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Protein

Attractin

Gene

Atrn

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the initial immune cell clustering during inflammatory response and may regulate chemotactic activity of chemokines (By similarity). May play a role in melanocortin signaling pathways that regulate energy homeostasis and hair color. Low-affinity receptor for agouti (By similarity). Has a critical role in normal myelination in the central nervous system.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • cerebellum development Source: Ensembl
  • inflammatory response Source: UniProtKB-KW
  • myelination Source: Ensembl
  • pigmentation Source: CACAO
  • regulation of multicellular organism growth Source: Ensembl
  • response to oxidative stress Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Inflammatory response

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Attractin
Alternative name(s):
Protein zitter
Gene namesi
Name:AtrnImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi69063. Atrn.

Subcellular locationi

Isoform 1 :
  • Cell membrane By similarity; Single-pass type I membrane protein By similarity
Isoform 2 :
  • Secreted By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini87 – 12821196ExtracellularSequence analysisAdd
BLAST
Transmembranei1283 – 130321HelicalSequence analysisAdd
BLAST
Topological domaini1304 – 1432129CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: RGD
  • extracellular exosome Source: Ensembl
  • extracellular space Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Involvement in diseasei

Defects in Atrn (isoform 1) are the cause of the autosomal recessive phenotype zitter (zi), which is characterized by progressive hypomyelination and vacuolation in the central nervous system resulting in early-onset tremor and progressive flaccid paresis of the hind limb. This is due to an 8-bp deletion at the splice donor site of intron 12, which results in aberrant and unstable transcripts.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Propeptidei24 – 8663By similarityPRO_0000394773Add
BLAST
Chaini87 – 14321346AttractinPRO_0000007485Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi104 ↔ 114By similarity
Disulfide bondi108 ↔ 121By similarity
Disulfide bondi123 ↔ 132By similarity
Disulfide bondi135 ↔ 161By similarity
Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence analysis
Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence analysis
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence analysis
Disulfide bondi253 ↔ 263By similarity
Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence analysis
Disulfide bondi257 ↔ 274By similarity
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence analysis
Disulfide bondi276 ↔ 285By similarity
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence analysis
Glycosylationi328 – 3281N-linked (GlcNAc...)Sequence analysis
Glycosylationi365 – 3651N-linked (GlcNAc...)Sequence analysis
Glycosylationi386 – 3861N-linked (GlcNAc...)Sequence analysis
Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence analysis
Glycosylationi431 – 4311N-linked (GlcNAc...)Sequence analysis
Glycosylationi578 – 5781N-linked (GlcNAc...)Sequence analysis
Glycosylationi626 – 6261N-linked (GlcNAc...)Sequence analysis
Glycosylationi734 – 7341N-linked (GlcNAc...)Sequence analysis
Disulfide bondi819 ↔ 921By similarity
Glycosylationi866 – 8661N-linked (GlcNAc...)Sequence analysis
Glycosylationi917 – 9171N-linked (GlcNAc...)Sequence analysis
Glycosylationi926 – 9261N-linked (GlcNAc...)Sequence analysis
Glycosylationi989 – 9891N-linked (GlcNAc...)Sequence analysis
Glycosylationi1046 – 10461N-linked (GlcNAc...)Sequence analysis
Glycosylationi1057 – 10571N-linked (GlcNAc...)Sequence analysis
Disulfide bondi1066 ↔ 1074By similarity
Disulfide bondi1068 ↔ 1080By similarity
Glycosylationi1076 – 10761N-linked (GlcNAc...)Sequence analysis
Disulfide bondi1083 ↔ 1092By similarity
Disulfide bondi1095 ↔ 1109By similarity
Disulfide bondi1112 ↔ 1121By similarity
Disulfide bondi1114 ↔ 1128By similarity
Disulfide bondi1130 ↔ 1140By similarity
Disulfide bondi1143 ↔ 1158By similarity
Glycosylationi1201 – 12011N-linked (GlcNAc...)Sequence analysis
Glycosylationi1209 – 12091N-linked (GlcNAc...)Sequence analysis
Glycosylationi1253 – 12531N-linked (GlcNAc...)Sequence analysis
Glycosylationi1262 – 12621N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Heavily glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ99J86.
PRIDEiQ99J86.

PTM databases

PhosphoSiteiQ99J86.
SwissPalmiQ99J86.
UniCarbKBiQ99J86.

Expressioni

Gene expression databases

GenevisibleiQ99J86. RN.

Interactioni

Subunit structurei

Monomer and homotrimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028847.

Structurei

3D structure databases

ProteinModelPortaliQ99J86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 13334EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini135 – 251117CUBPROSITE-ProRule annotationAdd
BLAST
Domaini249 – 28638EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Repeati355 – 40551Kelch 1Sequence analysisAdd
BLAST
Repeati406 – 45449Kelch 2Sequence analysisAdd
BLAST
Repeati464 – 51148Kelch 3Sequence analysisAdd
BLAST
Repeati516 – 56752Kelch 4Sequence analysisAdd
BLAST
Repeati569 – 62759Kelch 5Sequence analysisAdd
BLAST
Repeati628 – 67447Kelch 6Sequence analysisAdd
BLAST
Domaini706 – 75146PSI 1Add
BLAST
Domaini758 – 79740PSI 2Add
BLAST
Domaini798 – 922125C-type lectinPROSITE-ProRule annotationAdd
BLAST
Domaini935 – 98652PSI 3Add
BLAST
Domaini989 – 106476PSI 4Add
BLAST
Domaini1066 – 111146Laminin EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini1112 – 116049Laminin EGF-like 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi932 – 1143212Cys-richAdd
BLAST

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 CUB domain.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 6 Kelch repeats.Sequence analysis
Contains 2 laminin EGF-like domains.PROSITE-ProRule annotation
Contains 4 PSI domains.Curated

Keywords - Domaini

EGF-like domain, Kelch repeat, Laminin EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1388. Eukaryota.
ENOG410XRW4. LUCA.
GeneTreeiENSGT00390000001118.
HOGENOMiHOG000231727.
HOVERGENiHBG004312.
InParanoidiQ99J86.
OMAiSCPPENC.
OrthoDBiEOG7D59MM.
PhylomeDBiQ99J86.
TreeFamiTF321873.

Family and domain databases

Gene3Di2.120.10.80. 2 hits.
2.60.120.290. 1 hit.
3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR000859. CUB_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR002049. Laminin_EGF.
IPR016201. Plexin-like_fold.
IPR002165. Plexin_repeat.
[Graphical view]
PfamiPF00431. CUB. 1 hit.
PF01344. Kelch_1. 2 hits.
PF00059. Lectin_C. 1 hit.
PF01437. PSI. 2 hits.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
SM00042. CUB. 1 hit.
SM00181. EGF. 3 hits.
SM00180. EGF_Lam. 2 hits.
SM00423. PSI. 5 hits.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
PS01180. CUB. 1 hit.
PS00022. EGF_1. 3 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS01248. EGF_LAM_1. 1 hit.
PS50027. EGF_LAM_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q99J86-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVAAAAAAEA TEARLRGYTT ATAAPAGWKE RQHRPCAATG AWRPWPRAGL
60 70 80 90 100
CLPRVLSRAL SPPPLLPLLP LLFSLLLLPL PREAEAAAVA AAVSGSAAAE
110 120 130 140 150
AKECDRPCVN GGRCNPGTGQ CVCPTGWVGE QCQHCGGRFR LTGSSGFVTD
160 170 180 190 200
GPGNYKYKTK CTWLIEGQPN KIMRLRFNHF ATECSWDHLY VYDGDSIYAP
210 220 230 240 250
LIAAFSGLIV PERDGNETAP EVTVTSGYAL LHFFSDAAYN LTGFNITYNF
260 270 280 290 300
DMCPNNCSGR GECKSSNSSS TVECECSENW KGESCDIPHC TDNCGFPHRG
310 320 330 340 350
ICNASDTRGC SCFPHWQGPG CSIPVPANQS FWTREEYSDL KLPRASHKAV
360 370 380 390 400
VNGNIMWVVG GYMFNHSDYS MVLAYDLASR EWLSLNHSVN SVVVRYGHSL
410 420 430 440 450
ALHKDKIYMY GGKIDSTGNV TNELRVFHIH NESWVLLTPK AKDQYAVVGH
460 470 480 490 500
SAHIVTLSSG RVVMLVIFGH CPLYGYISVV QEYDLEKNTW SILQTQGALV
510 520 530 540 550
QGGYGHSSVY DHRTKALYVH GGYKAFSANK YRLADDLYRY HVDTQMWTIL
560 570 580 590 600
KDSRFFRYLH TAVIVSGTML VFGGNTHNDT SMSHGAKCFS SDFMAYDIAC
610 620 630 640 650
DRWSVLPRPE LHHDVNRFGH SAVLHNSTMY VFGGFNSLLL SDVLVFTSEQ
660 670 680 690 700
CDAHRSEAAC VAAGPGIRCL WDTQSSRCTS WELATEEQAE KLKSECFSKR
710 720 730 740 750
TLDHDRCDQH TDCYSCTANT NDCHWCNDHC VPVNHSCTEG QISIAKYDNC
760 770 780 790 800
PKDNPMYYCN KKTSCRSCAL DQNCQWEPRN QECIALPENI CGIGWHLVGN
810 820 830 840 850
SCLKITTAKE NYDNAKLSCR NHNAFLASLT SQKKVEFVLK QLRLMQSSQS
860 870 880 890 900
TSKLTLTPWV GLRKINVSYW CWEDMSPFTN SLLQWMPSEP SDAGFCGILS
910 920 930 940 950
EPSTRGLKAA TCINPLNGSV CERPANHSAK QCRTPCALRT ACGECTSSSS
960 970 980 990 1000
ECMWCSNMKQ CVDSNAYVAS FPFGQCMEWY TMSSCPPENC SGYCTCSHCL
1010 1020 1030 1040 1050
EQPGCGWCTD PSNTGKGKCI EGSYKGPVKM PSHASTGNVY PQPLLNSSMC
1060 1070 1080 1090 1100
LEDSRYNWSF IHCPACQCNG HSKCINQSIC EKCEDLTTGK HCETCISGFY
1110 1120 1130 1140 1150
GDPTNGGKCQ PCKCNGHASL CNTNTGKCFC TTKGVKGEEC QLCEVENRYQ
1160 1170 1180 1190 1200
GNPLKGTCYY TLLIDYQFTF SLSQEDDRYY TAINFVATPD EQNRDLDMFI
1210 1220 1230 1240 1250
NASKNFNLNI TWATSFPAGT QTGEEVPVVS KTNIKEYKDS FSNEKFDFRN
1260 1270 1280 1290 1300
HPNITFFVYV SNFTWPIKIQ IAFSQHSNFM DLVQFFVTFF SCFLSLLLVA
1310 1320 1330 1340 1350
AVVWKIKQSC WASRRREQLL REMQQMASRP FASVNVALET DEEPPDLIGG
1360 1370 1380 1390 1400
SIKTVPKPIA LEPCFGNKAA VLSVFVRLPR GLGGIPPPGQ SGLAVASALV
1410 1420 1430
DISQQMPIVY KEKSGAVRNR KQQPPAQPGT CI
Length:1,432
Mass (Da):158,673
Last modified:June 1, 2001 - v1
Checksum:iDE4177EE5D55E866
GO
Isoform 21 Publication (identifier: Q99J86-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1271-1275: IAFSQ → VRVTS
     1276-1432: Missing.

Show »
Length:1,275
Mass (Da):141,522
Checksum:iCED768A4E33B50C5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti350 – 3501V → E in BAB21018 (PubMed:11209055).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1271 – 12755IAFSQ → VRVTS in isoform 2. 1 PublicationVSP_051674
Alternative sequencei1276 – 1432157Missing in isoform 2. 1 PublicationVSP_051675Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038387 mRNA. Translation: BAB21017.1.
AB038388 mRNA. Translation: BAB21018.1.
AB049248 Genomic DNA. Translation: BAB21058.1.
RefSeqiNP_112641.1. NM_031351.1. [Q99J86-1]
UniGeneiRn.53846.

Genome annotation databases

EnsembliENSRNOT00000028847; ENSRNOP00000028847; ENSRNOG00000021240. [Q99J86-1]
GeneIDi83526.
KEGGirno:83526.
UCSCiRGD:69063. rat. [Q99J86-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038387 mRNA. Translation: BAB21017.1.
AB038388 mRNA. Translation: BAB21018.1.
AB049248 Genomic DNA. Translation: BAB21058.1.
RefSeqiNP_112641.1. NM_031351.1. [Q99J86-1]
UniGeneiRn.53846.

3D structure databases

ProteinModelPortaliQ99J86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028847.

PTM databases

PhosphoSiteiQ99J86.
SwissPalmiQ99J86.
UniCarbKBiQ99J86.

Proteomic databases

PaxDbiQ99J86.
PRIDEiQ99J86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028847; ENSRNOP00000028847; ENSRNOG00000021240. [Q99J86-1]
GeneIDi83526.
KEGGirno:83526.
UCSCiRGD:69063. rat. [Q99J86-1]

Organism-specific databases

CTDi8455.
RGDi69063. Atrn.

Phylogenomic databases

eggNOGiKOG1388. Eukaryota.
ENOG410XRW4. LUCA.
GeneTreeiENSGT00390000001118.
HOGENOMiHOG000231727.
HOVERGENiHBG004312.
InParanoidiQ99J86.
OMAiSCPPENC.
OrthoDBiEOG7D59MM.
PhylomeDBiQ99J86.
TreeFamiTF321873.

Miscellaneous databases

NextBioi616013.
PROiQ99J86.

Gene expression databases

GenevisibleiQ99J86. RN.

Family and domain databases

Gene3Di2.120.10.80. 2 hits.
2.60.120.290. 1 hit.
3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR000859. CUB_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR002049. Laminin_EGF.
IPR016201. Plexin-like_fold.
IPR002165. Plexin_repeat.
[Graphical view]
PfamiPF00431. CUB. 1 hit.
PF01344. Kelch_1. 2 hits.
PF00059. Lectin_C. 1 hit.
PF01437. PSI. 2 hits.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
SM00042. CUB. 1 hit.
SM00181. EGF. 3 hits.
SM00180. EGF_Lam. 2 hits.
SM00423. PSI. 5 hits.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
PS01180. CUB. 1 hit.
PS00022. EGF_1. 3 hits.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS01248. EGF_LAM_1. 1 hit.
PS50027. EGF_LAM_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Attractin/mahogany/zitter plays a critical role in myelination of the central nervous system."
    Kuramoto T., Kitada K., Inui T., Sasaki Y., Ito K., Hase T., Kawaguchi S., Ogawa Y., Nakao K., Barsh G.S., Nagao M., Ushijima T., Serikawa T.
    Proc. Natl. Acad. Sci. U.S.A. 98:559-564(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, DISEASE.
    Strain: WTCImported.
    Tissue: Brain1 Publication.

Entry informationi

Entry nameiATRN_RAT
AccessioniPrimary (citable) accession number: Q99J86
Secondary accession number(s): Q99PW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: June 1, 2001
Last modified: May 11, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.