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Protein

Autophagy protein 5

Gene

Atg5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.9 Publications
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD (By similarity).By similarity

GO - Biological processi

  • aggrephagy Source: ParkinsonsUK-UCL
  • antigen processing and presentation of endogenous antigen Source: MGI
  • apoptotic process Source: UniProtKB-KW
  • autophagosome assembly Source: UniProtKB
  • autophagy Source: UniProtKB
  • blood vessel remodeling Source: MGI
  • cellular homeostasis Source: MGI
  • cellular response to nitrogen starvation Source: GO_Central
  • cellular response to nitrosative stress Source: ParkinsonsUK-UCL
  • cellular response to starvation Source: MGI
  • heart contraction Source: MGI
  • innate immune response Source: UniProtKB-KW
  • macroautophagy Source: MGI
  • mitophagy Source: ParkinsonsUK-UCL
  • negative regulation of apoptotic process Source: ParkinsonsUK-UCL
  • negative regulation of cell death Source: MGI
  • negative regulation of histone H4-K16 acetylation Source: MGI
  • negative regulation of phagocytosis Source: MGI
  • negative regulation of protein ubiquitination Source: MGI
  • negative regulation of reactive oxygen species metabolic process Source: ParkinsonsUK-UCL
  • negative regulation of type I interferon production Source: Reactome
  • negative stranded viral RNA replication Source: MGI
  • negative thymic T cell selection Source: MGI
  • nucleophagy Source: GO_Central
  • otolith development Source: MGI
  • positive regulation of mitophagy Source: Ensembl
  • positive regulation of mucus secretion Source: MGI
  • post-translational protein modification Source: UniProtKB
  • protein lipidation involved in autophagosome assembly Source: MGI
  • regulation of cilium assembly Source: UniProtKB
  • regulation of cytokine secretion involved in immune response Source: MGI
  • regulation of reactive oxygen species metabolic process Source: MGI
  • regulation of release of sequestered calcium ion into cytosol Source: MGI
  • response to drug Source: MGI
  • response to fungus Source: MGI
  • vasodilation Source: MGI
  • ventricular cardiac muscle cell development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Autophagy, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-MMU-1632852. Macroautophagy.
R-MMU-5205685. Pink/Parkin Mediated Mitophagy.
R-MMU-936440. Negative regulators of RIG-I/MDA5 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy protein 5
Alternative name(s):
APG5-like
Gene namesi
Name:Atg5
Synonyms:Apg5l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1277186. Atg5.

Subcellular locationi

GO - Cellular componenti

  • Atg12-Atg5-Atg16 complex Source: GO_Central
  • autophagosome Source: MGI
  • axoneme Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • ER-mitochondrion membrane contact site Source: MGI
  • membrane Source: MGI
  • pre-autophagosomal structure membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi130K → R: Loss of conjugation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002189951 – 275Autophagy protein 5Add BLAST275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Cross-linki130Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12)By similarity

Post-translational modificationi

Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
Acetylated by EP300.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ99J83.
MaxQBiQ99J83.
PaxDbiQ99J83.
PeptideAtlasiQ99J83.
PRIDEiQ99J83.

PTM databases

iPTMnetiQ99J83.
PhosphoSitePlusiQ99J83.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSMUSG00000038160.
CleanExiMM_ATG5.
GenevisibleiQ99J83. MM.

Interactioni

Subunit structurei

Forms a conjugate with ATG12. The ATG5-ATG12 conjugate forms a complex with several units of ATG16L. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5-ATG12 conjugate (By similarity). ATG12-ATG5 also interacts with MAVS, MGA, and RARRES3 (By similarity). Interacts with ATG3, ATG7 and ATG10. Interacts with FADD (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Atg12Q9CQY14EBI-2911848,EBI-2911788
Atg16l1Q8C0J24EBI-2911848,EBI-769195

Protein-protein interaction databases

BioGridi198146. 7 interactors.
DIPiDIP-46420N.
IntActiQ99J83. 10 interactors.
MINTiMINT-4088049.
STRINGi10090.ENSMUSP00000044769.

Structurei

3D structure databases

ProteinModelPortaliQ99J83.
SMRiQ99J83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG5 family.Curated

Phylogenomic databases

eggNOGiKOG2976. Eukaryota.
ENOG410XQ71. LUCA.
GeneTreeiENSGT00390000004766.
HOGENOMiHOG000007893.
HOVERGENiHBG018731.
InParanoidiQ99J83.
KOiK08339.
OMAiETPIQWL.
OrthoDBiEOG091G0JU7.
PhylomeDBiQ99J83.
TreeFamiTF314415.

Family and domain databases

InterProiIPR007239. Atg5.
[Graphical view]
PANTHERiPTHR13040. PTHR13040. 1 hit.
PfamiPF04106. APG5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99J83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD
60 70 80 90 100
KVKKHFQKVM RQEDVSEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI
110 120 130 140 150
TVHFKSFPEK DLLHCPSKDA VEAHFMSCMK EADALKHKSQ VINEMQKKDH
160 170 180 190 200
KQLWMGLQND RFDQFWAINR KLMEYPPEEN GFRYIPFRIY QTTTERPFIQ
210 220 230 240 250
KLFRPVAADG QLHTLGDLLR EVCPSAVAPE DGEKRSQVMI HGIEPMLETP
260 270
LQWLSEHLSY PDNFLHISIV PQPTD
Length:275
Mass (Da):32,402
Last modified:June 1, 2001 - v1
Checksum:iF3FCE652D627B694
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB048349 mRNA. Translation: BAB33383.1.
AK028315 mRNA. Translation: BAC25874.1.
BC002166 mRNA. Translation: AAH02166.1.
CCDSiCCDS23824.1.
RefSeqiNP_001300942.1. NM_001314013.1.
NP_444299.1. NM_053069.6.
XP_011241410.1. XM_011243108.2.
UniGeneiMm.22264.

Genome annotation databases

EnsembliENSMUST00000039286; ENSMUSP00000044769; ENSMUSG00000038160.
GeneIDi11793.
KEGGimmu:11793.
UCSCiuc007ezt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB048349 mRNA. Translation: BAB33383.1.
AK028315 mRNA. Translation: BAC25874.1.
BC002166 mRNA. Translation: AAH02166.1.
CCDSiCCDS23824.1.
RefSeqiNP_001300942.1. NM_001314013.1.
NP_444299.1. NM_053069.6.
XP_011241410.1. XM_011243108.2.
UniGeneiMm.22264.

3D structure databases

ProteinModelPortaliQ99J83.
SMRiQ99J83.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198146. 7 interactors.
DIPiDIP-46420N.
IntActiQ99J83. 10 interactors.
MINTiMINT-4088049.
STRINGi10090.ENSMUSP00000044769.

PTM databases

iPTMnetiQ99J83.
PhosphoSitePlusiQ99J83.

Proteomic databases

EPDiQ99J83.
MaxQBiQ99J83.
PaxDbiQ99J83.
PeptideAtlasiQ99J83.
PRIDEiQ99J83.

Protocols and materials databases

DNASUi11793.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039286; ENSMUSP00000044769; ENSMUSG00000038160.
GeneIDi11793.
KEGGimmu:11793.
UCSCiuc007ezt.1. mouse.

Organism-specific databases

CTDi9474.
MGIiMGI:1277186. Atg5.

Phylogenomic databases

eggNOGiKOG2976. Eukaryota.
ENOG410XQ71. LUCA.
GeneTreeiENSGT00390000004766.
HOGENOMiHOG000007893.
HOVERGENiHBG018731.
InParanoidiQ99J83.
KOiK08339.
OMAiETPIQWL.
OrthoDBiEOG091G0JU7.
PhylomeDBiQ99J83.
TreeFamiTF314415.

Enzyme and pathway databases

ReactomeiR-MMU-1632852. Macroautophagy.
R-MMU-5205685. Pink/Parkin Mediated Mitophagy.
R-MMU-936440. Negative regulators of RIG-I/MDA5 signaling.

Miscellaneous databases

PROiQ99J83.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038160.
CleanExiMM_ATG5.
GenevisibleiQ99J83. MM.

Family and domain databases

InterProiIPR007239. Atg5.
[Graphical view]
PANTHERiPTHR13040. PTHR13040. 1 hit.
PfamiPF04106. APG5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATG5_MOUSE
AccessioniPrimary (citable) accession number: Q99J83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.