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Protein

Cytoplasmic tRNA 2-thiolation protein 1

Gene

Ctu1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.UniRule annotation

Pathwayi: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis

This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processtRNA processing

Enzyme and pathway databases

UniPathwayiUPA00988

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic tRNA 2-thiolation protein 1UniRule annotation (EC:2.7.7.-UniRule annotation)
Alternative name(s):
ATP-binding domain-containing protein 3UniRule annotation
Cytoplasmic tRNA adenylyltransferase 1UniRule annotation
Gene namesi
Name:Ctu1
Synonyms:Atpbd3, Ncs6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2385277 Ctu1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002823921 – 420Cytoplasmic tRNA 2-thiolation protein 1Add BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei200PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99J10
MaxQBiQ99J10
PaxDbiQ99J10
PeptideAtlasiQ99J10
PRIDEiQ99J10

PTM databases

iPTMnetiQ99J10
PhosphoSitePlusiQ99J10

Expressioni

Gene expression databases

BgeeiENSMUSG00000038888
CleanExiMM_ATPBD3
GenevisibleiQ99J10 MM

Interactioni

Subunit structurei

Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3. May form a heterodimer with CTU2/NCS2.UniRule annotation

Protein-protein interaction databases

BioGridi231384, 2 interactors
STRINGi10090.ENSMUSP00000036770

Structurei

3D structure databases

ProteinModelPortaliQ99J10
SMRiQ99J10
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2840 Eukaryota
COG0037 LUCA
GeneTreeiENSGT00390000001041
HOGENOMiHOG000225864
HOVERGENiHBG100428
InParanoidiQ99J10
KOiK14168
OMAiKPLKYSY
OrthoDBiEOG091G0CF7
PhylomeDBiQ99J10
TreeFamiTF352405

Family and domain databases

Gene3Di3.40.50.620, 1 hit
HAMAPiMF_03053 CTU1, 1 hit
InterProiView protein in InterPro
IPR032442 CTU1_C
IPR000541 Ncs6/Tuc1/Ctu1
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
PANTHERiPTHR11807:SF12 PTHR11807:SF12, 1 hit
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
PF16503 zn-ribbon_14, 1 hit

Sequencei

Sequence statusi: Complete.

Q99J10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAPTCFSCH KTRAALRRPR SGQALCGSCF CAAFEAEVLH TVLAGHLLPP
60 70 80 90 100
GAVVAVGASG GKDSTVLAHV LRELAPRLGI TLHLVAVDEG IGGYRDAALE
110 120 130 140 150
AVRSQAARWE LPLTIVAYED LFGGWTMDAV ARSTAGSGRS RSCCTFCGVL
160 170 180 190 200
RRRALEEGAR LVGATHIVTG HNADDMAETV LMNFLRGDAG RLARGGVLGS
210 220 230 240 250
TGEGCALPRC RPLQFASQKE VVLYAHFRHL RYFSEECVYA PEAFRGHARD
260 270 280 290 300
LLKLLEAARP SAVLDLVHSA ERLALAPAAK PPPPGTCSRC GALASHKLCQ
310 320 330 340 350
ACALLDGLNR GLPRLAIGKG RRVLQVEPPQ PGNPSLVTSD PVAPAGPCTC
360 370 380 390 400
KQPKDKANPC GNGGDRAGAT CVSQCDLSPG NGEDRAGATC VSQRDLSLGN
410 420
GGDRAGATCV SQCDLSPVAE
Length:420
Mass (Da):43,823
Last modified:June 1, 2001 - v1
Checksum:i3DDCEDBE70D196B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK155284 mRNA Translation: BAE33164.1
BC005752 mRNA Translation: AAH05752.1
CCDSiCCDS21179.1
RefSeqiNP_663557.1, NM_145582.1
UniGeneiMm.26514

Genome annotation databases

EnsembliENSMUST00000038332; ENSMUSP00000036770; ENSMUSG00000038888
GeneIDi233189
KEGGimmu:233189
UCSCiuc009gnh.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCTU1_MOUSE
AccessioniPrimary (citable) accession number: Q99J10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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