118825
HOGENOMiHOG000231583
HOVERGENiHBG105890
InParanoidiQ99JN2
KOiK10459
PhylomeDBiQ99JN2

Family and domain databases

Gene3Di2.120.10.80, 1 hit
InterProiView protein in InterPro
IPR011705 BACK
IPR017096 BTB-kelch_protein
IPR000210 BTB/POZ_dom
IPR015915 Kelch-typ_b-propeller
IPR006652 Kelch_1
IPR030575 KLHL22
IPR011333 SKP1/BTB/POZ_sf
PANTHERiPTHR24412:SF193 PTHR24412:SF193, 1 hit
PfamiView protein in Pfam
PF07707 BACK, 1 hit
PF00651 BTB, 1 hit
PF01344 Kelch_1, 2 hits
PIRSFiPIRSF037037 Kelch-like_protein_gigaxonin, 1 hit
SMARTiView protein in SMART
SM00875 BACK, 1 hit
SM00225 BTB, 1 hit
SM00612 Kelch, 6 hits
SUPFAMiSSF117281 SSF117281, 1 hit
SSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99JN2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEEQDFAQL CRLPTQPSHS HCVNNTYRST QHSQALLRGL LALRDSGILF
60 70 80 90 100
DVVLVVEGKH IEAHRILLAA SCDYFRGMFA GGLKEMEQEE VLIHGVSYNA
110 120 130 140 150
MCQILHFIYT SELELSLSNV QETLVAACQL QIPEIIHFCC DFLMSWVDEE
160 170 180 190 200
NILDVYRLAD LFDLNHLTQQ LDTYILKNFV AFSRTDKYRQ LPLEKVYSLL
210 220 230 240 250
SSNRLEVSCE TEVYEGALLY HYSLEQVQAD QISLNEPPKL LETVRFPLME
260 270 280 290 300
AEVLQRLHDK LGPSPLRDTV ASALMYHRNE ILQPSLQGPQ TELRSDFQCV
310 320 330 340 350
VGFGGIHSTP STILSDQAKY LNPLLGEWKH FTASLAPRMS NQGIAVLNNF
360 370 380 390 400
VYLIGGDNNV QGFRAESRCW RYDPRHNRWF QIQSLQQEHA DLCVCVVGKY
410 420 430 440 450
IYAVAGRDYH NDLSAVERYD PATNSWDYVA PLKKEVYAHA GTTLQGKMYI
460 470 480 490 500
TCGRRGEDYL KETHCYDPGS NTWHTLADGP VRRAWHGMAA LLDKLFVIGG
510 520 530 540 550
SNNDAGYRRD VHQVACYSCT SRQWSSVCPL PAGHGEPGIA VLDSRIYVLG
560 570 580 590 600
GRSHNRGSRT GYVHIYDMEK DCWEEGPQLN NSISGLAACV LTLPRSLLHE
610 620 630
QPRGTPNRSQ ADADFASEVM SVSDWEEFDN SSED
Length:634
Mass (Da):71,687
Last modified:June 1, 2001 - v1
Checksum:iE34C21B5919620EB
GO
Isoform 2 (identifier: Q99JN2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     634-634: D → STPSHSQSLWLPFCEPWSVW

Show »
Length:653
Mass (Da):73,928
Checksum:iD65E9F4CCBF572AB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22C → L in BAC25806 (PubMed:16141072).Curated1
Sequence conflicti428Y → C in BAC25806 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019452634D → STPSHSQSLWLPFCEPWSVW in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028197 mRNA Translation: BAC25806.1
AK143514 mRNA Translation: BAE25409.1
AC087802 Genomic DNA No translation available.
BC005800 mRNA Translation: AAH05800.1
CCDSiCCDS49783.1 [Q99JN2-1]
RefSeqiNP_663454.3, NM_145479.4 [Q99JN2-1]
XP_006522103.1, XM_006522040.3
XP_006522104.1, XM_006522041.3 [Q99JN2-1]
UniGeneiMm.282807

Genome annotation databases

EnsembliENSMUST00000120488; ENSMUSP00000112412; ENSMUSG00000022750 [Q99JN2-1]
ENSMUST00000165790; ENSMUSP00000127227; ENSMUSG00000022750 [Q99JN2-1]
GeneIDi224023
KEGGimmu:224023
UCSCiuc007ymc.2 mouse [Q99JN2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKLH22_MOUSE
AccessioniPrimary (citable) accession number: Q99JN2
Secondary accession number(s): D3YW27, Q8BT13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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