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Protein

Protein kinase C and casein kinase II substrate protein 3

Gene

Pacsin3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.4 Publications

GO - Molecular functioni

  • calcium channel inhibitor activity Source: UniProtKB
  • cytoskeletal protein binding Source: MGI
  • lipid binding Source: UniProtKB

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • membrane tubulation Source: UniProtKB
  • negative regulation of calcium ion transport Source: UniProtKB
  • negative regulation of endocytosis Source: UniProtKB
  • positive regulation of membrane protein ectodomain proteolysis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase II substrate protein 3
Gene namesi
Name:Pacsin3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1891410. Pacsin3.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

  • Note: Detected at the inner aspect of the plasma membrane in myotubes.

GO - Cellular componenti

  • cytoplasm Source: MGI
  • endosome Source: GO_Central
  • extracellular exosome Source: MGI
  • nucleoplasm Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi415P → L: Loss of DNM1-, SYNJ1- and WASL-binding. Loss of effect on transferrin endocytosis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001618011 – 424Protein kinase C and casein kinase II substrate protein 3Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276PhosphoserineBy similarity1
Modified residuei303PhosphoserineCombined sources1
Modified residuei319PhosphoserineCombined sources1
Modified residuei324PhosphothreonineBy similarity1
Modified residuei327PhosphoserineBy similarity1
Modified residuei354PhosphoserineCombined sources1
Modified residuei383PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99JB8.
PaxDbiQ99JB8.
PRIDEiQ99JB8.

PTM databases

iPTMnetiQ99JB8.
PhosphoSitePlusiQ99JB8.
SwissPalmiQ99JB8.

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle, heart and lung; also detected in brain, kidney and uterus (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000027257.
ExpressionAtlasiQ99JB8. baseline and differential.
GenevisibleiQ99JB8. MM.

Interactioni

Subunit structurei

Homodimer. May form heterooligomers with other PACSINs. Interacts (via SH3 domain) with DNM1, SYNJ1 and WASL. Interacts with TRPV4.4 Publications

GO - Molecular functioni

  • cytoskeletal protein binding Source: MGI

Protein-protein interaction databases

IntActiQ99JB8. 4 interactors.
MINTiMINT-1850676.
STRINGi10090.ENSMUSP00000028694.

Structurei

Secondary structure

1424
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni19 – 22Combined sources4
Helixi23 – 70Combined sources48
Helixi75 – 103Combined sources29
Helixi105 – 117Combined sources13
Beta strandi122 – 126Combined sources5
Helixi127 – 170Combined sources44
Beta strandi186 – 190Combined sources5
Turni191 – 193Combined sources3
Helixi194 – 253Combined sources60
Helixi255 – 257Combined sources3
Helixi259 – 274Combined sources16
Helixi277 – 288Combined sources12
Beta strandi289 – 292Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M3WX-ray2.60A/B1-320[»]
3QE6X-ray2.60A/B1-304[»]
3SYVX-ray3.10A/B/C/D/E/F/G/H1-341[»]
ProteinModelPortaliQ99JB8.
SMRiQ99JB8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 280F-BARPROSITE-ProRule annotationAdd BLAST271
Domaini363 – 424SH3PROSITE-ProRule annotationAdd BLAST62

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili23 – 2721 PublicationAdd BLAST250

Domaini

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation.1 Publication

Sequence similaritiesi

Belongs to the PACSIN family.Curated
Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG2856. Eukaryota.
ENOG410XRX2. LUCA.
GeneTreeiENSGT00510000046376.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
InParanoidiQ99JB8.
KOiK20123.
OMAiAFESCQA.
OrthoDBiEOG091G0AS9.
PhylomeDBiQ99JB8.
TreeFamiTF313677.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR028523. PACSIN3.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23065:SF18. PTHR23065:SF18. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99JB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPEEDAGGE VLGGSFWEAG NYRRTVQRVE DGHRLCGDLV SCFQERARIE
60 70 80 90 100
KAYAQQLADW ARKWRGAVEK GPQYGTLEKA WHAFFTAAER LSELHLEVRE
110 120 130 140 150
KLHGPDSERV RTWQRGAFHR PVLGGFRESR AAEDGFRKAQ KPWLKRLKEV
160 170 180 190 200
EASKKSYHTA RKDEKTAQTR ESHAKADSSM SQEQLRKLQE RVGRCTKEAE
210 220 230 240 250
KMKTQYEQTL AELNRYTPRY MEDMEQAFES CQAAERQRLL FFKDVLLTLH
260 270 280 290 300
QHLDLSSSDK FHELHRDLQQ SIEAASDEED LRWWRSTHGP GMAMNWPQFE
310 320 330 340 350
EWSLDTQRAI SRKEKGGRSP DEVTLTSIVP TRDGTAPPPQ SPSSPGSGQD
360 370 380 390 400
EDWSDEESPR KVATGVRVRA LYDYAGQEAD ELSFRAGEEL LKMSEEDEQG
410 420
WCQGQLQSGR IGLYPANYVE CVGA
Length:424
Mass (Da):48,585
Last modified:June 1, 2001 - v1
Checksum:i00475BC0321485B7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti360R → G in AAG31022 (PubMed:11082044).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149824 mRNA. Translation: AAG31022.1.
AF242531 mRNA. Translation: AAK29208.1.
BC003884 mRNA. Translation: AAH03884.1.
CCDSiCCDS16429.1.
RefSeqiNP_001276606.1. NM_001289677.1.
NP_001276607.1. NM_001289678.1.
NP_083009.1. NM_028733.4.
NP_112019.2. NM_030880.3.
XP_006500487.1. XM_006500424.3.
XP_006500488.1. XM_006500425.1.
XP_006500490.1. XM_006500427.3.
XP_006500491.1. XM_006500428.3.
UniGeneiMm.236650.

Genome annotation databases

EnsembliENSMUST00000028694; ENSMUSP00000028694; ENSMUSG00000027257.
ENSMUST00000059566; ENSMUSP00000054391; ENSMUSG00000027257.
ENSMUST00000111349; ENSMUSP00000106981; ENSMUSG00000027257.
ENSMUST00000168916; ENSMUSP00000129175; ENSMUSG00000027257.
GeneIDi80708.
KEGGimmu:80708.
UCSCiuc008kvm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149824 mRNA. Translation: AAG31022.1.
AF242531 mRNA. Translation: AAK29208.1.
BC003884 mRNA. Translation: AAH03884.1.
CCDSiCCDS16429.1.
RefSeqiNP_001276606.1. NM_001289677.1.
NP_001276607.1. NM_001289678.1.
NP_083009.1. NM_028733.4.
NP_112019.2. NM_030880.3.
XP_006500487.1. XM_006500424.3.
XP_006500488.1. XM_006500425.1.
XP_006500490.1. XM_006500427.3.
XP_006500491.1. XM_006500428.3.
UniGeneiMm.236650.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M3WX-ray2.60A/B1-320[»]
3QE6X-ray2.60A/B1-304[»]
3SYVX-ray3.10A/B/C/D/E/F/G/H1-341[»]
ProteinModelPortaliQ99JB8.
SMRiQ99JB8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99JB8. 4 interactors.
MINTiMINT-1850676.
STRINGi10090.ENSMUSP00000028694.

PTM databases

iPTMnetiQ99JB8.
PhosphoSitePlusiQ99JB8.
SwissPalmiQ99JB8.

Proteomic databases

MaxQBiQ99JB8.
PaxDbiQ99JB8.
PRIDEiQ99JB8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028694; ENSMUSP00000028694; ENSMUSG00000027257.
ENSMUST00000059566; ENSMUSP00000054391; ENSMUSG00000027257.
ENSMUST00000111349; ENSMUSP00000106981; ENSMUSG00000027257.
ENSMUST00000168916; ENSMUSP00000129175; ENSMUSG00000027257.
GeneIDi80708.
KEGGimmu:80708.
UCSCiuc008kvm.2. mouse.

Organism-specific databases

CTDi29763.
MGIiMGI:1891410. Pacsin3.

Phylogenomic databases

eggNOGiKOG2856. Eukaryota.
ENOG410XRX2. LUCA.
GeneTreeiENSGT00510000046376.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
InParanoidiQ99JB8.
KOiK20123.
OMAiAFESCQA.
OrthoDBiEOG091G0AS9.
PhylomeDBiQ99JB8.
TreeFamiTF313677.

Miscellaneous databases

PROiQ99JB8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027257.
ExpressionAtlasiQ99JB8. baseline and differential.
GenevisibleiQ99JB8. MM.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR028523. PACSIN3.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23065:SF18. PTHR23065:SF18. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPACN3_MOUSE
AccessioniPrimary (citable) accession number: Q99JB8
Secondary accession number(s): Q9EQP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.