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Q99J09

- MEP50_MOUSE

UniProt

Q99J09 - MEP50_MOUSE

Protein

Methylosome protein 50

Gene

Wdr77

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation By similarity. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.By similarity1 Publication

    GO - Molecular functioni

    1. ligand-dependent nuclear receptor transcription coactivator activity Source: Ensembl
    2. protein binding Source: MGI

    GO - Biological processi

    1. negative regulation of cell proliferation Source: MGI
    2. negative regulation of epithelial cell proliferation involved in prostate gland development Source: MGI
    3. positive regulation of cell proliferation Source: MGI
    4. regulation of transcription from RNA polymerase II promoter Source: MGI
    5. secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_206812. snRNP Assembly.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylosome protein 50
    Short name:
    MEP-50
    Alternative name(s):
    WD repeat-containing protein 77
    Gene namesi
    Name:Wdr77
    Synonyms:Mep50
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1917715. Wdr77.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytosol Source: UniProtKB
    3. Golgi apparatus Source: Ensembl
    4. methylosome Source: UniProtKB
    5. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 342342Methylosome protein 50PRO_0000051075Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei5 – 51PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ99J09.
    PaxDbiQ99J09.
    PRIDEiQ99J09.

    PTM databases

    PhosphoSiteiQ99J09.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99J09.
    BgeeiQ99J09.
    CleanExiMM_WDR77.
    GenevestigatoriQ99J09.

    Interactioni

    Subunit structurei

    Component of the methylosome, a 20S complex containing at least PRMT5, CLNS1A and WDR77. Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4. Interacts with CTDP1 and LSM11. Interacts with APEX1, AR and NKX3-1 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ99J09. 4 interactions.
    MINTiMINT-4101776.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99J09.
    SMRiQ99J09. Positions 21-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati22 – 7554WD 1Add
    BLAST
    Repeati78 – 11639WD 2Add
    BLAST
    Repeati123 – 16240WD 3Add
    BLAST
    Repeati165 – 20541WD 4Add
    BLAST
    Repeati209 – 25042WD 5Add
    BLAST
    Repeati253 – 29341WD 6Add
    BLAST
    Repeati295 – 33036WD 7Add
    BLAST

    Sequence similaritiesi

    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00390000010711.
    HOGENOMiHOG000035094.
    HOVERGENiHBG052458.
    InParanoidiQ99J09.
    KOiK13221.
    OMAiMRKETPP.
    OrthoDBiEOG7KSX99.
    PhylomeDBiQ99J09.
    TreeFamiTF325967.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 2 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 6 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99J09-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKDTPPPLV PPAAREWNLP PNAPACMERQ LEAARYRSDG SLLLGVSSLS    50
    GRCWVGSLWF FKDPSAAPNE GFCSAGVQTE AGVADLTWVG DKGILVASDS 100
    GAVELWELDE NETLIVSKFC KYEHDDIVST VTVLSSGTQA VSGSKDCCIK 150
    IWDLAQQVSL NSYRAHAGQV TCVAASPHKD SVFLSCSEDS RILLWDTRCP 200
    KPASQMACNA SGYLPTALAW HPQQSEVFVF GDENGSVSLV DTKNASCTLS 250
    SAVHSQGVTR LVFSPHSVPL LTSLSEDCSL AVLDSSLSEV FRSRAHRDFV 300
    RDATWSPLNH SLLTTVGWDH QVIHHVVPLE PLPNPGPDSV VE 342
    Length:342
    Mass (Da):36,943
    Last modified:June 1, 2001 - v1
    Checksum:iE9C52BC4D6E5AC36
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti267 – 28519SVPLL…AVLDS → RCCVSPGTWKGWVGTVVKE in AAH05755. (PubMed:15489334)CuratedAdd
    BLAST
    Sequence conflicti286 – 34257Missing in AAH05755. (PubMed:15489334)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK012014 mRNA. Translation: BAB27975.1.
    AK032897 mRNA. Translation: BAC28076.1.
    AK048488 mRNA. Translation: BAC33350.1.
    AK050391 mRNA. Translation: BAC34232.1.
    AK163839 mRNA. Translation: BAE37512.1.
    AK169128 mRNA. Translation: BAE40907.1.
    BC005755 mRNA. Translation: AAH05755.1.
    CCDSiCCDS17715.1.
    RefSeqiNP_081708.1. NM_027432.3.
    UniGeneiMm.5110.

    Genome annotation databases

    EnsembliENSMUST00000010278; ENSMUSP00000010278; ENSMUSG00000000561.
    GeneIDi70465.
    KEGGimmu:70465.
    UCSCiuc008qvo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK012014 mRNA. Translation: BAB27975.1 .
    AK032897 mRNA. Translation: BAC28076.1 .
    AK048488 mRNA. Translation: BAC33350.1 .
    AK050391 mRNA. Translation: BAC34232.1 .
    AK163839 mRNA. Translation: BAE37512.1 .
    AK169128 mRNA. Translation: BAE40907.1 .
    BC005755 mRNA. Translation: AAH05755.1 .
    CCDSi CCDS17715.1.
    RefSeqi NP_081708.1. NM_027432.3.
    UniGenei Mm.5110.

    3D structure databases

    ProteinModelPortali Q99J09.
    SMRi Q99J09. Positions 21-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q99J09. 4 interactions.
    MINTi MINT-4101776.

    PTM databases

    PhosphoSitei Q99J09.

    Proteomic databases

    MaxQBi Q99J09.
    PaxDbi Q99J09.
    PRIDEi Q99J09.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000010278 ; ENSMUSP00000010278 ; ENSMUSG00000000561 .
    GeneIDi 70465.
    KEGGi mmu:70465.
    UCSCi uc008qvo.1. mouse.

    Organism-specific databases

    CTDi 79084.
    MGIi MGI:1917715. Wdr77.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00390000010711.
    HOGENOMi HOG000035094.
    HOVERGENi HBG052458.
    InParanoidi Q99J09.
    KOi K13221.
    OMAi MRKETPP.
    OrthoDBi EOG7KSX99.
    PhylomeDBi Q99J09.
    TreeFami TF325967.

    Enzyme and pathway databases

    Reactomei REACT_206812. snRNP Assembly.

    Miscellaneous databases

    ChiTaRSi WDR77. mouse.
    NextBioi 331685.
    PROi Q99J09.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99J09.
    Bgeei Q99J09.
    CleanExi MM_WDR77.
    Genevestigatori Q99J09.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 2 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 6 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Amnion, Embryo, Head, Liver and Mesonephros.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    3. "Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro."
      Furuno K., Masatsugu T., Sonoda M., Sasazuki T., Yamamoto K.
      Biochem. Biophys. Res. Commun. 345:1051-1058(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SUZ12.
    4. "Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members."
      Vagin V.V., Wohlschlegel J., Qu J., Jonsson Z., Huang X., Chuma S., Girard A., Sachidanandam R., Hannon G.J., Aravin A.A.
      Genes Dev. 23:1749-1762(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN METHYLATION OF PIWI PROTEINS.

    Entry informationi

    Entry nameiMEP50_MOUSE
    AccessioniPrimary (citable) accession number: Q99J09
    Secondary accession number(s): Q3TFJ1, Q8BSH8, Q9CZY5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2003
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3