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Protein

Mucolipin-1

Gene

Mcoln1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cation channel probably playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. Could play a major role in Ca2+ transport regulating lysosomal exocytosis (By similarity).By similarity

Enzyme regulationi

Channel function is transiently modulated by changes in Ca2+, and inhibited by a reduction of pH; pH changes modify the aggregation state of unitary channels.By similarity

GO - Molecular functioni

GO - Biological processi

  • autophagosome maturation Source: MGI
  • endosomal transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.
R-MMU-917977. Transferrin endocytosis and recycling.

Names & Taxonomyi

Protein namesi
Recommended name:
Mucolipin-1
Alternative name(s):
Mucolipidin
Gene namesi
Name:Mcoln1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1890498. Mcoln1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei73 – 9321HelicalSequence analysisAdd
BLAST
Transmembranei297 – 31721HelicalSequence analysisAdd
BLAST
Transmembranei350 – 37021HelicalSequence analysisAdd
BLAST
Transmembranei388 – 40821HelicalSequence analysisAdd
BLAST
Transmembranei428 – 44821HelicalSequence analysisAdd
BLAST
Transmembranei497 – 51721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Mucolipin-1PRO_0000215364Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphoserineCombined sources
Modified residuei557 – 5571PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99J21.
MaxQBiQ99J21.
PaxDbiQ99J21.
PeptideAtlasiQ99J21.
PRIDEiQ99J21.

PTM databases

iPTMnetiQ99J21.
PhosphoSiteiQ99J21.
SwissPalmiQ99J21.

Expressioni

Tissue specificityi

Widely expressed, with the highest expression in brain, liver and kidney.1 Publication

Gene expression databases

BgeeiQ99J21.
CleanExiMM_MCOLN1.
ExpressionAtlasiQ99J21. baseline and differential.
GenevisibleiQ99J21. MM.

Interactioni

Subunit structurei

Forms multimeric complexes. Interacts with PDCD6 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004683.

Structurei

3D structure databases

ProteinModelPortaliQ99J21.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3733. Eukaryota.
ENOG410Z1HH. LUCA.
GeneTreeiENSGT00390000017126.
HOGENOMiHOG000232158.
HOVERGENiHBG052430.
InParanoidiQ99J21.
KOiK04992.
OMAiPANDTFN.
OrthoDBiEOG7V1FQ9.
PhylomeDBiQ99J21.
TreeFamiTF317783.

Family and domain databases

InterProiIPR013122. PKD1_2_channel.
[Graphical view]
PfamiPF08016. PKD_channel. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99J21-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATPAGRRAS ETERLLTPNP GYGTQVGTSP APTTPTEEED LRRRLKYFFM
60 70 80 90 100
SPCDKFRAKG RKPCKLMLQV VKILVVTVQL ILFGLSNQLV VTFREENTIA
110 120 130 140 150
FRHLFLLGYS DGSDDTFAAY TQEQLYQAIF YAVDQYLILP EISLGRYAYV
160 170 180 190 200
RGGGGPWANG SALALCQRYY HRGHVDPAND TFDIDPRVVT DCIQVDPPDR
210 220 230 240 250
PPDIPSEDLD FLDGSASYKN LTLKFHKLIN VTIHFQLKTI NLQSLINNEI
260 270 280 290 300
PDCYTFSILI TFDNKAHSGR IPIRLETKTH IQECKHPSVS RHGDNSFRLL
310 320 330 340 350
FDVVVILTCS LSFLLCARSL LRGFLLQNEF VVFMWRRRGR EISLWERLEF
360 370 380 390 400
VNGWYILLVT SDVLTISGTV MKIGIEAKNL ASYDVCSILL GTSTLLVWVG
410 420 430 440 450
VIRYLTFFHK YNILIATLRV ALPSVMRFCC CVAVIYLGYC FCGWIVLGPY
460 470 480 490 500
HVKFRSLSMV SECLFSLING DDMFVTFAAM QAQQGHSSLV WLFSQLYLYS
510 520 530 540 550
FISLFIYMVL SLFIALITGA YDTIKHPGGT GTEKSELQAY IEQCQDSPTS
560 570 580
GKFRRGSGSA CSLFCCCGRD SPEDHSLLVN
Length:580
Mass (Da):65,506
Last modified:June 1, 2001 - v1
Checksum:iF64D2E6C5D4C041C
GO
Isoform 2 (identifier: Q99J21-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     526-580: HPGGTGTEKS...SPEDHSLLVN → RTNHRNWFSS...SSSTMWSHQV

Show »
Length:611
Mass (Da):69,853
Checksum:iC7E67D1C1DAD96AD
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei526 – 58055HPGGT…SLLVN → RTNHRNWFSSFTVWIIDLNT SYQLCSKCLYLPHHLTGFLP LSYHLLLKTIYLFRGERRLC TAQCVWKLETTCGNQLSSST MWSHQV in isoform 2. 1 PublicationVSP_010821Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302009 mRNA. Translation: AAL58667.1.
AK028385 mRNA. Translation: BAC25922.1.
AK034471 mRNA. Translation: BAC28719.1.
AK049606 mRNA. Translation: BAC33838.1.
BC005651 mRNA. Translation: AAH05651.1.
CCDSiCCDS22063.1. [Q99J21-1]
RefSeqiNP_444407.1. NM_053177.1. [Q99J21-1]
UniGeneiMm.8356.

Genome annotation databases

EnsembliENSMUST00000004683; ENSMUSP00000004683; ENSMUSG00000004567. [Q99J21-1]
GeneIDi94178.
KEGGimmu:94178.
UCSCiuc009krq.1. mouse. [Q99J21-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302009 mRNA. Translation: AAL58667.1.
AK028385 mRNA. Translation: BAC25922.1.
AK034471 mRNA. Translation: BAC28719.1.
AK049606 mRNA. Translation: BAC33838.1.
BC005651 mRNA. Translation: AAH05651.1.
CCDSiCCDS22063.1. [Q99J21-1]
RefSeqiNP_444407.1. NM_053177.1. [Q99J21-1]
UniGeneiMm.8356.

3D structure databases

ProteinModelPortaliQ99J21.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004683.

PTM databases

iPTMnetiQ99J21.
PhosphoSiteiQ99J21.
SwissPalmiQ99J21.

Proteomic databases

EPDiQ99J21.
MaxQBiQ99J21.
PaxDbiQ99J21.
PeptideAtlasiQ99J21.
PRIDEiQ99J21.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004683; ENSMUSP00000004683; ENSMUSG00000004567. [Q99J21-1]
GeneIDi94178.
KEGGimmu:94178.
UCSCiuc009krq.1. mouse. [Q99J21-1]

Organism-specific databases

CTDi57192.
MGIiMGI:1890498. Mcoln1.

Phylogenomic databases

eggNOGiKOG3733. Eukaryota.
ENOG410Z1HH. LUCA.
GeneTreeiENSGT00390000017126.
HOGENOMiHOG000232158.
HOVERGENiHBG052430.
InParanoidiQ99J21.
KOiK04992.
OMAiPANDTFN.
OrthoDBiEOG7V1FQ9.
PhylomeDBiQ99J21.
TreeFamiTF317783.

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.
R-MMU-917977. Transferrin endocytosis and recycling.

Miscellaneous databases

PROiQ99J21.
SOURCEiSearch...

Gene expression databases

BgeeiQ99J21.
CleanExiMM_MCOLN1.
ExpressionAtlasiQ99J21. baseline and differential.
GenevisibleiQ99J21. MM.

Family and domain databases

InterProiIPR013122. PKD1_2_channel.
[Graphical view]
PfamiPF08016. PKD_channel. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the mouse Mcoln1 gene reveals an alternatively spliced transcript not seen in humans."
    Falardeau J.L., Kennedy J.C., Acierno J.S. Jr., Sun M., Stahl S., Goldin E., Slaugenhaupt S.A.
    BMC Genomics 3:3-3(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Diencephalon, Extraembryonic tissue, Placenta and Spinal cord.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Mammary tumor.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  5. "Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS."
    Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y.
    Mol. Cell. Proteomics 6:669-676(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-557, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Liver and Testis.

Entry informationi

Entry nameiMCLN1_MOUSE
AccessioniPrimary (citable) accession number: Q99J21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.