Reviewed, UniProtKB/Swiss-Prot Q99J95 (CDK9_MOUSE)

Names and origin

Protein names

Recommended name:
    Cell division protein kinase 9
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    Cyclin-dependent kinase 9

Gene names

Name:

Cdk9

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subunit structure	Associates with CCNT1/cyclin-T1 to form P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Also associates with CCNK/cyclin-K. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed at high levels in brain and kidney. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence BAE25966.1 differs from that shown. Reason: Frameshift at position 46.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC snRNA binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q99J95-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q99J95-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q99J95-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-129: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Cell division protein kinase 9		PRO_0000085801
Regions
	Domain	19 – 315	297	Protein kinase
	Nucleotide binding	25 – 33	9	ATP By similarity
Sites
	Active site	149	1	Proton acceptor By similarity
	Binding site	48	1	ATP By similarity
Amino acid modifications
	Modified residue	186	1	Phosphothreonine By similarity
	Modified residue	347	1	Phosphoserine By similarity
	Modified residue	353	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 129	129	Missing in isoform 3.		VSP_016290
	Alternative sequence	1 – 51	51	Missing in isoform 2.		VSP_016289
Experimental info
	Sequence conflict	122	1	T → R in BAE34054. Ref.3
	Sequence conflict	154	1	N → S in BAE25055. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 973B18869F9963E3	FASTA	372	42,762
	10 20 30 40 50 60 MAKQYDSVEC PFCDEVTKYE KLAKIGQGTF GEVFKAKHRQ TGQKVALKKV LMENEKEGFP 70 80 90 100 110 120 ITALREIKIL QLLKHENVVN LIEICRTKAS PYNRCKGSIY LVFDFCEHDL AGLLSNVLVK 130 140 150 160 170 180 FTLSEIKRVM QMLLNGLYYI HRNKILHRDM KAANVLITRD GVLKLADFGL ARAFSLAKNS 190 200 210 220 230 240 QPNRYTNRVV TLWYRPPELL LGERDYGPPI DLWGAGCIMA EMWTRSPIMQ GNTEQHQLAL 250 260 270 280 290 300 ISQLCGSITP EVWPNVDKYE LFEKLELVKG QKRKVKDRLK AYVRDPYALD LIDKLLVLDP 310 320 330 340 350 360 AQRIDSDDAL NHDFFWSDPM PSDLKGMLST HLTSMFEYLA PPRRKGSQIT QQSTNQSRNP 370 ATTNQTEFER VF
	Isoform 2. Checksum: 4E7C2E2B4DB2042A	FASTA	321	37,002
	10 20 30 40 50 60 MENEKEGFPI TALREIKILQ LLKHENVVNL IEICRTKASP YNRCKGSIYL VFDFCEHDLA 70 80 90 100 110 120 GLLSNVLVKF TLSEIKRVMQ MLLNGLYYIH RNKILHRDMK AANVLITRDG VLKLADFGLA 130 140 150 160 170 180 RAFSLAKNSQ PNRYTNRVVT LWYRPPELLL GERDYGPPID LWGAGCIMAE MWTRSPIMQG 190 200 210 220 230 240 NTEQHQLALI SQLCGSITPE VWPNVDKYEL FEKLELVKGQ KRKVKDRLKA YVRDPYALDL 250 260 270 280 290 300 IDKLLVLDPA QRIDSDDALN HDFFWSDPMP SDLKGMLSTH LTSMFEYLAP PRRKGSQITQ 310 320 QSTNQSRNPA TTNQTEFERV F
	Isoform 3. Checksum: 99DBB3953EB6971A	FASTA	243	28,026
	10 20 30 40 50 60 MQMLLNGLYY IHRNKILHRD MKAANVLITR DGVLKLADFG LARAFSLAKN SQPNRYTNRV 70 80 90 100 110 120 VTLWYRPPEL LLGERDYGPP IDLWGAGCIM AEMWTRSPIM QGNTEQHQLA LISQLCGSIT 130 140 150 160 170 180 PEVWPNVDKY ELFEKLELVK GQKRKVKDRL KAYVRDPYAL DLIDKLLVLD PAQRIDSDDA 190 200 210 220 230 240 LNHDFFWSDP MPSDLKGMLS THLTSMFEYL APPRRKGSQI TQQSTNQSRN PATTNQTEFE RVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of murine CDK9/PITALRE and its tissue-specific expression in development." Bagella L., MacLachlan T.K., Buono R.J., Pisano M.M., Giordano A., De Luca A. J. Cell. Physiol. 177:206-213(1998) [PubMed: 9766517] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Genomic organization, promoter analysis, and chromosomal mapping of the mouse gene encoding Cdk9." Bagella L., Stiegler P., De Luca A., Siracusa L.D., Giordano A. J. Cell. Biochem. 78:170-178(2000) [PubMed: 10797576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6J and NOD. Tissue: Eye, Lung and Spleen.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF327431 mRNA. Translation: AAK15699.1. AF327569 Genomic DNA. Translation: AAK15706.1. AK089276 mRNA. Translation: BAC40824.1. AK142397 mRNA. Translation: BAE25055.1. AK143217 mRNA. Translation: BAE25312.1. AK144607 mRNA. Translation: BAE25966.1. Frameshift. AK157340 mRNA. Translation: BAE34054.1. AL772271 Genomic DNA. Translation: CAQ13017.1. BC003901 mRNA. Translation: AAH03901.1.
IPI	IPI00114953. IPI00656169. IPI00656171.
RefSeq	NP_570930.1.
UniGene	Mm.27557
3D structure databases
HSSP	HSSP built from PDB template 1H4L based on UniProtKB Q00535.
ModBase	Search...
Protein-protein interaction databases
STRING	Q99J95.
PTM databases
PhosphoSite	Q99J95.
Proteomic databases
PRIDE	Q99J95.
Genome annotation databases
Ensembl	ENSMUST00000009699; ENSMUSP00000009699; ENSMUSG00000009555; Mus musculus. [Genome view]
GeneID	107951.
KEGG	mmu:107951.
UCSC	uc008jgn.1. mouse. uc008jgo.1. mouse.
Organism-specific databases
CTD	107951.
MGI	MGI:1328368. Cdk9.
Phylogenomic databases
HOGENOM	Q99J95.
HOVERGEN	Q99J95.
OMA	TEQHQLT.
Enzyme and pathway databases
BRENDA	2.7.11.22. 244. 2.7.11.23. 244.
Gene expression databases
ArrayExpress	Q99J95.
Bgee	Q99J95.
CleanEx	MM_CDK9.
Genevestigator	Q99J95.
GermOnline	ENSMUSG00000009555. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	359767.
SOURCE	Search...

Entry information

Entry name

CDK9_MOUSE

Accession

Primary (citable) accession number: Q99J95
Secondary accession number(s): B0R020 , Q3U002, Q3UMY2, Q3UPT3, Q3UQI6, Q8BTN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)