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Q99J09

- MEP50_MOUSE

UniProt

Q99J09 - MEP50_MOUSE

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Protein
Methylosome protein 50
Gene
Wdr77, Mep50
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation By similarity. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.1 Publication

GO - Molecular functioni

  1. ligand-dependent nuclear receptor transcription coactivator activity Source: Ensembl
  2. protein binding Source: MGI
Complete GO annotation...

GO - Biological processi

  1. negative regulation of cell proliferation Source: MGI
  2. negative regulation of epithelial cell proliferation involved in prostate gland development Source: MGI
  3. positive regulation of cell proliferation Source: MGI
  4. regulation of transcription from RNA polymerase II promoter Source: MGI
  5. secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_206812. snRNP Assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome protein 50
Short name:
MEP-50
Alternative name(s):
WD repeat-containing protein 77
Gene namesi
Name:Wdr77
Synonyms:Mep50
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1917715. Wdr77.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity

GO - Cellular componenti

  1. Golgi apparatus Source: Ensembl
  2. cytoplasm Source: UniProtKB
  3. cytosol Source: UniProtKB
  4. methylosome Source: UniProtKB
  5. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Methylosome protein 50
PRO_0000051075Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51Phosphothreonine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99J09.
PaxDbiQ99J09.
PRIDEiQ99J09.

PTM databases

PhosphoSiteiQ99J09.

Expressioni

Gene expression databases

ArrayExpressiQ99J09.
BgeeiQ99J09.
CleanExiMM_WDR77.
GenevestigatoriQ99J09.

Interactioni

Subunit structurei

Component of the methylosome, a 20S complex containing at least PRMT5, CLNS1A and WDR77. Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4. Interacts with CTDP1 and LSM11. Interacts with APEX1, AR and NKX3-1 By similarity.1 Publication

Protein-protein interaction databases

IntActiQ99J09. 4 interactions.
MINTiMINT-4101776.

Structurei

3D structure databases

ProteinModelPortaliQ99J09.
SMRiQ99J09. Positions 21-328.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati22 – 7554WD 1
Add
BLAST
Repeati78 – 11639WD 2
Add
BLAST
Repeati123 – 16240WD 3
Add
BLAST
Repeati165 – 20541WD 4
Add
BLAST
Repeati209 – 25042WD 5
Add
BLAST
Repeati253 – 29341WD 6
Add
BLAST
Repeati295 – 33036WD 7
Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00390000010711.
HOGENOMiHOG000035094.
HOVERGENiHBG052458.
InParanoidiQ99J09.
KOiK13221.
OMAiMRKETPP.
OrthoDBiEOG7KSX99.
PhylomeDBiQ99J09.
TreeFamiTF325967.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99J09-1 [UniParc]FASTAAdd to Basket

« Hide

MRKDTPPPLV PPAAREWNLP PNAPACMERQ LEAARYRSDG SLLLGVSSLS    50
GRCWVGSLWF FKDPSAAPNE GFCSAGVQTE AGVADLTWVG DKGILVASDS 100
GAVELWELDE NETLIVSKFC KYEHDDIVST VTVLSSGTQA VSGSKDCCIK 150
IWDLAQQVSL NSYRAHAGQV TCVAASPHKD SVFLSCSEDS RILLWDTRCP 200
KPASQMACNA SGYLPTALAW HPQQSEVFVF GDENGSVSLV DTKNASCTLS 250
SAVHSQGVTR LVFSPHSVPL LTSLSEDCSL AVLDSSLSEV FRSRAHRDFV 300
RDATWSPLNH SLLTTVGWDH QVIHHVVPLE PLPNPGPDSV VE 342
Length:342
Mass (Da):36,943
Last modified:June 1, 2001 - v1
Checksum:iE9C52BC4D6E5AC36
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti267 – 28519SVPLL…AVLDS → RCCVSPGTWKGWVGTVVKE in AAH05755. 1 Publication
Add
BLAST
Sequence conflicti286 – 34257Missing in AAH05755. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK012014 mRNA. Translation: BAB27975.1.
AK032897 mRNA. Translation: BAC28076.1.
AK048488 mRNA. Translation: BAC33350.1.
AK050391 mRNA. Translation: BAC34232.1.
AK163839 mRNA. Translation: BAE37512.1.
AK169128 mRNA. Translation: BAE40907.1.
BC005755 mRNA. Translation: AAH05755.1.
CCDSiCCDS17715.1.
RefSeqiNP_081708.1. NM_027432.3.
UniGeneiMm.5110.

Genome annotation databases

EnsembliENSMUST00000010278; ENSMUSP00000010278; ENSMUSG00000000561.
GeneIDi70465.
KEGGimmu:70465.
UCSCiuc008qvo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK012014 mRNA. Translation: BAB27975.1 .
AK032897 mRNA. Translation: BAC28076.1 .
AK048488 mRNA. Translation: BAC33350.1 .
AK050391 mRNA. Translation: BAC34232.1 .
AK163839 mRNA. Translation: BAE37512.1 .
AK169128 mRNA. Translation: BAE40907.1 .
BC005755 mRNA. Translation: AAH05755.1 .
CCDSi CCDS17715.1.
RefSeqi NP_081708.1. NM_027432.3.
UniGenei Mm.5110.

3D structure databases

ProteinModelPortali Q99J09.
SMRi Q99J09. Positions 21-328.
ModBasei Search...

Protein-protein interaction databases

IntActi Q99J09. 4 interactions.
MINTi MINT-4101776.

PTM databases

PhosphoSitei Q99J09.

Proteomic databases

MaxQBi Q99J09.
PaxDbi Q99J09.
PRIDEi Q99J09.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000010278 ; ENSMUSP00000010278 ; ENSMUSG00000000561 .
GeneIDi 70465.
KEGGi mmu:70465.
UCSCi uc008qvo.1. mouse.

Organism-specific databases

CTDi 79084.
MGIi MGI:1917715. Wdr77.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00390000010711.
HOGENOMi HOG000035094.
HOVERGENi HBG052458.
InParanoidi Q99J09.
KOi K13221.
OMAi MRKETPP.
OrthoDBi EOG7KSX99.
PhylomeDBi Q99J09.
TreeFami TF325967.

Enzyme and pathway databases

Reactomei REACT_206812. snRNP Assembly.

Miscellaneous databases

ChiTaRSi WDR77. mouse.
NextBioi 331685.
PROi Q99J09.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q99J09.
Bgeei Q99J09.
CleanExi MM_WDR77.
Genevestigatori Q99J09.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 2 hits.
[Graphical view ]
SMARTi SM00320. WD40. 6 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Amnion, Embryo, Head, Liver and Mesonephros.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  3. "Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro."
    Furuno K., Masatsugu T., Sonoda M., Sasazuki T., Yamamoto K.
    Biochem. Biophys. Res. Commun. 345:1051-1058(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SUZ12.
  4. "Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members."
    Vagin V.V., Wohlschlegel J., Qu J., Jonsson Z., Huang X., Chuma S., Girard A., Sachidanandam R., Hannon G.J., Aravin A.A.
    Genes Dev. 23:1749-1762(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN METHYLATION OF PIWI PROTEINS.

Entry informationi

Entry nameiMEP50_MOUSE
AccessioniPrimary (citable) accession number: Q99J09
Secondary accession number(s): Q3TFJ1, Q8BSH8, Q9CZY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi