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Q99JB7

- AMNLS_MOUSE

UniProt

Q99JB7 - AMNLS_MOUSE

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Protein

Protein amnionless

Gene

Amn

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Necessary for efficient absorption of vitamin B12 (By similarity). May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm.By similarity3 Publications

GO - Biological processi

  1. cobalamin transport Source: Ensembl
  2. excretion Source: MGI
  3. Golgi to plasma membrane protein transport Source: Ensembl
  4. multicellular organismal development Source: UniProtKB-KW
  5. protein localization Source: MGI
  6. receptor-mediated endocytosis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Enzyme and pathway databases

ReactomeiREACT_189089. Defective AMN causes hereditary megaloblastic anemia 1.
REACT_189090. Defective CUBN causes hereditary megaloblastic anemia 1.
REACT_189118. Cobalamin (Cbl, vitamin B12) transport and metabolism.
REACT_213857. HDL-mediated lipid transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein amnionless
Gene namesi
Name:Amn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1934943. Amn.

Subcellular locationi

GO - Cellular componenti

  1. apical part of cell Source: MGI
  2. apical plasma membrane Source: Ensembl
  3. endocytic vesicle Source: Ensembl
  4. extracellular space Source: Ensembl
  5. extracellular vesicular exosome Source: Ensembl
  6. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 458439Protein amnionlessPRO_0000020703Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi27 – 271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi355 – 3551N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ99JB7.
PaxDbiQ99JB7.
PRIDEiQ99JB7.

PTM databases

PhosphoSiteiQ99JB7.

Expressioni

Tissue specificityi

Expressed in polarized epithelial cells which are specialized in resorption or transport, specifically kidney proximal tubules and intestinal epithelium.1 Publication

Developmental stagei

Detected in primitive endoderm at E4.5, and on the apical surface of the visceral endoderm from E5.5 to E8.5. Expressed in mesonephric tubules at E11.5-12.5 and in the metanephric kidney beginning at E14.5. Expressed in the intestine from E16.5.1 Publication

Gene expression databases

BgeeiQ99JB7.
CleanExiMM_AMN.
GenevestigatoriQ99JB7.

Interactioni

Subunit structurei

Interacts with CUBN/cubilin.1 Publication

Protein-protein interaction databases

IntActiQ99JB7. 3 interactions.
MINTiMINT-4087626.
STRINGi10090.ENSMUSP00000021707.

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 362343ExtracellularSequence AnalysisAdd
BLAST
Topological domaini384 – 45875CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei363 – 38321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini203 – 25452VWFCAdd
BLAST

Sequence similaritiesi

Contains 1 VWFC domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG82604.
GeneTreeiENSGT00390000007463.
HOGENOMiHOG000033922.
HOVERGENiHBG037321.
InParanoidiQ99JB7.
KOiK18259.
OMAiFDNPMFD.
OrthoDBiEOG7ZGX2W.
PhylomeDBiQ99JB7.
TreeFamiTF323790.

Family and domain databases

InterProiIPR026112. AMN.
[Graphical view]
PANTHERiPTHR14995. PTHR14995. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99JB7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGALGRVLLW LQLCAMTRAA YKLWVPNTSF DTASNWNQNR TPCAGDAVQF
60 70 80 90 100
PADKMVSVLV RDSHAISDML LPLDGELVLA SGAALSAAGG DSDPACNPGA
110 120 130 140 150
PLLFRNPDRF SWLDPHLWSS GTQAPGLFSV DAERVPCSYD DVLFPRDGSF
160 170 180 190 200
RVALGPGPNP VHVRSVSAVG QTFSRDEDLT AFLASREGRL RFHGSGALRV
210 220 230 240 250
GSQACTDASG CVCGNAEMLP WICASLLQPL GGRCPQAACQ DPLLPQGQCC
260 270 280 290 300
DLCGAIVSLT HDPTFDLERY RARLLDLFLK QPQYQGLQVA VSKVLRDAHT
310 320 330 340 350
EIQVVLVETE HATGAAGQLG HALLQDAVAQ GSVLGIVSAT LRQSGKPMTA
360 370 380 390 400
DSELNQSSSG AGLAGGVAAL VLLALLGTVL LLLHRSGRLR WRRHEDAEPV
410 420 430 440 450
SAGLPLGFRN PIFDAIVFKQ QPSVELPDSA QKVDILDIDT KFGCFVNPLF

AGEAEAEA
Length:458
Mass (Da):48,696
Last modified:June 1, 2001 - v1
Checksum:i00789EE67C35443F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF320615 Genomic DNA. Translation: AAK37476.1.
AF320616 Genomic DNA. Translation: AAK37477.1.
AF320619 mRNA. Translation: AAK37478.1.
BC087954 mRNA. Translation: AAH87954.1.
CCDSiCCDS36564.1.
RefSeqiNP_291081.2. NM_033603.2.
UniGeneiMm.197639.

Genome annotation databases

EnsembliENSMUST00000021707; ENSMUSP00000021707; ENSMUSG00000021278.
GeneIDi93835.
KEGGimmu:93835.
UCSCiuc007pcp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF320615 Genomic DNA. Translation: AAK37476.1 .
AF320616 Genomic DNA. Translation: AAK37477.1 .
AF320619 mRNA. Translation: AAK37478.1 .
BC087954 mRNA. Translation: AAH87954.1 .
CCDSi CCDS36564.1.
RefSeqi NP_291081.2. NM_033603.2.
UniGenei Mm.197639.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q99JB7. 3 interactions.
MINTi MINT-4087626.
STRINGi 10090.ENSMUSP00000021707.

PTM databases

PhosphoSitei Q99JB7.

Proteomic databases

MaxQBi Q99JB7.
PaxDbi Q99JB7.
PRIDEi Q99JB7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021707 ; ENSMUSP00000021707 ; ENSMUSG00000021278 .
GeneIDi 93835.
KEGGi mmu:93835.
UCSCi uc007pcp.1. mouse.

Organism-specific databases

CTDi 81693.
MGIi MGI:1934943. Amn.

Phylogenomic databases

eggNOGi NOG82604.
GeneTreei ENSGT00390000007463.
HOGENOMi HOG000033922.
HOVERGENi HBG037321.
InParanoidi Q99JB7.
KOi K18259.
OMAi FDNPMFD.
OrthoDBi EOG7ZGX2W.
PhylomeDBi Q99JB7.
TreeFami TF323790.

Enzyme and pathway databases

Reactomei REACT_189089. Defective AMN causes hereditary megaloblastic anemia 1.
REACT_189090. Defective CUBN causes hereditary megaloblastic anemia 1.
REACT_189118. Cobalamin (Cbl, vitamin B12) transport and metabolism.
REACT_213857. HDL-mediated lipid transport.

Miscellaneous databases

NextBioi 351681.
PROi Q99JB7.
SOURCEi Search...

Gene expression databases

Bgeei Q99JB7.
CleanExi MM_AMN.
Genevestigatori Q99JB7.

Family and domain databases

InterProi IPR026112. AMN.
[Graphical view ]
PANTHERi PTHR14995. PTHR14995. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The amnionless gene, essential for mouse gastrulation, encodes a visceral-endoderm-specific protein with an extracellular cysteine-rich domain."
    Kalantry S., Manning S., Haub O., Tomihara-Newberger C., Lee H.-G., Fangman J., Disteche C.M., Manova K., Lacy E.
    Nat. Genet. 27:412-416(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DEVELOPMENTAL STAGE.
    Strain: 129/SvJ and RIII.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. "The amn gene product is required in extraembryonic tissues for the generation of middle primitive streak derivatives."
    Tomihara-Newberger C., Haub O., Lee H.-G., Soares V., Manova K., Lacy E.
    Dev. Biol. 204:34-54(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Mouse amnionless, which is required for primitive streak assembly, mediates cell-surface localization and endocytic function of cubilin on visceral endoderm and kidney proximal tubules."
    Strope S., Rivi R., Metzger T., Manova K., Lacy E.
    Development 131:4787-4795(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CUBN, TISSUE SPECIFICITY.

Entry informationi

Entry nameiAMNLS_MOUSE
AccessioniPrimary (citable) accession number: Q99JB7
Secondary accession number(s): Q5I0U1, Q99MP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3