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Protein

Methylosome protein 50

Gene

Wdr77

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation (By similarity). The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • negative regulation of cell proliferation Source: MGI
  • negative regulation of epithelial cell proliferation involved in prostate gland development Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_319868. RMTs methylate histone arginines.
REACT_333207. snRNP Assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome protein 50
Short name:
MEP-50
Alternative name(s):
WD repeat-containing protein 77
Gene namesi
Name:Wdr77
Synonyms:Mep50
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1917715. Wdr77.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Methylosome protein 50PRO_0000051075Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99J09.
PaxDbiQ99J09.
PRIDEiQ99J09.

PTM databases

PhosphoSiteiQ99J09.

Expressioni

Gene expression databases

BgeeiQ99J09.
CleanExiMM_WDR77.
ExpressionAtlasiQ99J09. baseline and differential.
GenevisibleiQ99J09. MM.

Interactioni

Subunit structurei

Component of the methylosome, a 20S complex containing at least PRMT5, CLNS1A and WDR77. Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4. Interacts with CTDP1 and LSM11. Interacts with APEX1, AR and NKX3-1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ99J09. 4 interactions.
MINTiMINT-4101776.
STRINGi10090.ENSMUSP00000010278.

Structurei

3D structure databases

ProteinModelPortaliQ99J09.
SMRiQ99J09. Positions 21-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati22 – 7554WD 1Add
BLAST
Repeati78 – 11639WD 2Add
BLAST
Repeati123 – 16240WD 3Add
BLAST
Repeati165 – 20541WD 4Add
BLAST
Repeati209 – 25042WD 5Add
BLAST
Repeati253 – 29341WD 6Add
BLAST
Repeati295 – 33036WD 7Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00390000010711.
HOGENOMiHOG000035094.
HOVERGENiHBG052458.
InParanoidiQ99J09.
KOiK13221.
OMAiWDLAQQV.
OrthoDBiEOG7KSX99.
PhylomeDBiQ99J09.
TreeFamiTF325967.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99J09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKDTPPPLV PPAAREWNLP PNAPACMERQ LEAARYRSDG SLLLGVSSLS
60 70 80 90 100
GRCWVGSLWF FKDPSAAPNE GFCSAGVQTE AGVADLTWVG DKGILVASDS
110 120 130 140 150
GAVELWELDE NETLIVSKFC KYEHDDIVST VTVLSSGTQA VSGSKDCCIK
160 170 180 190 200
IWDLAQQVSL NSYRAHAGQV TCVAASPHKD SVFLSCSEDS RILLWDTRCP
210 220 230 240 250
KPASQMACNA SGYLPTALAW HPQQSEVFVF GDENGSVSLV DTKNASCTLS
260 270 280 290 300
SAVHSQGVTR LVFSPHSVPL LTSLSEDCSL AVLDSSLSEV FRSRAHRDFV
310 320 330 340
RDATWSPLNH SLLTTVGWDH QVIHHVVPLE PLPNPGPDSV VE
Length:342
Mass (Da):36,943
Last modified:June 1, 2001 - v1
Checksum:iE9C52BC4D6E5AC36
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti267 – 28519SVPLL…AVLDS → RCCVSPGTWKGWVGTVVKE in AAH05755 (PubMed:15489334).CuratedAdd
BLAST
Sequence conflicti286 – 34257Missing in AAH05755 (PubMed:15489334).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012014 mRNA. Translation: BAB27975.1.
AK032897 mRNA. Translation: BAC28076.1.
AK048488 mRNA. Translation: BAC33350.1.
AK050391 mRNA. Translation: BAC34232.1.
AK163839 mRNA. Translation: BAE37512.1.
AK169128 mRNA. Translation: BAE40907.1.
BC005755 mRNA. Translation: AAH05755.1.
CCDSiCCDS17715.1.
RefSeqiNP_081708.1. NM_027432.3.
UniGeneiMm.5110.

Genome annotation databases

EnsembliENSMUST00000010278; ENSMUSP00000010278; ENSMUSG00000000561.
GeneIDi70465.
KEGGimmu:70465.
UCSCiuc008qvo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012014 mRNA. Translation: BAB27975.1.
AK032897 mRNA. Translation: BAC28076.1.
AK048488 mRNA. Translation: BAC33350.1.
AK050391 mRNA. Translation: BAC34232.1.
AK163839 mRNA. Translation: BAE37512.1.
AK169128 mRNA. Translation: BAE40907.1.
BC005755 mRNA. Translation: AAH05755.1.
CCDSiCCDS17715.1.
RefSeqiNP_081708.1. NM_027432.3.
UniGeneiMm.5110.

3D structure databases

ProteinModelPortaliQ99J09.
SMRiQ99J09. Positions 21-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99J09. 4 interactions.
MINTiMINT-4101776.
STRINGi10090.ENSMUSP00000010278.

PTM databases

PhosphoSiteiQ99J09.

Proteomic databases

MaxQBiQ99J09.
PaxDbiQ99J09.
PRIDEiQ99J09.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010278; ENSMUSP00000010278; ENSMUSG00000000561.
GeneIDi70465.
KEGGimmu:70465.
UCSCiuc008qvo.1. mouse.

Organism-specific databases

CTDi79084.
MGIiMGI:1917715. Wdr77.

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00390000010711.
HOGENOMiHOG000035094.
HOVERGENiHBG052458.
InParanoidiQ99J09.
KOiK13221.
OMAiWDLAQQV.
OrthoDBiEOG7KSX99.
PhylomeDBiQ99J09.
TreeFamiTF325967.

Enzyme and pathway databases

ReactomeiREACT_319868. RMTs methylate histone arginines.
REACT_333207. snRNP Assembly.

Miscellaneous databases

ChiTaRSiWdr77. mouse.
NextBioi331685.
PROiQ99J09.
SOURCEiSearch...

Gene expression databases

BgeeiQ99J09.
CleanExiMM_WDR77.
ExpressionAtlasiQ99J09. baseline and differential.
GenevisibleiQ99J09. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Amnion, Embryo, Head, Liver and Mesonephros.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  3. "Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro."
    Furuno K., Masatsugu T., Sonoda M., Sasazuki T., Yamamoto K.
    Biochem. Biophys. Res. Commun. 345:1051-1058(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SUZ12.
  4. "Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members."
    Vagin V.V., Wohlschlegel J., Qu J., Jonsson Z., Huang X., Chuma S., Girard A., Sachidanandam R., Hannon G.J., Aravin A.A.
    Genes Dev. 23:1749-1762(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN METHYLATION OF PIWI PROTEINS.

Entry informationi

Entry nameiMEP50_MOUSE
AccessioniPrimary (citable) accession number: Q99J09
Secondary accession number(s): Q3TFJ1, Q8BSH8, Q9CZY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.