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Protein

Integrin-linked protein kinase

Gene

Ilk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi199 – 2079ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • integrin binding Source: RGD
  • protein domain specific binding Source: RGD
  • protein kinase activity Source: RGD
  • protein serine/threonine kinase activity Source: RGD
  • SH3 domain binding Source: RGD
  • signal transducer activity Source: RGD

GO - Biological processi

  • branching involved in ureteric bud morphogenesis Source: Ensembl
  • cell aging Source: RGD
  • cell cycle arrest Source: RGD
  • establishment or maintenance of epithelial cell apical/basal polarity Source: Ensembl
  • extracellular fibril organization Source: RGD
  • fibroblast migration Source: Ensembl
  • integrin-mediated signaling pathway Source: RGD
  • myelin assembly Source: RGD
  • myelination in peripheral nervous system Source: Ensembl
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of cardiac muscle cell apoptotic process Source: RGD
  • negative regulation of neural precursor cell proliferation Source: Ensembl
  • negative regulation of neuron apoptotic process Source: RGD
  • negative regulation of protein kinase activity Source: RGD
  • negative regulation of smooth muscle cell migration Source: RGD
  • negative regulation of smooth muscle cell proliferation Source: RGD
  • nerve development Source: Ensembl
  • neuron projection morphogenesis Source: RGD
  • outflow tract morphogenesis Source: Ensembl
  • peptidyl-serine phosphorylation Source: RGD
  • platelet aggregation Source: Ensembl
  • positive regulation of axon extension Source: RGD
  • positive regulation of axonogenesis Source: RGD
  • positive regulation of BMP signaling pathway Source: Ensembl
  • positive regulation of canonical Wnt signaling pathway Source: Ensembl
  • positive regulation of cell-matrix adhesion Source: RGD
  • positive regulation of cell migration Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of dendrite morphogenesis Source: RGD
  • positive regulation of MAPK cascade Source: RGD
  • positive regulation of MAP kinase activity Source: RGD
  • positive regulation of myoblast differentiation Source: RGD
  • positive regulation of osteoblast differentiation Source: Ensembl
  • positive regulation of protein kinase B signaling Source: RGD
  • positive regulation of transcription, DNA-templated Source: Ensembl
  • protein heterooligomerization Source: RGD
  • protein kinase B signaling Source: Ensembl
  • protein phosphorylation Source: RGD
  • regulation of actin cytoskeleton organization Source: RGD
  • regulation of cell growth Source: RGD
  • substrate adhesion-dependent cell spreading Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-446343. Localization of the PINCH-ILK-PARVIN complex to focal adhesions.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin-linked protein kinase (EC:2.7.11.1)
Gene namesi
Name:Ilk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620063. Ilk.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • cell-cell junction Source: RGD
  • costamere Source: RGD
  • cytoplasm Source: RGD
  • dendritic shaft Source: RGD
  • extracellular matrix Source: GOC
  • focal adhesion Source: MGI
  • lamellipodium Source: UniProtKB-SubCell
  • neuronal cell body Source: RGD
  • nucleoplasm Source: Ensembl
  • plasma membrane Source: UniProtKB-SubCell
  • protein complex Source: RGD
  • sarcomere Source: UniProtKB-SubCell
  • terminal bouton Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Integrin-linked protein kinasePRO_0000259409Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei186 – 1861PhosphoserineBy similarity
Modified residuei426 – 4261N6-acetyllysineBy similarity

Post-translational modificationi

Autophosphorylated on serine residues.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ99J82.
PRIDEiQ99J82.

PTM databases

iPTMnetiQ99J82.

Expressioni

Gene expression databases

GenevisibleiQ99J82. RN.

Interactioni

Subunit structurei

Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with PARVA, PARVB and probably TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Interacts with FERMT2 (By similarity).By similarity

GO - Molecular functioni

  • integrin binding Source: RGD
  • protein domain specific binding Source: RGD
  • SH3 domain binding Source: RGD

Protein-protein interaction databases

BioGridi251032. 2 interactions.
IntActiQ99J82. 2 interactions.
STRINGi10116.ENSRNOP00000025906.

Structurei

3D structure databases

ProteinModelPortaliQ99J82.
SMRiQ99J82. Positions 2-171, 185-452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati2 – 3029ANK 1Add
BLAST
Repeati31 – 6333ANK 2Add
BLAST
Repeati64 – 9633ANK 3Add
BLAST
Repeati97 – 12933ANK 4Add
BLAST
Repeati130 – 17445ANK 5Add
BLAST
Domaini193 – 446254Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 139107Interaction with LIMS1By similarityAdd
BLAST
Regioni180 – 21233PH-like; mediates interaction with TGFB1I1Add
BLAST

Domaini

A PH-like domain is involved in phosphatidylinositol phosphate binding.By similarity

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0195. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129859.
HOGENOMiHOG000047828.
HOVERGENiHBG002437.
InParanoidiQ99J82.
KOiK06272.
OMAiMLKVRDW.
OrthoDBiEOG7T1RB0.
PhylomeDBiQ99J82.
TreeFamiTF315194.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99J82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV
60 70 80 90 100
EMLIMRGARI NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG
110 120 130 140 150
NVPLHYACFW GQDQVAEDLV ANGALVSICN KYGEMPVDKA KAPLRELLRE
160 170 180 190 200
RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG TLNKHSGIDF KQLNFLAKLN
210 220 230 240 250
ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP RLRIFSHPNV
260 270 280 290 300
LPVLGACQAP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD
310 320 330 340 350
MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM
360 370 380 390 400
YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI
410 420 430 440 450
GMKVALEGLR PTIPPGISPH VCKLMKICMN EDPAKRPKFD MIVPILEKMQ

DK
Length:452
Mass (Da):51,373
Last modified:June 1, 2001 - v1
Checksum:iF41960CF8EC503A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF329194 mRNA. Translation: AAK12419.1.
BC062406 mRNA. Translation: AAH62406.1.
RefSeqiNP_596900.1. NM_133409.2.
UniGeneiRn.95042.

Genome annotation databases

EnsembliENSRNOT00000025906; ENSRNOP00000025906; ENSRNOG00000018993.
GeneIDi170922.
KEGGirno:170922.
UCSCiRGD:620063. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF329194 mRNA. Translation: AAK12419.1.
BC062406 mRNA. Translation: AAH62406.1.
RefSeqiNP_596900.1. NM_133409.2.
UniGeneiRn.95042.

3D structure databases

ProteinModelPortaliQ99J82.
SMRiQ99J82. Positions 2-171, 185-452.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251032. 2 interactions.
IntActiQ99J82. 2 interactions.
STRINGi10116.ENSRNOP00000025906.

PTM databases

iPTMnetiQ99J82.

Proteomic databases

PaxDbiQ99J82.
PRIDEiQ99J82.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025906; ENSRNOP00000025906; ENSRNOG00000018993.
GeneIDi170922.
KEGGirno:170922.
UCSCiRGD:620063. rat.

Organism-specific databases

CTDi3611.
RGDi620063. Ilk.

Phylogenomic databases

eggNOGiKOG0195. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129859.
HOGENOMiHOG000047828.
HOVERGENiHBG002437.
InParanoidiQ99J82.
KOiK06272.
OMAiMLKVRDW.
OrthoDBiEOG7T1RB0.
PhylomeDBiQ99J82.
TreeFamiTF315194.

Enzyme and pathway databases

ReactomeiR-RNO-446343. Localization of the PINCH-ILK-PARVIN complex to focal adhesions.

Miscellaneous databases

PROiQ99J82.

Gene expression databases

GenevisibleiQ99J82. RN.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Integrin-linked kinase (ILK) binding to paxillin LD1 motif regulates ILK localization to focal adhesions."
    Nikolopoulos S.N., Turner C.E.
    J. Biol. Chem. 276:23499-23505(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiILK_RAT
AccessioniPrimary (citable) accession number: Q99J82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.