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Protein

Methylosome protein 50

Gene

Wdr77

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed:19584108).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • negative regulation of cell proliferation Source: MGI
  • negative regulation of epithelial cell proliferation involved in prostate gland development Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI
  • secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-3214858 RMTs methylate histone arginines

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome protein 50
Short name:
MEP-50
Alternative name(s):
WD repeat-containing protein 77
Gene namesi
Name:Wdr77
Synonyms:Mep50
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1917715 Wdr77

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510751 – 342Methylosome protein 50Add BLAST342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99J09
MaxQBiQ99J09
PaxDbiQ99J09
PeptideAtlasiQ99J09
PRIDEiQ99J09

PTM databases

iPTMnetiQ99J09
PhosphoSitePlusiQ99J09

Expressioni

Gene expression databases

BgeeiENSMUSG00000000561
CleanExiMM_WDR77
ExpressionAtlasiQ99J09 baseline and differential
GenevisibleiQ99J09 MM

Interactioni

Subunit structurei

Component of the methylosome complex composed of PRMT5, WDR77 and CLNS1A (By similarity). Found in a complex composed of PRMT5, WDR77 and RIOK1 (By similarity). RIOK1 and CLNS1A bound directly to PRMT5 at the same binding site, in a mutually exclusive manner, which allows the recruitment of distinct methylation substrates, such as nucleolin/NCL and Sm proteins, respectively (By similarity). Found in a complex with the component of the methylosome, PRMT5, CLNS1A, WDR77, PRMT1 and ERH. Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4 (PubMed:16712789). Interacts with CTDP1 and LSM11. Interacts with APEX1, AR and NKX3-1. Interacts with CHTOP.By similarity1 Publication

Protein-protein interaction databases

BioGridi214072, 1 interactor
IntActiQ99J09, 4 interactors
MINTiQ99J09
STRINGi10090.ENSMUSP00000010278

Structurei

3D structure databases

ProteinModelPortaliQ99J09
SMRiQ99J09
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati22 – 75WD 1Add BLAST54
Repeati78 – 116WD 2Add BLAST39
Repeati123 – 162WD 3Add BLAST40
Repeati165 – 205WD 4Add BLAST41
Repeati209 – 250WD 5Add BLAST42
Repeati253 – 293WD 6Add BLAST41
Repeati295 – 330WD 7Add BLAST36

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410KCXQ Eukaryota
ENOG4111F0R LUCA
GeneTreeiENSGT00390000010711
HOGENOMiHOG000035094
HOVERGENiHBG052458
InParanoidiQ99J09
KOiK13221
OMAiDTKSASC
OrthoDBiEOG091G0T6F
PhylomeDBiQ99J09
TreeFamiTF325967

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 2 hits
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q99J09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKDTPPPLV PPAAREWNLP PNAPACMERQ LEAARYRSDG SLLLGVSSLS
60 70 80 90 100
GRCWVGSLWF FKDPSAAPNE GFCSAGVQTE AGVADLTWVG DKGILVASDS
110 120 130 140 150
GAVELWELDE NETLIVSKFC KYEHDDIVST VTVLSSGTQA VSGSKDCCIK
160 170 180 190 200
IWDLAQQVSL NSYRAHAGQV TCVAASPHKD SVFLSCSEDS RILLWDTRCP
210 220 230 240 250
KPASQMACNA SGYLPTALAW HPQQSEVFVF GDENGSVSLV DTKNASCTLS
260 270 280 290 300
SAVHSQGVTR LVFSPHSVPL LTSLSEDCSL AVLDSSLSEV FRSRAHRDFV
310 320 330 340
RDATWSPLNH SLLTTVGWDH QVIHHVVPLE PLPNPGPDSV VE
Length:342
Mass (Da):36,943
Last modified:June 1, 2001 - v1
Checksum:iE9C52BC4D6E5AC36
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti267 – 285SVPLL…AVLDS → RCCVSPGTWKGWVGTVVKE in AAH05755 (PubMed:15489334).CuratedAdd BLAST19
Sequence conflicti286 – 342Missing in AAH05755 (PubMed:15489334).CuratedAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012014 mRNA Translation: BAB27975.1
AK032897 mRNA Translation: BAC28076.1
AK048488 mRNA Translation: BAC33350.1
AK050391 mRNA Translation: BAC34232.1
AK163839 mRNA Translation: BAE37512.1
AK169128 mRNA Translation: BAE40907.1
BC005755 mRNA Translation: AAH05755.1
CCDSiCCDS17715.1
RefSeqiNP_081708.1, NM_027432.3
UniGeneiMm.5110

Genome annotation databases

EnsembliENSMUST00000010278; ENSMUSP00000010278; ENSMUSG00000000561
GeneIDi70465
KEGGimmu:70465
UCSCiuc008qvo.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMEP50_MOUSE
AccessioniPrimary (citable) accession number: Q99J09
Secondary accession number(s): Q3TFJ1, Q8BSH8, Q9CZY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health