Q99JA4 (CLTR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteinyl leukotriene receptor 1
Short name=CysLTR1

Alternative name(s):
Cysteinyl leukotriene D4 receptor
Short name=LTD4 receptor

Gene names

Name:	Cysltr1
Synonyms:	Cyslt1, Cyslt1r

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for cysteinyl leukotrienes mediating constriction of the microvascular smooth muscle during an inflammatory response. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Widely expressed, with higher expression in the lung and skin, intermediate levels in the heart, kidney and stomach and lower levels in several other tissues. Isoform 1 is the most abundant form in all tested tissues.
Miscellaneous	MK-571, a selective antagonist, was shown to inhibit eosinophilia, bronchial hyperreactivity and microvascular leakage. Zafirlukast (Accolate) and pranlukast (Onon) were also shown to be selective antagonists.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	calcium ion transport Inferred from direct assay PubMed 16002666. Source: MGI chemotaxis Inferred from direct assay PubMed 16002666. Source: MGI inflammatory response Inferred from electronic annotation. Source: Compara positive regulation of angiogenesis Inferred from electronic annotation. Source: Compara positive regulation of vasoconstriction Inferred from electronic annotation. Source: Compara
Cellular_component	integral to plasma membrane Inferred from direct assay Ref.1. Source: MGI
Molecular_function	leukotriene receptor activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99JA4-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99JA4-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Cysteinyl leukotriene receptor 1		PRO_0000069300
Regions
	Topological domain	1 – 43	43	Extracellular Potential
	Transmembrane	44 – 64	21	Helical; Name=1; Potential
	Topological domain	65 – 72	8	Cytoplasmic Potential
	Transmembrane	73 – 93	21	Helical; Name=2; Potential
	Topological domain	94 – 121	28	Extracellular Potential
	Transmembrane	122 – 142	21	Helical; Name=3; Potential
	Topological domain	143 – 156	14	Cytoplasmic Potential
	Transmembrane	157 – 177	21	Helical; Name=4; Potential
	Topological domain	178 – 208	31	Extracellular Potential
	Transmembrane	209 – 229	21	Helical; Name=5; Potential
	Topological domain	230 – 245	16	Cytoplasmic Potential
	Transmembrane	246 – 266	21	Helical; Name=6; Potential
	Topological domain	267 – 291	25	Extracellular Potential
	Transmembrane	292 – 312	21	Helical; Name=7; Potential
	Topological domain	313 – 352	40	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	15	1	N-linked (GlcNAc...) Potential
	Glycosylation	19	1	N-linked (GlcNAc...) Potential
	Glycosylation	26	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Disulfide bond	111 ↔ 188		By similarity
Natural variations
	Alternative sequence	1 – 13	13	Missing in isoform 2.		VSP_001921
Experimental info
	Sequence conflict	176	1	Y → D in AAF73047. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 5BDC94B3F1CD0CAB	FASTA	352	40,715
	10 20 30 40 50 60 MYLQGTKQTF LENMNGTENL TTSLINNTCH DTIDEFRNQV YSTMYSVISV VGFFGNSFVL 70 80 90 100 110 120 YVLIKTYHEK SAFQVYMINL AIADLLCVCT LPLRVVYYVH KGKWLFGDFL CRLTTYALYV 130 140 150 160 170 180 NLYCSIFFMT AMSFFRCVAI VFPVQNINLV TQKKARFVCI GIWIFVILTS SPFLMYKSYQ 190 200 210 220 230 240 DEKNNTKCFE PPQNNQAKKY VLILHYVSLF FGFIIPFVTI IVCYTMIILT LLKNTMKKNM 250 260 270 280 290 300 PSRRKAIGMI IVVTAAFLVS FMPYHIQRTI HLHLLHSETR PCDSVLRMQK SVVITLSLAA 310 320 330 340 350 SNCCFDPLLY FFSGGNFRRR LSTFRKHSLS SMTYVPKKKA SLPEKGEEIC NE
	Isoform 2 (Short) [UniParc]. Checksum: 27BF72C0F3F0F00F	FASTA	339	39,160
	10 20 30 40 50 60 MNGTENLTTS LINNTCHDTI DEFRNQVYST MYSVISVVGF FGNSFVLYVL IKTYHEKSAF 70 80 90 100 110 120 QVYMINLAIA DLLCVCTLPL RVVYYVHKGK WLFGDFLCRL TTYALYVNLY CSIFFMTAMS 130 140 150 160 170 180 FFRCVAIVFP VQNINLVTQK KARFVCIGIW IFVILTSSPF LMYKSYQDEK NNTKCFEPPQ 190 200 210 220 230 240 NNQAKKYVLI LHYVSLFFGF IIPFVTIIVC YTMIILTLLK NTMKKNMPSR RKAIGMIIVV 250 260 270 280 290 300 TAAFLVSFMP YHIQRTIHLH LLHSETRPCD SVLRMQKSVV ITLSLAASNC CFDPLLYFFS 310 320 330 GGNFRRRLST FRKHSLSSMT YVPKKKASLP EKGEEICNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification in mice of two isoforms of the cysteinyl leukotriene 1 receptor that result from alternative splicing." Maekawa A., Kanaoka Y., Lam B.K., Austen K.F. Proc. Natl. Acad. Sci. U.S.A. 98:2256-2261(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2). Strain: 129/Sv.
[2]	"Molecular cloning and functional characterization of murine cysteinyl-leukotriene 1 (CysLT1) receptors." Martin V., Sawyer N., Stocco R., Unett D., Lerner M.R., Abramovitz M., Funk C.D. Biochem. Pharmacol. 62:1193-1200(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Trachea.
[3]	"Identification of a murine cysteinyl leukotriene receptor by expression in Xenopus laevis oocytes." Mollerup J., Jorgensen S.T., Hougaard C., Hoffmann E.K. Biochim. Biophys. Acta 1517:455-459(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Blood.
[4]	Ogasawara H., Izumi T., Shimizu T. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: T-cell.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Colon.
[6]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Czech II. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF329272 Genomic DNA. Translation: AAK16715.1. AF329272 Genomic DNA. Translation: AAK16716.1. AF205830 mRNA. Translation: AAK15433.1. AF263370 mRNA. Translation: AAF73047.1. AB044087 mRNA. Translation: BAA96809.1. AK033476 mRNA. Translation: BAC28308.1. AL732460 Genomic DNA. Translation: CAM13712.1. BC027102 mRNA. Translation: AAH27102.1.
IPI	IPI00229594. IPI00319598.
RefSeq	NP_067451.2. NM_021476.4.
UniGene	Mm.287166.
3D structure databases
ProteinModelPortal	Q99JA4.
SMR	Q99JA4. Positions 40-341.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48916N.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q99JA4.
Proteomic databases
PaxDb	Q99JA4.
PRIDE	Q99JA4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000064892; ENSMUSP00000063520; ENSMUSG00000052821. ENSMUST00000113480; ENSMUSP00000109108; ENSMUSG00000052821.
GeneID	58861.
KEGG	mmu:58861.
UCSC	uc009ubt.1. mouse.
Organism-specific databases
CTD	10800.
MGI	MGI:1926218. Cysltr1.
Phylogenomic databases
eggNOG	NOG119185.
GeneTree	ENSGT00680000099512.
HOGENOM	HOG000236328.
HOVERGEN	HBG099611.
InParanoid	Q99JA4.
KO	K04322.
OrthoDB	EOG46T31Z.
Gene expression databases
Bgee	Q99JA4.
CleanEx	MM_CYSLTR1.
Genevestigator	Q99JA4.
GermOnline	ENSMUSG00000052821. Mus musculus.
Family and domain databases
InterPro	IPR013310. CLT1_recept. IPR004071. Cyst_leuk_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01902. CYSLT1RECPTR. PR01533. CYSLTRECPTR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	314416.
SOURCE	Search...

Entry information

Entry name

CLTR1_MOUSE

Accession

Primary (citable) accession number: Q99JA4
Secondary accession number(s): Q544P1, Q9JJ71, Q9JK47

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program