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Protein

Ras-related protein Rap-1b

Gene

Rap1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function (By similarity).By similarity

Enzyme regulationi

Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18GTPBy similarity9
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi116 – 119GTPBy similarity4
Nucleotide bindingi147 – 149GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: MGI
  • cellular response to cAMP Source: UniProtKB
  • cellular response to drug Source: Ensembl
  • cellular response to gonadotropin-releasing hormone Source: Ensembl
  • establishment of endothelial barrier Source: UniProtKB
  • negative regulation of calcium ion-dependent exocytosis Source: MGI
  • negative regulation of synaptic vesicle exocytosis Source: MGI
  • positive regulation of ERK1 and ERK2 cascade Source: MGI
  • Rap protein signal transduction Source: UniProtKB
  • regulation of cell junction assembly Source: UniProtKB
  • regulation of establishment of cell polarity Source: UniProtKB
  • response to carbohydrate Source: Ensembl
  • small GTPase mediated signal transduction Source: MGI

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-354192 Integrin alphaIIb beta3 signaling
R-MMU-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-MMU-372708 p130Cas linkage to MAPK signaling for integrins
R-MMU-392517 Rap1 signalling
R-MMU-5674135 MAP2K and MAPK activation
R-MMU-6798695 Neutrophil degranulation
R-MMU-8875555 MET activates RAP1 and RAC1

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-1b
Alternative name(s):
GTP-binding protein smg p21B
Gene namesi
Name:Rap1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:894315 Rap1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000302111 – 181Ras-related protein Rap-1bAdd BLAST181
PropeptideiPRO_0000030212182 – 184Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39ADP-ribosylserine; by botulinum toxinBy similarity1
Modified residuei179Phosphoserine; by PKABy similarity1
Modified residuei181Cysteine methyl esterBy similarity1
Lipidationi181S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

ADP-ribosylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ99JI6
MaxQBiQ99JI6
PaxDbiQ99JI6
PRIDEiQ99JI6
TopDownProteomicsiQ99JI6

PTM databases

iPTMnetiQ99JI6
PhosphoSitePlusiQ99JI6
SwissPalmiQ99JI6

Expressioni

Gene expression databases

BgeeiENSMUSG00000052681
CleanExiMM_RAP1B
ExpressionAtlasiQ99JI6 baseline and differential
GenevisibleiQ99JI6 MM

Interactioni

Subunit structurei

Interacts with SGSM1, SGSM2 and SGSM3. Interacts with KRIT1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi2296281 interactor.
IntActiQ99JI6 2 interactors.
MINTiQ99JI6
STRINGi10090.ENSMUSP00000066238

Structurei

3D structure databases

ProteinModelPortaliQ99JI6
SMRiQ99JI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 67Interaction with KRIT1By similarityAdd BLAST43

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133678
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiQ99JI6
KOiK07836
OMAiWGNAPYY
OrthoDBiEOG091G0OOG
PhylomeDBiQ99JI6
TreeFamiTF313014

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99JI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS
160 170 180
KINVNEIFYD LVRQINRKTP VPGKARKKSS CQLL
Length:184
Mass (Da):20,825
Last modified:May 10, 2004 - v2
Checksum:iCE976895E5965224
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56L → W in AAK14823 (PubMed:1555775).Curated1
Sequence conflicti61T → Q in AAK14823 (PubMed:1555775).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC033382 mRNA Translation: AAH33382.2
BC052480 mRNA Translation: AAH52480.1
M79314 mRNA Translation: AAK14823.1
CCDSiCCDS24196.1
PIRiJH0652
RefSeqiNP_077777.1, NM_024457.2
UniGeneiMm.233009
Mm.491159

Genome annotation databases

EnsembliENSMUST00000064667; ENSMUSP00000066238; ENSMUSG00000052681
GeneIDi215449
KEGGimmu:215449
UCSCiuc007hdr.1 mouse

Similar proteinsi

Entry informationi

Entry nameiRAP1B_MOUSE
AccessioniPrimary (citable) accession number: Q99JI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: April 25, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome