UniProtKB - Q99J83 (ATG5_MOUSE)
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Protein
Autophagy protein 5
Gene
Atg5
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.9 Publications
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD (By similarity).By similarity
(Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection.1 Publication
GO - Biological processi
- aggrephagy Source: ParkinsonsUK-UCL
- antigen processing and presentation of endogenous antigen Source: MGI
- apoptotic process Source: UniProtKB-KW
- autophagosome assembly Source: UniProtKB
- autophagy Source: UniProtKB
- autophagy of host cells involved in interaction with symbiont Source: MGI
- autophagy of mitochondrion Source: ParkinsonsUK-UCL
- autophagy of nucleus Source: GO_Central
- blood vessel remodeling Source: MGI
- cellular homeostasis Source: MGI
- cellular response to nitrogen starvation Source: GO_Central
- cellular response to nitrosative stress Source: ParkinsonsUK-UCL
- cellular response to starvation Source: MGI
- heart contraction Source: MGI
- macroautophagy Source: MGI
- negative regulation of apoptotic process Source: ParkinsonsUK-UCL
- negative regulation of cell death Source: MGI
- negative regulation of histone H4-K16 acetylation Source: MGI
- negative regulation of phagocytosis Source: MGI
- negative regulation of protein ubiquitination Source: MGI
- negative regulation of reactive oxygen species metabolic process Source: ParkinsonsUK-UCL
- negative stranded viral RNA replication Source: MGI
- negative thymic T cell selection Source: MGI
- otolith development Source: MGI
- positive regulation of mucus secretion Source: MGI
- post-translational protein modification Source: UniProtKB
- protein lipidation involved in autophagosome assembly Source: MGI
- regulation of cilium assembly Source: UniProtKB
- regulation of cytokine secretion involved in immune response Source: MGI
- regulation of reactive oxygen species metabolic process Source: MGI
- regulation of release of sequestered calcium ion into cytosol Source: MGI
- response to drug Source: MGI
- response to fungus Source: MGI
- vasodilation Source: MGI
- ventricular cardiac muscle cell development Source: MGI
Keywordsi
Biological process | Apoptosis, Autophagy, Immunity |
Enzyme and pathway databases
Reactomei | R-MMU-1632852. Macroautophagy. R-MMU-5205685. Pink/Parkin Mediated Mitophagy. R-MMU-8934903. Receptor Mediated Mitophagy. |
Names & Taxonomyi
Protein namesi | Recommended name: Autophagy protein 5Alternative name(s): APG5-like |
Gene namesi | Name:Atg5 Synonyms:Apg5l |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1277186. Atg5. |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 130 | K → R: Loss of conjugation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218995 | 1 – 275 | Autophagy protein 5Add BLAST | 275 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Cross-linki | 130 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12)By similarity |
Post-translational modificationi
Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
Acetylated by EP300.By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Ubl conjugationProteomic databases
EPDi | Q99J83. |
MaxQBi | Q99J83. |
PaxDbi | Q99J83. |
PeptideAtlasi | Q99J83. |
PRIDEi | Q99J83. |
PTM databases
iPTMneti | Q99J83. |
PhosphoSitePlusi | Q99J83. |
Expressioni
Tissue specificityi
Ubiquitous.
Gene expression databases
Bgeei | ENSMUSG00000038160. |
CleanExi | MM_ATG5. |
Genevisiblei | Q99J83. MM. |
Interactioni
Subunit structurei
Forms a conjugate with ATG12 (PubMed:11266458, PubMed:12890687, PubMed:12665549, PubMed:18768753, PubMed:19417210). The ATG5-ATG12 conjugate forms a complex with several units of ATG16L (PubMed:12665549). Interacts with TECPR1; the interaction is direct and does not take place when ATG16L is associated with the ATG5-ATG12 conjugate (By similarity). Interacts with DHX58/RIG-1, IFIH1/MDA5 and MAVS/IPS-1 in monomeric form as well as in ATG12-ATG5 conjugate form. The interaction with MAVS is further enhanced upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Interacts with ATG3 (By similarity). Interacts with ATG7 and ATG10 (PubMed:12482611). Interacts with FADD (By similarity).By similarity7 Publications
Binary interactionsi
Protein-protein interaction databases
BioGridi | 198146. 7 interactors. |
CORUMi | Q99J83. |
DIPi | DIP-46420N. |
IntActi | Q99J83. 13 interactors. |
MINTi | Q99J83. |
STRINGi | 10090.ENSMUSP00000044769. |
Structurei
3D structure databases
ProteinModelPortali | Q99J83. |
SMRi | Q99J83. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATG5 family.Curated
Phylogenomic databases
eggNOGi | KOG2976. Eukaryota. ENOG410XQ71. LUCA. |
GeneTreei | ENSGT00390000004766. |
HOGENOMi | HOG000007893. |
HOVERGENi | HBG018731. |
InParanoidi | Q99J83. |
KOi | K08339. |
OMAi | WHYPIGV. |
OrthoDBi | EOG091G0JU7. |
PhylomeDBi | Q99J83. |
TreeFami | TF314415. |
Family and domain databases
InterProi | View protein in InterPro IPR007239. Atg5. |
PANTHERi | PTHR13040. PTHR13040. 1 hit. |
Pfami | View protein in Pfam PF04106. APG5. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q99J83-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD
60 70 80 90 100
KVKKHFQKVM RQEDVSEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI
110 120 130 140 150
TVHFKSFPEK DLLHCPSKDA VEAHFMSCMK EADALKHKSQ VINEMQKKDH
160 170 180 190 200
KQLWMGLQND RFDQFWAINR KLMEYPPEEN GFRYIPFRIY QTTTERPFIQ
210 220 230 240 250
KLFRPVAADG QLHTLGDLLR EVCPSAVAPE DGEKRSQVMI HGIEPMLETP
260 270
LQWLSEHLSY PDNFLHISIV PQPTD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB048349 mRNA. Translation: BAB33383.1. AK028315 mRNA. Translation: BAC25874.1. BC002166 mRNA. Translation: AAH02166.1. |
CCDSi | CCDS23824.1. |
RefSeqi | NP_001300942.1. NM_001314013.1. NP_444299.1. NM_053069.6. XP_011241410.1. XM_011243108.2. |
UniGenei | Mm.22264. |
Genome annotation databases
Ensembli | ENSMUST00000039286; ENSMUSP00000044769; ENSMUSG00000038160. |
GeneIDi | 11793. |
KEGGi | mmu:11793. |
UCSCi | uc007ezt.1. mouse. |
Similar proteinsi
Entry informationi
Entry namei | ATG5_MOUSE | |
Accessioni | Q99J83Primary (citable) accession number: Q99J83 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 16, 2001 |
Last sequence update: | June 1, 2001 | |
Last modified: | March 28, 2018 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |