Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras-related protein Rap-1b

Gene

Rap1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function (By similarity).By similarity

Enzyme regulationi

Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi116 – 1194GTPBy similarity
Nucleotide bindingi147 – 1493GTPBy similarity

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: MGI
  • cellular response to cAMP Source: UniProtKB
  • cellular response to drug Source: Ensembl
  • cellular response to gonadotropin-releasing hormone Source: Ensembl
  • establishment of endothelial barrier Source: UniProtKB
  • negative regulation of calcium ion-dependent exocytosis Source: MGI
  • negative regulation of synaptic vesicle exocytosis Source: MGI
  • positive regulation of ERK1 and ERK2 cascade Source: MGI
  • Rap protein signal transduction Source: UniProtKB
  • regulation of cell junction assembly Source: UniProtKB
  • regulation of establishment of cell polarity Source: UniProtKB
  • response to carbohydrate Source: Ensembl
  • small GTPase mediated signal transduction Source: MGI
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-1b
Alternative name(s):
GTP-binding protein smg p21B
Gene namesi
Name:Rap1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:894315. Rap1b.

Subcellular locationi

  • Cell membrane By similarity
  • Cytoplasmcytosol By similarity
  • Cell junction By similarity

  • Note: May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Ras-related protein Rap-1bPRO_0000030211Add
BLAST
Propeptidei182 – 1843Removed in mature formBy similarityPRO_0000030212

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei39 – 391ADP-ribosylserine; by botulinum toxinBy similarity
Modified residuei179 – 1791Phosphoserine; by PKABy similarity
Modified residuei181 – 1811Cysteine methyl esterBy similarity
Lipidationi181 – 1811S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

ADP-ribosylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ99JI6.
MaxQBiQ99JI6.
PaxDbiQ99JI6.
PRIDEiQ99JI6.
TopDownProteomicsiQ99JI6.

PTM databases

iPTMnetiQ99JI6.
PhosphoSiteiQ99JI6.
SwissPalmiQ99JI6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000052681.
CleanExiMM_RAP1B.
ExpressionAtlasiQ99JI6. baseline and differential.
GenevisibleiQ99JI6. MM.

Interactioni

Subunit structurei

Interacts with SGSM1, SGSM2 and SGSM3. Interacts with KRIT1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229628. 1 interaction.
IntActiQ99JI6. 2 interactions.
MINTiMINT-4153353.
STRINGi10090.ENSMUSP00000066238.

Structurei

3D structure databases

ProteinModelPortaliQ99JI6.
SMRiQ99JI6. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 6743Interaction with KRIT1By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector regionCurated

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ99JI6.
KOiK07836.
OMAiRDSTHPR.
OrthoDBiEOG091G0OOG.
PhylomeDBiQ99JI6.
TreeFamiTF313014.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99JI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS
160 170 180
KINVNEIFYD LVRQINRKTP VPGKARKKSS CQLL
Length:184
Mass (Da):20,825
Last modified:May 10, 2004 - v2
Checksum:iCE976895E5965224
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561L → W in AAK14823 (PubMed:1555775).Curated
Sequence conflicti61 – 611T → Q in AAK14823 (PubMed:1555775).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC033382 mRNA. Translation: AAH33382.2.
BC052480 mRNA. Translation: AAH52480.1.
M79314 mRNA. Translation: AAK14823.1.
CCDSiCCDS24196.1.
PIRiJH0652.
RefSeqiNP_077777.1. NM_024457.2.
UniGeneiMm.233009.
Mm.491159.

Genome annotation databases

EnsembliENSMUST00000064667; ENSMUSP00000066238; ENSMUSG00000052681.
GeneIDi215449.
KEGGimmu:215449.
UCSCiuc007hdr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC033382 mRNA. Translation: AAH33382.2.
BC052480 mRNA. Translation: AAH52480.1.
M79314 mRNA. Translation: AAK14823.1.
CCDSiCCDS24196.1.
PIRiJH0652.
RefSeqiNP_077777.1. NM_024457.2.
UniGeneiMm.233009.
Mm.491159.

3D structure databases

ProteinModelPortaliQ99JI6.
SMRiQ99JI6. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229628. 1 interaction.
IntActiQ99JI6. 2 interactions.
MINTiMINT-4153353.
STRINGi10090.ENSMUSP00000066238.

PTM databases

iPTMnetiQ99JI6.
PhosphoSiteiQ99JI6.
SwissPalmiQ99JI6.

Proteomic databases

EPDiQ99JI6.
MaxQBiQ99JI6.
PaxDbiQ99JI6.
PRIDEiQ99JI6.
TopDownProteomicsiQ99JI6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064667; ENSMUSP00000066238; ENSMUSG00000052681.
GeneIDi215449.
KEGGimmu:215449.
UCSCiuc007hdr.1. mouse.

Organism-specific databases

CTDi5908.
MGIiMGI:894315. Rap1b.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ99JI6.
KOiK07836.
OMAiRDSTHPR.
OrthoDBiEOG091G0OOG.
PhylomeDBiQ99JI6.
TreeFamiTF313014.

Miscellaneous databases

ChiTaRSiRap1b. mouse.
PROiQ99JI6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052681.
CleanExiMM_RAP1B.
ExpressionAtlasiQ99JI6. baseline and differential.
GenevisibleiQ99JI6. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAP1B_MOUSE
AccessioniPrimary (citable) accession number: Q99JI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: September 7, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.