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Q99999

- G3ST1_HUMAN

UniProt

Q99999 - G3ST1_HUMAN

Protein

Galactosylceramide sulfotransferase

Gene

GAL3ST1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes By similarity. Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro).By similarity1 Publication

    Catalytic activityi

    3'-phosphoadenylyl sulfate + a galactosylceramide = adenosine 3',5'-bisphosphate + a galactosylceramidesulfate.
    3'-phosphoadenylyl sulfate + monogalactosylalkylacylglycerol = adenosine 3',5'-bisphosphate + monogalactosylalkylacylglycerol sulfate.

    Pathwayi

    GO - Molecular functioni

    1. galactosylceramide sulfotransferase activity Source: UniProtKB-EC
    2. sulfotransferase activity Source: ProtInc

    GO - Biological processi

    1. galactosylceramide biosynthetic process Source: Ensembl
    2. glycosphingolipid metabolic process Source: Reactome
    3. myelination Source: Ensembl
    4. protein N-linked glycosylation Source: ProtInc
    5. small molecule metabolic process Source: Reactome
    6. spermatogenesis Source: Ensembl
    7. sphingolipid metabolic process Source: Reactome

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Enzyme and pathway databases

    BRENDAi2.8.2.11. 2681.
    ReactomeiREACT_116105. Glycosphingolipid metabolism.
    UniPathwayiUPA00222.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galactosylceramide sulfotransferase (EC:2.8.2.11)
    Short name:
    GalCer sulfotransferase
    Alternative name(s):
    3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase
    3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
    Cerebroside sulfotransferase
    Gene namesi
    Name:GAL3ST1
    Synonyms:CST
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 22

    Organism-specific databases

    HGNCiHGNC:24240. GAL3ST1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: Reactome
    2. integral component of plasma membrane Source: ProtInc
    3. membrane Source: ProtInc

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134889661.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 423423Galactosylceramide sulfotransferasePRO_0000085201Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi66 – 661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ99999.
    PRIDEiQ99999.

    PTM databases

    PhosphoSiteiQ99999.

    Expressioni

    Tissue specificityi

    Expressed in kidney proximal tubule, gastric mucosa and adenocarcinoma. Highly expressed in renal cell carcinoma cell lines.

    Gene expression databases

    ArrayExpressiQ99999.
    BgeeiQ99999.
    CleanExiHS_GAL3ST1.
    GenevestigatoriQ99999.

    Organism-specific databases

    HPAiHPA001220.

    Interactioni

    Protein-protein interaction databases

    BioGridi114891. 1 interaction.
    STRINGi9606.ENSP00000343234.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99999.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini36 – 423388LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG45565.
    HOGENOMiHOG000232148.
    HOVERGENiHBG051681.
    InParanoidiQ99999.
    KOiK01019.
    OMAiIQDSAMQ.
    OrthoDBiEOG7K0ZF1.
    PhylomeDBiQ99999.
    TreeFamiTF314802.

    Family and domain databases

    InterProiIPR009729. Gal-3-0_sulfotransfrase.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR14647. PTHR14647. 1 hit.
    PfamiPF06990. Gal-3-0_sulfotr. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q99999-1 [UniParc]FASTAAdd to Basket

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    MLPPQKKPWE SMAKGLVLGA LFTSFLLLVY SYAVPPLHAG LASTTPEAAA    50
    SCSPPALEPE AVIRANGSAG ECQPRRNIVF LKTHKTASST LLNILFRFGQ 100
    KHRLKFAFPN GRNDFDYPTF FARSLVQDYR PGACFNIICN HMRFHYDEVR 150
    GLVPTNAIFI TVLRDPARLF ESSFHYFGPV VPLTWKLSAG DKLTEFLQDP 200
    DRYYDPNGFN AHYLRNLLFF DLGYDNSLDP SSPQVQEHIL EVERRFHLVL 250
    LQEYFDESLV LLKDLLCWEL EDVLYFKLNA RRDSPVPRLS GELYGRATAW 300
    NMLDSHLYRH FNASFWRKVE AFGRERMARE VAALRHANER MRTICIDGGH 350
    AVDAAAIQDE AMQPWQPLGT KSILGYNLKK SIGQRHAQLC RRMLTPEIQY 400
    LMDLGANLWV TKLWKFIRDF LRW 423
    Length:423
    Mass (Da):48,764
    Last modified:May 1, 1997 - v1
    Checksum:i0FD4F2E374447C56
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti281 – 2822RR → LN AA sequence (PubMed:9030544)Curated
    Sequence conflicti418 – 4181R → L AA sequence (PubMed:9030544)Curated
    Sequence conflicti422 – 4221R → E AA sequence (PubMed:9030544)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti29 – 291V → M.2 Publications
    Corresponds to variant rs2267161 [ dbSNP | Ensembl ].
    VAR_013684

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D88667 mRNA. Translation: BAA13673.1.
    AB029901 Genomic DNA. Translation: BAA89503.1.
    CR456350 mRNA. Translation: CAG30236.1.
    BC014649 mRNA. Translation: AAH14649.1.
    BC019077 mRNA. Translation: AAH19077.1.
    CCDSiCCDS13879.1.
    RefSeqiNP_004852.1. NM_004861.1.
    XP_005261905.1. XM_005261848.1.
    XP_005261910.1. XM_005261853.1.
    XP_005261913.1. XM_005261856.1.
    UniGeneiHs.17958.

    Genome annotation databases

    EnsembliENST00000338911; ENSP00000343234; ENSG00000128242.
    ENST00000401975; ENSP00000384388; ENSG00000128242.
    ENST00000402321; ENSP00000385735; ENSG00000128242.
    ENST00000402369; ENSP00000384122; ENSG00000128242.
    ENST00000406361; ENSP00000385207; ENSG00000128242.
    ENST00000406955; ENSP00000385825; ENSG00000128242.
    GeneIDi9514.
    KEGGihsa:9514.
    UCSCiuc003aig.1. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D88667 mRNA. Translation: BAA13673.1 .
    AB029901 Genomic DNA. Translation: BAA89503.1 .
    CR456350 mRNA. Translation: CAG30236.1 .
    BC014649 mRNA. Translation: AAH14649.1 .
    BC019077 mRNA. Translation: AAH19077.1 .
    CCDSi CCDS13879.1.
    RefSeqi NP_004852.1. NM_004861.1.
    XP_005261905.1. XM_005261848.1.
    XP_005261910.1. XM_005261853.1.
    XP_005261913.1. XM_005261856.1.
    UniGenei Hs.17958.

    3D structure databases

    ProteinModelPortali Q99999.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114891. 1 interaction.
    STRINGi 9606.ENSP00000343234.

    PTM databases

    PhosphoSitei Q99999.

    Proteomic databases

    PaxDbi Q99999.
    PRIDEi Q99999.

    Protocols and materials databases

    DNASUi 9514.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000338911 ; ENSP00000343234 ; ENSG00000128242 .
    ENST00000401975 ; ENSP00000384388 ; ENSG00000128242 .
    ENST00000402321 ; ENSP00000385735 ; ENSG00000128242 .
    ENST00000402369 ; ENSP00000384122 ; ENSG00000128242 .
    ENST00000406361 ; ENSP00000385207 ; ENSG00000128242 .
    ENST00000406955 ; ENSP00000385825 ; ENSG00000128242 .
    GeneIDi 9514.
    KEGGi hsa:9514.
    UCSCi uc003aig.1. human.

    Organism-specific databases

    CTDi 9514.
    GeneCardsi GC22M030950.
    HGNCi HGNC:24240. GAL3ST1.
    HPAi HPA001220.
    MIMi 602300. gene.
    neXtProti NX_Q99999.
    PharmGKBi PA134889661.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG45565.
    HOGENOMi HOG000232148.
    HOVERGENi HBG051681.
    InParanoidi Q99999.
    KOi K01019.
    OMAi IQDSAMQ.
    OrthoDBi EOG7K0ZF1.
    PhylomeDBi Q99999.
    TreeFami TF314802.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .
    BRENDAi 2.8.2.11. 2681.
    Reactomei REACT_116105. Glycosphingolipid metabolism.

    Miscellaneous databases

    GeneWikii GAL3ST1.
    GenomeRNAii 9514.
    NextBioi 35650.
    PROi Q99999.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99999.
    Bgeei Q99999.
    CleanExi HS_GAL3ST1.
    Genevestigatori Q99999.

    Family and domain databases

    InterProi IPR009729. Gal-3-0_sulfotransfrase.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR14647. PTHR14647. 1 hit.
    Pfami PF06990. Gal-3-0_sulfotr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of cDNA encoding human 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase."
      Honke K., Tsuda M., Hirahara Y., Ishii A., Makita A., Wada Y.
      J. Biol. Chem. 272:4864-4868(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-14; 86-101; 102-105; 187-192; 278-286; 372-379 AND 416-423.
      Tissue: Renal cell carcinoma.
    2. "Cancer-associated alternative usage of multiple promoters of human GalCer sulfotransferase gene."
      Tsuda M., Egashira M., Niikawa N., Wada Y., Honke K.
      Eur. J. Biochem. 267:2672-2679(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Kidney proximal tubule.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29.
      Tissue: Kidney.
    5. "Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal cancer cells."
      Honke K., Yamane M., Ishii A., Kobayashi T., Makita A.
      J. Biochem. 119:421-427(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiG3ST1_HUMAN
    AccessioniPrimary (citable) accession number: Q99999
    Secondary accession number(s): Q96C63
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 26, 2002
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 22
      Human chromosome 22: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3