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Q99CW3 (GCNT3_BHV4D) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

EC=2.4.1.102
EC=2.4.1.150
Alternative name(s):
C2GnT-mucin type
Short name=C2GnT-M
Gene names
Name:Bo17
OrganismBovine herpesvirus 4 (strain DN-599) (BoHV-4) (Movar virus)
Taxonomic identifier10355 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostBos taurus (Bovine) [TaxID: 9913]
Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
Panthera leo (Lion) [TaxID: 9689]

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-essential glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans. Contributes to the post-translational modifications of structural proteins.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Host Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Developmental stage

Expressed during BHV-4 repkication (at protein level).

Miscellaneous

Was acquired from an ancestor of the African buffalo around 1.5 million years ago.

Sequence similarities

Belongs to the glycosyltransferase 14 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
PRO_0000288552

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3018Helical; Signal-anchor for type II membrane protein; Potential
Topological domain31 – 440410Lumenal Potential

Amino acid modifications

Glycosylation721N-linked (GlcNAc...); by host Potential
Glycosylation1081N-linked (GlcNAc...); by host Potential
Disulfide bond73 ↔ 230 By similarity
Disulfide bond164 ↔ 384 By similarity
Disulfide bond185 ↔ 212 By similarity
Disulfide bond393 ↔ 425 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99CW3 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: C88B3EAB8FDB8DD9

FASTA44050,764
        10         20         30         40         50         60 
MKMAGWKKKL CRGHHLWALG CYMLLAVVSL RLSLRFKCDV DSLDLESRDF QSQHCRDMLY 

        70         80         90        100        110        120 
NSLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRSP LTGTYYLNIT RDCERFKAQR 

       130        140        150        160        170        180 
KFIQFPLSKE ELDFPIAYSM VVHEKIENFE RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK 

       190        200        210        220        230        240 
AIISCFPNVF MASKLVPVVY ASWSRVQADL NCMEDLLQSS VPWKYLLNTC GTDFPIKTNA 

       250        260        270        280        290        300 
EMVLALKMLK GKNSMESEVP SESKKNRWKY RYEVTDTLYP TSKMKDPPPD NLPMFTGNAY 

       310        320        330        340        350        360 
FVASRAFVQH VLDNPKSQRL VEWVKDTYSP DEHLWATLQR APWMPGSVPS HPKYHISDMT 

       370        380        390        400        410        420 
AIARLVKWQY HEGDVSMGAP YAPCSGIHRR AICIYGAGDL YWILQNHHLL ANKFDPRVDD 

       430        440 
NVLQCLEEYL RHKAIYGTEL 

« Hide

References

[1]"Genome sequence of bovine herpesvirus 4, a bovine Rhadinovirus, and identification of an origin of DNA replication."
Zimmermann W., Broll H., Ehlers B., Buhk H.-J., Rosenthal A., Goltz M.
J. Virol. 75:1186-1194(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo."
Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A.
J. Virol. 77:1784-1792(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF318573 Genomic DNA. Translation: AAK07999.1.
AF465332 Genomic DNA. Translation: AAO22159.1.
RefSeqNP_076572.1. NC_002665.1.

3D structure databases

ProteinModelPortalQ99CW3.
SMRQ99CW3. Positions 70-435.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT14. Glycosyltransferase Family 14.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1684881.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR003406. Glyco_trans_14.
[Graphical view]
PfamPF02485. Branch. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGCNT3_BHV4D
AccessionPrimary (citable) accession number: Q99CW3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 1, 2001
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways