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Protein

PC4 and SFRS1-interacting protein

Gene

Psip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
PC4 and SFRS1-interacting protein
Alternative name(s):
Lens epithelium-derived growth factor
Short name:
mLEDGF
Gene namesi
Name:Psip1
Synonyms:Ledgf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2142116. Psip1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001917091 – 528PC4 and SFRS1-interacting proteinAdd BLAST528

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102PhosphoserineBy similarity1
Modified residuei105PhosphoserineCombined sources1
Modified residuei106PhosphoserineCombined sources1
Modified residuei115PhosphothreonineCombined sources1
Modified residuei122PhosphothreonineCombined sources1
Modified residuei129PhosphoserineCombined sources1
Modified residuei141PhosphothreonineCombined sources1
Modified residuei176PhosphoserineCombined sources1
Modified residuei205PhosphoserineBy similarity1
Modified residuei270PhosphoserineCombined sources1
Modified residuei271PhosphothreonineCombined sources1
Modified residuei272PhosphoserineCombined sources1
Modified residuei274PhosphoserineCombined sources1
Modified residuei432PhosphoserineBy similarity1
Modified residuei435PhosphothreonineBy similarity1
Modified residuei441PhosphoserineBy similarity1
Modified residuei515Citrulline1 Publication1
Modified residuei520PhosphoserineCombined sources1
Modified residuei525PhosphothreonineBy similarity1

Post-translational modificationi

Citrullinated by PADI4.1 Publication

Keywords - PTMi

Citrullination, Phosphoprotein

Proteomic databases

EPDiQ99JF8.
PaxDbiQ99JF8.
PeptideAtlasiQ99JF8.
PRIDEiQ99JF8.

PTM databases

iPTMnetiQ99JF8.
PhosphoSitePlusiQ99JF8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028484.
CleanExiMM_PSIP1.
ExpressionAtlasiQ99JF8. baseline and differential.
GenevisibleiQ99JF8. MM.

Interactioni

Subunit structurei

Interacts with IFRD1/PC4. Interacts POGZ and CDCA7L (By similarity). Murine leukemia virus (MLV) integrase does not interact with PSIP1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221716. 3 interactors.
IntActiQ99JF8. 2 interactors.
MINTiMINT-4123368.
STRINGi10090.ENSMUSP00000030207.

Structurei

3D structure databases

ProteinModelPortaliQ99JF8.
SMRiQ99JF8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 64PWWPPROSITE-ProRule annotationAdd BLAST58

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili306 – 332Sequence analysisAdd BLAST27
Coiled coili369 – 393Sequence analysisAdd BLAST25

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi146 – 156Nuclear localization signalBy similarityAdd BLAST11

Sequence similaritiesi

Belongs to the HDGF family.Curated
Contains 1 PWWP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1904. Eukaryota.
ENOG410Y5WD. LUCA.
GeneTreeiENSGT00530000063013.
HOVERGENiHBG108300.
InParanoidiQ99JF8.
OMAiESKDSHE.
OrthoDBiEOG091G045X.
PhylomeDBiQ99JF8.
TreeFamiTF105385.

Family and domain databases

InterProiIPR021567. LEDGF.
IPR000313. PWWP_dom.
IPR017859. Treacle-like_TCS.
[Graphical view]
PfamiPF11467. LEDGF. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
PRINTSiPR01503. TREACLE.
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99JF8-1) [UniParc]FASTAAdd to basket
Also known as: Ledgfa, p75

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTRDFKPGDL IFAKMKGYPH WPARVDEVPD GAVKPPTNKL PIFFFGTHET
60 70 80 90 100
AFLGPKDIFP YSENKEKYGK PNKRKGFNEG LWEIDNNPKV KFSSQQASTK
110 120 130 140 150
QSNASSDVEV EEKETNVSKE DTDQEEKASN EDVTKAVDIT TPKAARRGRK
160 170 180 190 200
RKAEKQVDTE EAGMVTAATA SNVKASPKRG RPAATEVKIP KPRGRPKVVK
210 220 230 240 250
QPCPSDGDMV IDEDKSKKKG PEEKQPKKQL KKEEEGQKEE EKPRKEPDKK
260 270 280 290 300
EGKKEVESKR KNLAKPGVTS TSDSEDEDDQ EGEKKRKGGR NFQAAHRRNM
310 320 330 340 350
LKGQHEKEAG DRKRKQEEQM ETEQQNKDEG KKPEVKKVEK KRETSMDSRL
360 370 380 390 400
QRIHAEIKNS LKIDNLDVNR CIEALDELAS LQVTMQQAQK HTEMITTLKK
410 420 430 440 450
IRRFKVSQVI MEKSTMLYNK FKNMFLVGEG DSVITQVLNK SLAEQRQHEE
460 470 480 490 500
ANKTKDQGKK GPNKKLEKEP TGTKSLNGGS DAQESNHPQH NGDSNEDGKD
510 520
SREASSKTKP PGEEREAEIS LKESTLDN
Length:528
Mass (Da):59,697
Last modified:June 1, 2001 - v1
Checksum:i4A9AE28245843AB6
GO
Isoform 2 (identifier: Q99JF8-2) [UniParc]FASTAAdd to basket
Also known as: Ledgfb, p52

The sequence of this isoform differs from the canonical sequence as follows:
     324-331: QQNKDEGK → HQTTCNLQ
     332-528: Missing.

Show »
Length:331
Mass (Da):37,426
Checksum:i516F01D99A5DD5A6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform 2 (identifier: Q99JF8-2)
Sequence conflicti324 – 331HQTTCNLQ → QLKALIQ in AAH52177 (PubMed:15489334).Curated8

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014299324 – 331QQNKDEGK → HQTTCNLQ in isoform 2. 3 Publications8
Alternative sequenceiVSP_014300332 – 528Missing in isoform 2. 3 PublicationsAdd BLAST197

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ308965 mRNA. Translation: CAC34944.1.
AJ308966 mRNA. Translation: CAC34945.1.
AF339082 mRNA. Translation: AAO32949.1.
AF339083 mRNA. Translation: AAO32950.1.
BX682545 Genomic DNA. Translation: CAM20466.1.
BX682545 Genomic DNA. Translation: CAM20467.1.
BC002260 mRNA. Translation: AAH02260.1.
BC043079 mRNA. Translation: AAH43079.1.
BC052177 mRNA. Translation: AAH52177.1.
AK011572 mRNA. Translation: BAB27707.3.
AK139598 mRNA. Translation: BAE24079.1.
AK161426 mRNA. Translation: BAE36388.1.
AK169600 mRNA. Translation: BAE41251.1.
CCDSiCCDS18299.1. [Q99JF8-1]
CCDS71412.1. [Q99JF8-2]
RefSeqiNP_001277456.1. NM_001290527.1. [Q99JF8-2]
NP_598709.1. NM_133948.5. [Q99JF8-1]
UniGeneiMm.105331.
Mm.260943.
Mm.448301.
Mm.451848.

Genome annotation databases

EnsembliENSMUST00000030207; ENSMUSP00000030207; ENSMUSG00000028484. [Q99JF8-1]
ENSMUST00000107215; ENSMUSP00000102833; ENSMUSG00000028484. [Q99JF8-2]
GeneIDi101739.
KEGGimmu:101739.
UCSCiuc008tkw.2. mouse. [Q99JF8-1]
uc008tkx.2. mouse. [Q99JF8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ308965 mRNA. Translation: CAC34944.1.
AJ308966 mRNA. Translation: CAC34945.1.
AF339082 mRNA. Translation: AAO32949.1.
AF339083 mRNA. Translation: AAO32950.1.
BX682545 Genomic DNA. Translation: CAM20466.1.
BX682545 Genomic DNA. Translation: CAM20467.1.
BC002260 mRNA. Translation: AAH02260.1.
BC043079 mRNA. Translation: AAH43079.1.
BC052177 mRNA. Translation: AAH52177.1.
AK011572 mRNA. Translation: BAB27707.3.
AK139598 mRNA. Translation: BAE24079.1.
AK161426 mRNA. Translation: BAE36388.1.
AK169600 mRNA. Translation: BAE41251.1.
CCDSiCCDS18299.1. [Q99JF8-1]
CCDS71412.1. [Q99JF8-2]
RefSeqiNP_001277456.1. NM_001290527.1. [Q99JF8-2]
NP_598709.1. NM_133948.5. [Q99JF8-1]
UniGeneiMm.105331.
Mm.260943.
Mm.448301.
Mm.451848.

3D structure databases

ProteinModelPortaliQ99JF8.
SMRiQ99JF8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221716. 3 interactors.
IntActiQ99JF8. 2 interactors.
MINTiMINT-4123368.
STRINGi10090.ENSMUSP00000030207.

PTM databases

iPTMnetiQ99JF8.
PhosphoSitePlusiQ99JF8.

Proteomic databases

EPDiQ99JF8.
PaxDbiQ99JF8.
PeptideAtlasiQ99JF8.
PRIDEiQ99JF8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030207; ENSMUSP00000030207; ENSMUSG00000028484. [Q99JF8-1]
ENSMUST00000107215; ENSMUSP00000102833; ENSMUSG00000028484. [Q99JF8-2]
GeneIDi101739.
KEGGimmu:101739.
UCSCiuc008tkw.2. mouse. [Q99JF8-1]
uc008tkx.2. mouse. [Q99JF8-2]

Organism-specific databases

CTDi11168.
MGIiMGI:2142116. Psip1.

Phylogenomic databases

eggNOGiKOG1904. Eukaryota.
ENOG410Y5WD. LUCA.
GeneTreeiENSGT00530000063013.
HOVERGENiHBG108300.
InParanoidiQ99JF8.
OMAiESKDSHE.
OrthoDBiEOG091G045X.
PhylomeDBiQ99JF8.
TreeFamiTF105385.

Miscellaneous databases

ChiTaRSiPsip1. mouse.
PROiQ99JF8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028484.
CleanExiMM_PSIP1.
ExpressionAtlasiQ99JF8. baseline and differential.
GenevisibleiQ99JF8. MM.

Family and domain databases

InterProiIPR021567. LEDGF.
IPR000313. PWWP_dom.
IPR017859. Treacle-like_TCS.
[Graphical view]
PfamiPF11467. LEDGF. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
PRINTSiPR01503. TREACLE.
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSIP1_MOUSE
AccessioniPrimary (citable) accession number: Q99JF8
Secondary accession number(s): A2BI10
, A2BI11, Q3TEJ7, Q3TTD7, Q3UTA1, Q80WQ7, Q99JF7, Q99LR4, Q9CT03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.