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Q99JE6

- PLCB3_RAT

UniProt

Q99JE6 - PLCB3_RAT

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

Gene

Plcb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.By similarity1 Publication

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei330 – 3301PROSITE-ProRule annotation
    Active sitei377 – 3771PROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB
    3. phospholipase C activity Source: RGD
    4. protein binding Source: RGD
    5. signal transducer activity Source: UniProtKB

    GO - Biological processi

    1. G-protein coupled receptor signaling pathway Source: RGD
    2. intracellular signal transduction Source: UniProtKB
    3. phospholipid catabolic process Source: UniProtKB
    4. post-Golgi vesicle-mediated transport Source: RGD
    5. synaptic transmission Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    BRENDAi3.1.4.11. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-beta-3
    Phospholipase C-beta-3
    Short name:
    PLC-beta-3
    Gene namesi
    Name:Plcb3
    OrganismiRattus norvegicus (Rat)Imported
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi61993. Plcb3.

    Subcellular locationi

    Cytoplasm 1 Publication. Membrane 1 Publication
    Note: And particulate fractions.

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. sarcolemma Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 123412331-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3PRO_0000088493Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei472 – 4721PhosphoserineBy similarity
    Modified residuei535 – 5351PhosphoserineBy similarity
    Modified residuei1105 – 11051PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ99JE6.
    PRIDEiQ99JE6.

    PTM databases

    PhosphoSiteiQ99JE6.

    Expressioni

    Tissue specificityi

    Expressed in parotid gland, brain, liver, uterus, lung, heart, adrenal gland, and ovary. Not detected in spleen, pancreas, intestine, thymus or kidney.1 Publication

    Gene expression databases

    GenevestigatoriQ99JE6.

    Interactioni

    Subunit structurei

    Interacts with LPAR2 By similarity. Interacts with SHANK2.By similarity1 Publication

    Protein-protein interaction databases

    MINTiMINT-268643.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99JE6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini315 – 466152PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini589 – 705117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini712 – 80998C2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1231 – 12344Interaction with SHANK2

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    HOVERGENiHBG053609.
    InParanoidiQ99JE6.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR028390. PLC-beta3.
    IPR014815. PLC-beta_C.
    IPR009535. PLC-beta_CS.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF11. PTHR10336:SF11. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF06631. DUF1154. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000956. PLC-beta. 1 hit.
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 2 hits.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99JE6-1 [UniParc]FASTAAdd to Basket

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    MAGARPGVHA LQLEPPTVVE TLRRGSKFIK WDEEASSRNL VTLRLDPNGF     50
    FLYWTGPNME VDTLDISSIR DTRTGRYARL PKDPKIREVL GFGGPDTRLE 100
    EKLMTVVAGP DPVNTTFLNF MAVQDDTVKV WSEELFKLAM NILAQNAPEH 150
    VLRKAYTKLK LQVNQDGRIP VKNILKMFSA DKKRVETALI CGLNFNRSES 200
    IRPDEFSLEI FERFLNKLLL RPDIDKILLE IGAKGKPYLT LEQLMDFINQ 250
    KQRDPRLNEV LYPPLRSSQA RLLIEKYEPN KQFLERDQMS MEGFSRYLGG 300
    EENGILPLEA LDLSMDMTQP LSAYFINSSH NTYLTAGQLA GTSSVEMYRQ 350
    ALLWGCRCVE LDVWKGRPPE EEPFITHGFT MTTEVPLRDV LEAIAETAFK 400
    TSPYPVILSF ENHVDSAKQQ AKMAEYCRSI FGEALLIDPL DKYPLSAGTP 450
    LPSPQDLMGR ILVKNKKRHR PSTGVPDSSV AKRPLEQSNS ALSESSAATE 500
    PSSPQLGSPS SDSCPGLSNG EEVGLEKTSL EPQKSLGEEG LNRGPNVLMP 550
    DRDREDEEED EEEEETTDPK KPTTDEGTAS SEVNATEEMS TLVNYVEPVK 600
    FKSFEASRKR NKCFEMSSFV ETKAMEQLTK SPMEFVEYNK QQLSRIYPKG 650
    TRVDSSNYMP QLFWNVGCQL VALNFQTLDL PMQLNAGVFE YNGRSGYLLK 700
    PEFMRRPDKS FDPFTEVIVD GIVANALRVK VISGQFLSDR KVGIYVEVDM 750
    FGLPVDTRRK YRTRTSQGNS FNPVWDEEPF DFPKVVLPTL ASLRIAAFEE 800
    GGRFVGHRIL PVSAIRSGYH YVCLRNEANQ PLCLPALLIY TEASDYIPDD 850
    HQDYAEALIN PIKHVSLMDQ RAKQLAALIG ESEAQASTEM CQETPSQQQG 900
    SQLSSNPVPN PLDDSPRWPP GPTTSPTSTS LSSPGQRDDL IASILSEVTP 950
    TPLEELRSHK AMVKLRSRQD RDLRELHKKH QRKAVALTRR LLDGLAQARA 1000
    EGKCRPSSSA LSRATNVEDV KEEEKEAARQ YREFQNRQVQ SLLELREAQA 1050
    DAETERRLEH LKQAQQRLRE VVLDAHTTQF KRLKELNERE KKELQKILDR 1100
    KRNNSISEAK TREKHKKEVE LTEINRRHIT ESVNSIRRLE EAQKQRHERL 1150
    LAGQQQVLQQ LVEEEPKLVA QLTQECQEQR ERLPQEIRRC LLGETSEGLG 1200
    DGPLVACASN GHAAGSGGHQ SGADSESQEE NTQL 1234
    Length:1,234
    Mass (Da):139,450
    Last modified:October 1, 2002 - v2
    Checksum:i2EBD7282F052CA6A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171T → A in AAK14906. (PubMed:8454637)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U41411 Genomic DNA. Translation: AAC53366.1.
    M99567 mRNA. Translation: AAK14906.1.
    PIRiA45493.
    UniGeneiRn.16983.

    Genome annotation databases

    UCSCiRGD:61993. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U41411 Genomic DNA. Translation: AAC53366.1 .
    M99567 mRNA. Translation: AAK14906.1 .
    PIRi A45493.
    UniGenei Rn.16983.

    3D structure databases

    ProteinModelPortali Q99JE6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-268643.

    PTM databases

    PhosphoSitei Q99JE6.

    Proteomic databases

    PaxDbi Q99JE6.
    PRIDEi Q99JE6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:61993. rat.

    Organism-specific databases

    RGDi 61993. Plcb3.

    Phylogenomic databases

    eggNOGi NOG149692.
    HOVERGENi HBG053609.
    InParanoidi Q99JE6.

    Enzyme and pathway databases

    BRENDAi 3.1.4.11. 5301.

    Miscellaneous databases

    PROi Q99JE6.

    Gene expression databases

    Genevestigatori Q99JE6.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR028390. PLC-beta3.
    IPR014815. PLC-beta_C.
    IPR009535. PLC-beta_CS.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF11. PTHR10336:SF11. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF06631. DUF1154. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000956. PLC-beta. 1 hit.
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 2 hits.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The 5'-upstream region of the rat phospholipase C-beta 3 gene contains two critical Sp1 sites and an HIV Inr-like element."
      Kang J.S., Lee H.B., Rhee S.G., Park K., Yoo O.J.
      Gene 197:19-28(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33.
    2. "Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3."
      Jhon D.-Y., Lee H.-H., Park D., Lee C.-W., Lee K.-H., Yoo O.J., Rhee S.G.
      J. Biol. Chem. 268:6654-6661(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-1234, FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: Fischer.
      Tissue: Thyroid.
    3. "The interaction of phospholipase C-beta3 with Shank2 regulates mGluR-mediated calcium signal."
      Hwang J.-I., Kim H.S., Lee J.R., Kim E., Ryu S.H., Suh P.-G.
      J. Biol. Chem. 280:12467-12473(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SHANK2.

    Entry informationi

    Entry nameiPLCB3_RAT
    AccessioniPrimary (citable) accession number: Q99JE6
    Secondary accession number(s): Q62887, Q9QW29
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2003
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3