1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

Preferred order of sections

Names and origin

Protein names

Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3
EC=3.1.4.11

Alternative name(s):
Phosphoinositide phospholipase C-beta-3
Phospholipase C-beta-3
Short name=PLC-beta-3

Gene names

Name:

Plcb3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1234 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Ref.2 UniProtKB P51432
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H₂O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. Ref.2
Cofactor	Calcium. Ref.2
Subunit structure	Interacts with LPAR2 By similarity. Interacts with SHANK2. Ref.3
Subcellular location	Cytoplasm. Membrane. Note: And particulate fractions. Ref.2
Tissue specificity	Expressed in parotid gland, brain, liver, uterus, lung, heart, adrenal gland, and ovary. Not detected in spleen, pancreas, intestine, thymus or kidney. Ref.2
Sequence similarities	Contains 1 C2 domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Cytoplasm Membrane
Ligand	Calcium
Molecular function	Hydrolase Transducer
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Inferred from mutant phenotype PubMed 16314422. Source: RGD intracellular signal transduction Non-traceable author statement Ref.2. Source: UniProtKB phospholipid catabolic process Inferred from direct assay Ref.2. Source: UniProtKB post-Golgi vesicle-mediated transport Inferred from direct assay PubMed 17492941. Source: RGD
Cellular_component	cytosol Inferred from direct assay Ref.2. Source: UniProtKB sarcolemma Inferred from direct assay PubMed 15362504. Source: RGD
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro phosphatidylinositol phospholipase C activity Inferred from direct assay Ref.2. Source: UniProtKB signal transducer activity Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1234

1234

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

PRO_0000088493

Regions

Domain

315 – 466

152

PI-PLC X-box

Domain

589 – 705

117

PI-PLC Y-box

Domain

712 – 809

98

C2

Region

1231 – 1234

4

Interaction with SHANK2

Sites

Active site

330

1

By similarity

Active site

377

1

By similarity UniProtKB P10688

Amino acid modifications

Modified residue

472

1

Phosphoserine By similarity

Modified residue

535

1

Phosphoserine By similarity

Modified residue

1105

1

Phosphoserine By similarity

Experimental info

Sequence conflict

17

1

T → A in AAK14906. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q99JE6 [UniParc].

Last modified October 1, 2002. Version 2.
Checksum: 2EBD7282F052CA6A
Show »

FASTA

1,234

139,450

        10         20         30         40         50         60 
MAGARPGVHA LQLEPPTVVE TLRRGSKFIK WDEEASSRNL VTLRLDPNGF FLYWTGPNME 

        70         80         90        100        110        120 
VDTLDISSIR DTRTGRYARL PKDPKIREVL GFGGPDTRLE EKLMTVVAGP DPVNTTFLNF 

       130        140        150        160        170        180 
MAVQDDTVKV WSEELFKLAM NILAQNAPEH VLRKAYTKLK LQVNQDGRIP VKNILKMFSA 

       190        200        210        220        230        240 
DKKRVETALI CGLNFNRSES IRPDEFSLEI FERFLNKLLL RPDIDKILLE IGAKGKPYLT 

       250        260        270        280        290        300 
LEQLMDFINQ KQRDPRLNEV LYPPLRSSQA RLLIEKYEPN KQFLERDQMS MEGFSRYLGG 

       310        320        330        340        350        360 
EENGILPLEA LDLSMDMTQP LSAYFINSSH NTYLTAGQLA GTSSVEMYRQ ALLWGCRCVE 

       370        380        390        400        410        420 
LDVWKGRPPE EEPFITHGFT MTTEVPLRDV LEAIAETAFK TSPYPVILSF ENHVDSAKQQ 

       430        440        450        460        470        480 
AKMAEYCRSI FGEALLIDPL DKYPLSAGTP LPSPQDLMGR ILVKNKKRHR PSTGVPDSSV 

       490        500        510        520        530        540 
AKRPLEQSNS ALSESSAATE PSSPQLGSPS SDSCPGLSNG EEVGLEKTSL EPQKSLGEEG 

       550        560        570        580        590        600 
LNRGPNVLMP DRDREDEEED EEEEETTDPK KPTTDEGTAS SEVNATEEMS TLVNYVEPVK 

       610        620        630        640        650        660 
FKSFEASRKR NKCFEMSSFV ETKAMEQLTK SPMEFVEYNK QQLSRIYPKG TRVDSSNYMP 

       670        680        690        700        710        720 
QLFWNVGCQL VALNFQTLDL PMQLNAGVFE YNGRSGYLLK PEFMRRPDKS FDPFTEVIVD 

       730        740        750        760        770        780 
GIVANALRVK VISGQFLSDR KVGIYVEVDM FGLPVDTRRK YRTRTSQGNS FNPVWDEEPF 

       790        800        810        820        830        840 
DFPKVVLPTL ASLRIAAFEE GGRFVGHRIL PVSAIRSGYH YVCLRNEANQ PLCLPALLIY 

       850        860        870        880        890        900 
TEASDYIPDD HQDYAEALIN PIKHVSLMDQ RAKQLAALIG ESEAQASTEM CQETPSQQQG 

       910        920        930        940        950        960 
SQLSSNPVPN PLDDSPRWPP GPTTSPTSTS LSSPGQRDDL IASILSEVTP TPLEELRSHK 

       970        980        990       1000       1010       1020 
AMVKLRSRQD RDLRELHKKH QRKAVALTRR LLDGLAQARA EGKCRPSSSA LSRATNVEDV 

      1030       1040       1050       1060       1070       1080 
KEEEKEAARQ YREFQNRQVQ SLLELREAQA DAETERRLEH LKQAQQRLRE VVLDAHTTQF 

      1090       1100       1110       1120       1130       1140 
KRLKELNERE KKELQKILDR KRNNSISEAK TREKHKKEVE LTEINRRHIT ESVNSIRRLE 

      1150       1160       1170       1180       1190       1200 
EAQKQRHERL LAGQQQVLQQ LVEEEPKLVA QLTQECQEQR ERLPQEIRRC LLGETSEGLG 

      1210       1220       1230 
DGPLVACASN GHAAGSGGHQ SGADSESQEE NTQL

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References

[1]	"The 5'-upstream region of the rat phospholipase C-beta 3 gene contains two critical Sp1 sites and an HIV Inr-like element." Kang J.S., Lee H.B., Rhee S.G., Park K., Yoo O.J. Gene 197:19-28(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33.
[2]	"Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3." Jhon D.-Y., Lee H.-H., Park D., Lee C.-W., Lee K.-H., Yoo O.J., Rhee S.G. J. Biol. Chem. 268:6654-6661(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-1234, FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Strain: Fischer. Tissue: Thyroid.
[3]	"The interaction of phospholipase C-beta3 with Shank2 regulates mGluR-mediated calcium signal." Hwang J.-I., Kim H.S., Lee J.R., Kim E., Ryu S.H., Suh P.-G. J. Biol. Chem. 280:12467-12473(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SHANK2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41411 Genomic DNA. Translation: AAC53366.1. M99567 mRNA. Translation: AAK14906.1.
IPI	IPI00209033.
PIR	A45493.
UniGene	Rn.16983.
3D structure databases
ProteinModelPortal	Q99JE6.
ModBase	Search...
PTM databases
PhosphoSite	Q99JE6.
Proteomic databases
PaxDb	Q99JE6.
PRIDE	Q99JE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:61993. rat.
Organism-specific databases
RGD	61993. Plcb3.
Phylogenomic databases
eggNOG	NOG149692.
HOVERGEN	HBG053609.
InParanoid	Q99JE6.
OrthoDB	EOG41RPT7.
Enzyme and pathway databases
BRENDA	3.1.4.11. 5301.
Gene expression databases
ArrayExpress	Q99JE6.
Genevestigator	Q99JE6.
GermOnline	ENSRNOG00000021150. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit. 3.20.20.190. 2 hits.
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011992. EF-hand-like_dom. IPR001192. Pinositol_PLipase_C. IPR016280. PLC-beta. IPR014815. PLC-beta_C. IPR009535. PLC-beta_CS. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR015359. PLipase_C_EF-hand-like. IPR000909. PLipase_C_PInositol-sp_X_dom. IPR001711. PLipase_C_Pinositol-sp_Y. [Graphical view]
PANTHER	PTHR10336. PTHR10336. 1 hit.
Pfam	PF00168. C2. 1 hit. PF06631. DUF1154. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. PF08703. PLC-beta_C. 1 hit. [Graphical view]
PIRSF	PIRSF000956. PLC-beta. 1 hit.
PRINTS	PR00390. PHPHLIPASEC.
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF51695. PLC-like_Pdiesterase_TIM-brl. 1 hit.
PROSITE	PS50004. C2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLCB3_RAT

Accession

Primary (citable) accession number: Q99JE6
Secondary accession number(s): Q62887, Q9QW29

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	October 1, 2002
Last modified:	April 3, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents