Q99JA0 (CALCA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcitonin gene-related peptide 1

Alternative name(s):
Alpha-type CGRP
Calcitonin gene-related peptide I
Short name=CGRP-I

Gene names

Name:	Calca
Synonyms:	Calc

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	128 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP By similarity.
Subcellular location	Secreted.
Tissue specificity	Detected in nerve cells of cerebrum, hippocampus and pons/midbrain in newborns, and only in nerve cells of pons/midbrain in adult. Ref.4
Sequence similarities	Belongs to the calcitonin family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of adenylate cyclase activity Inferred from direct assay PubMed 8582325. Source: MGI adenylate cyclase-activating G-protein coupled receptor signaling pathway Inferred from genetic interaction PubMed 8582325. Source: MGI cellular calcium ion homeostasis Inferred from genetic interaction PubMed 12488435. Source: MGI detection of temperature stimulus involved in sensory perception of pain Inferred from mutant phenotype PubMed 16118273. Source: MGI feeding behavior Inferred from direct assay PubMed 8735980. Source: MGI inflammatory response Inferred from direct assay PubMed 7698182. Source: MGI negative regulation of blood pressure Inferred from mutant phenotype PubMed 10642343PubMed 11717154PubMed 15583078. Source: MGI negative regulation of neurological system process Inferred from mutant phenotype PubMed 11717154. Source: MGI negative regulation of ossification Inferred from mutant phenotype PubMed 12488435PubMed 15537449. Source: MGI negative regulation of smooth muscle contraction Inferred from direct assay PubMed 9322931. Source: MGI neuropeptide signaling pathway Inferred from direct assay PubMed 7698182. Source: MGI positive regulation of ossification Inferred from mutant phenotype PubMed 15537449. Source: MGI regulation of heart rate Inferred from mutant phenotype PubMed 11717154. Source: MGI response to heat Inferred from mutant phenotype PubMed 11166483PubMed 16118273. Source: MGI response to pain Inferred from mutant phenotype PubMed 10208559PubMed 11276224. Source: MGI sperm capacitation Inferred from sequence alignment PubMed 12704735. Source: MGI vasodilation of artery involved in baroreceptor response to increased systemic arterial blood pressure Inferred from mutant phenotype PubMed 11717154. Source: MGI
Cellular_component	cytoplasm Inferred from direct assay PubMed 11551509PubMed 14765981PubMed 9271704. Source: MGI extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from direct assay PubMed 14765981. Source: MGI neuronal cell body Inferred from direct assay PubMed 9838101. Source: MGI terminal bouton Inferred from direct assay PubMed 10208559PubMed 10532808PubMed 8453761PubMed 9853118. Source: MGI
Molecular_function	calcitonin receptor binding Inferred from direct assay PubMed 9322931. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Calcitonin-gene related peptide I (identifier: Q99JA0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Calcitonin (identifier: P70160-1)

The sequence of this isoform can be found in the external entry P70160.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 80	55	By similarity		PRO_0000004059
	Peptide	83 – 119	37	Calcitonin gene-related peptide 1		PRO_0000004060
	Propeptide	125 – 128	4	By similarity		PRO_0000004061
Amino acid modifications
	Modified residue	119	1	Phenylalanine amide By similarity
	Disulfide bond	84 ↔ 89		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform Calcitonin-gene related peptide I [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 83BB0E36C8B4239E	FASTA	128	14,065
	10 20 30 40 50 60 MGFLKFSPFL VVSILLLYQA CSLQAVPLRS ILESSPGMAT LSEEEVRLLA ALVQDYMQMK 70 80 90 100 110 120 ARELEQEEEQ EAEGSSVTAQ KRSCNTATCV THRLAGLLSR SGGVVKDNFV PTNVGSEAFG RRRRDLQA
	Isoform Calcitonin [UniParc]. See P70160.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse CGRP precursor is highly homologous to that of the rat." Sarasa M., Catalan J., Aramayona J., Sorribas V. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure of the mouse calcitonin/calcitonin gene-related peptide alpha and beta genes." Thomas P.M., Nasonkin I., Zhang H., Gagel R.F., Cote G.J. DNA Seq. 12:131-135(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
[4]	"Genomic organization, expression and evolution of porcine CRSP1, 2, and 3." Rezaeian A.H., Katafuchi T., Yoshizawa M., Hiraiwa N., Saito T., Nishibori M., Hamano K., Minamino N., Yasue H. Cytogenet. Genome Res. 121:41-49(2008) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF330212 mRNA. Translation: AAK06841.1. AF325522, AF325521 Genomic DNA. Translation: AAK18181.1. BC028771 mRNA. Translation: AAH28771.1.
IPI	IPI00115080.
RefSeq	NP_001029126.1. NM_001033954.3.
UniGene	Mm.4361.
3D structure databases
ProteinModelPortal	Q99JA0.
SMR	Q99JA0. Positions 83-119.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000032907.
Proteomic databases
PRIDE	Q99JA0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032906; ENSMUSP00000032906; ENSMUSG00000030669.
GeneID	12310.
KEGG	mmu:12310.
UCSC	uc009jif.2. mouse.
Organism-specific databases
CTD	796.
MGI	MGI:2151253. Calca.
Phylogenomic databases
eggNOG	NOG40787.
GeneTree	ENSGT00390000010511.
HOGENOM	HOG000038203.
HOVERGEN	HBG031362.
OMA	EQETQGS.
OrthoDB	EOG43N7F5.
Gene expression databases
ArrayExpress	Q99JA0.
Bgee	Q99JA0.
CleanEx	MM_CALCA.
Genevestigator	Q99JA0.
GermOnline	ENSMUSG00000030669. Mus musculus.
Family and domain databases
InterPro	IPR018360. Calcitonin_CS. IPR001693. Calcitonin_peptide-like. IPR021117. Procalcitonin-like. IPR021116. Procalcitonin/adrenomedullin. IPR015476. Procalcitonin_gene-rel_peptide. [Graphical view]
PANTHER	PTHR10505. PTHR10505. 1 hit. PTHR10505:SF2. PTHR10505:SF2. 1 hit.
Pfam	PF00214. Calc_CGRP_IAPP. 1 hit. [Graphical view]
PRINTS	PR00817. CALCITONINB.
SMART	SM00113. CALCITONIN. 1 hit. [Graphical view]
PROSITE	PS00258. CALCITONIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CALCA. mouse.
NextBio	280856.
SOURCE	Search...

Entry information

Entry name

CALCA_MOUSE

Accession

Primary (citable) accession number: Q99JA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program