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Q99JB7 (AMNLS_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein amnionless
Gene names
Name:Amn
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length458 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for efficient absorption of vitamin B12 By similarity. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm. Ref.1 Ref.3 Ref.4

Subunit structure

Interacts with CUBN/cubilin. Ref.4

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

Expressed in polarized epithelial cells which are specialized in resorption or transport, specifically kidney proximal tubules and intestinal epithelium. Ref.4

Developmental stage

Detected in primitive endoderm at E4.5, and on the apical surface of the visceral endoderm from E5.5 to E8.5. Expressed in mesonephric tubules at E11.5-12.5 and in the metanephric kidney beginning at E14.5. Expressed in the intestine from E16.5. Ref.1

Sequence similarities

Contains 1 VWFC domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 458439Protein amnionless
PRO_0000020703

Regions

Topological domain20 – 362343Extracellular Potential
Transmembrane363 – 38321Helical; Potential
Topological domain384 – 45875Cytoplasmic Potential
Domain203 – 25452VWFC

Amino acid modifications

Glycosylation271N-linked (GlcNAc...) Potential
Glycosylation3551N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q99JB7 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 00789EE67C35443F

FASTA45848,696
        10         20         30         40         50         60 
MGALGRVLLW LQLCAMTRAA YKLWVPNTSF DTASNWNQNR TPCAGDAVQF PADKMVSVLV 

        70         80         90        100        110        120 
RDSHAISDML LPLDGELVLA SGAALSAAGG DSDPACNPGA PLLFRNPDRF SWLDPHLWSS 

       130        140        150        160        170        180 
GTQAPGLFSV DAERVPCSYD DVLFPRDGSF RVALGPGPNP VHVRSVSAVG QTFSRDEDLT 

       190        200        210        220        230        240 
AFLASREGRL RFHGSGALRV GSQACTDASG CVCGNAEMLP WICASLLQPL GGRCPQAACQ 

       250        260        270        280        290        300 
DPLLPQGQCC DLCGAIVSLT HDPTFDLERY RARLLDLFLK QPQYQGLQVA VSKVLRDAHT 

       310        320        330        340        350        360 
EIQVVLVETE HATGAAGQLG HALLQDAVAQ GSVLGIVSAT LRQSGKPMTA DSELNQSSSG 

       370        380        390        400        410        420 
AGLAGGVAAL VLLALLGTVL LLLHRSGRLR WRRHEDAEPV SAGLPLGFRN PIFDAIVFKQ 

       430        440        450 
QPSVELPDSA QKVDILDIDT KFGCFVNPLF AGEAEAEA 

« Hide

References

« Hide 'large scale' references
[1]"The amnionless gene, essential for mouse gastrulation, encodes a visceral-endoderm-specific protein with an extracellular cysteine-rich domain."
Kalantry S., Manning S., Haub O., Tomihara-Newberger C., Lee H.-G., Fangman J., Disteche C.M., Manova K., Lacy E.
Nat. Genet. 27:412-416(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DEVELOPMENTAL STAGE.
Strain: 129/SvJ and RIII.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[3]"The amn gene product is required in extraembryonic tissues for the generation of middle primitive streak derivatives."
Tomihara-Newberger C., Haub O., Lee H.-G., Soares V., Manova K., Lacy E.
Dev. Biol. 204:34-54(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Mouse amnionless, which is required for primitive streak assembly, mediates cell-surface localization and endocytic function of cubilin on visceral endoderm and kidney proximal tubules."
Strope S., Rivi R., Metzger T., Manova K., Lacy E.
Development 131:4787-4795(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CUBN, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF320615 Genomic DNA. Translation: AAK37476.1.
AF320616 Genomic DNA. Translation: AAK37477.1.
AF320619 mRNA. Translation: AAK37478.1.
BC087954 mRNA. Translation: AAH87954.1.
CCDSCCDS36564.1.
RefSeqNP_291081.2. NM_033603.2.
UniGeneMm.197639.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ99JB7. 3 interactions.
MINTMINT-4087626.
STRING10090.ENSMUSP00000021707.

PTM databases

PhosphoSiteQ99JB7.

Proteomic databases

MaxQBQ99JB7.
PaxDbQ99JB7.
PRIDEQ99JB7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021707; ENSMUSP00000021707; ENSMUSG00000021278.
GeneID93835.
KEGGmmu:93835.
UCSCuc007pcp.1. mouse.

Organism-specific databases

CTD81693.
MGIMGI:1934943. Amn.

Phylogenomic databases

eggNOGNOG82604.
GeneTreeENSGT00390000007463.
HOGENOMHOG000033922.
HOVERGENHBG037321.
InParanoidQ99JB7.
KOK18259.
OMAFDNPMFD.
OrthoDBEOG7ZGX2W.
PhylomeDBQ99JB7.
TreeFamTF323790.

Gene expression databases

BgeeQ99JB7.
CleanExMM_AMN.
GenevestigatorQ99JB7.

Family and domain databases

InterProIPR026112. AMN.
[Graphical view]
PANTHERPTHR14995. PTHR14995. 1 hit.
ProtoNetSearch...

Other

NextBio351681.
PROQ99JB7.
SOURCESearch...

Entry information

Entry nameAMNLS_MOUSE
AccessionPrimary (citable) accession number: Q99JB7
Secondary accession number(s): Q5I0U1, Q99MP9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot