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Q99JD4

- CLAP2_RAT

UniProt

Q99JD4 - CLAP2_RAT

Protein

CLIP-associating protein 2

Gene

Clasp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex By similarity.By similarity

    GO - Molecular functioni

    1. microtubule binding Source: RGD
    2. microtubule plus-end binding Source: UniProtKB

    GO - Biological processi

    1. cell migration Source: Ensembl
    2. establishment or maintenance of cell polarity Source: Ensembl
    3. microtubule anchoring Source: UniProtKB
    4. microtubule nucleation Source: UniProtKB
    5. microtubule organizing center organization Source: UniProtKB
    6. mitotic nuclear division Source: UniProtKB-KW
    7. negative regulation of microtubule depolymerization Source: RGD
    8. regulation of microtubule-based process Source: UniProtKB

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_194781. Separation of Sister Chromatids.
    REACT_198611. Resolution of Sister Chromatid Cohesion.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CLIP-associating protein 2
    Alternative name(s):
    Cytoplasmic linker-associated protein 2
    Gene namesi
    Name:Clasp2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi619789. Clasp2.

    Subcellular locationi

    Cytoplasmcytoskeleton. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Chromosomecentromerekinetochore. Cytoplasmcytoskeletonspindle. Golgi apparatus By similarity. Golgi apparatustrans-Golgi network By similarity. Cell membrane By similarity. Cell projectionruffle membrane By similarity
    Note: Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane By similarity.By similarity

    GO - Cellular componenti

    1. condensed chromosome kinetochore Source: UniProtKB-SubCell
    2. cytoplasmic microtubule Source: Ensembl
    3. microtubule Source: RGD
    4. microtubule organizing center Source: UniProtKB-SubCell
    5. plasma membrane Source: UniProtKB
    6. ruffle membrane Source: UniProtKB
    7. spindle Source: UniProtKB-SubCell
    8. trans-Golgi network Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Centromere, Chromosome, Cytoplasm, Cytoskeleton, Golgi apparatus, Kinetochore, Membrane, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12861286CLIP-associating protein 2PRO_0000089851Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei14 – 141PhosphoserineBy similarity
    Modified residuei376 – 3761PhosphoserineBy similarity
    Modified residuei461 – 4611PhosphoserineBy similarity
    Modified residuei531 – 5311PhosphoserineBy similarity
    Modified residuei535 – 5351PhosphoserineBy similarity
    Modified residuei581 – 5811PhosphoserineBy similarity
    Modified residuei1021 – 10211PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by GSK3B. Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ99JD4.
    PRIDEiQ99JD4.

    PTM databases

    PhosphoSiteiQ99JD4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ99JD4.

    Interactioni

    Subunit structurei

    Interacts with CLIP2, ERC1, MAPRE3, microtubules, PHLDB2 and RSN. The interaction with ERC1 may be mediated by PHLDB2. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with GCC2; recruits CLASP2 to Golgi membranes By similarity. Interacts with MACF1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi250360. 1 interaction.
    MINTiMINT-4579893.
    STRINGi10116.ENSRNOP00000063775.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99JD4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati179 – 21436HEAT 1Add
    BLAST
    Repeati215 – 25137HEAT 2Add
    BLAST
    Repeati764 – 80138HEAT 3Add
    BLAST
    Repeati1090 – 112738HEAT 4Add
    BLAST
    Repeati1208 – 124538HEAT 5Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4040Golgi localizationBy similarityAdd
    BLAST
    Regioni450 – 565116Interaction with microtubules, MAPRE1 and MAPRE3By similarityAdd
    BLAST
    Regioni864 – 1286423Interaction with RSN and localization to the Golgi and kinetochoresBy similarityAdd
    BLAST
    Regioni1009 – 1286278Required for cortical localizationBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1047 – 107630Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi500 – 5034Microtubule tip localization signal
    Motifi523 – 5264Microtubule tip localization signal

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi316 – 572257Ser-richAdd
    BLAST
    Compositional biasi1096 – 11005Poly-Leu

    Domaini

    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.By similarity

    Sequence similaritiesi

    Belongs to the CLASP family.Curated
    Contains 5 HEAT repeats.Curated

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiNOG81443.
    GeneTreeiENSGT00390000001762.
    HOGENOMiHOG000236347.
    HOVERGENiHBG079692.
    InParanoidiQ99JD4.
    KOiK16578.
    OMAiPLAGMGN.
    OrthoDBiEOG780RKK.

    Family and domain databases

    Gene3Di1.25.10.10. 3 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR024395. CLASP_N_dom.
    [Graphical view]
    PfamiPF12348. CLASP_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Q99JD4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRLICKRIC DYKSFDDEES VDGNRPSSAA SAFKVPAPKT PGNPVNSARK     50
    PGSAGGPKAG GTSKEGGAGA VDEDDFIKAF TDVPSIQIYS SRELEETLNK 100
    IREILSDDKH DWDQRANALK KIRSLLVAGA AQYDCFFQHL RLLDGALKLS 150
    AKDLRSQVVR EACITVAHLS TVLGNKFDHG AEAIVPTLFN LVPNSAKVMA 200
    TSGCAAIRFI IRHTHVPRLI PLITSNCTSK SVPVRRRSFE FLDLLLQEWQ 250
    THSLERHAAV LVETIKKGIH DADAEARVEA RKTYMGLRNH FPGEAETLYN 300
    SLEPSYQKSL QTYLKSSGSV ASLPQSDRSS SSSQESLNRP FSSKWSTANP 350
    SAVAGRVSVG GSKASPLPGS LQRSRSDIDV NAAAGAKAHH AAGQAVRSGR 400
    LGAGALNPGS YASLEDTSDK MDGTASEDGR VRAKLSTPLV AVGNAKTDSR 450
    GRSRTKMVSQ SQPGSRSGSP GRVLTTTALS TVSSGAQRIL VNSASAQKRS 500
    KIPRSQGCSR EASPSRLSVA RSSRIPRPSV SQGCSREASR ESSRDTSPVR 550
    SFQPLGPGYG MSQSSRLSSS VSAMRVLNTG SDVEEAVADA LLLGDIRTKK 600
    KPARRRYESY GMHSDDDANS DASSACSERS YSSRNGSIPT YMRQTEDVAE 650
    VLNRCASSNW SERKEGLLGL QNLLKNQRTL SRVELKRLCE IFTRMFADPH 700
    GKVFSMFLET LVDFIQVHKD DLQDWLFVLL TQLLKKMGAD LLGSVQAKVQ 750
    KALDVTRESF PNDLQFNILM RFTVDQTQTP SLKVKVAILK YIETLAKQMD 800
    PGDFINSSET RLAVSRVITW TTEPKSSDVR KAAQSVLISL FELNTPEFTM 850
    LLGALPKTFQ DGATKLLHNH LRNTGNGTQS SMGSPLTRPT PRSPANWSSP 900
    LTSPTNTSQN TLSPSAFDYD TENMNSEDIY SSLRGVTEAI QNFSFRSQED 950
    MSEPLKRDPK KEDGDTVCSG PGMSDPRAGG DAPDSSQPAL DNKASLLHSV 1000
    PLHSSPRSRD YNPYNYSDSI SPFNKSALKE AMFDDDADQF PDDLSLDHSD 1050
    LVAELLKELS NHNERIEERK IALYELMKLT QEESFSVWDE HFKTILLLLL 1100
    ETLGDKEPTI RALALKVLKE ILRHQPARFK NYAELTVMKT LEAHKDPHKE 1150
    VVRSAEEAAS VLATSISPEQ CIKVLCPIIQ TADYPINLAA IKMQTKVIER 1200
    VSKETLNLLL PEIMPGLIQG YDNSESSVRK ACVFCLVAVH AVIGDELKPH 1250
    LSQLTGSKMK LLNLYIKRAQ TGSAGADPTT DVSGQS 1286
    Length:1,286
    Mass (Da):140,638
    Last modified:June 1, 2001 - v1
    Checksum:iC1742635DADC3F09
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ288060 mRNA. Translation: CAC35166.1.
    RefSeqiNP_446174.1. NM_053722.2.
    UniGeneiRn.19943.

    Genome annotation databases

    EnsembliENSRNOT00000012545; ENSRNOP00000012545; ENSRNOG00000009161.
    GeneIDi114514.
    KEGGirno:114514.
    UCSCiRGD:619789. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ288060 mRNA. Translation: CAC35166.1 .
    RefSeqi NP_446174.1. NM_053722.2.
    UniGenei Rn.19943.

    3D structure databases

    ProteinModelPortali Q99JD4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250360. 1 interaction.
    MINTi MINT-4579893.
    STRINGi 10116.ENSRNOP00000063775.

    PTM databases

    PhosphoSitei Q99JD4.

    Proteomic databases

    PaxDbi Q99JD4.
    PRIDEi Q99JD4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000012545 ; ENSRNOP00000012545 ; ENSRNOG00000009161 .
    GeneIDi 114514.
    KEGGi rno:114514.
    UCSCi RGD:619789. rat.

    Organism-specific databases

    CTDi 23122.
    RGDi 619789. Clasp2.

    Phylogenomic databases

    eggNOGi NOG81443.
    GeneTreei ENSGT00390000001762.
    HOGENOMi HOG000236347.
    HOVERGENi HBG079692.
    InParanoidi Q99JD4.
    KOi K16578.
    OMAi PLAGMGN.
    OrthoDBi EOG780RKK.

    Enzyme and pathway databases

    Reactomei REACT_194781. Separation of Sister Chromatids.
    REACT_198611. Resolution of Sister Chromatid Cohesion.

    Miscellaneous databases

    NextBioi 618631.
    PROi Q99JD4.

    Gene expression databases

    Genevestigatori Q99JD4.

    Family and domain databases

    Gene3Di 1.25.10.10. 3 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR024395. CLASP_N_dom.
    [Graphical view ]
    Pfami PF12348. CLASP_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts."
      Akhmanova A., Hoogenraad C.C., Drabek K., Stepanova T., Dortland B., Verkerk T., Vermeulen W., Burgering B.M., de Zeeuw C.I., Grosveld F., Galjart N.
      Cell 104:923-935(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiCLAP2_RAT
    AccessioniPrimary (citable) accession number: Q99JD4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3