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Q99FX5

- NSP1_ROTS4

UniProt

Q99FX5 - NSP1_ROTS4

Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (strain SA11-4F G3-P6[1]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Simian Agent 11 (strain 4F))
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri42 – 7938Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: UniProtKB
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. suppression by virus of host IRF3 activity Source: UniProtKB-KW
    2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
    3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 1
    Short name:
    NSP1
    Alternative name(s):
    NCVP2
    Non-structural RNA-binding protein 53
    Short name:
    NS53
    OrganismiRotavirus A (strain SA11-4F G3-P6[1]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Simian Agent 11 (strain 4F))
    Taxonomic identifieri36436 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

    Subcellular locationi

    Host cytoplasmhost cytoskeleton 1 Publication

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-KW
    2. host cytoskeleton Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 496496Non-structural protein 1PRO_0000367820Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation.2 Publications

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 8181RNA-bindingAdd
    BLAST
    Regioni82 – 17796Important for cytoskeleton localizationAdd
    BLAST
    Regioni317 – 496180Interaction with IRF3Add
    BLAST

    Domaini

    The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.By similarity

    Sequence similaritiesi

    Belongs to the rotavirus A NSP1 family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri42 – 7938Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR002148. Rotavirus_NSP1.
    [Graphical view]
    PfamiPF00981. Rota_NS53. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99FX5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATFKDACFH YRRLTALNRR LCNIGANSIC MPVPDAKIKG WCLECCQIAD    50
    LTHCYGCSLP HVCKWCVQNR RCFLDNEPHL LKLRTVKHPI TKDKLQCIID 100
    LYNIIFPIND KVIRKFERMI KQRKCRNQYK IEWYNHLLLP ITLNAAAFKF 150
    DENNLYYVFG LYEKSVSDIY APYRIVNFIN EFDKLLLDDI NFTRMSNLPI 200
    ELRNHYAKKY FQLSRLPSSK LKQIYFSDFT KETVIFNTYT KTPGRSIYRN 250
    VTEFNWRDEL ELYSDLKNDK NKLIAAMMTS KYTRFYAHDN NFGRLKMTIF 300
    ELGHHCQPNY VASNHPGNAS DIQYCKWCNI KYFLSKIDWR IRDMYNLLME 350
    FIKDCYKSNV NVGHCSSVEN IYPLIKRLIW SLFTNHMDQT IEEVFNHMSP 400
    VSVEGTNVIM LILGLNISLY NEIKRTLNVD SIPMVLNLNE FSSIVKSISS 450
    KWYNVDELDK LPMSIKSTEE LIEMKNSGTL TEEFELLISN SEDDNE 496
    Length:496
    Mass (Da):58,565
    Last modified:June 1, 2001 - v1
    Checksum:i5877EA0A0C078CAD
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti479 – 4791T → I in strain: Isolate 5S.
    Natural varianti480 – 49617Missing in strain: Isolate 5S.
    Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF290883 Genomic RNA. Translation: AAK14071.1.
    AF290884 Genomic RNA. Translation: AAK14072.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF290883 Genomic RNA. Translation: AAK14071.1 .
    AF290884 Genomic RNA. Translation: AAK14072.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002148. Rotavirus_NSP1.
    [Graphical view ]
    Pfami PF00981. Rota_NS53. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Effect of intragenic rearrangement and changes in the 3' consensus sequence on NSP1 expression and rotavirus replication."
      Patton J.T., Taraporewala Z.F., Chen D., Chizhikov V., Jones M.T., Elhelu A., Collins M., Kearney K., Wagner M., Hoshino Y., Gouvea V.
      J. Virol. 75:2076-2086(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Deletion mapping of the rotavirus metalloprotein NS53 (NSP1): the conserved cysteine-rich region is essential for virus-specific RNA binding."
      Hua J.J., Chen X., Patton J.T.
      J. Virol. 68:3990-4000(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA-BINDING, SUBCELLULAR LOCATION.
    3. "Rotavirus nonstructural protein 1 subverts innate immune response by inducing degradation of IFN regulatory factor 3."
      Barro M., Patton J.T.
      Proc. Natl. Acad. Sci. U.S.A. 102:4114-4119(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HUMAN IRF3.
    4. "Zinc-binding domain of rotavirus NSP1 is required for proteasome-dependent degradation of IRF3 and autoregulatory NSP1 stability."
      Graff J.W., Ewen J., Ettayebi K., Hardy M.E.
      J. Gen. Virol. 88:613-620(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Rotavirus NSP1 inhibits expression of type I interferon by antagonizing the function of interferon regulatory factors IRF3, IRF5, and IRF7."
      Barro M., Patton J.T.
      J. Virol. 81:4473-4481(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HUMAN IRF7.

    Entry informationi

    Entry nameiNSP1_ROTS4
    AccessioniPrimary (citable) accession number: Q99FX5
    Secondary accession number(s): Q99FX4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3