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Q99999

- G3ST1_HUMAN

UniProt

Q99999 - G3ST1_HUMAN

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Protein

Galactosylceramide sulfotransferase

Gene

GAL3ST1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes (By similarity). Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro).By similarity1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + a galactosylceramide = adenosine 3',5'-bisphosphate + a galactosylceramidesulfate.
3'-phosphoadenylyl sulfate + monogalactosylalkylacylglycerol = adenosine 3',5'-bisphosphate + monogalactosylalkylacylglycerol sulfate.

Pathwayi

GO - Molecular functioni

  1. galactosylceramide sulfotransferase activity Source: UniProtKB-EC
  2. sulfotransferase activity Source: ProtInc

GO - Biological processi

  1. galactosylceramide biosynthetic process Source: Ensembl
  2. glycosphingolipid metabolic process Source: Reactome
  3. myelination Source: Ensembl
  4. protein N-linked glycosylation Source: ProtInc
  5. small molecule metabolic process Source: Reactome
  6. spermatogenesis Source: Ensembl
  7. sphingolipid metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BRENDAi2.8.2.11. 2681.
ReactomeiREACT_116105. Glycosphingolipid metabolism.
UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylceramide sulfotransferase (EC:2.8.2.11)
Short name:
GalCer sulfotransferase
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase
3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
Cerebroside sulfotransferase
Gene namesi
Name:GAL3ST1
Synonyms:CST
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 22

Organism-specific databases

HGNCiHGNC:24240. GAL3ST1.

Subcellular locationi

GO - Cellular componenti

  1. Golgi membrane Source: Reactome
  2. integral component of plasma membrane Source: ProtInc
  3. membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134889661.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Galactosylceramide sulfotransferasePRO_0000085201Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi66 – 661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ99999.
PaxDbiQ99999.
PRIDEiQ99999.

PTM databases

PhosphoSiteiQ99999.

Expressioni

Tissue specificityi

Expressed in kidney proximal tubule, gastric mucosa and adenocarcinoma. Highly expressed in renal cell carcinoma cell lines.

Gene expression databases

BgeeiQ99999.
CleanExiHS_GAL3ST1.
ExpressionAtlasiQ99999. baseline and differential.
GenevestigatoriQ99999.

Organism-specific databases

HPAiHPA001220.

Interactioni

Protein-protein interaction databases

BioGridi114891. 7 interactions.
STRINGi9606.ENSP00000343234.

Structurei

3D structure databases

ProteinModelPortaliQ99999.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini36 – 423388LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei15 – 3521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG45565.
GeneTreeiENSGT00390000013505.
HOGENOMiHOG000232148.
HOVERGENiHBG051681.
InParanoidiQ99999.
KOiK01019.
OMAiIQDSAMQ.
OrthoDBiEOG7K0ZF1.
PhylomeDBiQ99999.
TreeFamiTF314802.

Family and domain databases

InterProiIPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR14647. PTHR14647. 1 hit.
PfamiPF06990. Gal-3-0_sulfotr. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q99999-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLPPQKKPWE SMAKGLVLGA LFTSFLLLVY SYAVPPLHAG LASTTPEAAA
60 70 80 90 100
SCSPPALEPE AVIRANGSAG ECQPRRNIVF LKTHKTASST LLNILFRFGQ
110 120 130 140 150
KHRLKFAFPN GRNDFDYPTF FARSLVQDYR PGACFNIICN HMRFHYDEVR
160 170 180 190 200
GLVPTNAIFI TVLRDPARLF ESSFHYFGPV VPLTWKLSAG DKLTEFLQDP
210 220 230 240 250
DRYYDPNGFN AHYLRNLLFF DLGYDNSLDP SSPQVQEHIL EVERRFHLVL
260 270 280 290 300
LQEYFDESLV LLKDLLCWEL EDVLYFKLNA RRDSPVPRLS GELYGRATAW
310 320 330 340 350
NMLDSHLYRH FNASFWRKVE AFGRERMARE VAALRHANER MRTICIDGGH
360 370 380 390 400
AVDAAAIQDE AMQPWQPLGT KSILGYNLKK SIGQRHAQLC RRMLTPEIQY
410 420
LMDLGANLWV TKLWKFIRDF LRW
Length:423
Mass (Da):48,764
Last modified:May 1, 1997 - v1
Checksum:i0FD4F2E374447C56
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti281 – 2822RR → LN AA sequence (PubMed:9030544)Curated
Sequence conflicti418 – 4181R → L AA sequence (PubMed:9030544)Curated
Sequence conflicti422 – 4221R → E AA sequence (PubMed:9030544)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291V → M.2 Publications
Corresponds to variant rs2267161 [ dbSNP | Ensembl ].
VAR_013684

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D88667 mRNA. Translation: BAA13673.1.
AB029901 Genomic DNA. Translation: BAA89503.1.
CR456350 mRNA. Translation: CAG30236.1.
BC014649 mRNA. Translation: AAH14649.1.
BC019077 mRNA. Translation: AAH19077.1.
CCDSiCCDS13879.1.
RefSeqiNP_004852.1. NM_004861.1.
XP_005261905.1. XM_005261848.1.
XP_005261910.1. XM_005261853.1.
XP_005261913.1. XM_005261856.1.
UniGeneiHs.17958.

Genome annotation databases

EnsembliENST00000338911; ENSP00000343234; ENSG00000128242.
ENST00000401975; ENSP00000384388; ENSG00000128242.
ENST00000402321; ENSP00000385735; ENSG00000128242.
ENST00000402369; ENSP00000384122; ENSG00000128242.
ENST00000406361; ENSP00000385207; ENSG00000128242.
ENST00000406955; ENSP00000385825; ENSG00000128242.
GeneIDi9514.
KEGGihsa:9514.
UCSCiuc003aig.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D88667 mRNA. Translation: BAA13673.1 .
AB029901 Genomic DNA. Translation: BAA89503.1 .
CR456350 mRNA. Translation: CAG30236.1 .
BC014649 mRNA. Translation: AAH14649.1 .
BC019077 mRNA. Translation: AAH19077.1 .
CCDSi CCDS13879.1.
RefSeqi NP_004852.1. NM_004861.1.
XP_005261905.1. XM_005261848.1.
XP_005261910.1. XM_005261853.1.
XP_005261913.1. XM_005261856.1.
UniGenei Hs.17958.

3D structure databases

ProteinModelPortali Q99999.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114891. 7 interactions.
STRINGi 9606.ENSP00000343234.

PTM databases

PhosphoSitei Q99999.

Proteomic databases

MaxQBi Q99999.
PaxDbi Q99999.
PRIDEi Q99999.

Protocols and materials databases

DNASUi 9514.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000338911 ; ENSP00000343234 ; ENSG00000128242 .
ENST00000401975 ; ENSP00000384388 ; ENSG00000128242 .
ENST00000402321 ; ENSP00000385735 ; ENSG00000128242 .
ENST00000402369 ; ENSP00000384122 ; ENSG00000128242 .
ENST00000406361 ; ENSP00000385207 ; ENSG00000128242 .
ENST00000406955 ; ENSP00000385825 ; ENSG00000128242 .
GeneIDi 9514.
KEGGi hsa:9514.
UCSCi uc003aig.1. human.

Organism-specific databases

CTDi 9514.
GeneCardsi GC22M030950.
HGNCi HGNC:24240. GAL3ST1.
HPAi HPA001220.
MIMi 602300. gene.
neXtProti NX_Q99999.
PharmGKBi PA134889661.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG45565.
GeneTreei ENSGT00390000013505.
HOGENOMi HOG000232148.
HOVERGENi HBG051681.
InParanoidi Q99999.
KOi K01019.
OMAi IQDSAMQ.
OrthoDBi EOG7K0ZF1.
PhylomeDBi Q99999.
TreeFami TF314802.

Enzyme and pathway databases

UniPathwayi UPA00222 .
BRENDAi 2.8.2.11. 2681.
Reactomei REACT_116105. Glycosphingolipid metabolism.

Miscellaneous databases

GeneWikii GAL3ST1.
GenomeRNAii 9514.
NextBioi 35650.
PROi Q99999.
SOURCEi Search...

Gene expression databases

Bgeei Q99999.
CleanExi HS_GAL3ST1.
ExpressionAtlasi Q99999. baseline and differential.
Genevestigatori Q99999.

Family and domain databases

InterProi IPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR14647. PTHR14647. 1 hit.
Pfami PF06990. Gal-3-0_sulfotr. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of cDNA encoding human 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase."
    Honke K., Tsuda M., Hirahara Y., Ishii A., Makita A., Wada Y.
    J. Biol. Chem. 272:4864-4868(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-14; 86-101; 102-105; 187-192; 278-286; 372-379 AND 416-423.
    Tissue: Renal cell carcinoma.
  2. "Cancer-associated alternative usage of multiple promoters of human GalCer sulfotransferase gene."
    Tsuda M., Egashira M., Niikawa N., Wada Y., Honke K.
    Eur. J. Biochem. 267:2672-2679(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Kidney proximal tubule.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29.
    Tissue: Kidney.
  5. "Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal cancer cells."
    Honke K., Yamane M., Ishii A., Kobayashi T., Makita A.
    J. Biochem. 119:421-427(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiG3ST1_HUMAN
AccessioniPrimary (citable) accession number: Q99999
Secondary accession number(s): Q96C63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3