Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q99JE6 (PLCB3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C-beta-3
Phospholipase C-beta-3
Short name=PLC-beta-3
Gene names
Name:Plcb3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1234 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Ref.2 UniProtKB P51432

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. Ref.2

Cofactor

Calcium. Ref.2

Subunit structure

Interacts with LPAR2 By similarity. Interacts with SHANK2. Ref.3

Subcellular location

Cytoplasm. Membrane. Note: And particulate fractions. Ref.2

Tissue specificity

Expressed in parotid gland, brain, liver, uterus, lung, heart, adrenal gland, and ovary. Not detected in spleen, pancreas, intestine, thymus or kidney. Ref.2

Sequence similarities

Contains 1 C2 domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 123412331-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3
PRO_0000088493

Regions

Domain315 – 466152PI-PLC X-box
Domain589 – 705117PI-PLC Y-box
Domain712 – 80998C2
Region1231 – 12344Interaction with SHANK2

Sites

Active site3301 By similarity
Active site3771 By similarity UniProtKB P10688

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue4721Phosphoserine By similarity
Modified residue5351Phosphoserine By similarity
Modified residue11051Phosphoserine By similarity

Experimental info

Sequence conflict171T → A in AAK14906. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q99JE6 [UniParc].

Last modified October 1, 2002. Version 2.
Checksum: 2EBD7282F052CA6A

FASTA1,234139,450
        10         20         30         40         50         60 
MAGARPGVHA LQLEPPTVVE TLRRGSKFIK WDEEASSRNL VTLRLDPNGF FLYWTGPNME 

        70         80         90        100        110        120 
VDTLDISSIR DTRTGRYARL PKDPKIREVL GFGGPDTRLE EKLMTVVAGP DPVNTTFLNF 

       130        140        150        160        170        180 
MAVQDDTVKV WSEELFKLAM NILAQNAPEH VLRKAYTKLK LQVNQDGRIP VKNILKMFSA 

       190        200        210        220        230        240 
DKKRVETALI CGLNFNRSES IRPDEFSLEI FERFLNKLLL RPDIDKILLE IGAKGKPYLT 

       250        260        270        280        290        300 
LEQLMDFINQ KQRDPRLNEV LYPPLRSSQA RLLIEKYEPN KQFLERDQMS MEGFSRYLGG 

       310        320        330        340        350        360 
EENGILPLEA LDLSMDMTQP LSAYFINSSH NTYLTAGQLA GTSSVEMYRQ ALLWGCRCVE 

       370        380        390        400        410        420 
LDVWKGRPPE EEPFITHGFT MTTEVPLRDV LEAIAETAFK TSPYPVILSF ENHVDSAKQQ 

       430        440        450        460        470        480 
AKMAEYCRSI FGEALLIDPL DKYPLSAGTP LPSPQDLMGR ILVKNKKRHR PSTGVPDSSV 

       490        500        510        520        530        540 
AKRPLEQSNS ALSESSAATE PSSPQLGSPS SDSCPGLSNG EEVGLEKTSL EPQKSLGEEG 

       550        560        570        580        590        600 
LNRGPNVLMP DRDREDEEED EEEEETTDPK KPTTDEGTAS SEVNATEEMS TLVNYVEPVK 

       610        620        630        640        650        660 
FKSFEASRKR NKCFEMSSFV ETKAMEQLTK SPMEFVEYNK QQLSRIYPKG TRVDSSNYMP 

       670        680        690        700        710        720 
QLFWNVGCQL VALNFQTLDL PMQLNAGVFE YNGRSGYLLK PEFMRRPDKS FDPFTEVIVD 

       730        740        750        760        770        780 
GIVANALRVK VISGQFLSDR KVGIYVEVDM FGLPVDTRRK YRTRTSQGNS FNPVWDEEPF 

       790        800        810        820        830        840 
DFPKVVLPTL ASLRIAAFEE GGRFVGHRIL PVSAIRSGYH YVCLRNEANQ PLCLPALLIY 

       850        860        870        880        890        900 
TEASDYIPDD HQDYAEALIN PIKHVSLMDQ RAKQLAALIG ESEAQASTEM CQETPSQQQG 

       910        920        930        940        950        960 
SQLSSNPVPN PLDDSPRWPP GPTTSPTSTS LSSPGQRDDL IASILSEVTP TPLEELRSHK 

       970        980        990       1000       1010       1020 
AMVKLRSRQD RDLRELHKKH QRKAVALTRR LLDGLAQARA EGKCRPSSSA LSRATNVEDV 

      1030       1040       1050       1060       1070       1080 
KEEEKEAARQ YREFQNRQVQ SLLELREAQA DAETERRLEH LKQAQQRLRE VVLDAHTTQF 

      1090       1100       1110       1120       1130       1140 
KRLKELNERE KKELQKILDR KRNNSISEAK TREKHKKEVE LTEINRRHIT ESVNSIRRLE 

      1150       1160       1170       1180       1190       1200 
EAQKQRHERL LAGQQQVLQQ LVEEEPKLVA QLTQECQEQR ERLPQEIRRC LLGETSEGLG 

      1210       1220       1230 
DGPLVACASN GHAAGSGGHQ SGADSESQEE NTQL 

« Hide

References

[1]"The 5'-upstream region of the rat phospholipase C-beta 3 gene contains two critical Sp1 sites and an HIV Inr-like element."
Kang J.S., Lee H.B., Rhee S.G., Park K., Yoo O.J.
Gene 197:19-28(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33.
[2]"Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3."
Jhon D.-Y., Lee H.-H., Park D., Lee C.-W., Lee K.-H., Yoo O.J., Rhee S.G.
J. Biol. Chem. 268:6654-6661(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-1234, FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Strain: Fischer.
Tissue: Thyroid.
[3]"The interaction of phospholipase C-beta3 with Shank2 regulates mGluR-mediated calcium signal."
Hwang J.-I., Kim H.S., Lee J.R., Kim E., Ryu S.H., Suh P.-G.
J. Biol. Chem. 280:12467-12473(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SHANK2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U41411 Genomic DNA. Translation: AAC53366.1.
M99567 mRNA. Translation: AAK14906.1.
PIRA45493.
UniGeneRn.16983.

3D structure databases

ProteinModelPortalQ99JE6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-268643.

PTM databases

PhosphoSiteQ99JE6.

Proteomic databases

PaxDbQ99JE6.
PRIDEQ99JE6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:61993. rat.

Organism-specific databases

RGD61993. Plcb3.

Phylogenomic databases

eggNOGNOG149692.
HOVERGENHBG053609.
InParanoidQ99JE6.

Enzyme and pathway databases

BRENDA3.1.4.11. 5301.

Gene expression databases

GenevestigatorQ99JE6.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028390. PLC-beta3.
IPR014815. PLC-beta_C.
IPR009535. PLC-beta_CS.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF11. PTHR10336:SF11. 1 hit.
PfamPF00168. C2. 1 hit.
PF06631. DUF1154. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFPIRSF000956. PLC-beta. 1 hit.
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ99JE6.

Entry information

Entry namePLCB3_RAT
AccessionPrimary (citable) accession number: Q99JE6
Secondary accession number(s): Q62887, Q9QW29
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families