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Protein

Protein amnionless

Gene

Amn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for efficient absorption of vitamin B12 (By similarity). Required for normal CUBN-mediated protein transport in the kidney (PubMed:15342463). May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm.By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

  • cobalamin transport Source: MGI
  • excretion Source: MGI
  • Golgi to plasma membrane protein transport Source: MGI
  • multicellular organism development Source: UniProtKB-KW
  • protein localization Source: MGI
  • receptor-mediated endocytosis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-194223. HDL-mediated lipid transport.
R-MMU-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein amnionless
Gene namesi
Name:Amn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1934943. Amn.

Subcellular locationi

  • Apical cell membrane 1 Publication; Single-pass type I membrane protein Curated
  • Cell membrane By similarity; Peripheral membrane protein By similarity
  • Endosome By similarity
  • Membranecoated pit By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 362ExtracellularSequence analysisAdd BLAST343
Transmembranei363 – 383HelicalSequence analysisAdd BLAST21
Topological domaini384 – 458CytoplasmicSequence analysisAdd BLAST75

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Coated pit, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002070320 – 458Protein amnionlessAdd BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...)Sequence analysis1
Glycosylationi355N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ99JB7.
PaxDbiQ99JB7.
PeptideAtlasiQ99JB7.
PRIDEiQ99JB7.

PTM databases

iPTMnetiQ99JB7.
PhosphoSitePlusiQ99JB7.

Expressioni

Tissue specificityi

Expressed in polarized epithelial cells which are specialized in resorption or transport, specifically kidney proximal tubules and intestinal epithelium.1 Publication

Developmental stagei

Detected in primitive endoderm at E4.5, and on the apical surface of the visceral endoderm from E5.5 to E8.5. Expressed in mesonephric tubules at E11.5-12.5 and in the metanephric kidney beginning at E14.5. Expressed in the intestine from E16.5.1 Publication

Gene expression databases

BgeeiENSMUSG00000021278.
CleanExiMM_AMN.
GenevisibleiQ99JB7. MM.

Interactioni

Subunit structurei

Interacts with CUBN/cubilin.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ99JB7. 3 interactors.
MINTiMINT-4087626.
STRINGi10090.ENSMUSP00000021707.

Structurei

3D structure databases

ProteinModelPortaliQ99JB7.
SMRiQ99JB7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini203 – 254VWFCAdd BLAST52

Sequence similaritiesi

Contains 1 VWFC domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II2Q. Eukaryota.
ENOG410XS7P. LUCA.
GeneTreeiENSGT00390000007463.
HOGENOMiHOG000033922.
HOVERGENiHBG037321.
InParanoidiQ99JB7.
KOiK18259.
OMAiEAQPWIC.
OrthoDBiEOG091G11HV.
PhylomeDBiQ99JB7.
TreeFamiTF323790.

Family and domain databases

InterProiIPR026112. AMN.
[Graphical view]
PANTHERiPTHR14995. PTHR14995. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99JB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGALGRVLLW LQLCAMTRAA YKLWVPNTSF DTASNWNQNR TPCAGDAVQF
60 70 80 90 100
PADKMVSVLV RDSHAISDML LPLDGELVLA SGAALSAAGG DSDPACNPGA
110 120 130 140 150
PLLFRNPDRF SWLDPHLWSS GTQAPGLFSV DAERVPCSYD DVLFPRDGSF
160 170 180 190 200
RVALGPGPNP VHVRSVSAVG QTFSRDEDLT AFLASREGRL RFHGSGALRV
210 220 230 240 250
GSQACTDASG CVCGNAEMLP WICASLLQPL GGRCPQAACQ DPLLPQGQCC
260 270 280 290 300
DLCGAIVSLT HDPTFDLERY RARLLDLFLK QPQYQGLQVA VSKVLRDAHT
310 320 330 340 350
EIQVVLVETE HATGAAGQLG HALLQDAVAQ GSVLGIVSAT LRQSGKPMTA
360 370 380 390 400
DSELNQSSSG AGLAGGVAAL VLLALLGTVL LLLHRSGRLR WRRHEDAEPV
410 420 430 440 450
SAGLPLGFRN PIFDAIVFKQ QPSVELPDSA QKVDILDIDT KFGCFVNPLF

AGEAEAEA
Length:458
Mass (Da):48,696
Last modified:June 1, 2001 - v1
Checksum:i00789EE67C35443F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320615 Genomic DNA. Translation: AAK37476.1.
AF320616 Genomic DNA. Translation: AAK37477.1.
AF320619 mRNA. Translation: AAK37478.1.
BC087954 mRNA. Translation: AAH87954.1.
CCDSiCCDS36564.1.
RefSeqiNP_291081.2. NM_033603.3.
UniGeneiMm.197639.

Genome annotation databases

EnsembliENSMUST00000021707; ENSMUSP00000021707; ENSMUSG00000021278.
GeneIDi93835.
KEGGimmu:93835.
UCSCiuc007pcp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF320615 Genomic DNA. Translation: AAK37476.1.
AF320616 Genomic DNA. Translation: AAK37477.1.
AF320619 mRNA. Translation: AAK37478.1.
BC087954 mRNA. Translation: AAH87954.1.
CCDSiCCDS36564.1.
RefSeqiNP_291081.2. NM_033603.3.
UniGeneiMm.197639.

3D structure databases

ProteinModelPortaliQ99JB7.
SMRiQ99JB7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99JB7. 3 interactors.
MINTiMINT-4087626.
STRINGi10090.ENSMUSP00000021707.

PTM databases

iPTMnetiQ99JB7.
PhosphoSitePlusiQ99JB7.

Proteomic databases

MaxQBiQ99JB7.
PaxDbiQ99JB7.
PeptideAtlasiQ99JB7.
PRIDEiQ99JB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021707; ENSMUSP00000021707; ENSMUSG00000021278.
GeneIDi93835.
KEGGimmu:93835.
UCSCiuc007pcp.1. mouse.

Organism-specific databases

CTDi81693.
MGIiMGI:1934943. Amn.

Phylogenomic databases

eggNOGiENOG410II2Q. Eukaryota.
ENOG410XS7P. LUCA.
GeneTreeiENSGT00390000007463.
HOGENOMiHOG000033922.
HOVERGENiHBG037321.
InParanoidiQ99JB7.
KOiK18259.
OMAiEAQPWIC.
OrthoDBiEOG091G11HV.
PhylomeDBiQ99JB7.
TreeFamiTF323790.

Enzyme and pathway databases

ReactomeiR-MMU-194223. HDL-mediated lipid transport.
R-MMU-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Miscellaneous databases

PROiQ99JB7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021278.
CleanExiMM_AMN.
GenevisibleiQ99JB7. MM.

Family and domain databases

InterProiIPR026112. AMN.
[Graphical view]
PANTHERiPTHR14995. PTHR14995. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMNLS_MOUSE
AccessioniPrimary (citable) accession number: Q99JB7
Secondary accession number(s): Q5I0U1, Q99MP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.