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Protein

Agouti-signaling protein

Gene

Asip

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment).

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Agouti-signaling protein
Short name:
ASP
Alternative name(s):
Agouti switch protein
Gene namesi
Name:AsipBy similarity
Synonyms:AImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2003. Asip.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000000103123 – 131Agouti-signaling proteinAdd BLAST109

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi39N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi92 ↔ 107PROSITE-ProRule annotation
Disulfide bondi99 ↔ 113PROSITE-ProRule annotation
Disulfide bondi106 ↔ 124PROSITE-ProRule annotation
Disulfide bondi110 ↔ 131PROSITE-ProRule annotation
Disulfide bondi115 ↔ 122PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ99JA2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 131AgoutiPROSITE-ProRule annotationAdd BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi57 – 85Arg/Lys-rich (basic)Add BLAST29

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity

Sequence similaritiesi

Contains 1 agouti domain.PROSITE-ProRule annotation

Keywords - Domaini

Knottin, Signal

Phylogenomic databases

HOGENOMiHOG000111779.
HOVERGENiHBG050593.
InParanoidiQ99JA2.
KOiK08725.
PhylomeDBiQ99JA2.

Family and domain databases

Gene3Di4.10.760.10. 1 hit.
InterProiIPR007733. Agouti.
IPR027300. Agouti_dom.
[Graphical view]
PANTHERiPTHR16551. PTHR16551. 1 hit.
PfamiPF05039. Agouti. 1 hit.
[Graphical view]
SMARTiSM00792. Agouti. 1 hit.
[Graphical view]
SUPFAMiSSF57055. SSF57055. 1 hit.
PROSITEiPS60024. AGOUTI_1. 1 hit.
PS51150. AGOUTI_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99JA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVTRLLLAT LVGFLCFLTV HSHLVFEETL GDDRSLKSNS SINSLDFSSV
60 70 80 90 100
SIVALNKKSK KISRKEAEKR KRSSKKKASI KKVARPPPPS PCVATRDSCK
110 120 130
PPAPACCNPC ASCQCRFFGS ACTCRVLNPN C
Length:131
Mass (Da):14,276
Last modified:June 1, 2001 - v1
Checksum:i6E45BB22F2075AEB
GO

Polymorphismi

A polymorphism exists that is responsible for the nonagouti phenotype, characterized by a plain black coat on the back and belly. This is due to a 19-bp deletion resulting in a frameshift at position 36 and a premature stop codon at position 48.
Both strain WKAH (agouti) and strain BN (nonagouti) contain a substitution at the splice donor site of intron 3, resulting in a shorter isoform lacking exon 3 which causes reduced expression levels in strain WKAH and almost undetectable levels in strain BN.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045587 mRNA. Translation: BAB21564.1.
AB045590 Genomic DNA. Translation: BAB21579.1.
RefSeqiNP_443211.1. NM_052979.2.
UniGeneiRn.205372.

Genome annotation databases

GeneIDi24152.
KEGGirno:24152.
UCSCiRGD:2003. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045587 mRNA. Translation: BAB21564.1.
AB045590 Genomic DNA. Translation: BAB21579.1.
RefSeqiNP_443211.1. NM_052979.2.
UniGeneiRn.205372.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ99JA2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24152.
KEGGirno:24152.
UCSCiRGD:2003. rat.

Organism-specific databases

CTDi434.
RGDi2003. Asip.

Phylogenomic databases

HOGENOMiHOG000111779.
HOVERGENiHBG050593.
InParanoidiQ99JA2.
KOiK08725.
PhylomeDBiQ99JA2.

Miscellaneous databases

PROiQ99JA2.

Family and domain databases

Gene3Di4.10.760.10. 1 hit.
InterProiIPR007733. Agouti.
IPR027300. Agouti_dom.
[Graphical view]
PANTHERiPTHR16551. PTHR16551. 1 hit.
PfamiPF05039. Agouti. 1 hit.
[Graphical view]
SMARTiSM00792. Agouti. 1 hit.
[Graphical view]
SUPFAMiSSF57055. SSF57055. 1 hit.
PROSITEiPS60024. AGOUTI_1. 1 hit.
PS51150. AGOUTI_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASIP_RAT
AccessioniPrimary (citable) accession number: Q99JA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.