Q99999 (G3ST1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactosylceramide sulfotransferase
Short name=GalCer sulfotransferase
EC=2.8.2.11

Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase
3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
Cerebroside sulfotransferase

Gene names

Name:	GAL3ST1
Synonyms:	CST

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes By similarity. Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro). Ref.5
Catalytic activity	3'-phosphoadenylyl sulfate + a galactosylceramide = adenosine 3',5'-bisphosphate + a galactosylceramidesulfate. 3'-phosphoadenylyl sulfate + monogalactosylalkylacylglycerol = adenosine 3',5'-bisphosphate + monogalactosylalkylacylglycerol sulfate.
Pathway	Lipid metabolism; sphingolipid metabolism.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Expressed in kidney proximal tubule, gastric mucosa and adenocarcinoma. Highly expressed in renal cell carcinoma cell lines.
Sequence similarities	Belongs to the galactose-3-O-sulfotransferase family.

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	galactosylceramide biosynthetic process Inferred from electronic annotation. Source: Compara glycosphingolipid metabolic process Traceable author statement. Source: Reactome myelination Inferred from electronic annotation. Source: Compara phospholipid metabolic process Traceable author statement. Source: Reactome protein N-linked glycosylation Traceable author statement Ref.1. Source: ProtInc small molecule metabolic process Traceable author statement. Source: Reactome spermatogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	galactosylceramide sulfotransferase activity Inferred from electronic annotation. Source: EC sulfotransferase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Galactosylceramide sulfotransferase		PRO_0000085201
Regions
	Topological domain	1 – 14	14	Cytoplasmic Potential
	Transmembrane	15 – 35	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	36 – 423	388	Lumenal Potential
Amino acid modifications
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	312	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	29	1	V → M. Ref.3 Ref.4 Corresponds to variant rs2267161 [ dbSNP | Ensembl ].		VAR_013684
Experimental info
	Sequence conflict	281 – 282	2	RR → LN AA sequence Ref.1
	Sequence conflict	418	1	R → L AA sequence Ref.1
	Sequence conflict	422	1	R → E AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99999 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 0FD4F2E374447C56	FASTA	423	48,764
	10 20 30 40 50 60 MLPPQKKPWE SMAKGLVLGA LFTSFLLLVY SYAVPPLHAG LASTTPEAAA SCSPPALEPE 70 80 90 100 110 120 AVIRANGSAG ECQPRRNIVF LKTHKTASST LLNILFRFGQ KHRLKFAFPN GRNDFDYPTF 130 140 150 160 170 180 FARSLVQDYR PGACFNIICN HMRFHYDEVR GLVPTNAIFI TVLRDPARLF ESSFHYFGPV 190 200 210 220 230 240 VPLTWKLSAG DKLTEFLQDP DRYYDPNGFN AHYLRNLLFF DLGYDNSLDP SSPQVQEHIL 250 260 270 280 290 300 EVERRFHLVL LQEYFDESLV LLKDLLCWEL EDVLYFKLNA RRDSPVPRLS GELYGRATAW 310 320 330 340 350 360 NMLDSHLYRH FNASFWRKVE AFGRERMARE VAALRHANER MRTICIDGGH AVDAAAIQDE 370 380 390 400 410 420 AMQPWQPLGT KSILGYNLKK SIGQRHAQLC RRMLTPEIQY LMDLGANLWV TKLWKFIRDF LRW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of cDNA encoding human 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase." Honke K., Tsuda M., Hirahara Y., Ishii A., Makita A., Wada Y. J. Biol. Chem. 272:4864-4868(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-14; 86-101; 102-105; 187-192; 278-286; 372-379 AND 416-423. Tissue: Renal cell carcinoma.
[2]	"Cancer-associated alternative usage of multiple promoters of human GalCer sulfotransferase gene." Tsuda M., Egashira M., Niikawa N., Wada Y., Honke K. Eur. J. Biochem. 267:2672-2679(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Kidney proximal tubule.
[3]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29. Tissue: Kidney.
[5]	"Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal cancer cells." Honke K., Yamane M., Ishii A., Kobayashi T., Makita A. J. Biochem. 119:421-427(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	D88667 mRNA. Translation: BAA13673.1. AB029901 Genomic DNA. Translation: BAA89503.1. CR456350 mRNA. Translation: CAG30236.1. BC014649 mRNA. Translation: AAH14649.1. BC019077 mRNA. Translation: AAH19077.1.
IPI	IPI00006817.
RefSeq	NP_004852.1. NM_004861.1.
UniGene	Hs.17958.
3D structure databases
ProteinModelPortal	Q99999.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000343234.
PTM databases
PhosphoSite	Q99999.
Polymorphism databases
DMDM	22001556.
Proteomic databases
PaxDb	Q99999.
PRIDE	Q99999.
Protocols and materials databases
DNASU	9514.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338911; ENSP00000343234; ENSG00000128242. ENST00000401975; ENSP00000384388; ENSG00000128242. ENST00000402321; ENSP00000385735; ENSG00000128242. ENST00000402369; ENSP00000384122; ENSG00000128242. ENST00000406361; ENSP00000385207; ENSG00000128242. ENST00000406955; ENSP00000385825; ENSG00000128242. ENST00000443111; ENSP00000402587; ENSG00000128242.
GeneID	9514.
KEGG	hsa:9514.
UCSC	uc003aig.1. human.
Organism-specific databases
CTD	9514.
GeneCards	GC22M030950.
HGNC	HGNC:24240. GAL3ST1.
HPA	HPA001220.
MIM	602300. gene.
neXtProt	NX_Q99999.
PharmGKB	PA134889661.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG45565.
HOGENOM	HOG000232148.
HOVERGEN	HBG051681.
InParanoid	Q99999.
KO	K01019.
OMA	DRYYDPN.
OrthoDB	EOG48D0VH.
PhylomeDB	Q99999.
Enzyme and pathway databases
BRENDA	2.8.2.11. 2681.
Reactome	REACT_111217. Metabolism.
UniPathway	UPA00222.
Gene expression databases
ArrayExpress	Q99999.
Bgee	Q99999.
CleanEx	HS_GAL3ST1.
Genevestigator	Q99999.
GermOnline	ENSG00000128242. Homo sapiens.
Family and domain databases
InterPro	IPR009729. Gal-3-0_sulfotransfrase. [Graphical view]
PANTHER	PTHR14647. PTHR14647. 1 hit.
Pfam	PF06990. Gal-3-0_sulfotr. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9514.
NextBio	35650.
SOURCE	Search...

Entry information

Entry name

G3ST1_HUMAN

Accession

Primary (citable) accession number: Q99999
Secondary accession number(s): Q96C63

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.