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Q99999 (G3ST1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Galactosylceramide sulfotransferase

Short name=GalCer sulfotransferase
EC=2.8.2.11
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase
3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
Cerebroside sulfotransferase
Gene names
Name:GAL3ST1
Synonyms:CST
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes By similarity. Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro). Ref.5

Catalytic activity

3'-phosphoadenylyl sulfate + a galactosylceramide = adenosine 3',5'-bisphosphate + a galactosylceramidesulfate.

3'-phosphoadenylyl sulfate + monogalactosylalkylacylglycerol = adenosine 3',5'-bisphosphate + monogalactosylalkylacylglycerol sulfate.

Pathway

Lipid metabolism; sphingolipid metabolism.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Expressed in kidney proximal tubule, gastric mucosa and adenocarcinoma. Highly expressed in renal cell carcinoma cell lines.

Sequence similarities

Belongs to the galactose-3-O-sulfotransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Galactosylceramide sulfotransferase
PRO_0000085201

Regions

Topological domain1 – 1414Cytoplasmic Potential
Transmembrane15 – 3521Helical; Signal-anchor for type II membrane protein; Potential
Topological domain36 – 423388Lumenal Potential

Amino acid modifications

Glycosylation661N-linked (GlcNAc...) Potential
Glycosylation3121N-linked (GlcNAc...) Potential

Natural variations

Natural variant291V → M. Ref.3 Ref.4
Corresponds to variant rs2267161 [ dbSNP | Ensembl ].
VAR_013684

Experimental info

Sequence conflict281 – 2822RR → LN AA sequence Ref.1
Sequence conflict4181R → L AA sequence Ref.1
Sequence conflict4221R → E AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q99999 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 0FD4F2E374447C56

FASTA42348,764
        10         20         30         40         50         60 
MLPPQKKPWE SMAKGLVLGA LFTSFLLLVY SYAVPPLHAG LASTTPEAAA SCSPPALEPE 

        70         80         90        100        110        120 
AVIRANGSAG ECQPRRNIVF LKTHKTASST LLNILFRFGQ KHRLKFAFPN GRNDFDYPTF 

       130        140        150        160        170        180 
FARSLVQDYR PGACFNIICN HMRFHYDEVR GLVPTNAIFI TVLRDPARLF ESSFHYFGPV 

       190        200        210        220        230        240 
VPLTWKLSAG DKLTEFLQDP DRYYDPNGFN AHYLRNLLFF DLGYDNSLDP SSPQVQEHIL 

       250        260        270        280        290        300 
EVERRFHLVL LQEYFDESLV LLKDLLCWEL EDVLYFKLNA RRDSPVPRLS GELYGRATAW 

       310        320        330        340        350        360 
NMLDSHLYRH FNASFWRKVE AFGRERMARE VAALRHANER MRTICIDGGH AVDAAAIQDE 

       370        380        390        400        410        420 
AMQPWQPLGT KSILGYNLKK SIGQRHAQLC RRMLTPEIQY LMDLGANLWV TKLWKFIRDF 


LRW 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of cDNA encoding human 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase."
Honke K., Tsuda M., Hirahara Y., Ishii A., Makita A., Wada Y.
J. Biol. Chem. 272:4864-4868(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-14; 86-101; 102-105; 187-192; 278-286; 372-379 AND 416-423.
Tissue: Renal cell carcinoma.
[2]"Cancer-associated alternative usage of multiple promoters of human GalCer sulfotransferase gene."
Tsuda M., Egashira M., Niikawa N., Wada Y., Honke K.
Eur. J. Biochem. 267:2672-2679(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Kidney proximal tubule.
[3]"A genome annotation-driven approach to cloning the human ORFeome."
Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I.
Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29.
Tissue: Kidney.
[5]"Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal cancer cells."
Honke K., Yamane M., Ishii A., Kobayashi T., Makita A.
J. Biochem. 119:421-427(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D88667 mRNA. Translation: BAA13673.1.
AB029901 Genomic DNA. Translation: BAA89503.1.
CR456350 mRNA. Translation: CAG30236.1.
BC014649 mRNA. Translation: AAH14649.1.
BC019077 mRNA. Translation: AAH19077.1.
CCDSCCDS13879.1.
RefSeqNP_004852.1. NM_004861.1.
XP_005261905.1. XM_005261848.1.
XP_005261910.1. XM_005261853.1.
XP_005261913.1. XM_005261856.1.
UniGeneHs.17958.

3D structure databases

ProteinModelPortalQ99999.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114891. 1 interaction.
STRING9606.ENSP00000343234.

PTM databases

PhosphoSiteQ99999.

Proteomic databases

PaxDbQ99999.
PRIDEQ99999.

Protocols and materials databases

DNASU9514.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000338911; ENSP00000343234; ENSG00000128242.
ENST00000401975; ENSP00000384388; ENSG00000128242.
ENST00000402321; ENSP00000385735; ENSG00000128242.
ENST00000402369; ENSP00000384122; ENSG00000128242.
ENST00000406361; ENSP00000385207; ENSG00000128242.
ENST00000406955; ENSP00000385825; ENSG00000128242.
ENST00000443111; ENSP00000402587; ENSG00000128242.
GeneID9514.
KEGGhsa:9514.
UCSCuc003aig.1. human.

Organism-specific databases

CTD9514.
GeneCardsGC22M030950.
HGNCHGNC:24240. GAL3ST1.
HPAHPA001220.
MIM602300. gene.
neXtProtNX_Q99999.
PharmGKBPA134889661.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG45565.
HOGENOMHOG000232148.
HOVERGENHBG051681.
InParanoidQ99999.
KOK01019.
OMAIQDSAMQ.
OrthoDBEOG7K0ZF1.
PhylomeDBQ99999.
TreeFamTF314802.

Enzyme and pathway databases

BRENDA2.8.2.11. 2681.
ReactomeREACT_111217. Metabolism.
UniPathwayUPA00222.

Gene expression databases

ArrayExpressQ99999.
BgeeQ99999.
CleanExHS_GAL3ST1.
GenevestigatorQ99999.

Family and domain databases

InterProIPR009729. Gal-3-0_sulfotransfrase.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR14647. PTHR14647. 1 hit.
PfamPF06990. Gal-3-0_sulfotr. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

GeneWikiGAL3ST1.
GenomeRNAi9514.
NextBio35650.
PROQ99999.
SOURCESearch...

Entry information

Entry nameG3ST1_HUMAN
AccessionPrimary (citable) accession number: Q99999
Secondary accession number(s): Q96C63
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 22

Human chromosome 22: entries, gene names and cross-references to MIM