Reviewed,
UniProtKB/Swiss-Prot Q99999 (G3ST1_HUMAN)
Last modified
November 3, 2009.
Version 78.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (6) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Galactosylceramide sulfotransferase Short name=GalCer sulfotransferase EC=2.8.2.11 Alternative name(s): Cerebroside sulfotransferase 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 423 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes By similarity. Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro). |
| Catalytic activity | 3'-phosphoadenylyl sulfate + a galactosylceramide = adenosine 3',5'-bisphosphate + a galactosylceramidesulfate. 3'-phosphoadenylyl sulfate + monogalactosylalkylacylglycerol = adenosine 3',5'-bisphosphate + monogalactosylalkylacylglycerol sulfate. |
| Pathway | |
| Subcellular location | Golgi apparatus membrane; Single-pass type II membrane protein By similarity. |
| Tissue specificity | Expressed in kidney proximal tubule, gastric mucosa and adenocarcinoma. Highly expressed in renal cell carcinoma cell lines. |
| Sequence similarities | Belongs to the galactose-3-O-sulfotransferase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Golgi apparatus Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Signal-anchor Transmembrane |
| Molecular function | Transferase |
| PTM | Glycoprotein |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | protein amino acid N-linked glycosylation Ref.1 Traceable author statement. Source: ProtInc |
| Cellular component | Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Ref.1Traceable author statement. Source: ProtInc membrane fraction Ref.1Traceable author statement. Source: ProtInc |
| Molecular function | galactosylceramide sulfotransferase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 423 | 423 | Galactosylceramide sulfotransferase | PRO_0000085201 | |||||
Regions | |||||||||
| Topological domain | 1 – 14 | 14 | Cytoplasmic Potential | ||||||
| Transmembrane | 15 – 35 | 21 | Signal-anchor for type II membrane protein Potential | ||||||
| Topological domain | 36 – 423 | 388 | Lumenal Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 66 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 312 | 1 | N-linked (GlcNAc...) Potential | ||||||
Natural variations | |||||||||
| Natural variant | 29 | 1 | V → M: dbSNP rs2267161. Ref.3 Ref.4 | VAR_013684 | |||||
Experimental info | |||||||||
| Sequence conflict | 281 – 282 | 2 | RR → LN AA sequence Ref.1 | ||||||
| Sequence conflict | 418 | 1 | R → L AA sequence Ref.1 | ||||||
| Sequence conflict | 422 | 1 | R → E AA sequence Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning and expression of cDNA encoding human 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase." Honke K., Tsuda M., Hirahara Y., Ishii A., Makita A., Wada Y. J. Biol. Chem. 272:4864-4868(1997) [PubMed: 9030544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-14; 86-101; 102-105; 187-192; 278-286; 372-379 AND 416-423. Tissue: Renal cell carcinoma. |
| [2] | "Cancer-associated alternative usage of multiple promoters of human GalCer sulfotransferase gene." Tsuda M., Egashira M., Niikawa N., Wada Y., Honke K. Eur. J. Biochem. 267:2672-2679(2000) [PubMed: 10785389] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Kidney proximal tubule. |
| [3] | "A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29. Tissue: Kidney. |
| [5] | "Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal cancer cells." Honke K., Yamane M., Ishii A., Kobayashi T., Makita A. J. Biochem. 119:421-427(1996) [PubMed: 8830034] [Abstract] Cited for: FUNCTION. |
| + | Additional computationally mapped references. |
Cross-references
Entry information
| Entry name | G3ST1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q99999 Secondary accession number(s): Q96C63 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 22 Human chromosome 22: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
