Q99J83 (ATG5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autophagy protein 5

Alternative name(s):
APG5-like

Gene names

Name:	Atg5
Synonyms:	Apg5l

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Ref.1
Subcellular location	Cytoplasm. Note: Cytoplasmic or associated with cytoplasmic isolation membrane.
Tissue specificity	Ubiquitous Probable.
Post-translational modification	Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
Sequence similarities	Belongs to the ATG5 family.

Ontologies

Keywords
Biological process	Autophagy
Cellular component	Cytoplasm
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	autophagic vacuole assembly Inferred from mutant phenotype Ref.1. Source: UniProtKB blood vessel remodeling Inferred from mutant phenotype. Source: MGI heart contraction Inferred from mutant phenotype. Source: MGI negative regulation of apoptotic process Inferred from mutant phenotype. Source: MGI negative regulation of protein ubiquitination Inferred from mutant phenotype. Source: MGI otolith development Inferred from mutant phenotype. Source: MGI post-translational protein modification Inferred from direct assay Ref.1. Source: UniProtKB response to drug Inferred from mutant phenotype. Source: MGI vasodilation Inferred from mutant phenotype. Source: MGI ventricular cardiac muscle cell development Inferred from mutant phenotype. Source: MGI
Cellular component	autophagic vacuole Inferred from direct assay Ref.1. Source: MGI cytosol Traceable author statement. Source: Reactome pre-autophagosomal structure membrane Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Autophagy protein 5		PRO_0000218995
Amino acid modifications
	Cross-link	130		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12) By similarity
Experimental info
	Mutagenesis	130	1	K → R: Loss of conjugation. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99J83 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: F3FCE652D627B694	FASTA	275	32,402
	10 20 30 40 50 60 MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD KVKKHFQKVM 70 80 90 100 110 120 RQEDVSEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI TVHFKSFPEK DLLHCPSKDA 130 140 150 160 170 180 VEAHFMSCMK EADALKHKSQ VINEMQKKDH KQLWMGLQND RFDQFWAINR KLMEYPPEEN 190 200 210 220 230 240 GFRYIPFRIY QTTTERPFIQ KLFRPVAADG QLHTLGDLLR EVCPSAVAPE DGEKRSQVMI 250 260 270 HGIEPMLETP LQWLSEHLSY PDNFLHISIV PQPTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells." Mizushima N., Yamamoto A., Hatano M., Kobayashi Y., Kabeya Y., Suzuki K., Tokuhisa T., Ohsumi Y., Yoshimori T. J. Cell Biol. 152:657-668(2001) [PubMed: 11266458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CONJUGATION TO ATG12, MUTAGENESIS OF LYS-130. Tissue: Embryonic stem cell.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB048349 mRNA. Translation: BAB33383.1. AK028315 mRNA. Translation: BAC25874.1. BC002166 mRNA. Translation: AAH02166.1.
IPI	IPI00114936.
RefSeq	NP_444299.1. NM_053069.5.
UniGene	Mm.22264.
3D structure databases
ProteinModelPortal	Q99J83.
SMR	Q99J83. Positions 4-272.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46420N.
IntAct	Q99J83. 5 interactions.
STRING	Q99J83.
Proteomic databases
PRIDE	Q99J83.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000039286; ENSMUSP00000044769; ENSMUSG00000038160.
GeneID	11793.
KEGG	mmu:11793.
UCSC	uc007ezt.1. mouse.
Organism-specific databases
CTD	9474.
MGI	MGI:1277186. Atg5.
Phylogenomic databases
eggNOG	roNOG06663.
GeneTree	ENSGT00390000004766.
HOGENOM	HBG378773.
HOVERGEN	HBG018731.
InParanoid	Q99J83.
OMA	HFKNFPE.
OrthoDB	EOG4T783Z.
PhylomeDB	Q99J83.
Gene expression databases
ArrayExpress	Q99J83.
Bgee	Q99J83.
CleanEx	MM_ATG5.
Genevestigator	Q99J83.
GermOnline	ENSMUSG00000038160. Mus musculus.
Family and domain databases
InterPro	IPR007239. Autophagy-rel_prot_5. [Graphical view]
KO	K08339.
PANTHER	PTHR13040. APG5. 1 hit.
Pfam	PF04106. APG5. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279625.
SOURCE	Search...

Entry information

Entry name

ATG5_MOUSE

Accession

Primary (citable) accession number: Q99J83

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program