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Q99JA4 (CLTR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteinyl leukotriene receptor 1

Short name=CysLTR1
Alternative name(s):
Cysteinyl leukotriene D4 receptor
Short name=LTD4 receptor
Gene names
Name:Cysltr1
Synonyms:Cyslt1, Cyslt1r
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for cysteinyl leukotrienes mediating constriction of the microvascular smooth muscle during an inflammatory response. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Widely expressed, with higher expression in the lung and skin, intermediate levels in the heart, kidney and stomach and lower levels in several other tissues. Isoform 1 is the most abundant form in all tested tissues.

Miscellaneous

MK-571, a selective antagonist, was shown to inhibit eosinophilia, bronchial hyperreactivity and microvascular leakage. Zafirlukast (Accolate) and pranlukast (Onon) were also shown to be selective antagonists.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99JA4-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99JA4-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 352352Cysteinyl leukotriene receptor 1
PRO_0000069300

Regions

Topological domain1 – 4343Extracellular Potential
Transmembrane44 – 6421Helical; Name=1; Potential
Topological domain65 – 728Cytoplasmic Potential
Transmembrane73 – 9321Helical; Name=2; Potential
Topological domain94 – 12128Extracellular Potential
Transmembrane122 – 14221Helical; Name=3; Potential
Topological domain143 – 15614Cytoplasmic Potential
Transmembrane157 – 17721Helical; Name=4; Potential
Topological domain178 – 20831Extracellular Potential
Transmembrane209 – 22921Helical; Name=5; Potential
Topological domain230 – 24516Cytoplasmic Potential
Transmembrane246 – 26621Helical; Name=6; Potential
Topological domain267 – 29125Extracellular Potential
Transmembrane292 – 31221Helical; Name=7; Potential
Topological domain313 – 35240Cytoplasmic Potential

Amino acid modifications

Glycosylation151N-linked (GlcNAc...) Potential
Glycosylation191N-linked (GlcNAc...) Potential
Glycosylation261N-linked (GlcNAc...) Potential
Glycosylation1841N-linked (GlcNAc...) Potential
Disulfide bond111 ↔ 188 By similarity

Natural variations

Alternative sequence1 – 1313Missing in isoform 2.
VSP_001921

Experimental info

Sequence conflict1761Y → D in AAF73047. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 5BDC94B3F1CD0CAB

FASTA35240,715
        10         20         30         40         50         60 
MYLQGTKQTF LENMNGTENL TTSLINNTCH DTIDEFRNQV YSTMYSVISV VGFFGNSFVL 

        70         80         90        100        110        120 
YVLIKTYHEK SAFQVYMINL AIADLLCVCT LPLRVVYYVH KGKWLFGDFL CRLTTYALYV 

       130        140        150        160        170        180 
NLYCSIFFMT AMSFFRCVAI VFPVQNINLV TQKKARFVCI GIWIFVILTS SPFLMYKSYQ 

       190        200        210        220        230        240 
DEKNNTKCFE PPQNNQAKKY VLILHYVSLF FGFIIPFVTI IVCYTMIILT LLKNTMKKNM 

       250        260        270        280        290        300 
PSRRKAIGMI IVVTAAFLVS FMPYHIQRTI HLHLLHSETR PCDSVLRMQK SVVITLSLAA 

       310        320        330        340        350 
SNCCFDPLLY FFSGGNFRRR LSTFRKHSLS SMTYVPKKKA SLPEKGEEIC NE 

« Hide

Isoform 2 (Short) [UniParc].

Checksum: 27BF72C0F3F0F00F
Show »

FASTA33939,160

References

« Hide 'large scale' references
[1]"Identification in mice of two isoforms of the cysteinyl leukotriene 1 receptor that result from alternative splicing."
Maekawa A., Kanaoka Y., Lam B.K., Austen K.F.
Proc. Natl. Acad. Sci. U.S.A. 98:2256-2261(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
Strain: 129/Sv.
[2]"Molecular cloning and functional characterization of murine cysteinyl-leukotriene 1 (CysLT1) receptors."
Martin V., Sawyer N., Stocco R., Unett D., Lerner M.R., Abramovitz M., Funk C.D.
Biochem. Pharmacol. 62:1193-1200(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Trachea.
[3]"Identification of a murine cysteinyl leukotriene receptor by expression in Xenopus laevis oocytes."
Mollerup J., Jorgensen S.T., Hougaard C., Hoffmann E.K.
Biochim. Biophys. Acta 1517:455-459(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Blood.
[4]Ogasawara H., Izumi T., Shimizu T.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: T-cell.
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Colon.
[6]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Czech II.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF329272 Genomic DNA. Translation: AAK16715.1.
AF329272 Genomic DNA. Translation: AAK16716.1.
AF205830 mRNA. Translation: AAK15433.1.
AF263370 mRNA. Translation: AAF73047.1.
AB044087 mRNA. Translation: BAA96809.1.
AK033476 mRNA. Translation: BAC28308.1.
AL732460 Genomic DNA. Translation: CAM13712.1.
BC027102 mRNA. Translation: AAH27102.1.
CCDSCCDS41098.1. [Q99JA4-1]
RefSeqNP_001268788.1. NM_001281859.1. [Q99JA4-1]
NP_001268791.1. NM_001281862.1. [Q99JA4-2]
NP_067451.2. NM_021476.5. [Q99JA4-1]
XP_006528275.1. XM_006528212.1. [Q99JA4-2]
UniGeneMm.287166.

3D structure databases

ProteinModelPortalQ99JA4.
SMRQ99JA4. Positions 40-331.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48916N.

Chemistry

GuidetoPHARMACOLOGY269.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ99JA4.

Proteomic databases

PaxDbQ99JA4.
PRIDEQ99JA4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000064892; ENSMUSP00000063520; ENSMUSG00000052821. [Q99JA4-1]
ENSMUST00000113480; ENSMUSP00000109108; ENSMUSG00000052821. [Q99JA4-1]
GeneID58861.
KEGGmmu:58861.
UCSCuc009ubt.1. mouse. [Q99JA4-1]

Organism-specific databases

CTD10800.
MGIMGI:1926218. Cysltr1.

Phylogenomic databases

eggNOGNOG119185.
GeneTreeENSGT00750000117402.
HOGENOMHOG000236328.
HOVERGENHBG099611.
InParanoidQ99JA4.
KOK04322.
OMAPQDNQAK.
OrthoDBEOG7992R9.
PhylomeDBQ99JA4.
TreeFamTF350009.

Gene expression databases

BgeeQ99JA4.
CleanExMM_CYSLTR1.
GenevestigatorQ99JA4.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR013310. CLT1_recept.
IPR004071. Cyst_leuk_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01902. CYSLT1RECPTR.
PR01533. CYSLTRECPTR.
PR00237. GPCRRHODOPSN.
PROSITEPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio314416.
PROQ99JA4.
SOURCESearch...

Entry information

Entry nameCLTR1_MOUSE
AccessionPrimary (citable) accession number: Q99JA4
Secondary accession number(s): Q544P1, Q9JJ71, Q9JK47
Entry history
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries