Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q99J10

- CTU1_MOUSE

UniProt

Q99J10 - CTU1_MOUSE

Protein

Cytoplasmic tRNA 2-thiolation protein 1

Gene

Ctu1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.UniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. nucleotidyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB

    GO - Biological processi

    1. protein urmylation Source: UniProtKB-HAMAP
    2. tRNA thio-modification Source: UniProtKB
    3. tRNA wobble uridine modification Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00988.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytoplasmic tRNA 2-thiolation protein 1UniRule annotation (EC:2.7.7.-UniRule annotation)
    Alternative name(s):
    ATP-binding domain-containing protein 3UniRule annotation
    Cytoplasmic tRNA adenylyltransferase 1UniRule annotation
    Gene namesi
    Name:Ctu1
    Synonyms:Atpbd3, Ncs6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:2385277. Ctu1.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 420420Cytoplasmic tRNA 2-thiolation protein 1PRO_0000282392Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei200 – 2001PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ99J10.
    PRIDEiQ99J10.

    PTM databases

    PhosphoSiteiQ99J10.

    Expressioni

    Gene expression databases

    BgeeiQ99J10.
    CleanExiMM_ATPBD3.
    GenevestigatoriQ99J10.

    Interactioni

    Subunit structurei

    Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3. May form a heterodimer with CTU2/NCS2.UniRule annotation

    Protein-protein interaction databases

    BioGridi231384. 1 interaction.
    STRINGi10090.ENSMUSP00000036770.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99J10.
    SMRiQ99J10. Positions 54-236.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0037.
    GeneTreeiENSGT00390000001041.
    HOGENOMiHOG000225864.
    HOVERGENiHBG100428.
    InParanoidiQ99J10.
    KOiK14168.
    OMAiICTQGED.
    OrthoDBiEOG7PGDRH.
    PhylomeDBiQ99J10.
    TreeFamiTF352405.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_03053. CTU1.
    InterProiIPR000541. Ncs6/Tuc1/Ctu1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR011063. tRNA-lysidine/thiocyt_synth.
    [Graphical view]
    PfamiPF01171. ATP_bind_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99J10-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAPTCFSCH KTRAALRRPR SGQALCGSCF CAAFEAEVLH TVLAGHLLPP    50
    GAVVAVGASG GKDSTVLAHV LRELAPRLGI TLHLVAVDEG IGGYRDAALE 100
    AVRSQAARWE LPLTIVAYED LFGGWTMDAV ARSTAGSGRS RSCCTFCGVL 150
    RRRALEEGAR LVGATHIVTG HNADDMAETV LMNFLRGDAG RLARGGVLGS 200
    TGEGCALPRC RPLQFASQKE VVLYAHFRHL RYFSEECVYA PEAFRGHARD 250
    LLKLLEAARP SAVLDLVHSA ERLALAPAAK PPPPGTCSRC GALASHKLCQ 300
    ACALLDGLNR GLPRLAIGKG RRVLQVEPPQ PGNPSLVTSD PVAPAGPCTC 350
    KQPKDKANPC GNGGDRAGAT CVSQCDLSPG NGEDRAGATC VSQRDLSLGN 400
    GGDRAGATCV SQCDLSPVAE 420
    Length:420
    Mass (Da):43,823
    Last modified:June 1, 2001 - v1
    Checksum:i3DDCEDBE70D196B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK155284 mRNA. Translation: BAE33164.1.
    BC005752 mRNA. Translation: AAH05752.1.
    CCDSiCCDS21179.1.
    RefSeqiNP_663557.1. NM_145582.1.
    UniGeneiMm.26514.

    Genome annotation databases

    EnsembliENSMUST00000038332; ENSMUSP00000036770; ENSMUSG00000038888.
    GeneIDi233189.
    KEGGimmu:233189.
    UCSCiuc009gnh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK155284 mRNA. Translation: BAE33164.1 .
    BC005752 mRNA. Translation: AAH05752.1 .
    CCDSi CCDS21179.1.
    RefSeqi NP_663557.1. NM_145582.1.
    UniGenei Mm.26514.

    3D structure databases

    ProteinModelPortali Q99J10.
    SMRi Q99J10. Positions 54-236.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 231384. 1 interaction.
    STRINGi 10090.ENSMUSP00000036770.

    PTM databases

    PhosphoSitei Q99J10.

    Proteomic databases

    PaxDbi Q99J10.
    PRIDEi Q99J10.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000038332 ; ENSMUSP00000036770 ; ENSMUSG00000038888 .
    GeneIDi 233189.
    KEGGi mmu:233189.
    UCSCi uc009gnh.1. mouse.

    Organism-specific databases

    CTDi 90353.
    MGIi MGI:2385277. Ctu1.

    Phylogenomic databases

    eggNOGi COG0037.
    GeneTreei ENSGT00390000001041.
    HOGENOMi HOG000225864.
    HOVERGENi HBG100428.
    InParanoidi Q99J10.
    KOi K14168.
    OMAi ICTQGED.
    OrthoDBi EOG7PGDRH.
    PhylomeDBi Q99J10.
    TreeFami TF352405.

    Enzyme and pathway databases

    UniPathwayi UPA00988 .

    Miscellaneous databases

    NextBioi 381599.
    PROi Q99J10.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q99J10.
    CleanExi MM_ATPBD3.
    Genevestigatori Q99J10.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_03053. CTU1.
    InterProi IPR000541. Ncs6/Tuc1/Ctu1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR011063. tRNA-lysidine/thiocyt_synth.
    [Graphical view ]
    Pfami PF01171. ATP_bind_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NOD.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NMRI.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiCTU1_MOUSE
    AccessioniPrimary (citable) accession number: Q99J10
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3