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Q99FX7 (NSP1_ROTSF) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 1

Short name=NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name=NS53
OrganismRotavirus A (strain SA11-FEM G3-P6[1]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Simian Agent 11 (strain FEM))
Taxonomic identifier10925 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.

Subunit structure

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.

Subcellular location

Host cytoplasmhost cytoskeleton By similarity.

Domain

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similarities

Belongs to the rotavirus A NSP1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Non-structural protein 1
PRO_0000369094

Regions

Zinc finger42 – 7938 Potential
Region1 – 8181RNA-binding By similarity
Region82 – 17796Important for cytoskeleton localization By similarity
Region317 – 496180Interaction with IRF3 By similarity

Natural variations

Natural variant410 – 4145MLILG → LRGGV in strain: Isolate 30-1A.
Natural variant415 – 49682Missing in strain: Isolate 30-1A.

Sequences

Sequence LengthMass (Da)Tools
Q99FX7 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 5A9AC3B24A15B1FA

FASTA49658,623
        10         20         30         40         50         60 
MATFKDACFH YRRLTALNRR LCNIGANSIC MPVPDEKIKG WCLECCQIAD LTHCYGCSLP 

        70         80         90        100        110        120 
HVCKWCVQNR RCFLDNEPHL LKLRTVKHPI TKDKLQCIID LYNIIFPIND KVIRKFERMI 

       130        140        150        160        170        180 
KQRKCRNQYK IEWYNHLLLP ITLNAAAFKF DENNLYYVFG LYEKSVSDIY APYRIVNFIN 

       190        200        210        220        230        240 
EFDKLLLDDI NFTRMSNLPI ELRNHYAKKY FQLSRLPSSK LKQIYFSDFT KETVIFNTYT 

       250        260        270        280        290        300 
KTPGRSIYRN VTEFNWRDEL ELYSDLKNDK NKLIAAMMTS KYTRFYAHDN NFGRLKMTIF 

       310        320        330        340        350        360 
ELGHHCQPNY VASNHPGNAS DIQYCKWCNI KYFLSKIDWR IRDMYNLLME FIKDCYKSNV 

       370        380        390        400        410        420 
NVGHCSSVEN IYPLIKRLIW SLFTNHMDQT IEEVFNHMSP VSVEGTNVIM LILGLNISLY 

       430        440        450        460        470        480 
NEIKRTLNVD SIPMVLNLNE FSSIVKSISS KWYNVDELDK LPMSIKSTEE LIEMKNSGTL 

       490 
TEEFELLISN SEDDNE 

« Hide

References

[1]"Effect of intragenic rearrangement and changes in the 3' consensus sequence on NSP1 expression and rotavirus replication."
Patton J.T., Taraporewala Z.F., Chen D., Chizhikov V., Jones M.T., Elhelu A., Collins M., Kearney K., Wagner M., Hoshino Y., Gouvea V.
J. Virol. 75:2076-2086(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate 30-19 and Isolate 30-1A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF290881 Genomic RNA. Translation: AAK14069.1.
AF290882 Genomic RNA. Translation: AAK14070.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNSP1_ROTSF
AccessionPrimary (citable) accession number: Q99FX7
Secondary accession number(s): Q99FX6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 1, 2001
Last modified: February 19, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families