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Q99JD4

- CLAP2_RAT

UniProt

Q99JD4 - CLAP2_RAT

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Protein

CLIP-associating protein 2

Gene

Clasp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex (By similarity).By similarity

GO - Molecular functioni

  1. microtubule binding Source: RGD
  2. microtubule plus-end binding Source: UniProtKB

GO - Biological processi

  1. cell migration Source: Ensembl
  2. establishment or maintenance of cell polarity Source: Ensembl
  3. microtubule anchoring Source: UniProtKB
  4. microtubule nucleation Source: UniProtKB
  5. microtubule organizing center organization Source: UniProtKB
  6. mitotic nuclear division Source: UniProtKB-KW
  7. negative regulation of microtubule depolymerization Source: RGD
  8. regulation of microtubule-based process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_194781. Separation of Sister Chromatids.
REACT_198611. Resolution of Sister Chromatid Cohesion.

Names & Taxonomyi

Protein namesi
Recommended name:
CLIP-associating protein 2
Alternative name(s):
Cytoplasmic linker-associated protein 2
Gene namesi
Name:Clasp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi619789. Clasp2.

Subcellular locationi

Cytoplasmcytoskeleton. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Chromosomecentromerekinetochore. Cytoplasmcytoskeletonspindle. Golgi apparatus By similarity. Golgi apparatustrans-Golgi network By similarity. Cell membrane By similarity. Cell projectionruffle membrane By similarity
Note: Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasmic microtubule Source: Ensembl
  2. kinetochore Source: UniProtKB-KW
  3. microtubule Source: RGD
  4. plasma membrane Source: UniProtKB
  5. ruffle membrane Source: UniProtKB
  6. trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Centromere, Chromosome, Cytoplasm, Cytoskeleton, Golgi apparatus, Kinetochore, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12861286CLIP-associating protein 2PRO_0000089851Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei376 – 3761PhosphoserineBy similarity
Modified residuei461 – 4611PhosphoserineBy similarity
Modified residuei531 – 5311PhosphoserineBy similarity
Modified residuei535 – 5351PhosphoserineBy similarity
Modified residuei581 – 5811PhosphoserineBy similarity
Modified residuei1021 – 10211PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by GSK3B. Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99JD4.
PRIDEiQ99JD4.

PTM databases

PhosphoSiteiQ99JD4.

Expressioni

Gene expression databases

GenevestigatoriQ99JD4.

Interactioni

Subunit structurei

Interacts with CLIP2, ERC1, MAPRE3, microtubules, PHLDB2 and RSN. The interaction with ERC1 may be mediated by PHLDB2. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with GCC2; recruits CLASP2 to Golgi membranes (By similarity). Interacts with MACF1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi250360. 1 interaction.
MINTiMINT-4579893.
STRINGi10116.ENSRNOP00000063775.

Structurei

3D structure databases

ProteinModelPortaliQ99JD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati179 – 21436HEAT 1Add
BLAST
Repeati215 – 25137HEAT 2Add
BLAST
Repeati764 – 80138HEAT 3Add
BLAST
Repeati1090 – 112738HEAT 4Add
BLAST
Repeati1208 – 124538HEAT 5Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4040Golgi localizationBy similarityAdd
BLAST
Regioni450 – 565116Interaction with microtubules, MAPRE1 and MAPRE3By similarityAdd
BLAST
Regioni864 – 1286423Interaction with RSN and localization to the Golgi and kinetochoresBy similarityAdd
BLAST
Regioni1009 – 1286278Required for cortical localizationBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1047 – 107630Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi500 – 5034Microtubule tip localization signal
Motifi523 – 5264Microtubule tip localization signal

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi316 – 572257Ser-richAdd
BLAST
Compositional biasi1096 – 11005Poly-Leu

Domaini

The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.By similarity

Sequence similaritiesi

Belongs to the CLASP family.Curated
Contains 5 HEAT repeats.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG81443.
GeneTreeiENSGT00390000001762.
HOGENOMiHOG000236347.
HOVERGENiHBG079692.
InParanoidiQ99JD4.
KOiK16578.
OMAiPLAGMGN.
OrthoDBiEOG780RKK.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024395. CLASP_N_dom.
[Graphical view]
PfamiPF12348. CLASP_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

Q99JD4 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRRLICKRIC DYKSFDDEES VDGNRPSSAA SAFKVPAPKT PGNPVNSARK
60 70 80 90 100
PGSAGGPKAG GTSKEGGAGA VDEDDFIKAF TDVPSIQIYS SRELEETLNK
110 120 130 140 150
IREILSDDKH DWDQRANALK KIRSLLVAGA AQYDCFFQHL RLLDGALKLS
160 170 180 190 200
AKDLRSQVVR EACITVAHLS TVLGNKFDHG AEAIVPTLFN LVPNSAKVMA
210 220 230 240 250
TSGCAAIRFI IRHTHVPRLI PLITSNCTSK SVPVRRRSFE FLDLLLQEWQ
260 270 280 290 300
THSLERHAAV LVETIKKGIH DADAEARVEA RKTYMGLRNH FPGEAETLYN
310 320 330 340 350
SLEPSYQKSL QTYLKSSGSV ASLPQSDRSS SSSQESLNRP FSSKWSTANP
360 370 380 390 400
SAVAGRVSVG GSKASPLPGS LQRSRSDIDV NAAAGAKAHH AAGQAVRSGR
410 420 430 440 450
LGAGALNPGS YASLEDTSDK MDGTASEDGR VRAKLSTPLV AVGNAKTDSR
460 470 480 490 500
GRSRTKMVSQ SQPGSRSGSP GRVLTTTALS TVSSGAQRIL VNSASAQKRS
510 520 530 540 550
KIPRSQGCSR EASPSRLSVA RSSRIPRPSV SQGCSREASR ESSRDTSPVR
560 570 580 590 600
SFQPLGPGYG MSQSSRLSSS VSAMRVLNTG SDVEEAVADA LLLGDIRTKK
610 620 630 640 650
KPARRRYESY GMHSDDDANS DASSACSERS YSSRNGSIPT YMRQTEDVAE
660 670 680 690 700
VLNRCASSNW SERKEGLLGL QNLLKNQRTL SRVELKRLCE IFTRMFADPH
710 720 730 740 750
GKVFSMFLET LVDFIQVHKD DLQDWLFVLL TQLLKKMGAD LLGSVQAKVQ
760 770 780 790 800
KALDVTRESF PNDLQFNILM RFTVDQTQTP SLKVKVAILK YIETLAKQMD
810 820 830 840 850
PGDFINSSET RLAVSRVITW TTEPKSSDVR KAAQSVLISL FELNTPEFTM
860 870 880 890 900
LLGALPKTFQ DGATKLLHNH LRNTGNGTQS SMGSPLTRPT PRSPANWSSP
910 920 930 940 950
LTSPTNTSQN TLSPSAFDYD TENMNSEDIY SSLRGVTEAI QNFSFRSQED
960 970 980 990 1000
MSEPLKRDPK KEDGDTVCSG PGMSDPRAGG DAPDSSQPAL DNKASLLHSV
1010 1020 1030 1040 1050
PLHSSPRSRD YNPYNYSDSI SPFNKSALKE AMFDDDADQF PDDLSLDHSD
1060 1070 1080 1090 1100
LVAELLKELS NHNERIEERK IALYELMKLT QEESFSVWDE HFKTILLLLL
1110 1120 1130 1140 1150
ETLGDKEPTI RALALKVLKE ILRHQPARFK NYAELTVMKT LEAHKDPHKE
1160 1170 1180 1190 1200
VVRSAEEAAS VLATSISPEQ CIKVLCPIIQ TADYPINLAA IKMQTKVIER
1210 1220 1230 1240 1250
VSKETLNLLL PEIMPGLIQG YDNSESSVRK ACVFCLVAVH AVIGDELKPH
1260 1270 1280
LSQLTGSKMK LLNLYIKRAQ TGSAGADPTT DVSGQS
Length:1,286
Mass (Da):140,638
Last modified:June 1, 2001 - v1
Checksum:iC1742635DADC3F09
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ288060 mRNA. Translation: CAC35166.1.
RefSeqiNP_446174.1. NM_053722.2.
UniGeneiRn.19943.

Genome annotation databases

EnsembliENSRNOT00000012545; ENSRNOP00000012545; ENSRNOG00000009161.
GeneIDi114514.
KEGGirno:114514.
UCSCiRGD:619789. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ288060 mRNA. Translation: CAC35166.1 .
RefSeqi NP_446174.1. NM_053722.2.
UniGenei Rn.19943.

3D structure databases

ProteinModelPortali Q99JD4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250360. 1 interaction.
MINTi MINT-4579893.
STRINGi 10116.ENSRNOP00000063775.

PTM databases

PhosphoSitei Q99JD4.

Proteomic databases

PaxDbi Q99JD4.
PRIDEi Q99JD4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000012545 ; ENSRNOP00000012545 ; ENSRNOG00000009161 .
GeneIDi 114514.
KEGGi rno:114514.
UCSCi RGD:619789. rat.

Organism-specific databases

CTDi 23122.
RGDi 619789. Clasp2.

Phylogenomic databases

eggNOGi NOG81443.
GeneTreei ENSGT00390000001762.
HOGENOMi HOG000236347.
HOVERGENi HBG079692.
InParanoidi Q99JD4.
KOi K16578.
OMAi PLAGMGN.
OrthoDBi EOG780RKK.

Enzyme and pathway databases

Reactomei REACT_194781. Separation of Sister Chromatids.
REACT_198611. Resolution of Sister Chromatid Cohesion.

Miscellaneous databases

NextBioi 618631.
PROi Q99JD4.

Gene expression databases

Genevestigatori Q99JD4.

Family and domain databases

Gene3Di 1.25.10.10. 3 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024395. CLASP_N_dom.
[Graphical view ]
Pfami PF12348. CLASP_N. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 3 hits.
ProtoNeti Search...

Publicationsi

  1. "Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts."
    Akhmanova A., Hoogenraad C.C., Drabek K., Stepanova T., Dortland B., Verkerk T., Vermeulen W., Burgering B.M., de Zeeuw C.I., Grosveld F., Galjart N.
    Cell 104:923-935(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiCLAP2_RAT
AccessioniPrimary (citable) accession number: Q99JD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3