Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Disintegrin and metalloproteinase domain-containing protein 2

Gene

ADAM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein.

GO - Molecular functioni

  • integrin binding Source: ProtInc
  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104755-MONOMER.
ReactomeiR-HSA-1300644. Interaction With The Zona Pellucida.
SIGNORiQ99965.

Protein family/group databases

MEROPSiM12.950.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 2
Short name:
ADAM 2
Alternative name(s):
Cancer/testis antigen 15
Short name:
CT15
Fertilin subunit beta
PH-30
Short name:
PH30
PH30-beta
Gene namesi
Name:ADAM2
Synonyms:FTNB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:198. ADAM2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini175 – 686ExtracellularSequence analysisAdd BLAST512
Transmembranei687 – 707HelicalSequence analysisAdd BLAST21
Topological domaini708 – 735CytoplasmicSequence analysisAdd BLAST28

GO - Cellular componenti

  • cell surface Source: Ensembl
  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi2515.
OpenTargetsiENSG00000104755.
ENSG00000276286.
PharmGKBiPA24515.

Polymorphism and mutation databases

DMDMi28202251.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
PropeptideiPRO_000002904217 – 174Add BLAST158
ChainiPRO_0000029043175 – 735Disintegrin and metalloproteinase domain-containing protein 2Add BLAST561

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi122N-linked (GlcNAc...)Sequence analysis1
Glycosylationi220N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi287 ↔ 370By similarity
Disulfide bondi329 ↔ 354By similarity
Disulfide bondi331 ↔ 336By similarity
Glycosylationi353N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi445 ↔ 465By similarity
Glycosylationi459N-linked (GlcNAc...)Sequence analysis1
Glycosylationi566N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi616 ↔ 627By similarity
Disulfide bondi621 ↔ 633By similarity
Disulfide bondi635 ↔ 644By similarity
Modified residuei729PhosphoserineBy similarity1

Post-translational modificationi

The prodomain and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ99965.
PeptideAtlasiQ99965.
PRIDEiQ99965.

2D gel databases

REPRODUCTION-2DPAGEQ99965.

PTM databases

iPTMnetiQ99965.
PhosphoSitePlusiQ99965.

Expressioni

Tissue specificityi

Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues.

Gene expression databases

BgeeiENSG00000104755.
CleanExiHS_ADAM2.
ExpressionAtlasiQ99965. baseline and differential.
GenevisibleiQ99965. HS.

Organism-specific databases

HPAiCAB022390.
HPA024621.
HPA026581.

Interactioni

GO - Molecular functioni

  • integrin binding Source: ProtInc

Protein-protein interaction databases

BioGridi108791. 10 interactors.
STRINGi9606.ENSP00000265708.

Structurei

3D structure databases

ProteinModelPortaliQ99965.
SMRiQ99965.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 375Peptidase M12BPROSITE-ProRule annotationAdd BLAST198
Domaini384 – 473DisintegrinPROSITE-ProRule annotationAdd BLAST90
Domaini612 – 645EGF-likeAdd BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi477 – 606Cys-richAdd BLAST130

Domaini

A tripeptide motif (FEE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.Curated
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00800000124044.
HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiQ99965.
KOiK06833.
OMAiCGKLICK.
OrthoDBiEOG091G02F0.
PhylomeDBiQ99965.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR033958. ADAM2.
IPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PANTHERiPTHR11905:SF108. PTHR11905:SF108. 1 hit.
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99965-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWRVLFLLSG LGGLRMDSNF DSLPVQITVP EKIRSIIKEG IESQASYKIV
60 70 80 90 100
IEGKPYTVNL MQKNFLPHNF RVYSYSGTGI MKPLDQDFQN FCHYQGYIEG
110 120 130 140 150
YPKSVVMVST CTGLRGVLQF ENVSYGIEPL ESSVGFEHVI YQVKHKKADV
160 170 180 190 200
SLYNEKDIES RDLSFKLQSV EPQQDFAKYI EMHVIVEKQL YNHMGSDTTV
210 220 230 240 250
VAQKVFQLIG LTNAIFVSFN ITIILSSLEL WIDENKIATT GEANELLHTF
260 270 280 290 300
LRWKTSYLVL RPHDVAFLLV YREKSNYVGA TFQGKMCDAN YAGGVVLHPR
310 320 330 340 350
TISLESLAVI LAQLLSLSMG ITYDDINKCQ CSGAVCIMNP EAIHFSGVKI
360 370 380 390 400
FSNCSFEDFA HFISKQKSQC LHNQPRLDPF FKQQAVCGNA KLEAGEECDC
410 420 430 440 450
GTEQDCALIG ETCCDIATCR FKAGSNCAEG PCCENCLFMS KERMCRPSFE
460 470 480 490 500
ECDLPEYCNG SSASCPENHY VQTGHPCGLN QWICIDGVCM SGDKQCTDTF
510 520 530 540 550
GKEVEFGPSE CYSHLNSKTD VSGNCGISDS GYTQCEADNL QCGKLICKYV
560 570 580 590 600
GKFLLQIPRA TIIYANISGH LCIAVEFASD HADSQKMWIK DGTSCGSNKV
610 620 630 640 650
CRNQRCVSSS YLGYDCTTDK CNDRGVCNNK KHCHCSASYL PPDCSVQSDL
660 670 680 690 700
WPGGSIDSGN FPPVAIPARL PERRYIENIY HSKPMRWPFF LFIPFFIIFC
710 720 730
VLIAIMVKVN FQRKKWRTED YSSDEQPESE SEPKG
Length:735
Mass (Da):82,457
Last modified:February 1, 2003 - v2
Checksum:i92867B5340BEE01F
GO
Isoform 2 (identifier: Q99965-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     172-190: Missing.

Note: No experimental confirmation available.
Show »
Length:716
Mass (Da):80,158
Checksum:iA25CA5AD8AE5A3C6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3Missing in AAD04206 (PubMed:8702389).Curated1
Sequence conflicti21D → H in CAA67753 (PubMed:9070941).Curated1
Sequence conflicti99E → D in CAA67753 (PubMed:9070941).Curated1
Sequence conflicti106V → G in CAA67753 (PubMed:9070941).Curated1
Sequence conflicti170V → A in AAD04206 (PubMed:8702389).Curated1
Sequence conflicti288D → H in AAC51110 (PubMed:9041139).Curated1
Sequence conflicti321I → T in AAC51110 (PubMed:9041139).Curated1
Sequence conflicti388G → S in CAA67753 (PubMed:9070941).Curated1
Sequence conflicti396 – 398EEC → DEF in CAA67753 (PubMed:9070941).Curated3
Sequence conflicti501G → S in CAA67753 (PubMed:9070941).Curated1
Sequence conflicti529D → Y in CAA67753 (PubMed:9070941).Curated1
Sequence conflicti579S → G in CAA67753 (PubMed:9070941).Curated1
Sequence conflicti588W → L in CAA67753 (PubMed:9070941).Curated1
Sequence conflicti603N → D in CAA67753 (PubMed:9070941).Curated1
Sequence conflicti629 – 630NK → KQ in CAA67753 (PubMed:9070941).Curated2
Sequence conflicti638S → F in CAA67753 (PubMed:9070941).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03521710G → W.Corresponds to variant rs34800519dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005471172 – 190Missing in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52370 mRNA. Translation: AAC51110.1.
U38805 mRNA. Translation: AAD04206.1.
X99374 mRNA. Translation: CAA67753.1.
AJ133005 mRNA. Translation: CAB40813.1.
BC034957 mRNA. Translation: AAH34957.1.
CCDSiCCDS34884.1. [Q99965-1]
CCDS64882.1. [Q99965-2]
PIRiJC4861.
RefSeqiNP_001265042.1. NM_001278113.1. [Q99965-2]
NP_001265043.1. NM_001278114.1.
NP_001455.3. NM_001464.4. [Q99965-1]
UniGeneiHs.177959.

Genome annotation databases

EnsembliENST00000265708; ENSP00000265708; ENSG00000104755. [Q99965-1]
ENST00000347580; ENSP00000343854; ENSG00000104755. [Q99965-2]
ENST00000613160; ENSP00000484999; ENSG00000276286. [Q99965-2]
ENST00000620181; ENSP00000482337; ENSG00000276286. [Q99965-1]
GeneIDi2515.
KEGGihsa:2515.
UCSCiuc003xnj.5. human. [Q99965-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52370 mRNA. Translation: AAC51110.1.
U38805 mRNA. Translation: AAD04206.1.
X99374 mRNA. Translation: CAA67753.1.
AJ133005 mRNA. Translation: CAB40813.1.
BC034957 mRNA. Translation: AAH34957.1.
CCDSiCCDS34884.1. [Q99965-1]
CCDS64882.1. [Q99965-2]
PIRiJC4861.
RefSeqiNP_001265042.1. NM_001278113.1. [Q99965-2]
NP_001265043.1. NM_001278114.1.
NP_001455.3. NM_001464.4. [Q99965-1]
UniGeneiHs.177959.

3D structure databases

ProteinModelPortaliQ99965.
SMRiQ99965.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108791. 10 interactors.
STRINGi9606.ENSP00000265708.

Protein family/group databases

MEROPSiM12.950.

PTM databases

iPTMnetiQ99965.
PhosphoSitePlusiQ99965.

Polymorphism and mutation databases

DMDMi28202251.

2D gel databases

REPRODUCTION-2DPAGEQ99965.

Proteomic databases

PaxDbiQ99965.
PeptideAtlasiQ99965.
PRIDEiQ99965.

Protocols and materials databases

DNASUi2515.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265708; ENSP00000265708; ENSG00000104755. [Q99965-1]
ENST00000347580; ENSP00000343854; ENSG00000104755. [Q99965-2]
ENST00000613160; ENSP00000484999; ENSG00000276286. [Q99965-2]
ENST00000620181; ENSP00000482337; ENSG00000276286. [Q99965-1]
GeneIDi2515.
KEGGihsa:2515.
UCSCiuc003xnj.5. human. [Q99965-1]

Organism-specific databases

CTDi2515.
DisGeNETi2515.
GeneCardsiADAM2.
H-InvDBHIX0025571.
HGNCiHGNC:198. ADAM2.
HPAiCAB022390.
HPA024621.
HPA026581.
MIMi601533. gene.
neXtProtiNX_Q99965.
OpenTargetsiENSG00000104755.
ENSG00000276286.
PharmGKBiPA24515.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00800000124044.
HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiQ99965.
KOiK06833.
OMAiCGKLICK.
OrthoDBiEOG091G02F0.
PhylomeDBiQ99965.
TreeFamiTF314733.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104755-MONOMER.
ReactomeiR-HSA-1300644. Interaction With The Zona Pellucida.
SIGNORiQ99965.

Miscellaneous databases

GeneWikiiADAM2.
GenomeRNAii2515.
PROiQ99965.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000104755.
CleanExiHS_ADAM2.
ExpressionAtlasiQ99965. baseline and differential.
GenevisibleiQ99965. HS.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR033958. ADAM2.
IPR006586. ADAM_Cys-rich.
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PANTHERiPTHR11905:SF108. PTHR11905:SF108. 1 hit.
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADAM2_HUMAN
AccessioniPrimary (citable) accession number: Q99965
Secondary accession number(s): P78326, Q9UQQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 2003
Last modified: November 30, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In mammals, exists as a heterodimer composed of an alpha and beta subunits. In human, fertilin subunit alpha is a pseudogene.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.