Reviewed,
UniProtKB/Swiss-Prot Q99986 (VRK1_HUMAN)
Last modified
June 16, 2009.
Version 82.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine/threonine-protein kinase VRK1 EC=2.7.11.1 Alternative name(s): Vaccinia-related kinase 1 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 396 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Serine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Ref.3 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Enzyme regulation | Active in presence of Mn2+, Mg2+ and Zn2+, but is not functional with Ca2+ or Cu2+. Has a higher affinity for Mn2+ than for Mg2+. Ref.4 |
| Subcellular location | Nucleus. Note: Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis. Ref.3 |
| Tissue specificity | Widely expressed. Highly expressed in fetal liver, testis and thymus. Ref.1 |
| Post-translational modification | Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53. Ref.3 Ref.4 |
| Sequence similarities | Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Nucleus |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Cellular component | cytoplasm Inferred from direct assay. Source: HPA nucleusInferred from direct assay. Source: HPA |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.3Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Ref.1Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ATF2 | P15336 | 1 | EBI-1769146,EBI-1170906 | |
| JUN | P05412 | 3 | EBI-1769146,EBI-852823 | |
| PLK3 | Q9H4B4 | 6 | EBI-1769146,EBI-751877 | |
| RAN | P62826 | 4 | EBI-1769146,EBI-286642 | |
| Tp53 | P02340 | 1 | EBI-1769146,EBI-474016 | From a different organism. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 396 | 396 | Serine/threonine-protein kinase VRK1 | PRO_0000086803 | |||||
Regions | |||||||||
| Domain | 37 – 317 | 281 | Protein kinase | ||||||
| Nucleotide binding | 43 – 51 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 177 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 71 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 355 | 1 | Phosphothreonine; by autocatalysis | ||||||
Experimental info | |||||||||
| Mutagenesis | 14 | 1 | S → A: Does not abolish autophosphorylation. Ref.3 | ||||||
| Mutagenesis | 102 | 1 | T → A: Does not abolish autophosphorylation. Ref.3 | ||||||
| Mutagenesis | 125 | 1 | S → A: Does not abolish autophosphorylation. Ref.3 | ||||||
| Mutagenesis | 150 | 1 | S → A: Does not abolish autophosphorylation. Ref.3 | ||||||
| Mutagenesis | 158 | 1 | S → A: Does not abolish autophosphorylation. Ref.3 | ||||||
| Mutagenesis | 239 | 1 | S → A: Does not abolish autophosphorylation. Ref.3 | ||||||
| Mutagenesis | 305 | 1 | T → A: Does not abolish autophosphorylation. Ref.3 | ||||||
| Mutagenesis | 312 | 1 | T → A: Does not abolish autophosphorylation. Ref.3 | ||||||
| Mutagenesis | 355 | 1 | T → A: Does not abolish autophosphorylation. Ref.3 | ||||||
| Mutagenesis | 390 | 1 | T → A: Does not abolish autophosphorylation. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of two novel human putative serine/threonine kinases, VRK1 and VRK2, with structural similarity to Vaccinia virus B1R kinase." Nezu J., Oku A., Jones M.H., Shimane M. Genomics 45:327-331(1997) [PubMed: 9344656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Fetal liver. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Lung. |
| [3] | "The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein." Lopez-Borges S., Lazo P.A. Oncogene 19:3656-3664(2000) [PubMed: 10951572] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION, MUTAGENESIS OF SER-14; THR-102; SER-125; SER-150; SER-158; SER-239; THR-305; THR-312; THR-355 AND THR-390. |
| [4] | "Kinetic properties of p53 phosphorylation by the human vaccinia-related kinase 1." Barcia R., Lopez-Borges S., Vega F.M., Lazo P.A. Arch. Biochem. Biophys. 399:1-5(2002) [PubMed: 11883897] [Abstract] Cited for: AUTOPHOSPHORYLATION, ENZYME REGULATION. |
| [5] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AB000449 mRNA. Translation: BAA19108.1. BC103761 mRNA. Translation: AAI03762.1. BC112075 mRNA. Translation: AAI12076.1. BC113510 mRNA. Translation: AAI13511.1. | |
| IPI | IPI00019640. |
| RefSeq | NP_003375.1. |
| UniGene | Hs.422662 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1CKI based on UniProtKB Q06486. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q99986. 11 interactions. |
PTM databases | |
| PhosphoSite | Q99986. |
Proteomic databases | |
| PeptideAtlas | Q99986. |
| PRIDE | Q99986. |
Genome annotation databases | |
| Ensembl | ENSG00000100749. Homo sapiens. [Contig view] |
| GeneID | 7443. |
| KEGG | hsa:7443. |
Organism-specific databases | |
| GeneCards | GC14P096333. |
| H-InvDB | HIX0037640. |
| HGNC | HGNC:12718. VRK1. |
| HPA | HPA000660. |
| MIM | 602168. gene. |
| PharmGKB | PA37330. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q99986. |
| HOVERGEN | Q99986. |
| OMA | Q99986. NKPGEIA. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 247. |
Gene expression databases | |
| ArrayExpress | Q99986. |
| Bgee | Q99986. |
| CleanEx | HS_VRK1. |
| GermOnline | ENSG00000100749. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser_thr_pkin_AS. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 29150. |
| SOURCE | Search... |
Entry information
| Entry name | VRK1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q99986 Secondary accession number(s): Q3SYL2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 14 Human chromosome 14: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


