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Protein

Galactosylceramide sulfotransferase

Gene

GAL3ST1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes (By similarity). Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro).By similarity1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + a galactosylceramide = adenosine 3',5'-bisphosphate + a galactosylceramidesulfate.
3'-phosphoadenylyl sulfate + monogalactosylalkylacylglycerol = adenosine 3',5'-bisphosphate + monogalactosylalkylacylglycerol sulfate.

Pathwayi

GO - Molecular functioni

  1. galactosylceramide sulfotransferase activity Source: UniProtKB-EC
  2. sulfotransferase activity Source: ProtInc

GO - Biological processi

  1. galactosylceramide biosynthetic process Source: Ensembl
  2. myelination Source: Ensembl
  3. protein N-linked glycosylation Source: ProtInc
  4. spermatogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BRENDAi2.8.2.11. 2681.
UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylceramide sulfotransferase (EC:2.8.2.11)
Short name:
GalCer sulfotransferase
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase
3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
Cerebroside sulfotransferase
Gene namesi
Name:GAL3ST1
Synonyms:CST
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 22

Organism-specific databases

HGNCiHGNC:24240. GAL3ST1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei15 – 3521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini36 – 423388LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of plasma membrane Source: ProtInc
  3. membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134889661.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Galactosylceramide sulfotransferasePRO_0000085201Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi66 – 661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ99999.
PaxDbiQ99999.
PRIDEiQ99999.

PTM databases

PhosphoSiteiQ99999.

Expressioni

Tissue specificityi

Expressed in kidney proximal tubule, gastric mucosa and adenocarcinoma. Highly expressed in renal cell carcinoma cell lines.

Gene expression databases

BgeeiQ99999.
CleanExiHS_GAL3ST1.
ExpressionAtlasiQ99999. baseline and differential.
GenevestigatoriQ99999.

Organism-specific databases

HPAiHPA001220.

Interactioni

Protein-protein interaction databases

BioGridi114891. 7 interactions.
STRINGi9606.ENSP00000343234.

Structurei

3D structure databases

ProteinModelPortaliQ99999.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG45565.
GeneTreeiENSGT00390000013505.
HOGENOMiHOG000232148.
HOVERGENiHBG051681.
InParanoidiQ99999.
KOiK01019.
OMAiIQDSAMQ.
OrthoDBiEOG7K0ZF1.
PhylomeDBiQ99999.
TreeFamiTF314802.

Family and domain databases

InterProiIPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR14647. PTHR14647. 1 hit.
PfamiPF06990. Gal-3-0_sulfotr. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q99999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPPQKKPWE SMAKGLVLGA LFTSFLLLVY SYAVPPLHAG LASTTPEAAA
60 70 80 90 100
SCSPPALEPE AVIRANGSAG ECQPRRNIVF LKTHKTASST LLNILFRFGQ
110 120 130 140 150
KHRLKFAFPN GRNDFDYPTF FARSLVQDYR PGACFNIICN HMRFHYDEVR
160 170 180 190 200
GLVPTNAIFI TVLRDPARLF ESSFHYFGPV VPLTWKLSAG DKLTEFLQDP
210 220 230 240 250
DRYYDPNGFN AHYLRNLLFF DLGYDNSLDP SSPQVQEHIL EVERRFHLVL
260 270 280 290 300
LQEYFDESLV LLKDLLCWEL EDVLYFKLNA RRDSPVPRLS GELYGRATAW
310 320 330 340 350
NMLDSHLYRH FNASFWRKVE AFGRERMARE VAALRHANER MRTICIDGGH
360 370 380 390 400
AVDAAAIQDE AMQPWQPLGT KSILGYNLKK SIGQRHAQLC RRMLTPEIQY
410 420
LMDLGANLWV TKLWKFIRDF LRW
Length:423
Mass (Da):48,764
Last modified:May 1, 1997 - v1
Checksum:i0FD4F2E374447C56
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti281 – 2822RR → LN AA sequence (PubMed:9030544).Curated
Sequence conflicti418 – 4181R → L AA sequence (PubMed:9030544).Curated
Sequence conflicti422 – 4221R → E AA sequence (PubMed:9030544).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291V → M.2 Publications
Corresponds to variant rs2267161 [ dbSNP | Ensembl ].
VAR_013684

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88667 mRNA. Translation: BAA13673.1.
AB029901 Genomic DNA. Translation: BAA89503.1.
CR456350 mRNA. Translation: CAG30236.1.
BC014649 mRNA. Translation: AAH14649.1.
BC019077 mRNA. Translation: AAH19077.1.
CCDSiCCDS13879.1.
RefSeqiNP_004852.1. NM_004861.1.
XP_005261905.1. XM_005261848.1.
XP_005261910.1. XM_005261853.1.
XP_005261913.1. XM_005261856.1.
UniGeneiHs.17958.

Genome annotation databases

EnsembliENST00000338911; ENSP00000343234; ENSG00000128242.
ENST00000401975; ENSP00000384388; ENSG00000128242.
ENST00000402321; ENSP00000385735; ENSG00000128242.
ENST00000402369; ENSP00000384122; ENSG00000128242.
ENST00000406361; ENSP00000385207; ENSG00000128242.
ENST00000406955; ENSP00000385825; ENSG00000128242.
GeneIDi9514.
KEGGihsa:9514.
UCSCiuc003aig.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88667 mRNA. Translation: BAA13673.1.
AB029901 Genomic DNA. Translation: BAA89503.1.
CR456350 mRNA. Translation: CAG30236.1.
BC014649 mRNA. Translation: AAH14649.1.
BC019077 mRNA. Translation: AAH19077.1.
CCDSiCCDS13879.1.
RefSeqiNP_004852.1. NM_004861.1.
XP_005261905.1. XM_005261848.1.
XP_005261910.1. XM_005261853.1.
XP_005261913.1. XM_005261856.1.
UniGeneiHs.17958.

3D structure databases

ProteinModelPortaliQ99999.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114891. 7 interactions.
STRINGi9606.ENSP00000343234.

PTM databases

PhosphoSiteiQ99999.

Proteomic databases

MaxQBiQ99999.
PaxDbiQ99999.
PRIDEiQ99999.

Protocols and materials databases

DNASUi9514.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338911; ENSP00000343234; ENSG00000128242.
ENST00000401975; ENSP00000384388; ENSG00000128242.
ENST00000402321; ENSP00000385735; ENSG00000128242.
ENST00000402369; ENSP00000384122; ENSG00000128242.
ENST00000406361; ENSP00000385207; ENSG00000128242.
ENST00000406955; ENSP00000385825; ENSG00000128242.
GeneIDi9514.
KEGGihsa:9514.
UCSCiuc003aig.1. human.

Organism-specific databases

CTDi9514.
GeneCardsiGC22M030950.
HGNCiHGNC:24240. GAL3ST1.
HPAiHPA001220.
MIMi602300. gene.
neXtProtiNX_Q99999.
PharmGKBiPA134889661.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG45565.
GeneTreeiENSGT00390000013505.
HOGENOMiHOG000232148.
HOVERGENiHBG051681.
InParanoidiQ99999.
KOiK01019.
OMAiIQDSAMQ.
OrthoDBiEOG7K0ZF1.
PhylomeDBiQ99999.
TreeFamiTF314802.

Enzyme and pathway databases

UniPathwayiUPA00222.
BRENDAi2.8.2.11. 2681.

Miscellaneous databases

GeneWikiiGAL3ST1.
GenomeRNAii9514.
NextBioi35650.
PROiQ99999.
SOURCEiSearch...

Gene expression databases

BgeeiQ99999.
CleanExiHS_GAL3ST1.
ExpressionAtlasiQ99999. baseline and differential.
GenevestigatoriQ99999.

Family and domain databases

InterProiIPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR14647. PTHR14647. 1 hit.
PfamiPF06990. Gal-3-0_sulfotr. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of cDNA encoding human 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase."
    Honke K., Tsuda M., Hirahara Y., Ishii A., Makita A., Wada Y.
    J. Biol. Chem. 272:4864-4868(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-14; 86-101; 102-105; 187-192; 278-286; 372-379 AND 416-423.
    Tissue: Renal cell carcinoma.
  2. "Cancer-associated alternative usage of multiple promoters of human GalCer sulfotransferase gene."
    Tsuda M., Egashira M., Niikawa N., Wada Y., Honke K.
    Eur. J. Biochem. 267:2672-2679(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Kidney proximal tubule.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29.
    Tissue: Kidney.
  5. "Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal cancer cells."
    Honke K., Yamane M., Ishii A., Kobayashi T., Makita A.
    J. Biochem. 119:421-427(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiG3ST1_HUMAN
AccessioniPrimary (citable) accession number: Q99999
Secondary accession number(s): Q96C63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: May 1, 1997
Last modified: March 4, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.