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Q99JB7

- AMNLS_MOUSE

UniProt

Q99JB7 - AMNLS_MOUSE

Protein

Protein amnionless

Gene

Amn

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Necessary for efficient absorption of vitamin B12 By similarity. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm.By similarity3 Publications

    GO - Biological processi

    1. excretion Source: MGI
    2. multicellular organismal development Source: UniProtKB-KW
    3. protein localization Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Enzyme and pathway databases

    ReactomeiREACT_189089. Defective AMN causes hereditary megaloblastic anemia 1.
    REACT_189090. Defective CUBN causes hereditary megaloblastic anemia 1.
    REACT_189118. Cobalamin (Cbl, vitamin B12) transport and metabolism.
    REACT_213857. HDL-mediated lipid transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein amnionless
    Gene namesi
    Name:Amn
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1934943. Amn.

    Subcellular locationi

    GO - Cellular componenti

    1. apical part of cell Source: MGI
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 458439Protein amnionlessPRO_0000020703Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi27 – 271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi355 – 3551N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ99JB7.
    PaxDbiQ99JB7.
    PRIDEiQ99JB7.

    PTM databases

    PhosphoSiteiQ99JB7.

    Expressioni

    Tissue specificityi

    Expressed in polarized epithelial cells which are specialized in resorption or transport, specifically kidney proximal tubules and intestinal epithelium.1 Publication

    Developmental stagei

    Detected in primitive endoderm at E4.5, and on the apical surface of the visceral endoderm from E5.5 to E8.5. Expressed in mesonephric tubules at E11.5-12.5 and in the metanephric kidney beginning at E14.5. Expressed in the intestine from E16.5.1 Publication

    Gene expression databases

    BgeeiQ99JB7.
    CleanExiMM_AMN.
    GenevestigatoriQ99JB7.

    Interactioni

    Subunit structurei

    Interacts with CUBN/cubilin.1 Publication

    Protein-protein interaction databases

    IntActiQ99JB7. 3 interactions.
    MINTiMINT-4087626.
    STRINGi10090.ENSMUSP00000021707.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 362343ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini384 – 45875CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei363 – 38321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini203 – 25452VWFCAdd
    BLAST

    Sequence similaritiesi

    Contains 1 VWFC domain.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG82604.
    GeneTreeiENSGT00390000007463.
    HOGENOMiHOG000033922.
    HOVERGENiHBG037321.
    InParanoidiQ99JB7.
    KOiK18259.
    OMAiFDNPMFD.
    OrthoDBiEOG7ZGX2W.
    PhylomeDBiQ99JB7.
    TreeFamiTF323790.

    Family and domain databases

    InterProiIPR026112. AMN.
    [Graphical view]
    PANTHERiPTHR14995. PTHR14995. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99JB7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGALGRVLLW LQLCAMTRAA YKLWVPNTSF DTASNWNQNR TPCAGDAVQF    50
    PADKMVSVLV RDSHAISDML LPLDGELVLA SGAALSAAGG DSDPACNPGA 100
    PLLFRNPDRF SWLDPHLWSS GTQAPGLFSV DAERVPCSYD DVLFPRDGSF 150
    RVALGPGPNP VHVRSVSAVG QTFSRDEDLT AFLASREGRL RFHGSGALRV 200
    GSQACTDASG CVCGNAEMLP WICASLLQPL GGRCPQAACQ DPLLPQGQCC 250
    DLCGAIVSLT HDPTFDLERY RARLLDLFLK QPQYQGLQVA VSKVLRDAHT 300
    EIQVVLVETE HATGAAGQLG HALLQDAVAQ GSVLGIVSAT LRQSGKPMTA 350
    DSELNQSSSG AGLAGGVAAL VLLALLGTVL LLLHRSGRLR WRRHEDAEPV 400
    SAGLPLGFRN PIFDAIVFKQ QPSVELPDSA QKVDILDIDT KFGCFVNPLF 450
    AGEAEAEA 458
    Length:458
    Mass (Da):48,696
    Last modified:June 1, 2001 - v1
    Checksum:i00789EE67C35443F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF320615 Genomic DNA. Translation: AAK37476.1.
    AF320616 Genomic DNA. Translation: AAK37477.1.
    AF320619 mRNA. Translation: AAK37478.1.
    BC087954 mRNA. Translation: AAH87954.1.
    CCDSiCCDS36564.1.
    RefSeqiNP_291081.2. NM_033603.2.
    UniGeneiMm.197639.

    Genome annotation databases

    EnsembliENSMUST00000021707; ENSMUSP00000021707; ENSMUSG00000021278.
    GeneIDi93835.
    KEGGimmu:93835.
    UCSCiuc007pcp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF320615 Genomic DNA. Translation: AAK37476.1 .
    AF320616 Genomic DNA. Translation: AAK37477.1 .
    AF320619 mRNA. Translation: AAK37478.1 .
    BC087954 mRNA. Translation: AAH87954.1 .
    CCDSi CCDS36564.1.
    RefSeqi NP_291081.2. NM_033603.2.
    UniGenei Mm.197639.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q99JB7. 3 interactions.
    MINTi MINT-4087626.
    STRINGi 10090.ENSMUSP00000021707.

    PTM databases

    PhosphoSitei Q99JB7.

    Proteomic databases

    MaxQBi Q99JB7.
    PaxDbi Q99JB7.
    PRIDEi Q99JB7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021707 ; ENSMUSP00000021707 ; ENSMUSG00000021278 .
    GeneIDi 93835.
    KEGGi mmu:93835.
    UCSCi uc007pcp.1. mouse.

    Organism-specific databases

    CTDi 81693.
    MGIi MGI:1934943. Amn.

    Phylogenomic databases

    eggNOGi NOG82604.
    GeneTreei ENSGT00390000007463.
    HOGENOMi HOG000033922.
    HOVERGENi HBG037321.
    InParanoidi Q99JB7.
    KOi K18259.
    OMAi FDNPMFD.
    OrthoDBi EOG7ZGX2W.
    PhylomeDBi Q99JB7.
    TreeFami TF323790.

    Enzyme and pathway databases

    Reactomei REACT_189089. Defective AMN causes hereditary megaloblastic anemia 1.
    REACT_189090. Defective CUBN causes hereditary megaloblastic anemia 1.
    REACT_189118. Cobalamin (Cbl, vitamin B12) transport and metabolism.
    REACT_213857. HDL-mediated lipid transport.

    Miscellaneous databases

    NextBioi 351681.
    PROi Q99JB7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q99JB7.
    CleanExi MM_AMN.
    Genevestigatori Q99JB7.

    Family and domain databases

    InterProi IPR026112. AMN.
    [Graphical view ]
    PANTHERi PTHR14995. PTHR14995. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The amnionless gene, essential for mouse gastrulation, encodes a visceral-endoderm-specific protein with an extracellular cysteine-rich domain."
      Kalantry S., Manning S., Haub O., Tomihara-Newberger C., Lee H.-G., Fangman J., Disteche C.M., Manova K., Lacy E.
      Nat. Genet. 27:412-416(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DEVELOPMENTAL STAGE.
      Strain: 129/SvJ and RIII.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    3. "The amn gene product is required in extraembryonic tissues for the generation of middle primitive streak derivatives."
      Tomihara-Newberger C., Haub O., Lee H.-G., Soares V., Manova K., Lacy E.
      Dev. Biol. 204:34-54(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Mouse amnionless, which is required for primitive streak assembly, mediates cell-surface localization and endocytic function of cubilin on visceral endoderm and kidney proximal tubules."
      Strope S., Rivi R., Metzger T., Manova K., Lacy E.
      Development 131:4787-4795(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CUBN, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiAMNLS_MOUSE
    AccessioniPrimary (citable) accession number: Q99JB7
    Secondary accession number(s): Q5I0U1, Q99MP9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2003
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3