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Q99CW3

- GCNT3_BHV4D

UniProt

Q99CW3 - GCNT3_BHV4D

Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Gene

Bo17

Organism
Bovine herpesvirus 4 (strain DN-599) (BoHV-4) (Movar virus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Non-essential glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans. Contributes to the post-translational modifications of structural proteins.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

    Pathwayi

    GO - Molecular functioni

    1. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
    2. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT14. Glycosyltransferase Family 14.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC:2.4.1.102, EC:2.4.1.150)
    Alternative name(s):
    C2GnT-mucin type
    Short name:
    C2GnT-M
    Gene namesi
    Name:Bo17
    OrganismiBovine herpesvirus 4 (strain DN-599) (BoHV-4) (Movar virus)
    Taxonomic identifieri10355 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
    Panthera leo (Lion) [TaxID: 9689]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host Golgi apparatus, Host membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 440440Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferasePRO_0000288552Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi72 – 721N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi73 ↔ 230By similarity
    Glycosylationi108 – 1081N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi164 ↔ 384By similarity
    Disulfide bondi185 ↔ 212By similarity
    Disulfide bondi393 ↔ 425By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Developmental stagei

    Expressed during BHV-4 repkication (at protein level).

    Structurei

    3D structure databases

    ProteinModelPortaliQ99CW3.
    SMRiQ99CW3. Positions 70-435.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini31 – 440410LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3018Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 14 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR003406. Glyco_trans_14.
    [Graphical view]
    PfamiPF02485. Branch. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99CW3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMAGWKKKL CRGHHLWALG CYMLLAVVSL RLSLRFKCDV DSLDLESRDF    50
    QSQHCRDMLY NSLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRSP 100
    LTGTYYLNIT RDCERFKAQR KFIQFPLSKE ELDFPIAYSM VVHEKIENFE 150
    RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK AIISCFPNVF MASKLVPVVY 200
    ASWSRVQADL NCMEDLLQSS VPWKYLLNTC GTDFPIKTNA EMVLALKMLK 250
    GKNSMESEVP SESKKNRWKY RYEVTDTLYP TSKMKDPPPD NLPMFTGNAY 300
    FVASRAFVQH VLDNPKSQRL VEWVKDTYSP DEHLWATLQR APWMPGSVPS 350
    HPKYHISDMT AIARLVKWQY HEGDVSMGAP YAPCSGIHRR AICIYGAGDL 400
    YWILQNHHLL ANKFDPRVDD NVLQCLEEYL RHKAIYGTEL 440
    Length:440
    Mass (Da):50,764
    Last modified:June 1, 2001 - v1
    Checksum:iC88B3EAB8FDB8DD9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF318573 Genomic DNA. Translation: AAK07999.1.
    AF465332 Genomic DNA. Translation: AAO22159.1.
    RefSeqiNP_076572.1. NC_002665.1.

    Genome annotation databases

    GeneIDi1684881.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF318573 Genomic DNA. Translation: AAK07999.1 .
    AF465332 Genomic DNA. Translation: AAO22159.1 .
    RefSeqi NP_076572.1. NC_002665.1.

    3D structure databases

    ProteinModelPortali Q99CW3.
    SMRi Q99CW3. Positions 70-435.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT14. Glycosyltransferase Family 14.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1684881.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Family and domain databases

    InterProi IPR003406. Glyco_trans_14.
    [Graphical view ]
    Pfami PF02485. Branch. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of bovine herpesvirus 4, a bovine Rhadinovirus, and identification of an origin of DNA replication."
      Zimmermann W., Broll H., Ehlers B., Buhk H.-J., Rosenthal A., Goltz M.
      J. Virol. 75:1186-1194(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo."
      Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A.
      J. Virol. 77:1784-1792(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiGCNT3_BHV4D
    AccessioniPrimary (citable) accession number: Q99CW3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Was acquired from an ancestor of the African buffalo around 1.5 million years ago.

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3