SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q99FX5

- NSP1_ROTS4

UniProt

Q99FX5 - NSP1_ROTS4

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (strain SA11-4F G3-P6[1]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Simian Agent 11 (strain 4F))
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri42 – 7938 Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein binding Source: UniProtKB
  3. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
  3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1
Short name:
NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name:
NS53
OrganismiRotavirus A (strain SA11-4F G3-P6[1]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Simian Agent 11 (strain 4F))
Taxonomic identifieri36436 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Subcellular locationi

Host cytoplasmhost cytoskeleton 1 Publication

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cytoskeleton Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Non-structural protein 1PRO_0000367820Add
BLAST

Interactioni

Subunit structurei

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation.2 Publications

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8181RNA-bindingAdd
BLAST
Regioni82 – 17796Important for cytoskeleton localizationAdd
BLAST
Regioni317 – 496180Interaction with IRF3Add
BLAST

Domaini

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.

Sequence similaritiesi

Belongs to the rotavirus A NSP1 family.

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99FX5-1 [UniParc]FASTAAdd to Basket

« Hide

MATFKDACFH YRRLTALNRR LCNIGANSIC MPVPDAKIKG WCLECCQIAD    50
LTHCYGCSLP HVCKWCVQNR RCFLDNEPHL LKLRTVKHPI TKDKLQCIID 100
LYNIIFPIND KVIRKFERMI KQRKCRNQYK IEWYNHLLLP ITLNAAAFKF 150
DENNLYYVFG LYEKSVSDIY APYRIVNFIN EFDKLLLDDI NFTRMSNLPI 200
ELRNHYAKKY FQLSRLPSSK LKQIYFSDFT KETVIFNTYT KTPGRSIYRN 250
VTEFNWRDEL ELYSDLKNDK NKLIAAMMTS KYTRFYAHDN NFGRLKMTIF 300
ELGHHCQPNY VASNHPGNAS DIQYCKWCNI KYFLSKIDWR IRDMYNLLME 350
FIKDCYKSNV NVGHCSSVEN IYPLIKRLIW SLFTNHMDQT IEEVFNHMSP 400
VSVEGTNVIM LILGLNISLY NEIKRTLNVD SIPMVLNLNE FSSIVKSISS 450
KWYNVDELDK LPMSIKSTEE LIEMKNSGTL TEEFELLISN SEDDNE 496
Length:496
Mass (Da):58,565
Last modified:June 1, 2001 - v1
Checksum:i5877EA0A0C078CAD
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti479 – 4791T → I in strain: Isolate 5S.
Natural varianti480 – 49617Missing in strain: Isolate 5S.
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF290883 Genomic RNA. Translation: AAK14071.1.
AF290884 Genomic RNA. Translation: AAK14072.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF290883 Genomic RNA. Translation: AAK14071.1 .
AF290884 Genomic RNA. Translation: AAK14072.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002148. Rotavirus_NSP1.
[Graphical view ]
Pfami PF00981. Rota_NS53. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Effect of intragenic rearrangement and changes in the 3' consensus sequence on NSP1 expression and rotavirus replication."
    Patton J.T., Taraporewala Z.F., Chen D., Chizhikov V., Jones M.T., Elhelu A., Collins M., Kearney K., Wagner M., Hoshino Y., Gouvea V.
    J. Virol. 75:2076-2086(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Deletion mapping of the rotavirus metalloprotein NS53 (NSP1): the conserved cysteine-rich region is essential for virus-specific RNA binding."
    Hua J.J., Chen X., Patton J.T.
    J. Virol. 68:3990-4000(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING, SUBCELLULAR LOCATION.
  3. "Rotavirus nonstructural protein 1 subverts innate immune response by inducing degradation of IFN regulatory factor 3."
    Barro M., Patton J.T.
    Proc. Natl. Acad. Sci. U.S.A. 102:4114-4119(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HUMAN IRF3.
  4. "Zinc-binding domain of rotavirus NSP1 is required for proteasome-dependent degradation of IRF3 and autoregulatory NSP1 stability."
    Graff J.W., Ewen J., Ettayebi K., Hardy M.E.
    J. Gen. Virol. 88:613-620(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Rotavirus NSP1 inhibits expression of type I interferon by antagonizing the function of interferon regulatory factors IRF3, IRF5, and IRF7."
    Barro M., Patton J.T.
    J. Virol. 81:4473-4481(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HUMAN IRF7.

Entry informationi

Entry nameiNSP1_ROTS4
AccessioniPrimary (citable) accession number: Q99FX5
Secondary accession number(s): Q99FX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 1, 2001
Last modified: June 11, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi