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Q99JD4

- CLAP2_RAT

UniProt

Q99JD4 - CLAP2_RAT

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Protein
CLIP-associating protein 2
Gene
Clasp2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex By similarity.

GO - Molecular functioni

  1. microtubule binding Source: RGD
  2. microtubule plus-end binding Source: UniProtKB

GO - Biological processi

  1. cell migration Source: Ensembl
  2. establishment or maintenance of cell polarity Source: Ensembl
  3. microtubule anchoring Source: UniProtKB
  4. microtubule nucleation Source: UniProtKB
  5. microtubule organizing center organization Source: UniProtKB
  6. mitotic nuclear division Source: UniProtKB-KW
  7. negative regulation of microtubule depolymerization Source: RGD
  8. regulation of microtubule-based process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_194781. Separation of Sister Chromatids.
REACT_198611. Resolution of Sister Chromatid Cohesion.

Names & Taxonomyi

Protein namesi
Recommended name:
CLIP-associating protein 2
Alternative name(s):
Cytoplasmic linker-associated protein 2
Gene namesi
Name:Clasp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi619789. Clasp2.

Subcellular locationi

Cytoplasmcytoskeleton. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Chromosomecentromerekinetochore. Cytoplasmcytoskeletonspindle. Golgi apparatus By similarity. Golgi apparatustrans-Golgi network By similarity. Cell membrane By similarity. Cell projectionruffle membrane By similarity
Note: Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane By similarity.

GO - Cellular componenti

  1. condensed chromosome kinetochore Source: UniProtKB-SubCell
  2. cytoplasmic microtubule Source: Ensembl
  3. microtubule Source: RGD
  4. microtubule organizing center Source: UniProtKB-SubCell
  5. plasma membrane Source: UniProtKB
  6. ruffle membrane Source: UniProtKB
  7. spindle Source: UniProtKB-SubCell
  8. trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Centromere, Chromosome, Cytoplasm, Cytoskeleton, Golgi apparatus, Kinetochore, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12861286CLIP-associating protein 2
PRO_0000089851Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141Phosphoserine By similarity
Modified residuei376 – 3761Phosphoserine By similarity
Modified residuei461 – 4611Phosphoserine By similarity
Modified residuei531 – 5311Phosphoserine By similarity
Modified residuei535 – 5351Phosphoserine By similarity
Modified residuei581 – 5811Phosphoserine By similarity
Modified residuei1021 – 10211Phosphoserine By similarity

Post-translational modificationi

Phosphorylated by GSK3B. Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99JD4.
PRIDEiQ99JD4.

PTM databases

PhosphoSiteiQ99JD4.

Expressioni

Gene expression databases

GenevestigatoriQ99JD4.

Interactioni

Subunit structurei

Interacts with CLIP2, ERC1, MAPRE3, microtubules, PHLDB2 and RSN. The interaction with ERC1 may be mediated by PHLDB2. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with GCC2; recruits CLASP2 to Golgi membranes By similarity. Interacts with MACF1 By similarity.

Protein-protein interaction databases

BioGridi250360. 1 interaction.
MINTiMINT-4579893.
STRINGi10116.ENSRNOP00000063775.

Structurei

3D structure databases

ProteinModelPortaliQ99JD4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati179 – 21436HEAT 1
Add
BLAST
Repeati215 – 25137HEAT 2
Add
BLAST
Repeati764 – 80138HEAT 3
Add
BLAST
Repeati1090 – 112738HEAT 4
Add
BLAST
Repeati1208 – 124538HEAT 5
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4040Golgi localization By similarity
Add
BLAST
Regioni450 – 565116Interaction with microtubules, MAPRE1 and MAPRE3 By similarity
Add
BLAST
Regioni864 – 1286423Interaction with RSN and localization to the Golgi and kinetochores By similarity
Add
BLAST
Regioni1009 – 1286278Required for cortical localization By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1047 – 107630 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi500 – 5034Microtubule tip localization signal
Motifi523 – 5264Microtubule tip localization signal

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi316 – 572257Ser-rich
Add
BLAST
Compositional biasi1096 – 11005Poly-Leu

Domaini

The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends By similarity.

Sequence similaritiesi

Belongs to the CLASP family.
Contains 5 HEAT repeats.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG81443.
GeneTreeiENSGT00390000001762.
HOGENOMiHOG000236347.
HOVERGENiHBG079692.
InParanoidiQ99JD4.
KOiK16578.
OMAiPLAGMGN.
OrthoDBiEOG780RKK.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024395. CLASP_N_dom.
[Graphical view]
PfamiPF12348. CLASP_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

Q99JD4-1 [UniParc]FASTAAdd to Basket

« Hide

MRRLICKRIC DYKSFDDEES VDGNRPSSAA SAFKVPAPKT PGNPVNSARK     50
PGSAGGPKAG GTSKEGGAGA VDEDDFIKAF TDVPSIQIYS SRELEETLNK 100
IREILSDDKH DWDQRANALK KIRSLLVAGA AQYDCFFQHL RLLDGALKLS 150
AKDLRSQVVR EACITVAHLS TVLGNKFDHG AEAIVPTLFN LVPNSAKVMA 200
TSGCAAIRFI IRHTHVPRLI PLITSNCTSK SVPVRRRSFE FLDLLLQEWQ 250
THSLERHAAV LVETIKKGIH DADAEARVEA RKTYMGLRNH FPGEAETLYN 300
SLEPSYQKSL QTYLKSSGSV ASLPQSDRSS SSSQESLNRP FSSKWSTANP 350
SAVAGRVSVG GSKASPLPGS LQRSRSDIDV NAAAGAKAHH AAGQAVRSGR 400
LGAGALNPGS YASLEDTSDK MDGTASEDGR VRAKLSTPLV AVGNAKTDSR 450
GRSRTKMVSQ SQPGSRSGSP GRVLTTTALS TVSSGAQRIL VNSASAQKRS 500
KIPRSQGCSR EASPSRLSVA RSSRIPRPSV SQGCSREASR ESSRDTSPVR 550
SFQPLGPGYG MSQSSRLSSS VSAMRVLNTG SDVEEAVADA LLLGDIRTKK 600
KPARRRYESY GMHSDDDANS DASSACSERS YSSRNGSIPT YMRQTEDVAE 650
VLNRCASSNW SERKEGLLGL QNLLKNQRTL SRVELKRLCE IFTRMFADPH 700
GKVFSMFLET LVDFIQVHKD DLQDWLFVLL TQLLKKMGAD LLGSVQAKVQ 750
KALDVTRESF PNDLQFNILM RFTVDQTQTP SLKVKVAILK YIETLAKQMD 800
PGDFINSSET RLAVSRVITW TTEPKSSDVR KAAQSVLISL FELNTPEFTM 850
LLGALPKTFQ DGATKLLHNH LRNTGNGTQS SMGSPLTRPT PRSPANWSSP 900
LTSPTNTSQN TLSPSAFDYD TENMNSEDIY SSLRGVTEAI QNFSFRSQED 950
MSEPLKRDPK KEDGDTVCSG PGMSDPRAGG DAPDSSQPAL DNKASLLHSV 1000
PLHSSPRSRD YNPYNYSDSI SPFNKSALKE AMFDDDADQF PDDLSLDHSD 1050
LVAELLKELS NHNERIEERK IALYELMKLT QEESFSVWDE HFKTILLLLL 1100
ETLGDKEPTI RALALKVLKE ILRHQPARFK NYAELTVMKT LEAHKDPHKE 1150
VVRSAEEAAS VLATSISPEQ CIKVLCPIIQ TADYPINLAA IKMQTKVIER 1200
VSKETLNLLL PEIMPGLIQG YDNSESSVRK ACVFCLVAVH AVIGDELKPH 1250
LSQLTGSKMK LLNLYIKRAQ TGSAGADPTT DVSGQS 1286
Length:1,286
Mass (Da):140,638
Last modified:June 1, 2001 - v1
Checksum:iC1742635DADC3F09
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ288060 mRNA. Translation: CAC35166.1.
RefSeqiNP_446174.1. NM_053722.2.
UniGeneiRn.19943.

Genome annotation databases

EnsembliENSRNOT00000012545; ENSRNOP00000012545; ENSRNOG00000009161.
GeneIDi114514.
KEGGirno:114514.
UCSCiRGD:619789. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ288060 mRNA. Translation: CAC35166.1 .
RefSeqi NP_446174.1. NM_053722.2.
UniGenei Rn.19943.

3D structure databases

ProteinModelPortali Q99JD4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250360. 1 interaction.
MINTi MINT-4579893.
STRINGi 10116.ENSRNOP00000063775.

PTM databases

PhosphoSitei Q99JD4.

Proteomic databases

PaxDbi Q99JD4.
PRIDEi Q99JD4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000012545 ; ENSRNOP00000012545 ; ENSRNOG00000009161 .
GeneIDi 114514.
KEGGi rno:114514.
UCSCi RGD:619789. rat.

Organism-specific databases

CTDi 23122.
RGDi 619789. Clasp2.

Phylogenomic databases

eggNOGi NOG81443.
GeneTreei ENSGT00390000001762.
HOGENOMi HOG000236347.
HOVERGENi HBG079692.
InParanoidi Q99JD4.
KOi K16578.
OMAi PLAGMGN.
OrthoDBi EOG780RKK.

Enzyme and pathway databases

Reactomei REACT_194781. Separation of Sister Chromatids.
REACT_198611. Resolution of Sister Chromatid Cohesion.

Miscellaneous databases

NextBioi 618631.
PROi Q99JD4.

Gene expression databases

Genevestigatori Q99JD4.

Family and domain databases

Gene3Di 1.25.10.10. 3 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024395. CLASP_N_dom.
[Graphical view ]
Pfami PF12348. CLASP_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 3 hits.
ProtoNeti Search...

Publicationsi

  1. "Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts."
    Akhmanova A., Hoogenraad C.C., Drabek K., Stepanova T., Dortland B., Verkerk T., Vermeulen W., Burgering B.M., de Zeeuw C.I., Grosveld F., Galjart N.
    Cell 104:923-935(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiCLAP2_RAT
AccessioniPrimary (citable) accession number: Q99JD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi