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Protein

Cysteinyl leukotriene receptor 1

Gene

Cysltr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for cysteinyl leukotrienes mediating constriction of the microvascular smooth muscle during an inflammatory response. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.

GO - Molecular functioni

  1. leukotriene receptor activity Source: MGI

GO - Biological processi

  1. calcium ion transport Source: MGI
  2. cell surface receptor signaling pathway Source: MGI
  3. chemotaxis Source: MGI
  4. inflammatory response Source: Ensembl
  5. positive regulation of angiogenesis Source: Ensembl
  6. positive regulation of vasoconstriction Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_230951. Leukotriene receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteinyl leukotriene receptor 1
Short name:
CysLTR1
Alternative name(s):
Cysteinyl leukotriene D4 receptor
Short name:
LTD4 receptor
Gene namesi
Name:Cysltr1
Synonyms:Cyslt1, Cyslt1r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1926218. Cysltr1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4343ExtracellularSequence AnalysisAdd
BLAST
Transmembranei44 – 6421Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini65 – 728CytoplasmicSequence Analysis
Transmembranei73 – 9321Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini94 – 12128ExtracellularSequence AnalysisAdd
BLAST
Transmembranei122 – 14221Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini143 – 15614CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei157 – 17721Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini178 – 20831ExtracellularSequence AnalysisAdd
BLAST
Transmembranei209 – 22921Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini230 – 24516CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei246 – 26621Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini267 – 29125ExtracellularSequence AnalysisAdd
BLAST
Transmembranei292 – 31221Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini313 – 35240CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Cysteinyl leukotriene receptor 1PRO_0000069300Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi15 – 151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi19 – 191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi26 – 261N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi111 ↔ 188PROSITE-ProRule annotation
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ99JA4.
PRIDEiQ99JA4.

PTM databases

PhosphoSiteiQ99JA4.

Expressioni

Tissue specificityi

Widely expressed, with higher expression in the lung and skin, intermediate levels in the heart, kidney and stomach and lower levels in several other tissues. Isoform 1 is the most abundant form in all tested tissues.

Gene expression databases

BgeeiQ99JA4.
CleanExiMM_CYSLTR1.
GenevestigatoriQ99JA4.

Interactioni

Protein-protein interaction databases

DIPiDIP-48916N.

Structurei

3D structure databases

ProteinModelPortaliQ99JA4.
SMRiQ99JA4. Positions 40-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG119185.
GeneTreeiENSGT00760000118784.
HOGENOMiHOG000236328.
HOVERGENiHBG099611.
InParanoidiQ99JA4.
KOiK04322.
OMAiPQDNQAK.
OrthoDBiEOG7992R9.
PhylomeDBiQ99JA4.
TreeFamiTF350009.

Family and domain databases

InterProiIPR013310. CLT1_recept.
IPR004071. Cyst_leuk_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01902. CYSLT1RECPTR.
PR01533. CYSLTRECPTR.
PR00237. GPCRRHODOPSN.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99JA4-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYLQGTKQTF LENMNGTENL TTSLINNTCH DTIDEFRNQV YSTMYSVISV
60 70 80 90 100
VGFFGNSFVL YVLIKTYHEK SAFQVYMINL AIADLLCVCT LPLRVVYYVH
110 120 130 140 150
KGKWLFGDFL CRLTTYALYV NLYCSIFFMT AMSFFRCVAI VFPVQNINLV
160 170 180 190 200
TQKKARFVCI GIWIFVILTS SPFLMYKSYQ DEKNNTKCFE PPQNNQAKKY
210 220 230 240 250
VLILHYVSLF FGFIIPFVTI IVCYTMIILT LLKNTMKKNM PSRRKAIGMI
260 270 280 290 300
IVVTAAFLVS FMPYHIQRTI HLHLLHSETR PCDSVLRMQK SVVITLSLAA
310 320 330 340 350
SNCCFDPLLY FFSGGNFRRR LSTFRKHSLS SMTYVPKKKA SLPEKGEEIC

NE
Length:352
Mass (Da):40,715
Last modified:June 1, 2001 - v1
Checksum:i5BDC94B3F1CD0CAB
GO
Isoform 2 (identifier: Q99JA4-2) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Show »
Length:339
Mass (Da):39,160
Checksum:i27BF72C0F3F0F00F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti176 – 1761Y → D in AAF73047 (PubMed:11342226).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1313Missing in isoform 2. 1 PublicationVSP_001921Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF329272 Genomic DNA. Translation: AAK16715.1.
AF329272 Genomic DNA. Translation: AAK16716.1.
AF205830 mRNA. Translation: AAK15433.1.
AF263370 mRNA. Translation: AAF73047.1.
AB044087 mRNA. Translation: BAA96809.1.
AK033476 mRNA. Translation: BAC28308.1.
AL732460 Genomic DNA. Translation: CAM13712.1.
BC027102 mRNA. Translation: AAH27102.1.
CCDSiCCDS41098.1. [Q99JA4-1]
RefSeqiNP_001268788.1. NM_001281859.1. [Q99JA4-1]
NP_001268791.1. NM_001281862.1. [Q99JA4-2]
NP_067451.2. NM_021476.5. [Q99JA4-1]
XP_006528275.1. XM_006528212.1. [Q99JA4-2]
UniGeneiMm.287166.

Genome annotation databases

EnsembliENSMUST00000064892; ENSMUSP00000063520; ENSMUSG00000052821. [Q99JA4-1]
ENSMUST00000113480; ENSMUSP00000109108; ENSMUSG00000052821. [Q99JA4-1]
GeneIDi58861.
KEGGimmu:58861.
UCSCiuc009ubt.1. mouse. [Q99JA4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF329272 Genomic DNA. Translation: AAK16715.1.
AF329272 Genomic DNA. Translation: AAK16716.1.
AF205830 mRNA. Translation: AAK15433.1.
AF263370 mRNA. Translation: AAF73047.1.
AB044087 mRNA. Translation: BAA96809.1.
AK033476 mRNA. Translation: BAC28308.1.
AL732460 Genomic DNA. Translation: CAM13712.1.
BC027102 mRNA. Translation: AAH27102.1.
CCDSiCCDS41098.1. [Q99JA4-1]
RefSeqiNP_001268788.1. NM_001281859.1. [Q99JA4-1]
NP_001268791.1. NM_001281862.1. [Q99JA4-2]
NP_067451.2. NM_021476.5. [Q99JA4-1]
XP_006528275.1. XM_006528212.1. [Q99JA4-2]
UniGeneiMm.287166.

3D structure databases

ProteinModelPortaliQ99JA4.
SMRiQ99JA4. Positions 40-331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48916N.

Chemistry

GuidetoPHARMACOLOGYi269.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ99JA4.

Proteomic databases

PaxDbiQ99JA4.
PRIDEiQ99JA4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064892; ENSMUSP00000063520; ENSMUSG00000052821. [Q99JA4-1]
ENSMUST00000113480; ENSMUSP00000109108; ENSMUSG00000052821. [Q99JA4-1]
GeneIDi58861.
KEGGimmu:58861.
UCSCiuc009ubt.1. mouse. [Q99JA4-1]

Organism-specific databases

CTDi10800.
MGIiMGI:1926218. Cysltr1.

Phylogenomic databases

eggNOGiNOG119185.
GeneTreeiENSGT00760000118784.
HOGENOMiHOG000236328.
HOVERGENiHBG099611.
InParanoidiQ99JA4.
KOiK04322.
OMAiPQDNQAK.
OrthoDBiEOG7992R9.
PhylomeDBiQ99JA4.
TreeFamiTF350009.

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_230951. Leukotriene receptors.

Miscellaneous databases

NextBioi314416.
PROiQ99JA4.
SOURCEiSearch...

Gene expression databases

BgeeiQ99JA4.
CleanExiMM_CYSLTR1.
GenevestigatoriQ99JA4.

Family and domain databases

InterProiIPR013310. CLT1_recept.
IPR004071. Cyst_leuk_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01902. CYSLT1RECPTR.
PR01533. CYSLTRECPTR.
PR00237. GPCRRHODOPSN.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification in mice of two isoforms of the cysteinyl leukotriene 1 receptor that result from alternative splicing."
    Maekawa A., Kanaoka Y., Lam B.K., Austen K.F.
    Proc. Natl. Acad. Sci. U.S.A. 98:2256-2261(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
    Strain: 129/Sv.
  2. "Molecular cloning and functional characterization of murine cysteinyl-leukotriene 1 (CysLT1) receptors."
    Martin V., Sawyer N., Stocco R., Unett D., Lerner M.R., Abramovitz M., Funk C.D.
    Biochem. Pharmacol. 62:1193-1200(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Trachea.
  3. "Identification of a murine cysteinyl leukotriene receptor by expression in Xenopus laevis oocytes."
    Mollerup J., Jorgensen S.T., Hougaard C., Hoffmann E.K.
    Biochim. Biophys. Acta 1517:455-459(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Blood.
  4. Ogasawara H., Izumi T., Shimizu T.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: T-cell.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Colon.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II.
    Tissue: Mammary gland.

Entry informationi

Entry nameiCLTR1_MOUSE
AccessioniPrimary (citable) accession number: Q99JA4
Secondary accession number(s): Q544P1, Q9JJ71, Q9JK47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: June 1, 2001
Last modified: January 7, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

MK-571, a selective antagonist, was shown to inhibit eosinophilia, bronchial hyperreactivity and microvascular leakage. Zafirlukast (Accolate) and pranlukast (Onon) were also shown to be selective antagonists.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.