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Q99JE6

- PLCB3_RAT

UniProt

Q99JE6 - PLCB3_RAT

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

Gene

Plcb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.By similarity1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Calcium.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei330 – 3301PROSITE-ProRule annotation
Active sitei377 – 3771PROSITE-ProRule annotation

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB
  3. phospholipase C activity Source: RGD
  4. signal transducer activity Source: UniProtKB

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: RGD
  2. intracellular signal transduction Source: UniProtKB
  3. phospholipid catabolic process Source: UniProtKB
  4. post-Golgi vesicle-mediated transport Source: RGD
  5. synaptic transmission Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BRENDAi3.1.4.11. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-beta-3
Phospholipase C-beta-3
Short name:
PLC-beta-3
Gene namesi
Name:Plcb3
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi61993. Plcb3.

Subcellular locationi

Cytoplasm 1 Publication. Membrane 1 Publication
Note: And particulate fractions.

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. sarcolemma Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 123412331-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3PRO_0000088493Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei472 – 4721PhosphoserineBy similarity
Modified residuei535 – 5351PhosphoserineBy similarity
Modified residuei1105 – 11051PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ99JE6.
PRIDEiQ99JE6.

PTM databases

PhosphoSiteiQ99JE6.

Expressioni

Tissue specificityi

Expressed in parotid gland, brain, liver, uterus, lung, heart, adrenal gland, and ovary. Not detected in spleen, pancreas, intestine, thymus or kidney.1 Publication

Gene expression databases

GenevestigatoriQ99JE6.

Interactioni

Subunit structurei

Interacts with LPAR2 By similarity. Interacts with SHANK2.By similarity1 Publication

Protein-protein interaction databases

MINTiMINT-268643.

Structurei

3D structure databases

ProteinModelPortaliQ99JE6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini315 – 466152PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini589 – 705117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini712 – 80998C2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1231 – 12344Interaction with SHANK2

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
HOVERGENiHBG053609.
InParanoidiQ99JE6.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028390. PLC-beta3.
IPR014815. PLC-beta_C.
IPR009535. PLC-beta_CS.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF11. PTHR10336:SF11. 1 hit.
PfamiPF00168. C2. 1 hit.
PF06631. DUF1154. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99JE6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGARPGVHA LQLEPPTVVE TLRRGSKFIK WDEEASSRNL VTLRLDPNGF
60 70 80 90 100
FLYWTGPNME VDTLDISSIR DTRTGRYARL PKDPKIREVL GFGGPDTRLE
110 120 130 140 150
EKLMTVVAGP DPVNTTFLNF MAVQDDTVKV WSEELFKLAM NILAQNAPEH
160 170 180 190 200
VLRKAYTKLK LQVNQDGRIP VKNILKMFSA DKKRVETALI CGLNFNRSES
210 220 230 240 250
IRPDEFSLEI FERFLNKLLL RPDIDKILLE IGAKGKPYLT LEQLMDFINQ
260 270 280 290 300
KQRDPRLNEV LYPPLRSSQA RLLIEKYEPN KQFLERDQMS MEGFSRYLGG
310 320 330 340 350
EENGILPLEA LDLSMDMTQP LSAYFINSSH NTYLTAGQLA GTSSVEMYRQ
360 370 380 390 400
ALLWGCRCVE LDVWKGRPPE EEPFITHGFT MTTEVPLRDV LEAIAETAFK
410 420 430 440 450
TSPYPVILSF ENHVDSAKQQ AKMAEYCRSI FGEALLIDPL DKYPLSAGTP
460 470 480 490 500
LPSPQDLMGR ILVKNKKRHR PSTGVPDSSV AKRPLEQSNS ALSESSAATE
510 520 530 540 550
PSSPQLGSPS SDSCPGLSNG EEVGLEKTSL EPQKSLGEEG LNRGPNVLMP
560 570 580 590 600
DRDREDEEED EEEEETTDPK KPTTDEGTAS SEVNATEEMS TLVNYVEPVK
610 620 630 640 650
FKSFEASRKR NKCFEMSSFV ETKAMEQLTK SPMEFVEYNK QQLSRIYPKG
660 670 680 690 700
TRVDSSNYMP QLFWNVGCQL VALNFQTLDL PMQLNAGVFE YNGRSGYLLK
710 720 730 740 750
PEFMRRPDKS FDPFTEVIVD GIVANALRVK VISGQFLSDR KVGIYVEVDM
760 770 780 790 800
FGLPVDTRRK YRTRTSQGNS FNPVWDEEPF DFPKVVLPTL ASLRIAAFEE
810 820 830 840 850
GGRFVGHRIL PVSAIRSGYH YVCLRNEANQ PLCLPALLIY TEASDYIPDD
860 870 880 890 900
HQDYAEALIN PIKHVSLMDQ RAKQLAALIG ESEAQASTEM CQETPSQQQG
910 920 930 940 950
SQLSSNPVPN PLDDSPRWPP GPTTSPTSTS LSSPGQRDDL IASILSEVTP
960 970 980 990 1000
TPLEELRSHK AMVKLRSRQD RDLRELHKKH QRKAVALTRR LLDGLAQARA
1010 1020 1030 1040 1050
EGKCRPSSSA LSRATNVEDV KEEEKEAARQ YREFQNRQVQ SLLELREAQA
1060 1070 1080 1090 1100
DAETERRLEH LKQAQQRLRE VVLDAHTTQF KRLKELNERE KKELQKILDR
1110 1120 1130 1140 1150
KRNNSISEAK TREKHKKEVE LTEINRRHIT ESVNSIRRLE EAQKQRHERL
1160 1170 1180 1190 1200
LAGQQQVLQQ LVEEEPKLVA QLTQECQEQR ERLPQEIRRC LLGETSEGLG
1210 1220 1230
DGPLVACASN GHAAGSGGHQ SGADSESQEE NTQL
Length:1,234
Mass (Da):139,450
Last modified:October 1, 2002 - v2
Checksum:i2EBD7282F052CA6A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171T → A in AAK14906. (PubMed:8454637)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U41411 Genomic DNA. Translation: AAC53366.1.
M99567 mRNA. Translation: AAK14906.1.
PIRiA45493.
UniGeneiRn.16983.

Genome annotation databases

UCSCiRGD:61993. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U41411 Genomic DNA. Translation: AAC53366.1 .
M99567 mRNA. Translation: AAK14906.1 .
PIRi A45493.
UniGenei Rn.16983.

3D structure databases

ProteinModelPortali Q99JE6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-268643.

PTM databases

PhosphoSitei Q99JE6.

Proteomic databases

PaxDbi Q99JE6.
PRIDEi Q99JE6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:61993. rat.

Organism-specific databases

RGDi 61993. Plcb3.

Phylogenomic databases

eggNOGi NOG149692.
HOVERGENi HBG053609.
InParanoidi Q99JE6.

Enzyme and pathway databases

BRENDAi 3.1.4.11. 5301.

Miscellaneous databases

PROi Q99JE6.

Gene expression databases

Genevestigatori Q99JE6.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028390. PLC-beta3.
IPR014815. PLC-beta_C.
IPR009535. PLC-beta_CS.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF11. PTHR10336:SF11. 1 hit.
Pfami PF00168. C2. 1 hit.
PF06631. DUF1154. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000956. PLC-beta. 1 hit.
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The 5'-upstream region of the rat phospholipase C-beta 3 gene contains two critical Sp1 sites and an HIV Inr-like element."
    Kang J.S., Lee H.B., Rhee S.G., Park K., Yoo O.J.
    Gene 197:19-28(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33.
  2. "Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3."
    Jhon D.-Y., Lee H.-H., Park D., Lee C.-W., Lee K.-H., Yoo O.J., Rhee S.G.
    J. Biol. Chem. 268:6654-6661(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-1234, FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: Fischer.
    Tissue: Thyroid.
  3. "The interaction of phospholipase C-beta3 with Shank2 regulates mGluR-mediated calcium signal."
    Hwang J.-I., Kim H.S., Lee J.R., Kim E., Ryu S.H., Suh P.-G.
    J. Biol. Chem. 280:12467-12473(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SHANK2.

Entry informationi

Entry nameiPLCB3_RAT
AccessioniPrimary (citable) accession number: Q99JE6
Secondary accession number(s): Q62887, Q9QW29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3