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Q99JB8

- PACN3_MOUSE

UniProt

Q99JB8 - PACN3_MOUSE

Protein

Protein kinase C and casein kinase II substrate protein 3

Gene

Pacsin3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.4 Publications

    GO - Molecular functioni

    1. calcium channel inhibitor activity Source: UniProtKB
    2. cytoskeletal protein binding Source: MGI
    3. lipid binding Source: UniProtKB
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. endocytosis Source: UniProtKB-KW
    2. membrane tubulation Source: UniProtKB
    3. negative regulation of calcium ion transport Source: UniProtKB
    4. negative regulation of endocytosis Source: UniProtKB
    5. positive regulation of membrane protein ectodomain proteolysis Source: Ensembl

    Keywords - Biological processi

    Endocytosis

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein kinase C and casein kinase II substrate protein 3
    Gene namesi
    Name:Pacsin3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1891410. Pacsin3.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
    Note: Detected at the inner aspect of the plasma membrane in myotubes.

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi415 – 4151P → L: Loss of DNM1-, SYNJ1- and WASL-binding. Loss of effect on transferrin endocytosis. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 424424Protein kinase C and casein kinase II substrate protein 3PRO_0000161801Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei276 – 2761PhosphoserineBy similarity
    Modified residuei319 – 3191PhosphoserineBy similarity
    Modified residuei324 – 3241PhosphothreonineBy similarity
    Modified residuei327 – 3271PhosphoserineBy similarity
    Modified residuei354 – 3541Phosphoserine2 Publications
    Modified residuei383 – 3831PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ99JB8.
    PaxDbiQ99JB8.
    PRIDEiQ99JB8.

    PTM databases

    PhosphoSiteiQ99JB8.

    Expressioni

    Tissue specificityi

    Highly expressed in skeletal muscle, heart and lung; also detected in brain, kidney and uterus (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiQ99JB8.
    BgeeiQ99JB8.
    GenevestigatoriQ99JB8.

    Interactioni

    Subunit structurei

    Homodimer. May form heterooligomers with other PACSINs. Interacts (via SH3 domain) with DNM1, SYNJ1 and WASL. Interacts with TRPV4.4 Publications

    Protein-protein interaction databases

    IntActiQ99JB8. 4 interactions.
    MINTiMINT-1850676.

    Structurei

    Secondary structure

    1
    424
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni19 – 224
    Helixi23 – 7048
    Helixi75 – 10329
    Helixi105 – 11713
    Beta strandi122 – 1265
    Helixi127 – 17044
    Beta strandi186 – 1905
    Turni191 – 1933
    Helixi194 – 25360
    Helixi255 – 2573
    Helixi259 – 27416
    Helixi277 – 28812
    Beta strandi289 – 2924

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3M3WX-ray2.60A/B1-320[»]
    3QE6X-ray2.60A/B1-304[»]
    3SYVX-ray3.10A/B/C/D/E/F/G/H1-341[»]
    ProteinModelPortaliQ99JB8.
    SMRiQ99JB8. Positions 12-302, 324-420.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 7364FCHPROSITE-ProRule annotationAdd
    BLAST
    Domaini363 – 42462SH3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 304304F-BAR domainAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili23 – 2722501 PublicationAdd
    BLAST

    Domaini

    The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation.1 Publication

    Sequence similaritiesi

    Belongs to the PACSIN family.Curated
    Contains 1 FCH domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    eggNOGiNOG283356.
    GeneTreeiENSGT00510000046376.
    HOGENOMiHOG000007245.
    HOVERGENiHBG053486.
    InParanoidiQ99JB8.
    OMAiEQAFESC.
    PhylomeDBiQ99JB8.
    TreeFamiTF313677.

    Family and domain databases

    InterProiIPR001060. FCH_dom.
    IPR028523. PACSIN3.
    IPR001452. SH3_domain.
    [Graphical view]
    PANTHERiPTHR10959:SF1. PTHR10959:SF1. 1 hit.
    PfamiPF00611. FCH. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99JB8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPEEDAGGE VLGGSFWEAG NYRRTVQRVE DGHRLCGDLV SCFQERARIE    50
    KAYAQQLADW ARKWRGAVEK GPQYGTLEKA WHAFFTAAER LSELHLEVRE 100
    KLHGPDSERV RTWQRGAFHR PVLGGFRESR AAEDGFRKAQ KPWLKRLKEV 150
    EASKKSYHTA RKDEKTAQTR ESHAKADSSM SQEQLRKLQE RVGRCTKEAE 200
    KMKTQYEQTL AELNRYTPRY MEDMEQAFES CQAAERQRLL FFKDVLLTLH 250
    QHLDLSSSDK FHELHRDLQQ SIEAASDEED LRWWRSTHGP GMAMNWPQFE 300
    EWSLDTQRAI SRKEKGGRSP DEVTLTSIVP TRDGTAPPPQ SPSSPGSGQD 350
    EDWSDEESPR KVATGVRVRA LYDYAGQEAD ELSFRAGEEL LKMSEEDEQG 400
    WCQGQLQSGR IGLYPANYVE CVGA 424
    Length:424
    Mass (Da):48,585
    Last modified:June 1, 2001 - v1
    Checksum:i00475BC0321485B7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti360 – 3601R → G in AAG31022. (PubMed:11082044)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF149824 mRNA. Translation: AAG31022.1.
    AF242531 mRNA. Translation: AAK29208.1.
    BC003884 mRNA. Translation: AAH03884.1.
    CCDSiCCDS16429.1.
    RefSeqiNP_001276606.1. NM_001289677.1.
    NP_001276607.1. NM_001289678.1.
    NP_083009.1. NM_028733.4.
    NP_112019.2. NM_030880.3.
    XP_006500486.1. XM_006500423.1.
    XP_006500487.1. XM_006500424.1.
    XP_006500488.1. XM_006500425.1.
    XP_006500489.1. XM_006500426.1.
    XP_006500490.1. XM_006500427.1.
    XP_006500491.1. XM_006500428.1.
    UniGeneiMm.236650.

    Genome annotation databases

    EnsembliENSMUST00000028694; ENSMUSP00000028694; ENSMUSG00000027257.
    ENSMUST00000059566; ENSMUSP00000054391; ENSMUSG00000027257.
    ENSMUST00000111349; ENSMUSP00000106981; ENSMUSG00000027257.
    ENSMUST00000168916; ENSMUSP00000129175; ENSMUSG00000027257.
    GeneIDi80708.
    KEGGimmu:80708.
    UCSCiuc008kvn.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF149824 mRNA. Translation: AAG31022.1 .
    AF242531 mRNA. Translation: AAK29208.1 .
    BC003884 mRNA. Translation: AAH03884.1 .
    CCDSi CCDS16429.1.
    RefSeqi NP_001276606.1. NM_001289677.1.
    NP_001276607.1. NM_001289678.1.
    NP_083009.1. NM_028733.4.
    NP_112019.2. NM_030880.3.
    XP_006500486.1. XM_006500423.1.
    XP_006500487.1. XM_006500424.1.
    XP_006500488.1. XM_006500425.1.
    XP_006500489.1. XM_006500426.1.
    XP_006500490.1. XM_006500427.1.
    XP_006500491.1. XM_006500428.1.
    UniGenei Mm.236650.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3M3W X-ray 2.60 A/B 1-320 [» ]
    3QE6 X-ray 2.60 A/B 1-304 [» ]
    3SYV X-ray 3.10 A/B/C/D/E/F/G/H 1-341 [» ]
    ProteinModelPortali Q99JB8.
    SMRi Q99JB8. Positions 12-302, 324-420.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q99JB8. 4 interactions.
    MINTi MINT-1850676.

    PTM databases

    PhosphoSitei Q99JB8.

    Proteomic databases

    MaxQBi Q99JB8.
    PaxDbi Q99JB8.
    PRIDEi Q99JB8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028694 ; ENSMUSP00000028694 ; ENSMUSG00000027257 .
    ENSMUST00000059566 ; ENSMUSP00000054391 ; ENSMUSG00000027257 .
    ENSMUST00000111349 ; ENSMUSP00000106981 ; ENSMUSG00000027257 .
    ENSMUST00000168916 ; ENSMUSP00000129175 ; ENSMUSG00000027257 .
    GeneIDi 80708.
    KEGGi mmu:80708.
    UCSCi uc008kvn.2. mouse.

    Organism-specific databases

    CTDi 29763.
    MGIi MGI:1891410. Pacsin3.

    Phylogenomic databases

    eggNOGi NOG283356.
    GeneTreei ENSGT00510000046376.
    HOGENOMi HOG000007245.
    HOVERGENi HBG053486.
    InParanoidi Q99JB8.
    OMAi EQAFESC.
    PhylomeDBi Q99JB8.
    TreeFami TF313677.

    Miscellaneous databases

    NextBioi 350063.
    PROi Q99JB8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99JB8.
    Bgeei Q99JB8.
    Genevestigatori Q99JB8.

    Family and domain databases

    InterProi IPR001060. FCH_dom.
    IPR028523. PACSIN3.
    IPR001452. SH3_domain.
    [Graphical view ]
    PANTHERi PTHR10959:SF1. PTHR10959:SF1. 1 hit.
    Pfami PF00611. FCH. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "All three PACSIN isoforms bind to endocytic proteins and inhibit endocytosis."
      Modregger J., Ritter B., Witter B., Paulsson M., Plomann M.
      J. Cell Sci. 113:4511-4521(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH DNM1; SYNJ1 AND WASL, HOMOOLIGOMERIZATION, HETEROOLIGOMERIZATION WITH PACSIN1 AND PACSIN2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF PRO-415.
      Strain: C57BL/6J.
    2. "PACSIN 3 is a novel SH3 domain cytoplasmic adapter protein of the pacsin-syndapin-FAP52 gene family."
      Sumoy L., Pluvinet R., Andreu N., Estivill X., Escarceller M.
      Gene 262:199-205(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Neuron.
    4. "PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4."
      Cuajungco M.P., Grimm C., Oshima K., D'hoedt D., Nilius B., Mensenkamp A.R., Bindels R.J., Plomann M., Heller S.
      J. Biol. Chem. 281:18753-18762(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TRPV4.
    5. "PACSIN3 overexpression increases adipocyte glucose transport through GLUT1."
      Roach W., Plomann M.
      Biochem. Biophys. Res. Commun. 355:745-750(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "Stimulus-specific modulation of the cation channel TRPV4 by PACSIN 3."
      D'hoedt D., Owsianik G., Prenen J., Cuajungco M.P., Grimm C., Heller S., Voets T., Nilius B.
      J. Biol. Chem. 283:6272-6280(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TRPV4.
    8. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    9. "Rigidity of wedge loop in PACSIN 3 protein is a key factor in dictating diameters of tubules."
      Bai X., Meng G., Luo M., Zheng X.
      J. Biol. Chem. 287:22387-22396(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 1-320, COILED COIL, SUBUNIT, LIPID-BINDING, DOMAIN.

    Entry informationi

    Entry nameiPACN3_MOUSE
    AccessioniPrimary (citable) accession number: Q99JB8
    Secondary accession number(s): Q9EQP9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 2002
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3